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BIOTAGE pyrosequencing
Pyrosequencing, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pyrosequencing+genotyping+method/pyrosequencing/pmc03518451-175-15-13
Average 90 stars, based on 1 article reviews
pyrosequencing - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Variants in the DYX2 locus are associated with altered brain activation in reading-related brain regions in subjects with reading disability
Article Snippet: Genotyping methods by TaqMan Assay-on-Demand (Applied Biosystems, Foster City, CA), TaqMan Assay-by-Design, Pyrosequencing (Biotage, Uppsala), fluorescent dideoxynucleotide DNA sequencing with sequence trace analysis by Mutation Surveyor v2.6 (SoftGenetics, State College, PA), and allele specific PCR for the {"type":"entrez-nucleotide","attrs":{"text":"BV677278","term_id":"77735342","term_text":"BV677278"}} BV677278 microdeletion/complex tandem repeat (rsdel and rsSTR) are summarized in Supplementary Tables S3-S4 ( Meng et al. 2005 ; Meng et al. 2011 ). presents the multiple alleles (1-10) of the {"type":"entrez-nucleotide","attrs":{"text":"BV677278","term_id":"77735342","term_text":"BV677278"}} BV677278 complex tandem repeat alleles observed in this sample. table ft1 table-wrap mode=article t1 caption a4 {"type":"entrez-nucleotide","attrs":{"text":"BV677278","term_id":"77735342","term_text":"BV677278"}} BV677278 complex tandem repeat in DCDC2

Article Title: Gender effects of single nucleotide polymorphisms and miRNAs targeting clock-genes in metastatic colorectal cancer patients (mCRC)
Article Snippet: Primers for PCR amplification and pyrosequencing were chosen by pyromark software (Biotage) and are listed in supplementary Table S4.

Article Title: Epigenetic regulation of serotonin transporter expression and behavior in infant rhesus macaques
Article Snippet: Amplicons were subjected to pyrosequencing (Tost & Gut 2007; Biotage Inc., Uppsala, Sweden).

Article Title: High folate intake is related to better academic achievement in Swedish adolescents.
Article Snippet: BACKGROUND: Adolescents are vulnerable to increased plasma total homocysteine (tHcy) and to insufficient folate status.. Folate status and Hcy metabolism are linked to cognitive functions, but academic achievement by adolescents has not been studied in this respect.. OBJECTIVE: To assess a possible link between academic achievement in adolescents and tHcy and its determinants, dietary folate intake, MTHFR 677 TT homozygosity, and socioeconomic status (SES).

Article Title: Heat and pH measurement for sequencing of DNA
Article Snippet: One approach to sequencing by detecting pyrophosphate is the pyrosequencing method, which is being commercialized by Biotage and 454 Life Sciences (a subsidiary of CuraGen Corp., Branford, Conn.).

Biomarker Discovery:

Article Title: Global Promoter Methylation Analysis Reveals Novel Candidate Tumor Suppressor Genes in Natural Killer Cell Lymphoma
Article Snippet: .. Locus specific validation of BIM, ASNS and DAPK1 promoter methylation with pyrosequencing and q-MSP We evaluated the validity of the MSCC technique by comparing the promoter methylation profile of the NKCL cases with previously published Biotage pyrosequencing results (9) for the HpaII site at 747 bp upstream of the PRDM1α TSS. .. MSCC and pyrosequencing results were both available for this site in 13 malignant NK samples and two (resting and IL2-activated NK cells for Biotage pyrosequencing) or three (technical triplicate of IL2-activated NK cells for MSCC) normal NK samples.

Methylation:

Article Title: Global Promoter Methylation Analysis Reveals Novel Candidate Tumor Suppressor Genes in Natural Killer Cell Lymphoma
Article Snippet: .. Locus specific validation of BIM, ASNS and DAPK1 promoter methylation with pyrosequencing and q-MSP We evaluated the validity of the MSCC technique by comparing the promoter methylation profile of the NKCL cases with previously published Biotage pyrosequencing results (9) for the HpaII site at 747 bp upstream of the PRDM1α TSS. .. MSCC and pyrosequencing results were both available for this site in 13 malignant NK samples and two (resting and IL2-activated NK cells for Biotage pyrosequencing) or three (technical triplicate of IL2-activated NK cells for MSCC) normal NK samples.



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Summary of the results obtained using three methods on clinical samples.

Journal: International Journal of Molecular Sciences

Article Title: Mouthwash-Based Highly Sensitive Pyro-Genotyping for Nine Sexually Transmitted Human Papilloma Virus Genotypes

doi: 10.3390/ijms21103697

Figure Lengend Snippet: Summary of the results obtained using three methods on clinical samples.

Article Snippet: We could identify all nine HPV genotypes targeted by the HPV vaccines using the pyrosequencing-based genotyping method and a specific reverse unique probe [ , , ].

Techniques: Amplification