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r045a mouse il  (TaKaRa)


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    Structured Review

    TaKaRa r045a mouse il
    R045a Mouse Il, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 5195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r045a+mouse+il/PrimeSTAR+Max+DNA+Polymerase/pm41557504-214-67-92
    Average 99 stars, based on 5195 article reviews
    r045a mouse il - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: The structural dynamics and molecular coupling in the slow inactivation of a prokaryotic voltage-gated sodium channel
    Article Snippet: The NavAb mutated DNAs were subcloned into the modified pBiEX-1 vector (Novagen) that wasmodified by replacing the fragment from NcoI site (5′-CCATGG-3′) to SalI site (5′-GTCGAC-3′) in the multicloning site with the sequence 5′-CCA TGG GCA GCA GCC ATC ATC ATC ATC ATC ACA GCA GCG GCC TGG TGC CGC GCG GCA GCC ATA TGC TCG AGC TGG TGC CGC GCG GCA GCG GAT CCT AAG TCG AC3′42,43. .. The polymerase chain reaction was used to perform sitedirected mutagenesis with PrimeSTAR Max DNA Polymerase (Takara Bio). ..

    Article Title: Citronellol Potentiates Antibiotics Against Escherichia coli O157:H7 via Inhibition of Biofilm Formation
    Article Snippet: .. PCR was performed with PrimeSTAR Max DNA Polymerase (Takara Bio Inc., Otsu, Shiga, Japan; Cat. No. R045A) in 2× Premix buffer following the manufacturer’s protocol, with initial denaturation at 98 ◦C for 3 min, 30 cycles of 98 ◦C for 10 s, 55 ◦C for 15 s, and 72 ◦C for 30 s. The eGFP fragment was amplified from a plasmid containing the eGFP gene using primers eGFP-F and eGFP-R (Supplementary Table S1). ..

    Article Title: Reprogramming the Rossmann fold signature motif creates orthogonal redox biocatalysts.
    Article Snippet: .. Fragments were obtained by PCR with PrimeSTAR Max DNA Polymerase (TaKaRa) and assembled using the Gibson isothermal DNA assemblymethod71. ..

    Article Title: A Ca 2 ⁺-responsive GH37 neutral trehalase from Lentinula edodes: Cloning, heterologous expression and biochemical characterization.
    Article Snippet: Trehalases (EC 3.2.1.28) hydrolyze trehalose into two glucose molecules and play important roles in fungal trehalose metabolism.. Although GH37 neutral trehalases have been characterized in yeasts and filamentous fungi, their biochemical properties in basidiomycetes remain poorly understood.. In this study, we cloned LeNTH1, a putative GH37 trehalase gene from Lentinula edodes, expressed it in Pichia pastoris, and characterized the purified recombinant enzyme.

    Mutagenesis:

    Article Title: The structural dynamics and molecular coupling in the slow inactivation of a prokaryotic voltage-gated sodium channel
    Article Snippet: The NavAb mutated DNAs were subcloned into the modified pBiEX-1 vector (Novagen) that wasmodified by replacing the fragment from NcoI site (5′-CCATGG-3′) to SalI site (5′-GTCGAC-3′) in the multicloning site with the sequence 5′-CCA TGG GCA GCA GCC ATC ATC ATC ATC ATC ACA GCA GCG GCC TGG TGC CGC GCG GCA GCC ATA TGC TCG AGC TGG TGC CGC GCG GCA GCG GAT CCT AAG TCG AC3′42,43. .. The polymerase chain reaction was used to perform sitedirected mutagenesis with PrimeSTAR Max DNA Polymerase (Takara Bio). ..

    Amplification:

    Article Title: Haematopoietic Cells Deficient for Calreticulin or Expressing Its Oncogenic Variants Maintain Functional Antigen Presentation
    Article Snippet: .. Primers were ordered from Sigma–Aldrich; fragments were amplified by PrimeSTAR Max DNA Polymerase (Takara), assembled by NEBuilder HiFi DNA Assembly Master Mix (NEB), and constructs were validated by Sanger sequencing. ..

    Article Title: Citronellol Potentiates Antibiotics Against Escherichia coli O157:H7 via Inhibition of Biofilm Formation
    Article Snippet: .. PCR was performed with PrimeSTAR Max DNA Polymerase (Takara Bio Inc., Otsu, Shiga, Japan; Cat. No. R045A) in 2× Premix buffer following the manufacturer’s protocol, with initial denaturation at 98 ◦C for 3 min, 30 cycles of 98 ◦C for 10 s, 55 ◦C for 15 s, and 72 ◦C for 30 s. The eGFP fragment was amplified from a plasmid containing the eGFP gene using primers eGFP-F and eGFP-R (Supplementary Table S1). ..

    Construct:

    Article Title: Haematopoietic Cells Deficient for Calreticulin or Expressing Its Oncogenic Variants Maintain Functional Antigen Presentation
    Article Snippet: .. Primers were ordered from Sigma–Aldrich; fragments were amplified by PrimeSTAR Max DNA Polymerase (Takara), assembled by NEBuilder HiFi DNA Assembly Master Mix (NEB), and constructs were validated by Sanger sequencing. ..

    DNA Sequencing:

    Article Title: Haematopoietic Cells Deficient for Calreticulin or Expressing Its Oncogenic Variants Maintain Functional Antigen Presentation
    Article Snippet: .. Primers were ordered from Sigma–Aldrich; fragments were amplified by PrimeSTAR Max DNA Polymerase (Takara), assembled by NEBuilder HiFi DNA Assembly Master Mix (NEB), and constructs were validated by Sanger sequencing. ..

    Cloning:

    Article Title: Efficient production of recombinant human serum albumin in Escherichia coli via oxidative folding mediated by the a domain of protein disulfide isomerase.
    Article Snippet: Efficient production of properly folded, disulfide-rich proteins in the cytosol of Escherichia coli remains challenging because the cytosol is highly reducing.. Here, we developed an expression system for recombinant human serum albumin (rHSA) by fusing the a domain of human protein disulfide isomerase (PDI) to the N-terminus of HSA.. PDI(a)mediated cis-fusion enabled high-level production of soluble rHSA in oxidizing E. coli strains under low-temperature conditions.

    Plasmid Preparation:

    Article Title: Citronellol Potentiates Antibiotics Against Escherichia coli O157:H7 via Inhibition of Biofilm Formation
    Article Snippet: .. PCR was performed with PrimeSTAR Max DNA Polymerase (Takara Bio Inc., Otsu, Shiga, Japan; Cat. No. R045A) in 2× Premix buffer following the manufacturer’s protocol, with initial denaturation at 98 ◦C for 3 min, 30 cycles of 98 ◦C for 10 s, 55 ◦C for 15 s, and 72 ◦C for 30 s. The eGFP fragment was amplified from a plasmid containing the eGFP gene using primers eGFP-F and eGFP-R (Supplementary Table S1). ..

    Sequencing:

    Article Title: A Ca 2 ⁺-responsive GH37 neutral trehalase from Lentinula edodes: Cloning, heterologous expression and biochemical characterization.
    Article Snippet: Trehalases (EC 3.2.1.28) hydrolyze trehalose into two glucose molecules and play important roles in fungal trehalose metabolism.. Although GH37 neutral trehalases have been characterized in yeasts and filamentous fungi, their biochemical properties in basidiomycetes remain poorly understood.. In this study, we cloned LeNTH1, a putative GH37 trehalase gene from Lentinula edodes, expressed it in Pichia pastoris, and characterized the purified recombinant enzyme.



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    99
    TaKaRa r045a mouse il
    R045a Mouse Il, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/r045a+mouse+il/PrimeSTAR+Max+DNA+Polymerase/pm41557504-214-67-92
    Average 99 stars, based on 1 article reviews
    r045a mouse il - by Bioz Stars, 2026-10
    99/100 stars
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