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qbase quantification software  (Biogazelle)

 
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    Structured Review

    Biogazelle qbase quantification software
    Qbase Quantification Software, supplied by Biogazelle, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/qbase+quantification+software/qbase+relative+quantification+software/pm27064638-79-18-21
    Average 90 stars, based on 1 article reviews
    qbase quantification software - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Quantitative Proteomics:

    Article Title: An amino acid-based amphoteric liposomal delivery system for systemic administration of siRNA.
    Article Snippet: Quantitative real-time PCR to detect mRNA expression levels was carried out in a 384-well format using the PerfeCta SYBR Green FastMix (Quanta Biosciences), using the Applied Biosystems 7900HT platform (Applied Biosystems, Foster City, CA). .. Data was then analyzed using ΔΔCT method in qBASE relative quantification software (Biogazelle, Belgium). .. The endogenous genes, GAPDH and PPIA, were selected for use in mRNA normalization based on geNorm analysis (http://medgen.ugent.be/~jvdesomp/ genorm).

    Article Title: Development of an experimental model of maternal allergic asthma during pregnancy
    Article Snippet: .. The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3-monooxygenase (YWAHZ)) were chosen (Duffield et al. 2009; Passmore et al. 2009; Wang et al. 2013; Hellemans & Vandesompele, 2014) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al. 2007) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al. 2002; Soo et al. 2012; Hellemans & Vandesompele, 2014). .. The relative expression of messenger ribonucleic acid (mRNA) transcripts of molecules involved in physiological hypertrophy (IGF1, IGF1R and IGF2; Gentili et al. 2009; Zhang et al. 2010), pathological hypertrophy (ANP, BNP, IGF2R and AT1R; Lie et al. 2013, 2014; Zhang et al. 2013), cortisol availability (GR, MR, 11βHSD1 and 11βHSD2; Gentili et al. 2009), inflammation (TNFα and IL1β), fibrosis (TGFβ, collagen type II, MMP 2, TIMP 1, TIMP 2 and TIMP 3; Zhang et al. 2010; Wang et al. 2015), proliferation (PCNA and Ki67), reactive oxygen species (HO1) and glucose and fatty acid uptake (GLUT1, GLUT4, FATP1 and CPT1; Gentili et al. 2009; Wang et al. 2013; Nicholas et al. 2014) were measured by quantitative real-time RT-PCR (qRT-PCR) (Table 1) using Fast SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on a ViiA7 Fast Real-time PCR system (Applied Biosystems) as previously described (Wang et al. 2011; Soo et al. 2012; McGillick et al. 2013).

    Article Title: RNAi-based therapeutics targeting survivin and PLK1 for treatment of bladder cancer.
    Article Snippet: Quantitative real-time PCR was carried out in a 384-well format to detect survivin or PLK1 expression levels using the Quanta Perfecta SYBR Green Fast Mix with ROX (Quanta Biosciences, Gaithersburg, MD), on the Applied Biosystems 7900HT platform (Applied Biosystems). .. Data were analyzed using the ΔΔCT method in qBASE relative quantification software (Biogazelle, Ghent, Belgium). .. Endogenous housekeeping genes, HPRT1, HSPCB, and TBP were selected based on geNorm analysis (http://medgen.ugent.be/~jvdesomp/genorm).

    Article Title: Development of an experimental model of maternal allergic asthma during pregnancy
    Article Snippet: .. The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3‐monooxygenase (YWAHZ)) were chosen (Duffield et al . 2009 ; Passmore et al . 2009 ; Wang et al . 2013 ; Hellemans & Vandesompele, 2014 ) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al . 2007 ) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al . 2002 ; Soo et al . 2012 ; Hellemans & Vandesompele, 2014 ). .. The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3‐monooxygenase (YWAHZ)) were chosen (Duffield et al . 2009 ; Passmore et al . 2009 ; Wang et al . 2013 ; Hellemans & Vandesompele, 2014 ) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al . 2007 ) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al . 2002 ; Soo et al . 2012 ; Hellemans & Vandesompele, 2014 ).

    Software:

    Article Title: An amino acid-based amphoteric liposomal delivery system for systemic administration of siRNA.
    Article Snippet: Quantitative real-time PCR to detect mRNA expression levels was carried out in a 384-well format using the PerfeCta SYBR Green FastMix (Quanta Biosciences), using the Applied Biosystems 7900HT platform (Applied Biosystems, Foster City, CA). .. Data was then analyzed using ΔΔCT method in qBASE relative quantification software (Biogazelle, Belgium). .. The endogenous genes, GAPDH and PPIA, were selected for use in mRNA normalization based on geNorm analysis (http://medgen.ugent.be/~jvdesomp/ genorm).

    Article Title: Development of an experimental model of maternal allergic asthma during pregnancy
    Article Snippet: .. The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3-monooxygenase (YWAHZ)) were chosen (Duffield et al. 2009; Passmore et al. 2009; Wang et al. 2013; Hellemans & Vandesompele, 2014) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al. 2007) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al. 2002; Soo et al. 2012; Hellemans & Vandesompele, 2014). .. The relative expression of messenger ribonucleic acid (mRNA) transcripts of molecules involved in physiological hypertrophy (IGF1, IGF1R and IGF2; Gentili et al. 2009; Zhang et al. 2010), pathological hypertrophy (ANP, BNP, IGF2R and AT1R; Lie et al. 2013, 2014; Zhang et al. 2013), cortisol availability (GR, MR, 11βHSD1 and 11βHSD2; Gentili et al. 2009), inflammation (TNFα and IL1β), fibrosis (TGFβ, collagen type II, MMP 2, TIMP 1, TIMP 2 and TIMP 3; Zhang et al. 2010; Wang et al. 2015), proliferation (PCNA and Ki67), reactive oxygen species (HO1) and glucose and fatty acid uptake (GLUT1, GLUT4, FATP1 and CPT1; Gentili et al. 2009; Wang et al. 2013; Nicholas et al. 2014) were measured by quantitative real-time RT-PCR (qRT-PCR) (Table 1) using Fast SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on a ViiA7 Fast Real-time PCR system (Applied Biosystems) as previously described (Wang et al. 2011; Soo et al. 2012; McGillick et al. 2013).

    Article Title: Association of left ventricular structure and function with peripheral blood mitochondrial DNA content in a general population.
    Article Snippet: a Research Unit Hypertension and Cardiovascular Epidemiology, KU Leuven Department of Cardiovascular Sciences, University of Leuven, Leuven, Belgium b Hypertension Division, Department of Internal Medicine, University Clinical Centre Ljubljana, Slovenia c Centre for Environmental Sciences, Hasselt University, Diepenbeek, Belgium d KU Leuven Department of Public Health, Occupational and Environmental Medicine, University of Leuven, Leuven, Belgium

    Article Title: RNAi-based therapeutics targeting survivin and PLK1 for treatment of bladder cancer.
    Article Snippet: Quantitative real-time PCR was carried out in a 384-well format to detect survivin or PLK1 expression levels using the Quanta Perfecta SYBR Green Fast Mix with ROX (Quanta Biosciences, Gaithersburg, MD), on the Applied Biosystems 7900HT platform (Applied Biosystems). .. Data were analyzed using the ΔΔCT method in qBASE relative quantification software (Biogazelle, Ghent, Belgium). .. Endogenous housekeeping genes, HPRT1, HSPCB, and TBP were selected based on geNorm analysis (http://medgen.ugent.be/~jvdesomp/genorm).

    Article Title: Peripheral blood mitochondrial DNA content in relation to circulating metabolites and inflammatory markers: A population study
    Article Snippet: .. Cycle threshold (Ct) values of the 2 mitochondrial DNA sequences were normalized relative to the nuclear gene using qBase quantification software (Biogazelle, Zwijnaarde, BE) [ ]. ..

    Article Title: Correlates of Peripheral Blood Mitochondrial DNA Content in a General Population
    Article Snippet: We amplified the target sequences in a 7900HT Fast Real-Time PCR thermal cycler (Applied Biosystems, Inc.). .. Cycle threshold ( C t ) values of the 2 mitochondrial DNA sequences were normalized relative to the nuclear gene using qBase quantification software (Biogazelle NV, Zwijnaarde, Belgium). ..

    Article Title: Development of an experimental model of maternal allergic asthma during pregnancy
    Article Snippet: .. The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3‐monooxygenase (YWAHZ)) were chosen (Duffield et al . 2009 ; Passmore et al . 2009 ; Wang et al . 2013 ; Hellemans & Vandesompele, 2014 ) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al . 2007 ) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al . 2002 ; Soo et al . 2012 ; Hellemans & Vandesompele, 2014 ). .. The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3‐monooxygenase (YWAHZ)) were chosen (Duffield et al . 2009 ; Passmore et al . 2009 ; Wang et al . 2013 ; Hellemans & Vandesompele, 2014 ) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al . 2007 ) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al . 2002 ; Soo et al . 2012 ; Hellemans & Vandesompele, 2014 ).

    Expressing:

    Article Title: Development of an experimental model of maternal allergic asthma during pregnancy
    Article Snippet: .. The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3-monooxygenase (YWAHZ)) were chosen (Duffield et al. 2009; Passmore et al. 2009; Wang et al. 2013; Hellemans & Vandesompele, 2014) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al. 2007) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al. 2002; Soo et al. 2012; Hellemans & Vandesompele, 2014). .. The relative expression of messenger ribonucleic acid (mRNA) transcripts of molecules involved in physiological hypertrophy (IGF1, IGF1R and IGF2; Gentili et al. 2009; Zhang et al. 2010), pathological hypertrophy (ANP, BNP, IGF2R and AT1R; Lie et al. 2013, 2014; Zhang et al. 2013), cortisol availability (GR, MR, 11βHSD1 and 11βHSD2; Gentili et al. 2009), inflammation (TNFα and IL1β), fibrosis (TGFβ, collagen type II, MMP 2, TIMP 1, TIMP 2 and TIMP 3; Zhang et al. 2010; Wang et al. 2015), proliferation (PCNA and Ki67), reactive oxygen species (HO1) and glucose and fatty acid uptake (GLUT1, GLUT4, FATP1 and CPT1; Gentili et al. 2009; Wang et al. 2013; Nicholas et al. 2014) were measured by quantitative real-time RT-PCR (qRT-PCR) (Table 1) using Fast SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on a ViiA7 Fast Real-time PCR system (Applied Biosystems) as previously described (Wang et al. 2011; Soo et al. 2012; McGillick et al. 2013).

    Article Title: Development of an experimental model of maternal allergic asthma during pregnancy
    Article Snippet: .. The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3‐monooxygenase (YWAHZ)) were chosen (Duffield et al . 2009 ; Passmore et al . 2009 ; Wang et al . 2013 ; Hellemans & Vandesompele, 2014 ) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al . 2007 ) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al . 2002 ; Soo et al . 2012 ; Hellemans & Vandesompele, 2014 ). .. The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3‐monooxygenase (YWAHZ)) were chosen (Duffield et al . 2009 ; Passmore et al . 2009 ; Wang et al . 2013 ; Hellemans & Vandesompele, 2014 ) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al . 2007 ) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al . 2002 ; Soo et al . 2012 ; Hellemans & Vandesompele, 2014 ).

    other:

    Article Title: RNAi-based Therapeutics Targeting Survivin and PLK1 for Treatment of Bladder Cancer
    Article Snippet: Particle size and zeta-potential of the liposomes were determined using a Malvern Nano ZS particle sizer (Malvern, Worcestershire, UK).



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    Image Search Results


    Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).

    Journal: The Journal of Physiology

    Article Title: Development of an experimental model of maternal allergic asthma during pregnancy

    doi: 10.1113/JP270752

    Figure Lengend Snippet: Image analysis of Masson's trichrome‐stained lung sections for the quantification of airway smooth muscle (ASM; C) and collagen content (D) relative to basement membrane (BM) length. Anti‐tryptase mast cell+ staining (white arrows; degranulated mast cells indicated with open arrows) in lung tissue of control (E) and allergic pregnant sheep (F), with cell counts presented in airway parenchyma (G) and within the airway wall (H). Differences between control and allergic animals are shown by **P < 0.01. Quantitative data are presented as data from individual animals for control (open circles) and allergic (filled circles) sheep, with means ± SEM (n = 5/group).

    Article Snippet: The reference genes (β actin, hypoxanthine phosphoribosyltransferase 1 and tyrosine 3‐monooxygenase (YWAHZ)) were chosen (Duffield et al . 2009 ; Passmore et al . 2009 ; Wang et al . 2013 ; Hellemans & Vandesompele, 2014 ) based on expression analysis using the geNorm component of the qBase (Biogazelle, Zwinjnaarde, Belgium) relative quantification analysis software (Hellemans et al . 2007 ) because their expression was stable across samples (maximum value, factor = 0.3–0.4; Vandesompele et al . 2002 ; Soo et al . 2012 ; Hellemans & Vandesompele, 2014 ).

    Techniques: Staining, Membrane, Control