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digestion buffer (proprietary) (Thermo Fisher)

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Structured Review

Thermo Fisher digestion buffer (proprietary)
Digestion Buffer (Proprietary), supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proprietary+buffer/digestion+buffer/pm40324686-60-20-9
Average 90 stars, based on 1 article reviews

digestion buffer (proprietary) - by Bioz Stars, 2026-10

90/100 stars

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Cell Culture:

Article Title: iSuRe-HadCre is an essential tool for effective conditional genetics.
Article Snippet: .. In a cell culture hood, lungs were removed from PBS and then chopped into small fragments with scissors and placed in 10 ml of digestion buffer containing DPBS (Thermofisher 14040141) with Liberase TL (0.14 Wunsch units / ml; Merck 5401020001) and 1 × antibiotic / antimycotic (Thermofisher 15240096). .. Tissue in digestion buffer was incubated in a water bath at 37oC for 30min, with mixing every 2–3 min. After this period, the solution was pipetted up and down to break clumps and after 10 ml of culture medium containing DMEM / F12 (Thermofisher 11320033), 15% FBS and 2 × antibiotic / antimycotic solution was added.

Article Title: iSuRe-HadCre is an essential tool for effective conditional genetics
Article Snippet: .. In a cell culture hood, lungs were removed from PBS and then chopped into small fragments with scissors and placed in 10 ml of digestion buffer containing DPBS (Thermofisher 14040141) with Liberase TL (0.14 Wunsch units/ml; Merck 5401020001) and 1× antibiotic/antimycotic (Thermofisher 15240096). .. Tissue in digestion buffer was incubated in a water bath at 37oC for 30min, with mixing every 2–3 min. After this period, the solution was pipetted up and down to break clumps and after 10 ml of culture medium containing DMEM/F12 (Thermofisher 11320033), 15% FBS and 2× antibiotic/antimycotic solution was added.

other:

Article Title: Near UV and visible light-induced site-specific fragmentation of IgG1-based modalities mediated by histidine and Fe(III): a role for intra-domain interactions?
Article Snippet: Photo-stability represents a critical quality attribute for the development of therapeutic proteins where the exposure to near UV or visible light can lead to protein fragmentation.. Here, we compare the photo-stability of three IgG1 based modalities, formulated in histidine (His) buffer containing various levels of Fe(III).. We report a significant difference in the extent of photo-degradation between a high mannose Fc fragment (HMFc), NISTmAb and a fusion protein, Flt-3L-Ig.

Sterility:

Article Title: Corn Steep Liquor as an Auto-Induction Medium for the Production of rhIFN-β-1b Protein in Escherichia coli
Article Snippet: contribution to overall mortality, thereby exerting a significant adverse effect on the country’s economy.. Breast cancer is the predominant form of cancer in Pakistan, representing the primary cause of mortality.. Among the recently reviewed cases, the prevalence of this condition in the female population is 23% (25.1% globally), while it accounts for 16.1% (15% globally) of total cancer-related fatalities.