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quickblocktm blocking buffer for immunol staining (beyotime, p0260)  (Beyotime)


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    Beyotime quickblocktm blocking buffer for immunol staining (beyotime, p0260)
    Quickblocktm Blocking Buffer For Immunol Staining (Beyotime, P0260), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quickblocktm+buffer+p0260/quickblocktm+blocking+buffer/pm40319251-104-3-7
    Average 90 stars, based on 1 article reviews
    quickblocktm blocking buffer for immunol staining (beyotime, p0260) - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Exosomal miR-30a-5p promoted intrahepatic cholangiocarcinoma progression by increasing angiogenesis and vascular permeability dependent on PDCD10
    Article Snippet: .. Then, the gel was transferred to nitrocellulose membranes, blocked with QuickBlockTM Blocking Buffer (Beyotime, China) for 15 minutes, incubated with primary antibody overnight at 4◦C and with secondary antibody at room temperature for 2h. .. The exposed Proteins images were visualized with ECL Plus (EMD Millipore, Billarica, MA, USA).

    Article Title: Milk-derived exosomes exert anti-inflammatory activity in lipopolysaccharide-induced RAW264.7 cells by modulating the TLR4/NF-κB and PI3K/AKT signaling pathways
    Article Snippet: Proteins were subsequently transferred from the gel to a PVDF membrane (MilliporeSigma). .. The PVDF membrane was treated with a blocking solution (QuickBlockTM Western; cat. no. P0252; Beyotime Institute of Biotechnology) to inhibit non-specific binding at room temperature for 10 min, then the membranes were incubated at 4 ̊C for 12-16 h with the primary antibodies. ..

    Article Title: Noninvasive method for achieving the regeneration of damaged nerves via ultrasonic nasal drops.
    Article Snippet: .. The membrane was first blocked with QuickBlockTM Blocking Buffer (Beyotime, China) for 30 min, followed by overnight incubation at 4 ◦C with primary antibodies. .. After being washed three times with Western Wash Buffer (Beyotime, China), the membrane was incubated with a secondary antibody—either goat antimouse (1:400, Beyotime, China) or goat anti-rabbit (1:400, Beyotime, China)—for 1.5 h at room temperature.

    Article Title: The lateral habenula contributes to regulation of body temperature
    Article Snippet: It was cut into 20-μm slices using a frozen sectioning machine (LEICA CM1950, Nussloch, Germany). .. The brain tissue sections were washed with PBS three times at room temperature for 10 min each and then incubated in 0.3% Triton X-100 for 30 min. After blocking with a commercial immunostaining blocking solution (Beyotime: P0260) for 1 h, the sections were incubated overnight at 4°C with rat anti-c-Fos primary antibody (Synaptic Systems, 226017, 1:1000), rabbit anti-c-Fos primary antibody (Abcam, ab190289, 1:1000), rabbit anti-Glutamate primary antibody (Sigma, G6642, 1: 200), mouse anti-GABA primary antibody (Sigma, A0310, 1: 200), and rabbit anti-NeuN (CST, 24307s, 1:1000). .. Next day, the slices were incubated for 2 h at room temperature with donkey anti-rabbit IgG secondary antibody (Invitrogen, A21206, H + L, Alexa FluorTM488), goat anti-mouse IgG secondary antibody (Abcam, ab150113, H + L, Alexa FluorTM488), donkey anti-rat IgG secondary antibody (Invitrogen, A11077, H + L, Alexa FluorTM568) and goat anti-rat IgG secondary antibody (Invitrogen, A21247, H + L, Alexa FluorTM647).

    Article Title: Supporting Information
    Article Snippet: .. After 3 days, cells were fixed in PFA for 15 min and incubated with quickblockTM blocking buffer (Beyotime, China) for immunofluorescence staining for 15 min. Primary antibody was added and incubated overnight, including COL I, Scleraxis (Scx), and tenomodulin (Tnmd). .. Subsequently, the appropriate secondary antibodies (Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L) or Cy3-AffiniPure Goat AntiMouse IgG (H+L)) were added.

    Incubation:

    Article Title: Exosomal miR-30a-5p promoted intrahepatic cholangiocarcinoma progression by increasing angiogenesis and vascular permeability dependent on PDCD10
    Article Snippet: .. Then, the gel was transferred to nitrocellulose membranes, blocked with QuickBlockTM Blocking Buffer (Beyotime, China) for 15 minutes, incubated with primary antibody overnight at 4◦C and with secondary antibody at room temperature for 2h. .. The exposed Proteins images were visualized with ECL Plus (EMD Millipore, Billarica, MA, USA).

    Article Title: KLF5-regulated ZDHHC8 reduces chemosensitivity in high-grade serous ovarian cancer through β-catenin signaling
    Article Snippet: Equal amounts of protein (10 μg per sample) were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) on a 12% gel and transferred to PVDF membranes (Millipore, Germany). .. Membranes were blocked with 5% nonfat milk in TBST (Beyotime, Shanghai, China, Cat# P0231) for 1 h at room temperature, followed by overnight incubation at 4 °C with primary antibodies against ZDHHC8 (1:1000, Cat# 27179-1-AP), KLF5 (1:1000, Cat# 21017-1-AP), β-catenin (1:5000, Cat# 51067-2-AP), β-actin (1:5000, Cat# 81115-1-RR), and H3 (1:2000, Cat# 17168-1-AP) (Proteintech, USA). .. After washing, membranes were incubated with HRP-conjugated secondary antibodies (1:5000, ZSGB-Bio, Beijing, China, Cat# ZB-2301) for 2 h at room temperature.

    Article Title: Construction and evaluation of recombinant Lactobacillus plantarum expressing Micropterus salmoides hepcidin.
    Article Snippet: Proteins were transferred to a PVDF membrane (0.22 um; Beyotime, FFP70; China) for 28 min. .. The membrane was blocked with sealing fluid TBST (Beyotime, P0231; China) for 2 h at 37 ◦ C, then incubated with a primary 6 × His-Tag antibody (1:2,000 dilution; Thermo Fisher, HRP66,005; China) overnight at 4 ◦ C. After three 10-min TBST washes with agitation, the membrane was probed with a HRP-conjugated goat anti-rabbit IgG secondary antibody (1:500 dilution; Beyotime, P09478; China) for 1 h at 37 ◦ C. Following three 15-min TBST washes, signals were detected using an ECL chemiluminescence kit (Beyotime, P0018; China). ..

    Article Title: Milk-derived exosomes exert anti-inflammatory activity in lipopolysaccharide-induced RAW264.7 cells by modulating the TLR4/NF-κB and PI3K/AKT signaling pathways
    Article Snippet: Proteins were subsequently transferred from the gel to a PVDF membrane (MilliporeSigma). .. The PVDF membrane was treated with a blocking solution (QuickBlockTM Western; cat. no. P0252; Beyotime Institute of Biotechnology) to inhibit non-specific binding at room temperature for 10 min, then the membranes were incubated at 4 ̊C for 12-16 h with the primary antibodies. ..

    Article Title: Noninvasive method for achieving the regeneration of damaged nerves via ultrasonic nasal drops.
    Article Snippet: .. The membrane was first blocked with QuickBlockTM Blocking Buffer (Beyotime, China) for 30 min, followed by overnight incubation at 4 ◦C with primary antibodies. .. After being washed three times with Western Wash Buffer (Beyotime, China), the membrane was incubated with a secondary antibody—either goat antimouse (1:400, Beyotime, China) or goat anti-rabbit (1:400, Beyotime, China)—for 1.5 h at room temperature.

    Article Title: The lateral habenula contributes to regulation of body temperature
    Article Snippet: It was cut into 20-μm slices using a frozen sectioning machine (LEICA CM1950, Nussloch, Germany). .. The brain tissue sections were washed with PBS three times at room temperature for 10 min each and then incubated in 0.3% Triton X-100 for 30 min. After blocking with a commercial immunostaining blocking solution (Beyotime: P0260) for 1 h, the sections were incubated overnight at 4°C with rat anti-c-Fos primary antibody (Synaptic Systems, 226017, 1:1000), rabbit anti-c-Fos primary antibody (Abcam, ab190289, 1:1000), rabbit anti-Glutamate primary antibody (Sigma, G6642, 1: 200), mouse anti-GABA primary antibody (Sigma, A0310, 1: 200), and rabbit anti-NeuN (CST, 24307s, 1:1000). .. Next day, the slices were incubated for 2 h at room temperature with donkey anti-rabbit IgG secondary antibody (Invitrogen, A21206, H + L, Alexa FluorTM488), goat anti-mouse IgG secondary antibody (Abcam, ab150113, H + L, Alexa FluorTM488), donkey anti-rat IgG secondary antibody (Invitrogen, A11077, H + L, Alexa FluorTM568) and goat anti-rat IgG secondary antibody (Invitrogen, A21247, H + L, Alexa FluorTM647).

    Article Title: Supporting Information
    Article Snippet: .. After 3 days, cells were fixed in PFA for 15 min and incubated with quickblockTM blocking buffer (Beyotime, China) for immunofluorescence staining for 15 min. Primary antibody was added and incubated overnight, including COL I, Scleraxis (Scx), and tenomodulin (Tnmd). .. Subsequently, the appropriate secondary antibodies (Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L) or Cy3-AffiniPure Goat AntiMouse IgG (H+L)) were added.

    Membrane:

    Article Title: Construction and evaluation of recombinant Lactobacillus plantarum expressing Micropterus salmoides hepcidin.
    Article Snippet: Proteins were transferred to a PVDF membrane (0.22 um; Beyotime, FFP70; China) for 28 min. .. The membrane was blocked with sealing fluid TBST (Beyotime, P0231; China) for 2 h at 37 ◦ C, then incubated with a primary 6 × His-Tag antibody (1:2,000 dilution; Thermo Fisher, HRP66,005; China) overnight at 4 ◦ C. After three 10-min TBST washes with agitation, the membrane was probed with a HRP-conjugated goat anti-rabbit IgG secondary antibody (1:500 dilution; Beyotime, P09478; China) for 1 h at 37 ◦ C. Following three 15-min TBST washes, signals were detected using an ECL chemiluminescence kit (Beyotime, P0018; China). ..

    Article Title: Milk-derived exosomes exert anti-inflammatory activity in lipopolysaccharide-induced RAW264.7 cells by modulating the TLR4/NF-κB and PI3K/AKT signaling pathways
    Article Snippet: Proteins were subsequently transferred from the gel to a PVDF membrane (MilliporeSigma). .. The PVDF membrane was treated with a blocking solution (QuickBlockTM Western; cat. no. P0252; Beyotime Institute of Biotechnology) to inhibit non-specific binding at room temperature for 10 min, then the membranes were incubated at 4 ̊C for 12-16 h with the primary antibodies. ..

    Article Title: Noninvasive method for achieving the regeneration of damaged nerves via ultrasonic nasal drops.
    Article Snippet: .. The membrane was first blocked with QuickBlockTM Blocking Buffer (Beyotime, China) for 30 min, followed by overnight incubation at 4 ◦C with primary antibodies. .. After being washed three times with Western Wash Buffer (Beyotime, China), the membrane was incubated with a secondary antibody—either goat antimouse (1:400, Beyotime, China) or goat anti-rabbit (1:400, Beyotime, China)—for 1.5 h at room temperature.

    other:

    Article Title: Rutin ameliorates imiquimod-induced psoriasis-like skin lesions by inhibiting oxidative stress injury and the inflammatory response in mice via the Keap1/Nrf2 signaling pathway.
    Article Snippet: The membranes were blocked by blocking buffer (Beyotime, Cat# P0252, Shanghai, China) for 10 min, and then incubated with the following primary antibodies: Nrf2 (1:1000), Keap1 (1:2000), and β-actin (1:2000) at 4 °C overnight.

    Western Blot:

    Article Title: Milk-derived exosomes exert anti-inflammatory activity in lipopolysaccharide-induced RAW264.7 cells by modulating the TLR4/NF-κB and PI3K/AKT signaling pathways
    Article Snippet: Proteins were subsequently transferred from the gel to a PVDF membrane (MilliporeSigma). .. The PVDF membrane was treated with a blocking solution (QuickBlockTM Western; cat. no. P0252; Beyotime Institute of Biotechnology) to inhibit non-specific binding at room temperature for 10 min, then the membranes were incubated at 4 ̊C for 12-16 h with the primary antibodies. ..

    Binding Assay:

    Article Title: Milk-derived exosomes exert anti-inflammatory activity in lipopolysaccharide-induced RAW264.7 cells by modulating the TLR4/NF-κB and PI3K/AKT signaling pathways
    Article Snippet: Proteins were subsequently transferred from the gel to a PVDF membrane (MilliporeSigma). .. The PVDF membrane was treated with a blocking solution (QuickBlockTM Western; cat. no. P0252; Beyotime Institute of Biotechnology) to inhibit non-specific binding at room temperature for 10 min, then the membranes were incubated at 4 ̊C for 12-16 h with the primary antibodies. ..

    Immunostaining:

    Article Title: The lateral habenula contributes to regulation of body temperature
    Article Snippet: It was cut into 20-μm slices using a frozen sectioning machine (LEICA CM1950, Nussloch, Germany). .. The brain tissue sections were washed with PBS three times at room temperature for 10 min each and then incubated in 0.3% Triton X-100 for 30 min. After blocking with a commercial immunostaining blocking solution (Beyotime: P0260) for 1 h, the sections were incubated overnight at 4°C with rat anti-c-Fos primary antibody (Synaptic Systems, 226017, 1:1000), rabbit anti-c-Fos primary antibody (Abcam, ab190289, 1:1000), rabbit anti-Glutamate primary antibody (Sigma, G6642, 1: 200), mouse anti-GABA primary antibody (Sigma, A0310, 1: 200), and rabbit anti-NeuN (CST, 24307s, 1:1000). .. Next day, the slices were incubated for 2 h at room temperature with donkey anti-rabbit IgG secondary antibody (Invitrogen, A21206, H + L, Alexa FluorTM488), goat anti-mouse IgG secondary antibody (Abcam, ab150113, H + L, Alexa FluorTM488), donkey anti-rat IgG secondary antibody (Invitrogen, A11077, H + L, Alexa FluorTM568) and goat anti-rat IgG secondary antibody (Invitrogen, A21247, H + L, Alexa FluorTM647).

    Immunofluorescence:

    Article Title: Supporting Information
    Article Snippet: .. After 3 days, cells were fixed in PFA for 15 min and incubated with quickblockTM blocking buffer (Beyotime, China) for immunofluorescence staining for 15 min. Primary antibody was added and incubated overnight, including COL I, Scleraxis (Scx), and tenomodulin (Tnmd). .. Subsequently, the appropriate secondary antibodies (Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L) or Cy3-AffiniPure Goat AntiMouse IgG (H+L)) were added.

    Staining:

    Article Title: Supporting Information
    Article Snippet: .. After 3 days, cells were fixed in PFA for 15 min and incubated with quickblockTM blocking buffer (Beyotime, China) for immunofluorescence staining for 15 min. Primary antibody was added and incubated overnight, including COL I, Scleraxis (Scx), and tenomodulin (Tnmd). .. Subsequently, the appropriate secondary antibodies (Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L) or Cy3-AffiniPure Goat AntiMouse IgG (H+L)) were added.



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