random hexanucleotide primers (Millipore)
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Millipore
random hexanucleotide primers
Random Hexanucleotide Primers, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+primer+hexanucleotides/random+hexamer+primers/pm37371461-64-43-46
Average 90 stars, based on 1 article reviews
Random Hexanucleotide Primers, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/random+primer+hexanucleotides/random+hexamer+primers/pm37371461-64-43-46
Average 90 stars, based on 1 article reviews
random hexanucleotide primers - by Bioz Stars,
2026-10
90/100 stars
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Extraction:Article Title: Nucleolar Localization of the RNA Helicase DDX21 Predicts Survival Outcomes in Gynecologic Cancers Article Snippet: The membranes were washed extensively with TBS-T before chemiluminescent detection using SuperSignal West Pico substrate (Thermo Fisher Scientific) and ChemiDoc system (Bio-Rad). .. cDNA pools were prepared by extraction of nuclear RNA from cells treated with niraparib using the Qiagen RNeasy Plus Mini Kit, followed by reverse transcription using MMLV reverse transcriptase (Progmega, M150B) with random Reverse Transcription:Article Title: Nucleolar Localization of the RNA Helicase DDX21 Predicts Survival Outcomes in Gynecologic Cancers Article Snippet: The membranes were washed extensively with TBS-T before chemiluminescent detection using SuperSignal West Pico substrate (Thermo Fisher Scientific) and ChemiDoc system (Bio-Rad). .. cDNA pools were prepared by extraction of nuclear RNA from cells treated with niraparib using the Qiagen RNeasy Plus Mini Kit, followed by reverse transcription using MMLV reverse transcriptase (Progmega, M150B) with random Article Title: Systematic surveillance of SARS-CoV-2 reveals dynamics of variant mutagenesis and transmission in a large urban population Article Snippet: .. Total RNA was reverse transcribed using SuperScriptTM IV Reverse Transcriptase (Invitrogen) in 5X First-Strand Buffer containing DTT, a custom mix of Oligo-dT (Sigma), and Article Title: A noncanonical repressor function of JUN restrains YAP activity and suppresses YAP-dependent liver cancer growth Article Snippet: Total RNA was extracted with peqGOLD TriFast Reagent (VWR). .. First-strand cDNA synthesis was performed from 1 μg RNA using M-MLV Reverse Transcriptase (Promega) and Article Title: HIV-1 Transcriptional Activator Tat Inhibits IL2 Expression by Preventing the Presence of Pol II on the IL2 Promoter. Article Snippet: RNA yield and purity were determined by measuring absorbance at 260/280 nm on a Quawell microvolume spectrophotometer Q3000 (Quawell Technology). .. Total RNA (100 ng per reaction) was used for cDNA synthesis using M-MLV reverse transcriptase (200 U/μL) (Sigma-Aldrich, St. Louis, MI, USA) in 10× M-MLV reverse transcriptase buffer, 40 U/μL RNase inhibitor (Thermo Fisher Scientific-Invitrogen), 1 mM each dNTPs (Sigma-Aldrich), and 2.5 μM Article Title: Genome-wide analysis of growth phase-dependent translational and transcriptional regulation in halophilic archaea Article Snippet: .. Reverse transcription of 2 μg RNA was carried out with 400 U M-MLV reverse transcriptase RNase H minus (Promega, Mannheim, Germany) and 0.6 μg Article Title: Gypenoside A Protects Human Myocardial Cells from Ischemia/Reperfusion Injury via the circ_0010729/miR-370-3p/RUNX1 Axis Article Snippet: Ischemia/reperfusion (I/R) injury is one of the major causes of cardiovascular disease.. Gypenoside A (GP), the main active component of Gynostemma pentaphyllum, alleviates myocardial I/R injury.. Circular RNAs (circRNAs) and microRNAs (miRNAs) are involved in the I/R injury. Random Hexamer:Article Title: Nucleolar Localization of the RNA Helicase DDX21 Predicts Survival Outcomes in Gynecologic Cancers Article Snippet: The membranes were washed extensively with TBS-T before chemiluminescent detection using SuperSignal West Pico substrate (Thermo Fisher Scientific) and ChemiDoc system (Bio-Rad). .. cDNA pools were prepared by extraction of nuclear RNA from cells treated with niraparib using the Qiagen RNeasy Plus Mini Kit, followed by reverse transcription using MMLV reverse transcriptase (Progmega, M150B) with random Article Title: Prevalence of HSPB6 gene variants in peripartum cardiomyopathy: Data from the German PPCM registry. Article Snippet: The in silico splicing tools SSF, MaxEntScan and NNSPLICE (integrated part of Alamut v2.11.0, Interactive Biosoftware, Rouen, France) were used for splicing prediction. .. Total RNA from myocardial biopsies were isolated with TRIzol® Reagent (Life technologies) in accordance with the manufacturer’s instructions. cDNA synthesis using Superscript III (Invitrogen), 2 μg of total RNA and Article Title: A noncanonical repressor function of JUN restrains YAP activity and suppresses YAP-dependent liver cancer growth Article Snippet: Total RNA was extracted with peqGOLD TriFast Reagent (VWR). .. First-strand cDNA synthesis was performed from 1 μg RNA using M-MLV Reverse Transcriptase (Promega) and Article Title: Genome-wide analysis of growth phase-dependent translational and transcriptional regulation in halophilic archaea Article Snippet: .. Reverse transcription of 2 μg RNA was carried out with 400 U M-MLV reverse transcriptase RNase H minus (Promega, Mannheim, Germany) and 0.6 μg Article Title: Gypenoside A Protects Human Myocardial Cells from Ischemia/Reperfusion Injury via the circ_0010729/miR-370-3p/RUNX1 Axis Article Snippet: Ischemia/reperfusion (I/R) injury is one of the major causes of cardiovascular disease.. Gypenoside A (GP), the main active component of Gynostemma pentaphyllum, alleviates myocardial I/R injury.. Circular RNAs (circRNAs) and microRNAs (miRNAs) are involved in the I/R injury. Isolation:Article Title: Prevalence of HSPB6 gene variants in peripartum cardiomyopathy: Data from the German PPCM registry. Article Snippet: The in silico splicing tools SSF, MaxEntScan and NNSPLICE (integrated part of Alamut v2.11.0, Interactive Biosoftware, Rouen, France) were used for splicing prediction. .. Total RNA from myocardial biopsies were isolated with TRIzol® Reagent (Life technologies) in accordance with the manufacturer’s instructions. cDNA synthesis using Superscript III (Invitrogen), 2 μg of total RNA and cDNA Synthesis:Article Title: Prevalence of HSPB6 gene variants in peripartum cardiomyopathy: Data from the German PPCM registry. Article Snippet: The in silico splicing tools SSF, MaxEntScan and NNSPLICE (integrated part of Alamut v2.11.0, Interactive Biosoftware, Rouen, France) were used for splicing prediction. .. Total RNA from myocardial biopsies were isolated with TRIzol® Reagent (Life technologies) in accordance with the manufacturer’s instructions. cDNA synthesis using Superscript III (Invitrogen), 2 μg of total RNA and Article Title: A noncanonical repressor function of JUN restrains YAP activity and suppresses YAP-dependent liver cancer growth Article Snippet: Total RNA was extracted with peqGOLD TriFast Reagent (VWR). .. First-strand cDNA synthesis was performed from 1 μg RNA using M-MLV Reverse Transcriptase (Promega) and Article Title: HIV-1 Transcriptional Activator Tat Inhibits IL2 Expression by Preventing the Presence of Pol II on the IL2 Promoter. Article Snippet: RNA yield and purity were determined by measuring absorbance at 260/280 nm on a Quawell microvolume spectrophotometer Q3000 (Quawell Technology). .. Total RNA (100 ng per reaction) was used for cDNA synthesis using M-MLV reverse transcriptase (200 U/μL) (Sigma-Aldrich, St. Louis, MI, USA) in 10× M-MLV reverse transcriptase buffer, 40 U/μL RNase inhibitor (Thermo Fisher Scientific-Invitrogen), 1 mM each dNTPs (Sigma-Aldrich), and 2.5 μM Real-time Polymerase Chain Reaction:Article Title: A noncanonical repressor function of JUN restrains YAP activity and suppresses YAP-dependent liver cancer growth Article Snippet: Total RNA was extracted with peqGOLD TriFast Reagent (VWR). .. First-strand cDNA synthesis was performed from 1 μg RNA using M-MLV Reverse Transcriptase (Promega) and Article Title: Unique features of KGN granulosa-like tumour cells in the regulation of steroidogenic and antioxidant genes. Article Snippet: DNaseI (Promega/Life Technologies Australia Pty Ltd, Tullamarine, Vic, Australia) was also employed to eliminate the potential genomic DNA contamination of the RNA. .. One hundred ng of high-quality DNase-treated RNA was used to synthesise cDNA with 250 ng Article Title: HIV-1 Transcriptional Activator Tat Inhibits IL2 Expression by Preventing the Presence of Pol II on the IL2 Promoter. Article Snippet: RNA yield and purity were determined by measuring absorbance at 260/280 nm on a Quawell microvolume spectrophotometer Q3000 (Quawell Technology). .. Total RNA (100 ng per reaction) was used for cDNA synthesis using M-MLV reverse transcriptase (200 U/μL) (Sigma-Aldrich, St. Louis, MI, USA) in 10× M-MLV reverse transcriptase buffer, 40 U/μL RNase inhibitor (Thermo Fisher Scientific-Invitrogen), 1 mM each dNTPs (Sigma-Aldrich), and 2.5 μM Polymerase Chain Reaction:Article Title: Unique features of KGN granulosa-like tumour cells in the regulation of steroidogenic and antioxidant genes. Article Snippet: DNaseI (Promega/Life Technologies Australia Pty Ltd, Tullamarine, Vic, Australia) was also employed to eliminate the potential genomic DNA contamination of the RNA. .. One hundred ng of high-quality DNase-treated RNA was used to synthesise cDNA with 250 ng Fluorescence:Article Title: HIV-1 Transcriptional Activator Tat Inhibits IL2 Expression by Preventing the Presence of Pol II on the IL2 Promoter. Article Snippet: RNA yield and purity were determined by measuring absorbance at 260/280 nm on a Quawell microvolume spectrophotometer Q3000 (Quawell Technology). .. Total RNA (100 ng per reaction) was used for cDNA synthesis using M-MLV reverse transcriptase (200 U/μL) (Sigma-Aldrich, St. Louis, MI, USA) in 10× M-MLV reverse transcriptase buffer, 40 U/μL RNase inhibitor (Thermo Fisher Scientific-Invitrogen), 1 mM each dNTPs (Sigma-Aldrich), and 2.5 μM Purification:Article Title: Gypenoside A Protects Human Myocardial Cells from Ischemia/Reperfusion Injury via the circ_0010729/miR-370-3p/RUNX1 Axis Article Snippet: Ischemia/reperfusion (I/R) injury is one of the major causes of cardiovascular disease.. Gypenoside A (GP), the main active component of Gynostemma pentaphyllum, alleviates myocardial I/R injury.. Circular RNAs (circRNAs) and microRNAs (miRNAs) are involved in the I/R injury. |