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genotyping method pyrosequencing  (Pyrosequencing Inc)

 
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    Pyrosequencing Inc genotyping method pyrosequencing
    Genotyping Method Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pyrosequencing+genotyping+method/pyrosequencingtm/pm18299791-107-9-12
    Average 90 stars, based on 1 article reviews
    genotyping method pyrosequencing - by Bioz Stars, 2026-11
    90/100 stars

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    other:

    Article Title: Ten ring-B aromatized ergosterols from Aspergillus spectabilis
    Article Snippet: [ABSTRACT] Variations in herb dosage due to species adulteration and dosing inaccuracies can substantially affect clinical safety and efficacy.. Accurate species quantification remains challenging, as current methods often yield inconsistent results.. This study introduces a novel pyrosequencing-based technique, termed herb molecular quantification (Herb-Q), designed to precisely quantify herbal products.

    Article Title: Spectrum of BCR-ABL mutations in Azerbaijanian imatinib-resistant patients with chronic myeloid leukemia
    Article Snippet: During a 5.5 years Pyrosequencing-based analysis (January 2014 to May 2019) of BCR-ABL1 kinase domain mutations, at least one point mutation was identified in 22 of 163 patients (13.4%). shows the frequency of the mutations, as well as mutations leading to high levels of resistance to second-line TKIs [ ].

    Article Title: Distribution and insecticide resistance profile of the major malaria vector Anopheles funestus group across the African continent.
    Article Snippet: Pyrosequencing genotyping and sequencing of the VGSC gene did not detect the common L1014F mutation in the field-collected An. funestus across Africa (Irving & Wondji, 2017).

    Article Title: Multimodal magnetic resonance imaging (MRI) of juvenile and adult diffuse hemispheric gliomas with H3 G34-mutation: a case description
    Article Snippet: In addition, pyrosequencing-based analysis revealed a H3.3 G34R mutation ( ).

    Article Title: A Combination of Alectinib and DNA-Demethylating Agents Synergistically Inhibits Anaplastic-Lymphoma-Kinase-Positive Anaplastic Large-Cell Lymphoma Cell Proliferation
    Article Snippet: The degree of methylation at CpG sites in the promoter-proximal region of LINE-1 was measured using a pyrosequencing-based assay, as described previously [ ].

    Article Title: Mitochondria-Associated Protein FgNdk1 Regulates the Development, Pathogenicity, and SDHI Fungicide Sensitivity of Fusarium graminearum by Interacting with Succinate Dehydrogenase.
    Article Snippet: Nucleoside diphosphate kinase (NDK) plays an important role in many cellular processes in all organisms.. In this study, we functionally characterized a nucleoside diphosphate kinase (FgNdk1) in Fusarium graminearum, a causal agent of Fusarium head blight (FHB).. FgNdk1 was involved in the generation of energy in the electron-transfer chain by interacting with succinate dehydrogenase (FgSdhA, FgSdhC1, and FgSdhC2).

    Article Title: Mapping and Characterization of Target-Site Resistance to Cyclic Ketoenol Insecticides in Cabbage Whiteflies, Aleyrodes proletella (Hemiptera: Aleyrodidae)
    Article Snippet: Strain 1/19 was discontinued after we conducted spiromesifen and spirotetramat bioassays (incl. genotyping by pyrosequencing).

    Hybridization:

    Article Title: Screening for chemotherapy resistance in human haploid cells
    Article Snippet: .. PyrosequencingTM Analysis (Pyrosequencing, Inc. Westborough, MA, USA): This technique is based on the hybridization of a sequencing primer to a single stranded, PCR-amplified, DNA template in the presence of DNA polymerase, ATP sulfurylase, luciferase and apyrase enzymes and the adenosine 5′ phosphosulfate (APS) and luciferin substrates. .. In the second step the first of four deoxynucleotide triphosphates (dNTP) is added to the reaction and the DNA polymerase catalyzes the incorporation of the deoxynucleotide triphosphate into the DNA strand, if it is complementary to the base in the template strand.

    Sequencing:

    Article Title: Screening for chemotherapy resistance in human haploid cells
    Article Snippet: .. PyrosequencingTM Analysis (Pyrosequencing, Inc. Westborough, MA, USA): This technique is based on the hybridization of a sequencing primer to a single stranded, PCR-amplified, DNA template in the presence of DNA polymerase, ATP sulfurylase, luciferase and apyrase enzymes and the adenosine 5′ phosphosulfate (APS) and luciferin substrates. .. In the second step the first of four deoxynucleotide triphosphates (dNTP) is added to the reaction and the DNA polymerase catalyzes the incorporation of the deoxynucleotide triphosphate into the DNA strand, if it is complementary to the base in the template strand.

    Polymerase Chain Reaction:

    Article Title: Screening for chemotherapy resistance in human haploid cells
    Article Snippet: .. PyrosequencingTM Analysis (Pyrosequencing, Inc. Westborough, MA, USA): This technique is based on the hybridization of a sequencing primer to a single stranded, PCR-amplified, DNA template in the presence of DNA polymerase, ATP sulfurylase, luciferase and apyrase enzymes and the adenosine 5′ phosphosulfate (APS) and luciferin substrates. .. In the second step the first of four deoxynucleotide triphosphates (dNTP) is added to the reaction and the DNA polymerase catalyzes the incorporation of the deoxynucleotide triphosphate into the DNA strand, if it is complementary to the base in the template strand.

    Luciferase:

    Article Title: Screening for chemotherapy resistance in human haploid cells
    Article Snippet: .. PyrosequencingTM Analysis (Pyrosequencing, Inc. Westborough, MA, USA): This technique is based on the hybridization of a sequencing primer to a single stranded, PCR-amplified, DNA template in the presence of DNA polymerase, ATP sulfurylase, luciferase and apyrase enzymes and the adenosine 5′ phosphosulfate (APS) and luciferin substrates. .. In the second step the first of four deoxynucleotide triphosphates (dNTP) is added to the reaction and the DNA polymerase catalyzes the incorporation of the deoxynucleotide triphosphate into the DNA strand, if it is complementary to the base in the template strand.



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    Summary of the results obtained using three methods on clinical samples.

    Journal: International Journal of Molecular Sciences

    Article Title: Mouthwash-Based Highly Sensitive Pyro-Genotyping for Nine Sexually Transmitted Human Papilloma Virus Genotypes

    doi: 10.3390/ijms21103697

    Figure Lengend Snippet: Summary of the results obtained using three methods on clinical samples.

    Article Snippet: We could identify all nine HPV genotypes targeted by the HPV vaccines using the pyrosequencing-based genotyping method and a specific reverse unique probe [ , , ].

    Techniques: Amplification