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BioSpherix pro co2 carbon dioxide controller
Pro Co2 Carbon Dioxide Controller, supplied by BioSpherix, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pro-co2+controller/pm28588583-47-7-11
Average 86 stars, based on 1 article reviews

pro co2 carbon dioxide controller - by Bioz Stars, 2026-10

86/100 stars

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Incubation:

Article Title: STAT3 and HIF1α cooperatively mediate the transcriptional and physiological responses to hypoxia
Article Snippet: .. 5% oxygen tension was maintained using ProCO 2 (BioSpherix) device and larvae were incubated with low oxygen tension from 3 dpf to 6 dpf. .. Total RNAs were extracted from pools of 20 larvae at 3, 4, 5 and 6 dpf with TRIzol reagent (Thermo Fisher Scientific, 15596018) and incubated at 37 °C for 30 min with RQ1 RNase-Free DNase (Promega, M6101). cDNA synthesis was performed using random primers (Promega, C1181) and M-MLV Reverse Transcriptase RNase H (Solis BioDyne, 06-21-010000) according to the manufacturer’s protocol. qPCRs were performed in triplicate with CybrGreen method by means of Rotor-gene Q (Qiagen) and the 5x HOT FIREPol EvaGreen qPCR Mix Plus (Solis BioDyne, 08-36-00001) and zgapdh and mActnb were used as internal standard in each sample.

Article Title: Overcoming hypoxia-induced tumor radioresistance in non-small cell lung cancer by targeting DNA-dependent protein kinase in combination with carbon ion irradiation.
Article Snippet: .. To assess clonogenic survival under hypoxic conditions, we devised a system to irradiate cells in 96-well plates while incubated in a hypoxia chamber (C-chamber; Biospherix) allowing online monitoring of CO2 and O2 concentrations (ProOx and ProCO2 model; Biospherix) during the entire experiment (Fig. 1a). ..

Irradiation:

other:

Article Title: Mouse Lung Structure and Function after Long-Term Exposure to an Atmospheric Carbon Dioxide Level Predicted by Climate Change Modeling
Article Snippet: Elevated CO2 was maintained using a ProCO2 P120 (BioSpherix Ltd.).

Article Title: A role for heat shock factor 1 in hypercapnia‐induced inhibition of inflammatory cytokine expression
Article Snippet: MH-S cells were obtained from American Type Culture Collection (ATCC;Manassas, VA, USA) and propagated in RPMI-1640 medium (ATCC), supplemented with 10% fetal bovine serum, 1% penicillin-streptomycin, and 0.003% 2-mercaptoethanol at 37°C in humidified 5% CO2/95% air.

Concentration Assay:

Article Title: Neuropeptide signaling regulates the susceptibility of developing C. elegans to anoxia.
Article Snippet: .. Carbon dioxide was introduced into a sealed chamber fitted with a CO2 controller (ProCO2 from BioSpherix, Inc) until its concentration was 10% of room air, as measured by a CO2 sensor in the chamber. ..

Image Search Results

Figure 1. Hypercapnia limits AT2 cell proliferation in 3D culture organoids. (A) Schematic of experiments designed to coculture AT2 cells isolated from SftpCreERT2 R26REYFP mice (SftpcEYFP AT2) and WT mesenchymal cells. Alveolar organoids were switched to normocapnia (5% CO2; NC) or hypercapnia (20% CO2; HC) media on day 7 and cultured until day 21. (B) Representative images of organoid cultures in normocapnia or hypercapnia. Scale bars: 500 μm. (C) Graph depicts the inhibitory effect of hypercapnia on organoid size. Median with interquartile range. n = 8. (D) Graph depicts the effect of hypercapnia exposure for 21 days on colony forming efficiency (CFE). n = 8. (E) Immunofluorescence analysis of SFTPC (AT2 marker) and Podoplanin (AT1 marker) revealed a reduction in AT2 cell proliferation in organoids exposed to hypercapnia for 14 days relative to normocapnia. Nuclear DNA is stained with DAPI. Scale bars: 50 μm. (C) ANOVA plus Sidak’s multiple comparisons test. (D) Student’s t test. *P < 0.05; ***P < 0.001, ****P < 0.0001.

Journal: JCI insight

Article Title: Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells.

doi: 10.1172/jci.insight.159331

Figure Lengend Snippet: Figure 1. Hypercapnia limits AT2 cell proliferation in 3D culture organoids. (A) Schematic of experiments designed to coculture AT2 cells isolated from SftpCreERT2 R26REYFP mice (SftpcEYFP AT2) and WT mesenchymal cells. Alveolar organoids were switched to normocapnia (5% CO2; NC) or hypercapnia (20% CO2; HC) media on day 7 and cultured until day 21. (B) Representative images of organoid cultures in normocapnia or hypercapnia. Scale bars: 500 μm. (C) Graph depicts the inhibitory effect of hypercapnia on organoid size. Median with interquartile range. n = 8. (D) Graph depicts the effect of hypercapnia exposure for 21 days on colony forming efficiency (CFE). n = 8. (E) Immunofluorescence analysis of SFTPC (AT2 marker) and Podoplanin (AT1 marker) revealed a reduction in AT2 cell proliferation in organoids exposed to hypercapnia for 14 days relative to normocapnia. Nuclear DNA is stained with DAPI. Scale bars: 50 μm. (C) ANOVA plus Sidak’s multiple comparisons test. (D) Student’s t test. *P < 0.05; ***P < 0.001, ****P < 0.0001.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 controller (BioSpherix).

Techniques: Isolation, Cell Culture, Immunofluorescence, Marker, Staining

Figure 2. Transcriptomic analysis of isolated AT2 cells reveals inhibition of βcat signaling during hypercapnia. (A) Hypercapnia decreases the number of cells expressing Ki67 in the alveolar region of the adult mouse lung exposed to room air (RA) or 10% CO2 (HC) for 21 days, as revealed by immunoflu- orescence. White arrows indicate SPTPC+Ki67+ AT2 cells. Scale bars: 20 μm. (B) Graph depicting the inhibitory effect of hypercapnia exposure for 21 days on proliferation. RA, n = 4; HC, n = 3 mice. Student’s t test. **P < 0.01. (C–F) Bulk RNA-Seq was performed on flow cytometry sorted AT2 cells from mice breathing RA (n = 6) or exposed to HC. Heatmap shows clustering of differentially expressed genes (FDR q < 0.05) in AT2 cells after 7 (n = 5) or 21 (n = 5) days of hypercapnia exposure. (D and E) Volcano plots. (F) GO biological processes. (G–J) Expression of selected DEG (FDR q < 0.05) regulated by hypercap- nia involved in the Wnt/βcat pathway.

Journal: JCI insight

Article Title: Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells.

doi: 10.1172/jci.insight.159331

Figure Lengend Snippet: Figure 2. Transcriptomic analysis of isolated AT2 cells reveals inhibition of βcat signaling during hypercapnia. (A) Hypercapnia decreases the number of cells expressing Ki67 in the alveolar region of the adult mouse lung exposed to room air (RA) or 10% CO2 (HC) for 21 days, as revealed by immunoflu- orescence. White arrows indicate SPTPC+Ki67+ AT2 cells. Scale bars: 20 μm. (B) Graph depicting the inhibitory effect of hypercapnia exposure for 21 days on proliferation. RA, n = 4; HC, n = 3 mice. Student’s t test. **P < 0.01. (C–F) Bulk RNA-Seq was performed on flow cytometry sorted AT2 cells from mice breathing RA (n = 6) or exposed to HC. Heatmap shows clustering of differentially expressed genes (FDR q < 0.05) in AT2 cells after 7 (n = 5) or 21 (n = 5) days of hypercapnia exposure. (D and E) Volcano plots. (F) GO biological processes. (G–J) Expression of selected DEG (FDR q < 0.05) regulated by hypercap- nia involved in the Wnt/βcat pathway.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 controller (BioSpherix).

Techniques: Isolation, Inhibition, Expressing, RNA Sequencing, Flow Cytometry

Figure 3. Hypercapnia decreases Wnt/βcat signaling in AT2 cells. AT2 cells were isolated from mice exposed to room air (RA) or 10% CO2 (HC) for 21 days. (A) mRNA was isolated, and qPCR was performed. n = 8 mice. (B and C) In situ RNA hybridization showing decreased number of Axin2+ AT2 cells in mice exposed to HC. Yellow arrows indicate Sftpc+Axin2+ AT2 cells. Scale bars: 10 μm. n = 4 mice. (D) Number of lineage-labeled AT2 cells from Axin2CreERT2–TdTom mice determined by flow cytometry. n = 5 mice. Graph shows data from 1 of 3 independent experiments. Student’s t test. *P < 0.05; **P < 0.01.

Journal: JCI insight

Article Title: Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells.

doi: 10.1172/jci.insight.159331

Figure Lengend Snippet: Figure 3. Hypercapnia decreases Wnt/βcat signaling in AT2 cells. AT2 cells were isolated from mice exposed to room air (RA) or 10% CO2 (HC) for 21 days. (A) mRNA was isolated, and qPCR was performed. n = 8 mice. (B and C) In situ RNA hybridization showing decreased number of Axin2+ AT2 cells in mice exposed to HC. Yellow arrows indicate Sftpc+Axin2+ AT2 cells. Scale bars: 10 μm. n = 4 mice. (D) Number of lineage-labeled AT2 cells from Axin2CreERT2–TdTom mice determined by flow cytometry. n = 5 mice. Graph shows data from 1 of 3 independent experiments. Student’s t test. *P < 0.05; **P < 0.01.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 controller (BioSpherix).

Techniques: Isolation, In Situ, Hybridization, Labeling, Flow Cytometry

Figure 4. Hypercapnia increases Wnt5a expression in PDGFRα+ fibroblasts. Lung PDGFRα+ fibroblasts were isolated via flow cytometry cell sorting from mice breathing room air (RA) or exposed to 10% CO2 (HC) for 10 days. (A) Expression of Wnt genes in PDGFRα+ fibroblasts as analyzed by population RNA-Seq. n = 3, with cells isolated from 3 mice in each replicate. #FDR q < 0.05). (B–D) mRNA was isolated, and qPCR was performed. (B) Wnt5a (n = 4). (C) Wnt2 (n = 3). (D) MLg2908 mouse lung fibroblast cells were preincubated in the presence or absence of UO126 (10 μM) or PD98059 (10 μM) for 90 minutes and exposed to media equilibrated to NC (5% CO2) or HC (20% CO2) for 24 hours. n = 3. (B and C) Student’s t test. (D) ANOVA plus Sidak’s multiple comparisons test. *P < 0.05; ** P < 0.01.

Journal: JCI insight

Article Title: Hypercapnia alters stroma-derived Wnt production to limit β-catenin signaling and proliferation in AT2 cells.

doi: 10.1172/jci.insight.159331

Figure Lengend Snippet: Figure 4. Hypercapnia increases Wnt5a expression in PDGFRα+ fibroblasts. Lung PDGFRα+ fibroblasts were isolated via flow cytometry cell sorting from mice breathing room air (RA) or exposed to 10% CO2 (HC) for 10 days. (A) Expression of Wnt genes in PDGFRα+ fibroblasts as analyzed by population RNA-Seq. n = 3, with cells isolated from 3 mice in each replicate. #FDR q < 0.05). (B–D) mRNA was isolated, and qPCR was performed. (B) Wnt5a (n = 4). (C) Wnt2 (n = 3). (D) MLg2908 mouse lung fibroblast cells were preincubated in the presence or absence of UO126 (10 μM) or PD98059 (10 μM) for 90 minutes and exposed to media equilibrated to NC (5% CO2) or HC (20% CO2) for 24 hours. n = 3. (B and C) Student’s t test. (D) ANOVA plus Sidak’s multiple comparisons test. *P < 0.05; ** P < 0.01.

Article Snippet: The atmosphere of the C-Chamber was controlled with a PRO CO2 controller (BioSpherix).

Techniques: Expressing, Isolation, Flow Cytometry, FACS, RNA Sequencing

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