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proprietary buffer af6 (Amphasys Inc)

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Amphasys Inc proprietary buffer af6
Proprietary Buffer Af6, supplied by Amphasys Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/proprietary+buffer/af6+buffer/pm40617931-241-4-6
Average 90 stars, based on 1 article reviews

proprietary buffer af6 - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Pollen production, pollen viability and autofertility in faba bean ( Vicia faba L.) and their relationship with realized paternal success
Article Snippet: For each sample, the ten closed anthers of a flower bud were transferred into an Eppendorf tube, slightly squeezed to release the pollen grains and suspended into 2 ml of IFC buffer solution (AF 5, Amphasys, Lucerne, Switzerland).

Article Title: Investigating the effects of plasma-activated water on E. coli with the novel application of single-cell impedance flow cytometry.
Article Snippet: AF6 is a proprietary buffer from Amphasys AG49 that contains an ion concentration similar to 0.11x PBS and sucrose.

Article Title: The Development of an Improved Medium for the In Vitro Germination of Corylus avellana L. Pollen
Article Snippet: Samples were then suspended for 10 min in AF6 buffer (Amphasys, Lucerne, Switzerland), rich in mineral salts and sugars, specially designed to have a suitable osmolality for the analysis of pollen grains.

Article Title: Evaluation of methods to assess the quality of cryopreserved Solanaceae pollen.
Article Snippet: Rehydrated pollen was suspended in 1 ml AF6 IFC measurement buffer (Amphasys AG, Switzerland), or 1 ml germination buffer, and filtered through a 50 μm CellTrics filter (Sysmex, Switzerland) into a clean Eppendorf tube.

Article Title: Identification of QTL for reproductive success under heat stress conditions through a tomato MAGIC population
Article Snippet: The anther cones were removed from the flowers, placed in a 2.0 mL Eppendorf tube containing 750 μL of AmphaFluid 6 (AF6) buffer (Amphasys AG, Switzerland), and vigorously scraped along the tube bottom to release pollen until the solution became turbid.

FACS:

Article Title: The role of sucrose in maintaining pollen viability and germinability in Corylus avellana L.: a possible strategy to cope with climate variability.
Article Snippet: In this work, we propose a possible correlation between carbohydrate content in hazelnut pollen (wild type) and viability/ germinability, also in a perspective of adaptation to climate variability.. Samples from four different cultivation fields in Italy showed values of pollen viability characterized by high levels, ranging between 77.3 and 98.4% and a unique trend during the flowering period for each accession.. When subjected to dehydration in controlled environment, pollen reduced the levels of viability to almost zero but recovered the initial values when rehydrated.

Filtration:

Article Title: Original Research Paper
Article Snippet: .. The pollen was suspended in IFC buffer by vortexing and was filtered through 50 μm filter units according to the tobacco pollen size (Filter set, Amphasys), and added 1 ml of AF6 measurement buffer after filtration. .. Pollen samples were loaded onto an 80 μm channel F chip and placed in an Impedance flow cytometer type AmphaZ32 (Amphasys, Switzerland) to determine the viability of the pollens at 2 and/or 12 MHz using the default settings.