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Servicebio Inc rapid blocking solution
Rapid Blocking Solution, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rapid+blocking+solution/blocking+solution/pm42061695-76-4-7
Average 86 stars, based on 1 article reviews
rapid blocking solution - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Blocking Assay:

Article Title: Anti-Ulcerative Colitis Tanzawaic Acids from a Marine Algicolous Fungus, Penicillium steckii SCSIO 41040
Article Snippet: .. Subsequently, the blocking solution was removed, and the cells were incubated overnight at 4 °C with the occludin (Servicebio, GB111401-100, 1:200) and ZO-1 (Servicebio, GB111402-100, 1:200) primary antibody. ..

Article Title: Quercetin suppresses the progression of HBV-associated hepatocellular carcinoma by modulating the EGFR signaling pathway.
Article Snippet: Equal amounts of protein were denatured and separated by 8–12% SDS-PAGE, then transferred to PVDF membranes (Merck Millipore, ISEQ00010). .. The membranes were blocked with a blocking solution (Servicebio, CR2405137) for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C. ..

Article Title: Dachaihu decoction alleviates septic liver injury by modulating the intestinal barrier dysfunction and suppressing the NF-κB/NLRP3/Caspase-1 signaling pathway.
Article Snippet: The protein samples were separated by 8–15% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010, Germany). .. Membranes were blocked for 30 min with quick blocking buffer (G2052; Servicebio, Wuhan, China), followed by overnight incubation at 4 °C with the following primary antibodies: zonula occludens-1 (ZO-1) (rabbit, 1:2000, 21773-1-AP; Proteintech, Wuhan, China), E-cadherin (rabbit, 1:2000, 20874-1-AP; Proteintech, Wuhan, China), Occludin (rabbit, 1:2000, 27260-1-AP; Proteintech, Wuhan, China), TLR4 (mouse, 1:1000), MyD88 (mouse; 1:1000), IκBα (mouse, 1:2000, #66418-1-lg; Proteintech, Wuhan, China), p-IκBα (rabbit, 1:500, #11152; Signalway Antibody, USA), NF-κB p65 (mouse; 1:1000), p-NF-κB p65 (rabbit; 1:1000; #ab86299, Abcam, USA), NLRP3 (rabbit, 1:1000, #A21906; ABclonal, Wuhan, China), apoptosis- associated speck-like protein containing a CARD (ASC) (rabbit, 1:1000, #A16672; ABclonal, Wuhan, China), Cysteine-aspartic acid protease 1 (Caspase-1) (rabbit, 1:1000, #A0964; ABclonal, Wuhan, China), Cleaved Caspase-1 (rabbit, 1:1000, #89332; CST, USA); IL-1β (rabbit, 1:1000, #31202; CST, USA), β-actin (rabbit, 1:3000, 81115-1-RR; Proteintech, Wuhan, China). .. The membranes were incubated for 1 h at room temperature with species-matched horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse/rabbit IgG, 1:4000, SA00001-1, SA00001-2; Proteintech, Wuhan, China).

Article Title: Acetyltransferase p300 Promotes NNK-Induced Colorectal Cancer Progression by Mediating ANKHD1 Expression via H3K27ac Modification.
Article Snippet: Cigarette smoking significantly accelerates the initiation and progression of colorectal cancer (CRC), although the precise molecular mechanisms remain incompletely elucidated.. Among tobacco-derived carcinogens, 4(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a key one, which has been demonstrated to enhance the malignant progression of CRC.. CUT&RUN-seq and mRNA-seq analyses, along with subsequent validation experiments, reveal that NNK upregulates the expression of the acetyltransferase p300.

Article Title: The role of R-loop aberrations in lower-grade gliomas: prognostic, immune, and metabolic implications from multi-omics and machine learning analysis
Article Snippet: Equal amounts of protein were mixed with loading buffer (Cat. No. P0015, Beyotime), boiled at 98 °C for 10 min, separated on 7.5% SDS-PAGE gels (Cat. No. PG111, Epizyme) and transferred to 0.22 μm polyvinylidene fluoride membranes (Millipore). .. Membranes were blocked with Fast Blocking Fluid (Cat. No. G2052, Servicebio) for 15 min at room temperature, incubated overnight at 4 °C with primary antibodies, washed with 0.1% TBST, and incubated with secondary antibodies for 1 h. Signals were detected using a Tanon imaging system and quantified with ImageJ. .. The primary antibodies were: anti-INCENP (Cat. No. 30388, ProMab), anti-BAX (Cat.No.

Article Title: YBX1 Confers immunosuppressive bone metastatic traits in non-small cell lung cancer.
Article Snippet: Subsequently, the sections were treated with antigen retrieval buffer (Servicebio, G1202) and 3% hydrogen peroxide at room temperature for 25 minutes protected from light. .. A blocking solution of 3% bovine serum albumin (Servicebio, GC305010) was applied and incubated at room temperature for 30 minutes. .. The sections were then incubated overnight at 4°C with the following primary antibodies, respectively, followed by three washes with PBS buffer (pH 7.4): FOXP3 (iF546-Tyramide, Proteintech, 65089-1-Ig, 1:500), CD4 (iF594-Tyramide, Proteintech, 86300-3-RR, 1:500), IFN-γ (iF647-Tyramide, Proteintech, 29788-1-AP, 1:500), YBX1 (iF700-Tyramide, Proteintech, 20339-1-AP, 1:400), CD8 (iF488Tyramide, Proteintech, 85977-4-RR, 1:500), and CD25 (iF440-Tyramide, CST, 39475S, 1:200).

Article Title: Dietary riboflavin blocks the cGAS-STING pathway to curb hypoxia-induced hepatic inflammation in sub-adult grass carp (Ctenopharyngodon idella)
Article Snippet: .. The membranes were blocked with blocking solution (G2052-210 Jo urn al Pr e-p roo f 500 ml, Servicebio Biotechnology Co., Ltd., Wuhan, Hubei, China) and then incubated 211 overnight at 4 °C with primary antibodies. .. Subsequently, the membranes were exposed 212 to the secondary antibody for 1.5 h. The bands were detected using an ECL kit (PD203-213 500 ml, Oriscience Biotechnology Co., Ltd., Chengdu, Sichuan, China), imaged using 214 the multimodal imaging system (ChemiDoc, Bio-Rad Laboratories, Inc., USA), and 215 analyzed with ImageJ software (1.42q).

Incubation:

Article Title: Anti-Ulcerative Colitis Tanzawaic Acids from a Marine Algicolous Fungus, Penicillium steckii SCSIO 41040
Article Snippet: .. Subsequently, the blocking solution was removed, and the cells were incubated overnight at 4 °C with the occludin (Servicebio, GB111401-100, 1:200) and ZO-1 (Servicebio, GB111402-100, 1:200) primary antibody. ..

Article Title: Quercetin suppresses the progression of HBV-associated hepatocellular carcinoma by modulating the EGFR signaling pathway.
Article Snippet: Equal amounts of protein were denatured and separated by 8–12% SDS-PAGE, then transferred to PVDF membranes (Merck Millipore, ISEQ00010). .. The membranes were blocked with a blocking solution (Servicebio, CR2405137) for 1 h at room temperature and incubated with primary antibodies overnight at 4 °C. ..

Article Title: Dachaihu decoction alleviates septic liver injury by modulating the intestinal barrier dysfunction and suppressing the NF-κB/NLRP3/Caspase-1 signaling pathway.
Article Snippet: The protein samples were separated by 8–15% SDS-PAGE and transferred to polyvinylidene fluoride (PVDF) membranes (Millipore, ISEQ00010, Germany). .. Membranes were blocked for 30 min with quick blocking buffer (G2052; Servicebio, Wuhan, China), followed by overnight incubation at 4 °C with the following primary antibodies: zonula occludens-1 (ZO-1) (rabbit, 1:2000, 21773-1-AP; Proteintech, Wuhan, China), E-cadherin (rabbit, 1:2000, 20874-1-AP; Proteintech, Wuhan, China), Occludin (rabbit, 1:2000, 27260-1-AP; Proteintech, Wuhan, China), TLR4 (mouse, 1:1000), MyD88 (mouse; 1:1000), IκBα (mouse, 1:2000, #66418-1-lg; Proteintech, Wuhan, China), p-IκBα (rabbit, 1:500, #11152; Signalway Antibody, USA), NF-κB p65 (mouse; 1:1000), p-NF-κB p65 (rabbit; 1:1000; #ab86299, Abcam, USA), NLRP3 (rabbit, 1:1000, #A21906; ABclonal, Wuhan, China), apoptosis- associated speck-like protein containing a CARD (ASC) (rabbit, 1:1000, #A16672; ABclonal, Wuhan, China), Cysteine-aspartic acid protease 1 (Caspase-1) (rabbit, 1:1000, #A0964; ABclonal, Wuhan, China), Cleaved Caspase-1 (rabbit, 1:1000, #89332; CST, USA); IL-1β (rabbit, 1:1000, #31202; CST, USA), β-actin (rabbit, 1:3000, 81115-1-RR; Proteintech, Wuhan, China). .. The membranes were incubated for 1 h at room temperature with species-matched horseradish peroxidase (HRP)-conjugated secondary antibodies (anti-mouse/rabbit IgG, 1:4000, SA00001-1, SA00001-2; Proteintech, Wuhan, China).

Article Title: Acetyltransferase p300 Promotes NNK-Induced Colorectal Cancer Progression by Mediating ANKHD1 Expression via H3K27ac Modification.
Article Snippet: Cigarette smoking significantly accelerates the initiation and progression of colorectal cancer (CRC), although the precise molecular mechanisms remain incompletely elucidated.. Among tobacco-derived carcinogens, 4(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a key one, which has been demonstrated to enhance the malignant progression of CRC.. CUT&RUN-seq and mRNA-seq analyses, along with subsequent validation experiments, reveal that NNK upregulates the expression of the acetyltransferase p300.

Article Title: The role of R-loop aberrations in lower-grade gliomas: prognostic, immune, and metabolic implications from multi-omics and machine learning analysis
Article Snippet: Equal amounts of protein were mixed with loading buffer (Cat. No. P0015, Beyotime), boiled at 98 °C for 10 min, separated on 7.5% SDS-PAGE gels (Cat. No. PG111, Epizyme) and transferred to 0.22 μm polyvinylidene fluoride membranes (Millipore). .. Membranes were blocked with Fast Blocking Fluid (Cat. No. G2052, Servicebio) for 15 min at room temperature, incubated overnight at 4 °C with primary antibodies, washed with 0.1% TBST, and incubated with secondary antibodies for 1 h. Signals were detected using a Tanon imaging system and quantified with ImageJ. .. The primary antibodies were: anti-INCENP (Cat. No. 30388, ProMab), anti-BAX (Cat.No.

Article Title: Angelica sinensis polysaccharides modulate iron metabolism and macrophage polarization to alleviate rheumatoid arthritis.
Article Snippet: Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by joint inflammation and systemic complications.. Recent evidence has indicated that disrupted iron metabolism, notably elevated hepcidin expression and excessive iron accumulation in tissues, contributes to RA progression.. In RA, Macrophages, which play a central role in both immune regulation and iron homeostasis, are often polarized toward the proinflammatory M1 phenotype.

Article Title: YBX1 Confers immunosuppressive bone metastatic traits in non-small cell lung cancer.
Article Snippet: Subsequently, the sections were treated with antigen retrieval buffer (Servicebio, G1202) and 3% hydrogen peroxide at room temperature for 25 minutes protected from light. .. A blocking solution of 3% bovine serum albumin (Servicebio, GC305010) was applied and incubated at room temperature for 30 minutes. .. The sections were then incubated overnight at 4°C with the following primary antibodies, respectively, followed by three washes with PBS buffer (pH 7.4): FOXP3 (iF546-Tyramide, Proteintech, 65089-1-Ig, 1:500), CD4 (iF594-Tyramide, Proteintech, 86300-3-RR, 1:500), IFN-γ (iF647-Tyramide, Proteintech, 29788-1-AP, 1:500), YBX1 (iF700-Tyramide, Proteintech, 20339-1-AP, 1:400), CD8 (iF488Tyramide, Proteintech, 85977-4-RR, 1:500), and CD25 (iF440-Tyramide, CST, 39475S, 1:200).

Article Title: Dietary riboflavin blocks the cGAS-STING pathway to curb hypoxia-induced hepatic inflammation in sub-adult grass carp (Ctenopharyngodon idella)
Article Snippet: .. The membranes were blocked with blocking solution (G2052-210 Jo urn al Pr e-p roo f 500 ml, Servicebio Biotechnology Co., Ltd., Wuhan, Hubei, China) and then incubated 211 overnight at 4 °C with primary antibodies. .. Subsequently, the membranes were exposed 212 to the secondary antibody for 1.5 h. The bands were detected using an ECL kit (PD203-213 500 ml, Oriscience Biotechnology Co., Ltd., Chengdu, Sichuan, China), imaged using 214 the multimodal imaging system (ChemiDoc, Bio-Rad Laboratories, Inc., USA), and 215 analyzed with ImageJ software (1.42q).

Control:

Article Title: Acetyltransferase p300 Promotes NNK-Induced Colorectal Cancer Progression by Mediating ANKHD1 Expression via H3K27ac Modification.
Article Snippet: Cigarette smoking significantly accelerates the initiation and progression of colorectal cancer (CRC), although the precise molecular mechanisms remain incompletely elucidated.. Among tobacco-derived carcinogens, 4(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) is a key one, which has been demonstrated to enhance the malignant progression of CRC.. CUT&RUN-seq and mRNA-seq analyses, along with subsequent validation experiments, reveal that NNK upregulates the expression of the acetyltransferase p300.

Imaging:

Article Title: The role of R-loop aberrations in lower-grade gliomas: prognostic, immune, and metabolic implications from multi-omics and machine learning analysis
Article Snippet: Equal amounts of protein were mixed with loading buffer (Cat. No. P0015, Beyotime), boiled at 98 °C for 10 min, separated on 7.5% SDS-PAGE gels (Cat. No. PG111, Epizyme) and transferred to 0.22 μm polyvinylidene fluoride membranes (Millipore). .. Membranes were blocked with Fast Blocking Fluid (Cat. No. G2052, Servicebio) for 15 min at room temperature, incubated overnight at 4 °C with primary antibodies, washed with 0.1% TBST, and incubated with secondary antibodies for 1 h. Signals were detected using a Tanon imaging system and quantified with ImageJ. .. The primary antibodies were: anti-INCENP (Cat. No. 30388, ProMab), anti-BAX (Cat.No.



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