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random hexanucleotide primers  (Thermo Fisher)


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    Structured Review

    Thermo Fisher random hexanucleotide primers
    Random Hexanucleotide Primers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/random+primer+hexanucleotides/pm37240031-344-21-24
    Average 90 stars, based on 1 article reviews
    random hexanucleotide primers - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: RNA Binding Protein CUGBP2/CELF2 Mediates Curcumin-Induced Mitotic Catastrophe of Pancreatic Cancer Cells
    Article Snippet: Results were analyzed with ModFit LT TM software (Verity Software House, Topsham, ME). .. Total RNA isolated from MiaPaCa-2, Pan02 cells and tumor xenografts using TRIZOL reagent was reverse transcribed with Superscript II reverse transcriptase in the presence of random hexanucleotide primers (all from Invitrogen, Carlsbad, CA). .. Complementary DNAs were then used for Real time PCR using Jumpstart Taq DNA polymerase (Sigma Chemical Co, St. Louis, MO) and SYBR Green nucleic acid stain (Molecular Probes, Eugene, OR).

    Reverse Transcription:

    Article Title: RNA Binding Protein CUGBP2/CELF2 Mediates Curcumin-Induced Mitotic Catastrophe of Pancreatic Cancer Cells
    Article Snippet: Results were analyzed with ModFit LT TM software (Verity Software House, Topsham, ME). .. Total RNA isolated from MiaPaCa-2, Pan02 cells and tumor xenografts using TRIZOL reagent was reverse transcribed with Superscript II reverse transcriptase in the presence of random hexanucleotide primers (all from Invitrogen, Carlsbad, CA). .. Complementary DNAs were then used for Real time PCR using Jumpstart Taq DNA polymerase (Sigma Chemical Co, St. Louis, MO) and SYBR Green nucleic acid stain (Molecular Probes, Eugene, OR).

    Article Title: Co-Surveillance of Rotaviruses in Humans and Domestic Animals in Central Uganda Reveals Circulation of Wide Genotype Diversity in the Animals.
    Article Snippet: .. The RNA was transcribed to cDNA using M-MLV reverse transcriptase and random hexanucleotide primers (Invitrogen, Life Technologies, Carlsbad, CA, USA) [31], and the resulting cDNA was used in the NSP3 real time RT-PCR and the subsequent VP7 and VP4 PCRs [28,31]. ..

    Article Title: Lipocalin-2 Deficiency Diminishes Canonical NLRP3 Inflammasome Formation and IL-1β Production in the Subacute Phase of Spinal Cord Injury.
    Article Snippet: Afterwards, purity and RNA concentration were measured using a NanoDrop 1000 device (PeqLab). .. For cDNA synthesis, 1 μg of RNA was used, and reverse transcription was performed using an MMLV reverse transcriptase kit and random hexanucleotide primers (Invitrogen, Braunschweig, Germany). .. Semiquantitative real-time PCR (qRT-PCR) was performed using SYBR Green (Bioline, Braine-Álleud, Belgium, QT615-05) in the CFX Connect qPCR detection system (Bio-Rad, Feldkirchen, Germany).

    Article Title: Investigations on SARS-CoV-2 and other coronaviruses in mink farms in France at the end of the first year of COVID-19 pandemic
    Article Snippet: .. The cDNA was synthesized by reverse transcription of 5μL of RNA extracted from tracheal swabs and faecal samples using 0.25μL of hexanucleotide primers (0.2μG/μL) (Thermo Fisher Scientific, Dardilly, France) and a RT Maxima H Minus cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific, Dardilly, France) according to the manufacturer’s instructions. ..

    Article Title: Co-Surveillance of Rotaviruses in Humans and Domestic Animals in Central Uganda Reveals Circulation of Wide Genotype Diversity in the Animals
    Article Snippet: .. The RNA was transcribed to cDNA using M-MLV reverse transcriptase and random hexanucleotide primers (Invitrogen, Life Technologies, Carlsbad, CA, USA) [ ], and the resulting cDNA was used in the NSP3 real time RT-PCR and the subsequent VP7 and VP4 PCRs [ , ]. ..

    Article Title: Isolation and phylogenetic characterization of arboviruses circulating among phlebotomine sandflies in parts of North Rift, Kenya
    Article Snippet: Library preparation, pooling, and highthroughput sequencing Library preparation for the CPE-positive samples was performed using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s instruction with slight modifications to exclude purification of the PolyA-containing Frontiers in Virology 04 mRNA procedure. .. Briefly, the RNA was fragmented and primed for cDNA synthesis using Superscript III reverse transcriptase (Invitrogen, Thermofisher Scientific, Waltham, MA, USA) and hexanucleotide primers (Invitrogen, Waltham, MA, USA). .. Second-strand cDNA synthesis was then performed using DNA Polymerase I and RNase H (Illumina, San Diego, CA, USA).

    Quantitative RT-PCR:

    Article Title: Co-Surveillance of Rotaviruses in Humans and Domestic Animals in Central Uganda Reveals Circulation of Wide Genotype Diversity in the Animals.
    Article Snippet: .. The RNA was transcribed to cDNA using M-MLV reverse transcriptase and random hexanucleotide primers (Invitrogen, Life Technologies, Carlsbad, CA, USA) [31], and the resulting cDNA was used in the NSP3 real time RT-PCR and the subsequent VP7 and VP4 PCRs [28,31]. ..

    Article Title: Co-Surveillance of Rotaviruses in Humans and Domestic Animals in Central Uganda Reveals Circulation of Wide Genotype Diversity in the Animals
    Article Snippet: .. The RNA was transcribed to cDNA using M-MLV reverse transcriptase and random hexanucleotide primers (Invitrogen, Life Technologies, Carlsbad, CA, USA) [ ], and the resulting cDNA was used in the NSP3 real time RT-PCR and the subsequent VP7 and VP4 PCRs [ , ]. ..

    cDNA Synthesis:

    Article Title: Lipocalin-2 Deficiency Diminishes Canonical NLRP3 Inflammasome Formation and IL-1β Production in the Subacute Phase of Spinal Cord Injury.
    Article Snippet: Afterwards, purity and RNA concentration were measured using a NanoDrop 1000 device (PeqLab). .. For cDNA synthesis, 1 μg of RNA was used, and reverse transcription was performed using an MMLV reverse transcriptase kit and random hexanucleotide primers (Invitrogen, Braunschweig, Germany). .. Semiquantitative real-time PCR (qRT-PCR) was performed using SYBR Green (Bioline, Braine-Álleud, Belgium, QT615-05) in the CFX Connect qPCR detection system (Bio-Rad, Feldkirchen, Germany).

    Article Title: Low sulfated heparan sulfate mimetic differentially affects repair in immune-mediated and toxin-induced experimental models of demyelination.
    Article Snippet: .. Total RNA was extracted using peqGOLD TriFast (VWR), and RNA concentration and purity were measured with the NanoDrop 1000 device (Thermo Scientific). cDNA synthesis was performed using moloney murine leukemia virus reserve transcriptase kit and random hexanucleotide primers (Thermo Fisher Scientific). cDNA levels were then analyzed by RT-rtPCR using AceQ® qPCR SYBR Green Master Mix (Vazyme). ..

    Article Title: Investigations on SARS-CoV-2 and other coronaviruses in mink farms in France at the end of the first year of COVID-19 pandemic
    Article Snippet: .. The cDNA was synthesized by reverse transcription of 5μL of RNA extracted from tracheal swabs and faecal samples using 0.25μL of hexanucleotide primers (0.2μG/μL) (Thermo Fisher Scientific, Dardilly, France) and a RT Maxima H Minus cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific, Dardilly, France) according to the manufacturer’s instructions. ..

    Article Title: Low sulfated heparan sulfate mimetic differentially affects repair in immune‐mediated and toxin‐induced experimental models of demyelination
    Article Snippet: .. Total RNA was extracted using peqGOLD TriFast (VWR), and RNA concentration and purity were measured with the NanoDrop 1000 device (Thermo Scientific). cDNA synthesis was performed using moloney murine leukemia virus reserve transcriptase kit and random hexanucleotide primers (Thermo Fisher Scientific). cDNA levels were then analyzed by RT‐rtPCR using AceQ® qPCR SYBR Green Master Mix (Vazyme). ..

    Article Title: Isolation and phylogenetic characterization of arboviruses circulating among phlebotomine sandflies in parts of North Rift, Kenya
    Article Snippet: Library preparation, pooling, and highthroughput sequencing Library preparation for the CPE-positive samples was performed using the TruSeq Stranded mRNA Library Prep Kit (Illumina, San Diego, CA, USA), according to the manufacturer’s instruction with slight modifications to exclude purification of the PolyA-containing Frontiers in Virology 04 mRNA procedure. .. Briefly, the RNA was fragmented and primed for cDNA synthesis using Superscript III reverse transcriptase (Invitrogen, Thermofisher Scientific, Waltham, MA, USA) and hexanucleotide primers (Invitrogen, Waltham, MA, USA). .. Second-strand cDNA synthesis was then performed using DNA Polymerase I and RNase H (Illumina, San Diego, CA, USA).

    Concentration Assay:

    Article Title: Low sulfated heparan sulfate mimetic differentially affects repair in immune-mediated and toxin-induced experimental models of demyelination.
    Article Snippet: .. Total RNA was extracted using peqGOLD TriFast (VWR), and RNA concentration and purity were measured with the NanoDrop 1000 device (Thermo Scientific). cDNA synthesis was performed using moloney murine leukemia virus reserve transcriptase kit and random hexanucleotide primers (Thermo Fisher Scientific). cDNA levels were then analyzed by RT-rtPCR using AceQ® qPCR SYBR Green Master Mix (Vazyme). ..

    Article Title: Low sulfated heparan sulfate mimetic differentially affects repair in immune‐mediated and toxin‐induced experimental models of demyelination
    Article Snippet: .. Total RNA was extracted using peqGOLD TriFast (VWR), and RNA concentration and purity were measured with the NanoDrop 1000 device (Thermo Scientific). cDNA synthesis was performed using moloney murine leukemia virus reserve transcriptase kit and random hexanucleotide primers (Thermo Fisher Scientific). cDNA levels were then analyzed by RT‐rtPCR using AceQ® qPCR SYBR Green Master Mix (Vazyme). ..

    Virus:

    Article Title: Low sulfated heparan sulfate mimetic differentially affects repair in immune-mediated and toxin-induced experimental models of demyelination.
    Article Snippet: .. Total RNA was extracted using peqGOLD TriFast (VWR), and RNA concentration and purity were measured with the NanoDrop 1000 device (Thermo Scientific). cDNA synthesis was performed using moloney murine leukemia virus reserve transcriptase kit and random hexanucleotide primers (Thermo Fisher Scientific). cDNA levels were then analyzed by RT-rtPCR using AceQ® qPCR SYBR Green Master Mix (Vazyme). ..

    Article Title: Low sulfated heparan sulfate mimetic differentially affects repair in immune‐mediated and toxin‐induced experimental models of demyelination
    Article Snippet: .. Total RNA was extracted using peqGOLD TriFast (VWR), and RNA concentration and purity were measured with the NanoDrop 1000 device (Thermo Scientific). cDNA synthesis was performed using moloney murine leukemia virus reserve transcriptase kit and random hexanucleotide primers (Thermo Fisher Scientific). cDNA levels were then analyzed by RT‐rtPCR using AceQ® qPCR SYBR Green Master Mix (Vazyme). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Low sulfated heparan sulfate mimetic differentially affects repair in immune-mediated and toxin-induced experimental models of demyelination.
    Article Snippet: .. Total RNA was extracted using peqGOLD TriFast (VWR), and RNA concentration and purity were measured with the NanoDrop 1000 device (Thermo Scientific). cDNA synthesis was performed using moloney murine leukemia virus reserve transcriptase kit and random hexanucleotide primers (Thermo Fisher Scientific). cDNA levels were then analyzed by RT-rtPCR using AceQ® qPCR SYBR Green Master Mix (Vazyme). ..

    Article Title: Low sulfated heparan sulfate mimetic differentially affects repair in immune‐mediated and toxin‐induced experimental models of demyelination
    Article Snippet: .. Total RNA was extracted using peqGOLD TriFast (VWR), and RNA concentration and purity were measured with the NanoDrop 1000 device (Thermo Scientific). cDNA synthesis was performed using moloney murine leukemia virus reserve transcriptase kit and random hexanucleotide primers (Thermo Fisher Scientific). cDNA levels were then analyzed by RT‐rtPCR using AceQ® qPCR SYBR Green Master Mix (Vazyme). ..

    SYBR Green Assay:

    Article Title: Low sulfated heparan sulfate mimetic differentially affects repair in immune-mediated and toxin-induced experimental models of demyelination.
    Article Snippet: .. Total RNA was extracted using peqGOLD TriFast (VWR), and RNA concentration and purity were measured with the NanoDrop 1000 device (Thermo Scientific). cDNA synthesis was performed using moloney murine leukemia virus reserve transcriptase kit and random hexanucleotide primers (Thermo Fisher Scientific). cDNA levels were then analyzed by RT-rtPCR using AceQ® qPCR SYBR Green Master Mix (Vazyme). ..

    Article Title: Low sulfated heparan sulfate mimetic differentially affects repair in immune‐mediated and toxin‐induced experimental models of demyelination
    Article Snippet: .. Total RNA was extracted using peqGOLD TriFast (VWR), and RNA concentration and purity were measured with the NanoDrop 1000 device (Thermo Scientific). cDNA synthesis was performed using moloney murine leukemia virus reserve transcriptase kit and random hexanucleotide primers (Thermo Fisher Scientific). cDNA levels were then analyzed by RT‐rtPCR using AceQ® qPCR SYBR Green Master Mix (Vazyme). ..

    Synthesized:

    Article Title: Investigations on SARS-CoV-2 and other coronaviruses in mink farms in France at the end of the first year of COVID-19 pandemic
    Article Snippet: .. The cDNA was synthesized by reverse transcription of 5μL of RNA extracted from tracheal swabs and faecal samples using 0.25μL of hexanucleotide primers (0.2μG/μL) (Thermo Fisher Scientific, Dardilly, France) and a RT Maxima H Minus cDNA synthesis kit (Invitrogen, Thermo Fisher Scientific, Dardilly, France) according to the manufacturer’s instructions. ..



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