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Epizyme Inc non enzymatic rapid blocking solution
Non Enzymatic Rapid Blocking Solution, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rapid+blocking+solution/blocking+buffer+rapid/pm42161691-98-3-7
Average 86 stars, based on 1 article reviews
non enzymatic rapid blocking solution - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Blocking Assay:

Article Title: miR-9394b from Lactobacillus paracasei extracellular vesicles targets iNOS to reprogram host NO-ornithine metabolism and ameliorate DSS-induced murine colitis.
Article Snippet: .. The membranes were blocked with Rapid Blocking Buffer (Epizyme) for 15 min and then washed with Trisbuffered saline containing 0.1% Tween-20 (TBST). ..

Article Title: Aspartame may promote erectile dysfunction via DPP4-mediated endothelial dysfunction and apoptosis: evidence from network toxicology, molecular dynamics simulation, and experimental validation
Article Snippet: .. Proteins were then transferred onto 0.45 μm PVDF membranes at a constant current of 400 mA for 30 min. After transfer, the membranes were blocked with rapid blocking solution (Epizyme Biotech, China) for 15 min at room temperature and washed with TBST buffer. .. The PVDF membranes were then incubated overnight at 4 °C with the following primary antibodies: DPP4 (Proteintech, China, 1:1000), Bcl-2 (Proteintech, China, 1:5000), Bax (Proteintech, China, 1:5000), p-eNOS (Cell Signaling Technology, United States, 1:1000), eNOS (Cell Signaling Technology, United States, 1:1000) and β-actin (Proteintech, China, 1:10000).

Article Title: RNF138-Mediated Ubiquitination and Degradation of NS5 Restricts Tick-Borne Encephalitis Virus Infection.
Article Snippet: .. Proteins were transferred to PVDF membranes and blocked with blocking buffer (Epizyme Biotech, PS108, China) for 0.5 h at 24◦C. .. Membranes were then incubated overnight at 4◦C with primary antibodies, followed by incubation with HRP-conjugated secondary antibodies (Jackson ImmunoResearch, 115-035-062 [anti-mouse] and 111-035-045 [anti-rabbit], USA) for 1 h at 24◦C.

Article Title: Tunnelling Nanotube-Mediated Lysosome Sharing Promotes Osteocyte Survival via Transcellular Autophagy.
Article Snippet: .. The membranes were blocked with Rapid Blocking Buffer (PS108P, Epizyme Biotech, China) and incubated overnight at 4°C with rabbit anti- p62 (1:1000, A0964, ABclonal, China), rabbit anti- LC3B (1:1000, A19665, ABclonal, China) and mouse anti- β- actin (1:50000, 66009- 1- Ig, Proteintech, USA). .. After TBST washes, membranes were incubated with HRP- conjugated goat anti- rabbit IgG (1:10000, SA00001- 2, Proteintech, USA) or HRP- conjugated goat anti- mouse IgG (1:10000, SA00001- 1, Proteintech, USA) for 2 h. Protein bands were visualized by an enhanced chemiluminescence detection kit (PK10001, Proteintech, USA) and signals were captured using a multifunctional gel imaging analysis system (5200, Tanon, China).

Article Title: Exploring potential targets and mechanisms of male reproductive toxicity induced by the emerging PFAS GenX and F-53B via network toxicology, molecular docking, and in vivo validation.
Article Snippet: Protein samples were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes (1620177; Bio-Rad, Hercules, CA, USA). .. Membranes were blocked with Rapid Blocking Buffer (PS108P; Epizyme Biomedical Technology Co., Ltd., Shanghai, China) for 15 min, washed three times with tris-buffered saline with Tween-20 (TBST), and incubated with primary antibodies at 4°C overnight. .. Primary antibodies included anti-estrogen receptor alpha (ERα; ab32063; Abcam, Eugene, OR, USA), anti-c-Myc (ab32072; Abcam), anti-β-tubulin (A12289; ABclonal Technology Co., Ltd., Wuhan, China), anti-GAPDH (A19056; ABclonal Technology Co., Ltd.), and anti-TNF-α (A22227; ABclonal Technology Co., Ltd.).

Article Title: DHRS9 knockdown attenuates severe acute pancreatitis by inhibiting ferroptosis through the modulation of redox homeostasis and oxylipin metabolism.
Article Snippet: Equal amounts of protein were separated by SDS–PAGE and electroblotted onto PVDF membranes (Millipore). .. After blocking with blocking solution (Epizyme) at room temperature for 30 min, the membranes were incubated overnight with anti-DHRS9 (1:2000; Upingbio), anti-SLC7A11 (1:2000; Zenbio), anti-ACSL4 (1:3000; Proteintech), anti-GPX4 (1:2000; Proteintech), anti-NAMPT (1:2000; FineTest), anti-NMNAT3 (1:2000; FineTest), anti-NMNAT1 (1:2000; Proteintech), anti-NMNAT2 (1:2000; Proteintech) and anti-tubulin (1:5000; FineTest) antibodies at 4 ◦C. .. The membranes were subsequently washed three times with 1× TBST and incubated with HRPconjugated anti-rabbit or anti-mouse secondary antibodies (New Cell & Molecular Biotech) for 1 h at room temperature.

Article Title: Low-intensity Pulsed Ultrasound Ameliorates Obesity-induced Hepatic Lipid Metabolic Disorders.
Article Snippet: Objective: To investigate the therapeutic potential of low-intensity pulsed ultrasound (LIPUS) in ameliorating obesity-induced hepatic steatosis and exploring its underlying mechanisms.. Methods: Thirty male C57BL/6J mice were divided into normal diet (ND), high-fat diet (HFD), and HFD with LIPUS treated (HFD + LIPUS) groups.. The ND group was fed standard diet throughout the 12-wk experimental period.

Article Title: Physiological and metabolic orchestration during biphasic low-salinity adaptation in the mud crab (Scylla paramamosain): Insights into osmoregulation, lipid remodeling, and energy metabolism.
Article Snippet: As a euryhaline crustacean, Scylla paramamosain plays an important role in mariculture.. Investigating its salinity adaptation mechanisms is critical for coping with salinity fluctuations in coastal aquaculture and promoting the sustainable development of inland aquaculture models.. This study investigated the adaptive response of Scylla paramamosain by sampling crabs at 0, 1, 3, 6, 12, 24, 48, 72, 120, and 192 h after their abrupt transfer from normal salinity (NS, 25‰) to low salinity (LS, 6‰) seawater, and further at 6, 24, and 48 h after their transfer back from LS (at 192 h) back to NS.

Saline:

Article Title: miR-9394b from Lactobacillus paracasei extracellular vesicles targets iNOS to reprogram host NO-ornithine metabolism and ameliorate DSS-induced murine colitis.
Article Snippet: .. The membranes were blocked with Rapid Blocking Buffer (Epizyme) for 15 min and then washed with Trisbuffered saline containing 0.1% Tween-20 (TBST). ..

Article Title: Exploring potential targets and mechanisms of male reproductive toxicity induced by the emerging PFAS GenX and F-53B via network toxicology, molecular docking, and in vivo validation.
Article Snippet: Protein samples were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes (1620177; Bio-Rad, Hercules, CA, USA). .. Membranes were blocked with Rapid Blocking Buffer (PS108P; Epizyme Biomedical Technology Co., Ltd., Shanghai, China) for 15 min, washed three times with tris-buffered saline with Tween-20 (TBST), and incubated with primary antibodies at 4°C overnight. .. Primary antibodies included anti-estrogen receptor alpha (ERα; ab32063; Abcam, Eugene, OR, USA), anti-c-Myc (ab32072; Abcam), anti-β-tubulin (A12289; ABclonal Technology Co., Ltd., Wuhan, China), anti-GAPDH (A19056; ABclonal Technology Co., Ltd.), and anti-TNF-α (A22227; ABclonal Technology Co., Ltd.).

Incubation:

Article Title: Tunnelling Nanotube-Mediated Lysosome Sharing Promotes Osteocyte Survival via Transcellular Autophagy.
Article Snippet: .. The membranes were blocked with Rapid Blocking Buffer (PS108P, Epizyme Biotech, China) and incubated overnight at 4°C with rabbit anti- p62 (1:1000, A0964, ABclonal, China), rabbit anti- LC3B (1:1000, A19665, ABclonal, China) and mouse anti- β- actin (1:50000, 66009- 1- Ig, Proteintech, USA). .. After TBST washes, membranes were incubated with HRP- conjugated goat anti- rabbit IgG (1:10000, SA00001- 2, Proteintech, USA) or HRP- conjugated goat anti- mouse IgG (1:10000, SA00001- 1, Proteintech, USA) for 2 h. Protein bands were visualized by an enhanced chemiluminescence detection kit (PK10001, Proteintech, USA) and signals were captured using a multifunctional gel imaging analysis system (5200, Tanon, China).

Article Title: Exploring potential targets and mechanisms of male reproductive toxicity induced by the emerging PFAS GenX and F-53B via network toxicology, molecular docking, and in vivo validation.
Article Snippet: Protein samples were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred to polyvinylidene difluoride (PVDF) membranes (1620177; Bio-Rad, Hercules, CA, USA). .. Membranes were blocked with Rapid Blocking Buffer (PS108P; Epizyme Biomedical Technology Co., Ltd., Shanghai, China) for 15 min, washed three times with tris-buffered saline with Tween-20 (TBST), and incubated with primary antibodies at 4°C overnight. .. Primary antibodies included anti-estrogen receptor alpha (ERα; ab32063; Abcam, Eugene, OR, USA), anti-c-Myc (ab32072; Abcam), anti-β-tubulin (A12289; ABclonal Technology Co., Ltd., Wuhan, China), anti-GAPDH (A19056; ABclonal Technology Co., Ltd.), and anti-TNF-α (A22227; ABclonal Technology Co., Ltd.).

Article Title: DHRS9 knockdown attenuates severe acute pancreatitis by inhibiting ferroptosis through the modulation of redox homeostasis and oxylipin metabolism.
Article Snippet: Equal amounts of protein were separated by SDS–PAGE and electroblotted onto PVDF membranes (Millipore). .. After blocking with blocking solution (Epizyme) at room temperature for 30 min, the membranes were incubated overnight with anti-DHRS9 (1:2000; Upingbio), anti-SLC7A11 (1:2000; Zenbio), anti-ACSL4 (1:3000; Proteintech), anti-GPX4 (1:2000; Proteintech), anti-NAMPT (1:2000; FineTest), anti-NMNAT3 (1:2000; FineTest), anti-NMNAT1 (1:2000; Proteintech), anti-NMNAT2 (1:2000; Proteintech) and anti-tubulin (1:5000; FineTest) antibodies at 4 ◦C. .. The membranes were subsequently washed three times with 1× TBST and incubated with HRPconjugated anti-rabbit or anti-mouse secondary antibodies (New Cell & Molecular Biotech) for 1 h at room temperature.

Article Title: Low-intensity Pulsed Ultrasound Ameliorates Obesity-induced Hepatic Lipid Metabolic Disorders.
Article Snippet: Objective: To investigate the therapeutic potential of low-intensity pulsed ultrasound (LIPUS) in ameliorating obesity-induced hepatic steatosis and exploring its underlying mechanisms.. Methods: Thirty male C57BL/6J mice were divided into normal diet (ND), high-fat diet (HFD), and HFD with LIPUS treated (HFD + LIPUS) groups.. The ND group was fed standard diet throughout the 12-wk experimental period.

Membrane:

Article Title: Physiological and metabolic orchestration during biphasic low-salinity adaptation in the mud crab (Scylla paramamosain): Insights into osmoregulation, lipid remodeling, and energy metabolism.
Article Snippet: As a euryhaline crustacean, Scylla paramamosain plays an important role in mariculture.. Investigating its salinity adaptation mechanisms is critical for coping with salinity fluctuations in coastal aquaculture and promoting the sustainable development of inland aquaculture models.. This study investigated the adaptive response of Scylla paramamosain by sampling crabs at 0, 1, 3, 6, 12, 24, 48, 72, 120, and 192 h after their abrupt transfer from normal salinity (NS, 25‰) to low salinity (LS, 6‰) seawater, and further at 6, 24, and 48 h after their transfer back from LS (at 192 h) back to NS.

Gentle:

Article Title: Physiological and metabolic orchestration during biphasic low-salinity adaptation in the mud crab (Scylla paramamosain): Insights into osmoregulation, lipid remodeling, and energy metabolism.
Article Snippet: As a euryhaline crustacean, Scylla paramamosain plays an important role in mariculture.. Investigating its salinity adaptation mechanisms is critical for coping with salinity fluctuations in coastal aquaculture and promoting the sustainable development of inland aquaculture models.. This study investigated the adaptive response of Scylla paramamosain by sampling crabs at 0, 1, 3, 6, 12, 24, 48, 72, 120, and 192 h after their abrupt transfer from normal salinity (NS, 25‰) to low salinity (LS, 6‰) seawater, and further at 6, 24, and 48 h after their transfer back from LS (at 192 h) back to NS.



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