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Promega prl- null vector
Prl Null Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prl-+null+vector/prl+null+vector/pm38954637-300-1-4
Average 90 stars, based on 1 article reviews
prl- null vector - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Detection of Proteins with Viral Suppressor of RNA Interference Activities from Insect-transmitted Viruses Using <i>Drosophila</i> S2 Cells
Article Snippet: The cDNAs of viral proteins were cloned into pAc5.1/V5-His A (pAc) vectors (Invitrogen, Tokyo), and firefly luciferase from pSP-luc vector (Promega, Tokyo) and Renilla luciferase pRL-null vector (Promega) were separately cloned into pMT/V5-His A (pMT) vectors (Invitrogen).


Article Title: MicroRNA-185-5p inhibits hepatic gluconeogenesis and reduces fasting blood glucose levels by suppressing G6Pase
Article Snippet: The entire 3'-untranslated region of mouse G6Pase gene containing either the wildtype or mutated binding sites was cloned and inserted into a pRL-null vector (Promega, USA).

Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids.
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) us ing je tPEI DNA transfec t ion reagent fo l lowing manufacturer’s instructions (Genesee Scientific).

Article Title: YAP represses the TEAD-NF-κB complex and inhibits the growth of clear cell renal cell carcinoma.
Article Snippet: The Hippo pathway is generally understood to inhibit tumor growth by phosphorylating the transcriptional cofactor YAP to sequester it to the cytoplasm and reduce the formation of YAPTEAD transcriptional complexes.. Aberrant activation of YAP occurs in various cancers.. However, we found a tumorsuppressive function of YAP in clear cell renal cell carcinoma (ccRCC).


Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) using jetPEI DNA transfection reagent following manufacturer’s instructions (Genesee Scientific).

Article Title: Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.
Article Snippet: The pRL-null vector (Promega) expressing Renilla luciferase was an internal control.

Expressing:

Article Title: Detection of Proteins with Viral Suppressor of RNA Interference Activities from Insect-transmitted Viruses Using <i>Drosophila</i> S2 Cells
Article Snippet: The cDNAs of viral proteins were cloned into pAc5.1/V5-His A (pAc) vectors (Invitrogen, Tokyo), and firefly luciferase from pSP-luc vector (Promega, Tokyo) and Renilla luciferase pRL-null vector (Promega) were separately cloned into pMT/V5-His A (pMT) vectors (Invitrogen).


Article Title: MicroRNA-185-5p inhibits hepatic gluconeogenesis and reduces fasting blood glucose levels by suppressing G6Pase
Article Snippet: The entire 3'-untranslated region of mouse G6Pase gene containing either the wildtype or mutated binding sites was cloned and inserted into a pRL-null vector (Promega, USA).

Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids.
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) us ing je tPEI DNA transfec t ion reagent fo l lowing manufacturer’s instructions (Genesee Scientific).

Article Title: YAP represses the TEAD-NF-κB complex and inhibits the growth of clear cell renal cell carcinoma.
Article Snippet: The Hippo pathway is generally understood to inhibit tumor growth by phosphorylating the transcriptional cofactor YAP to sequester it to the cytoplasm and reduce the formation of YAPTEAD transcriptional complexes.. Aberrant activation of YAP occurs in various cancers.. However, we found a tumorsuppressive function of YAP in clear cell renal cell carcinoma (ccRCC).


Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) using jetPEI DNA transfection reagent following manufacturer’s instructions (Genesee Scientific).

Article Title: Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.
Article Snippet: The pRL-null vector (Promega) expressing Renilla luciferase was an internal control.

Luciferase:

Article Title: Detection of Proteins with Viral Suppressor of RNA Interference Activities from Insect-transmitted Viruses Using <i>Drosophila</i> S2 Cells
Article Snippet: The cDNAs of viral proteins were cloned into pAc5.1/V5-His A (pAc) vectors (Invitrogen, Tokyo), and firefly luciferase from pSP-luc vector (Promega, Tokyo) and Renilla luciferase pRL-null vector (Promega) were separately cloned into pMT/V5-His A (pMT) vectors (Invitrogen).


Article Title: MicroRNA-185-5p inhibits hepatic gluconeogenesis and reduces fasting blood glucose levels by suppressing G6Pase
Article Snippet: The entire 3'-untranslated region of mouse G6Pase gene containing either the wildtype or mutated binding sites was cloned and inserted into a pRL-null vector (Promega, USA).

Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids.
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) us ing je tPEI DNA transfec t ion reagent fo l lowing manufacturer’s instructions (Genesee Scientific).

Article Title: YAP represses the TEAD-NF-κB complex and inhibits the growth of clear cell renal cell carcinoma.
Article Snippet: The Hippo pathway is generally understood to inhibit tumor growth by phosphorylating the transcriptional cofactor YAP to sequester it to the cytoplasm and reduce the formation of YAPTEAD transcriptional complexes.. Aberrant activation of YAP occurs in various cancers.. However, we found a tumorsuppressive function of YAP in clear cell renal cell carcinoma (ccRCC).


Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) using jetPEI DNA transfection reagent following manufacturer’s instructions (Genesee Scientific).

Article Title: Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.
Article Snippet: The pRL-null vector (Promega) expressing Renilla luciferase was an internal control.

Control:

Article Title: Detection of Proteins with Viral Suppressor of RNA Interference Activities from Insect-transmitted Viruses Using <i>Drosophila</i> S2 Cells
Article Snippet: The cDNAs of viral proteins were cloned into pAc5.1/V5-His A (pAc) vectors (Invitrogen, Tokyo), and firefly luciferase from pSP-luc vector (Promega, Tokyo) and Renilla luciferase pRL-null vector (Promega) were separately cloned into pMT/V5-His A (pMT) vectors (Invitrogen).


Article Title: MicroRNA-185-5p inhibits hepatic gluconeogenesis and reduces fasting blood glucose levels by suppressing G6Pase
Article Snippet: The entire 3'-untranslated region of mouse G6Pase gene containing either the wildtype or mutated binding sites was cloned and inserted into a pRL-null vector (Promega, USA).

Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids.
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) us ing je tPEI DNA transfec t ion reagent fo l lowing manufacturer’s instructions (Genesee Scientific).

Article Title: YAP represses the TEAD-NF-κB complex and inhibits the growth of clear cell renal cell carcinoma.
Article Snippet: The Hippo pathway is generally understood to inhibit tumor growth by phosphorylating the transcriptional cofactor YAP to sequester it to the cytoplasm and reduce the formation of YAPTEAD transcriptional complexes.. Aberrant activation of YAP occurs in various cancers.. However, we found a tumorsuppressive function of YAP in clear cell renal cell carcinoma (ccRCC).


Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) using jetPEI DNA transfection reagent following manufacturer’s instructions (Genesee Scientific).

Article Title: Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.
Article Snippet: The pRL-null vector (Promega) expressing Renilla luciferase was an internal control.

Clone Assay:

Article Title: Detection of Proteins with Viral Suppressor of RNA Interference Activities from Insect-transmitted Viruses Using <i>Drosophila</i> S2 Cells
Article Snippet: The cDNAs of viral proteins were cloned into pAc5.1/V5-His A (pAc) vectors (Invitrogen, Tokyo), and firefly luciferase from pSP-luc vector (Promega, Tokyo) and Renilla luciferase pRL-null vector (Promega) were separately cloned into pMT/V5-His A (pMT) vectors (Invitrogen).


Article Title: MicroRNA-185-5p inhibits hepatic gluconeogenesis and reduces fasting blood glucose levels by suppressing G6Pase
Article Snippet: The entire 3'-untranslated region of mouse G6Pase gene containing either the wildtype or mutated binding sites was cloned and inserted into a pRL-null vector (Promega, USA).

Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids.
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) us ing je tPEI DNA transfec t ion reagent fo l lowing manufacturer’s instructions (Genesee Scientific).

Article Title: YAP represses the TEAD-NF-κB complex and inhibits the growth of clear cell renal cell carcinoma.
Article Snippet: The Hippo pathway is generally understood to inhibit tumor growth by phosphorylating the transcriptional cofactor YAP to sequester it to the cytoplasm and reduce the formation of YAPTEAD transcriptional complexes.. Aberrant activation of YAP occurs in various cancers.. However, we found a tumorsuppressive function of YAP in clear cell renal cell carcinoma (ccRCC).


Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) using jetPEI DNA transfection reagent following manufacturer’s instructions (Genesee Scientific).

Article Title: Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.
Article Snippet: The pRL-null vector (Promega) expressing Renilla luciferase was an internal control.

Binding Assay:

Article Title: Detection of Proteins with Viral Suppressor of RNA Interference Activities from Insect-transmitted Viruses Using <i>Drosophila</i> S2 Cells
Article Snippet: The cDNAs of viral proteins were cloned into pAc5.1/V5-His A (pAc) vectors (Invitrogen, Tokyo), and firefly luciferase from pSP-luc vector (Promega, Tokyo) and Renilla luciferase pRL-null vector (Promega) were separately cloned into pMT/V5-His A (pMT) vectors (Invitrogen).


Article Title: MicroRNA-185-5p inhibits hepatic gluconeogenesis and reduces fasting blood glucose levels by suppressing G6Pase
Article Snippet: The entire 3'-untranslated region of mouse G6Pase gene containing either the wildtype or mutated binding sites was cloned and inserted into a pRL-null vector (Promega, USA).

Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids.
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) us ing je tPEI DNA transfec t ion reagent fo l lowing manufacturer’s instructions (Genesee Scientific).

Article Title: YAP represses the TEAD-NF-κB complex and inhibits the growth of clear cell renal cell carcinoma.
Article Snippet: The Hippo pathway is generally understood to inhibit tumor growth by phosphorylating the transcriptional cofactor YAP to sequester it to the cytoplasm and reduce the formation of YAPTEAD transcriptional complexes.. Aberrant activation of YAP occurs in various cancers.. However, we found a tumorsuppressive function of YAP in clear cell renal cell carcinoma (ccRCC).


Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) using jetPEI DNA transfection reagent following manufacturer’s instructions (Genesee Scientific).

Article Title: Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.
Article Snippet: The pRL-null vector (Promega) expressing Renilla luciferase was an internal control.

Transfection:

Article Title: Detection of Proteins with Viral Suppressor of RNA Interference Activities from Insect-transmitted Viruses Using <i>Drosophila</i> S2 Cells
Article Snippet: The cDNAs of viral proteins were cloned into pAc5.1/V5-His A (pAc) vectors (Invitrogen, Tokyo), and firefly luciferase from pSP-luc vector (Promega, Tokyo) and Renilla luciferase pRL-null vector (Promega) were separately cloned into pMT/V5-His A (pMT) vectors (Invitrogen).


Article Title: MicroRNA-185-5p inhibits hepatic gluconeogenesis and reduces fasting blood glucose levels by suppressing G6Pase
Article Snippet: The entire 3'-untranslated region of mouse G6Pase gene containing either the wildtype or mutated binding sites was cloned and inserted into a pRL-null vector (Promega, USA).

Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids.
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) us ing je tPEI DNA transfec t ion reagent fo l lowing manufacturer’s instructions (Genesee Scientific).

Article Title: YAP represses the TEAD-NF-κB complex and inhibits the growth of clear cell renal cell carcinoma.
Article Snippet: The Hippo pathway is generally understood to inhibit tumor growth by phosphorylating the transcriptional cofactor YAP to sequester it to the cytoplasm and reduce the formation of YAPTEAD transcriptional complexes.. Aberrant activation of YAP occurs in various cancers.. However, we found a tumorsuppressive function of YAP in clear cell renal cell carcinoma (ccRCC).


Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) using jetPEI DNA transfection reagent following manufacturer’s instructions (Genesee Scientific).

Article Title: Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.
Article Snippet: The pRL-null vector (Promega) expressing Renilla luciferase was an internal control.

Construct:

Article Title: Detection of Proteins with Viral Suppressor of RNA Interference Activities from Insect-transmitted Viruses Using <i>Drosophila</i> S2 Cells
Article Snippet: The cDNAs of viral proteins were cloned into pAc5.1/V5-His A (pAc) vectors (Invitrogen, Tokyo), and firefly luciferase from pSP-luc vector (Promega, Tokyo) and Renilla luciferase pRL-null vector (Promega) were separately cloned into pMT/V5-His A (pMT) vectors (Invitrogen).


Article Title: MicroRNA-185-5p inhibits hepatic gluconeogenesis and reduces fasting blood glucose levels by suppressing G6Pase
Article Snippet: The entire 3'-untranslated region of mouse G6Pase gene containing either the wildtype or mutated binding sites was cloned and inserted into a pRL-null vector (Promega, USA).

Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids.
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) us ing je tPEI DNA transfec t ion reagent fo l lowing manufacturer’s instructions (Genesee Scientific).

Article Title: YAP represses the TEAD-NF-κB complex and inhibits the growth of clear cell renal cell carcinoma.
Article Snippet: The Hippo pathway is generally understood to inhibit tumor growth by phosphorylating the transcriptional cofactor YAP to sequester it to the cytoplasm and reduce the formation of YAPTEAD transcriptional complexes.. Aberrant activation of YAP occurs in various cancers.. However, we found a tumorsuppressive function of YAP in clear cell renal cell carcinoma (ccRCC).


Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) using jetPEI DNA transfection reagent following manufacturer’s instructions (Genesee Scientific).

Article Title: Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.
Article Snippet: The pRL-null vector (Promega) expressing Renilla luciferase was an internal control.

Sequencing:

Article Title: Detection of Proteins with Viral Suppressor of RNA Interference Activities from Insect-transmitted Viruses Using <i>Drosophila</i> S2 Cells
Article Snippet: The cDNAs of viral proteins were cloned into pAc5.1/V5-His A (pAc) vectors (Invitrogen, Tokyo), and firefly luciferase from pSP-luc vector (Promega, Tokyo) and Renilla luciferase pRL-null vector (Promega) were separately cloned into pMT/V5-His A (pMT) vectors (Invitrogen).


Article Title: MicroRNA-185-5p inhibits hepatic gluconeogenesis and reduces fasting blood glucose levels by suppressing G6Pase
Article Snippet: The entire 3'-untranslated region of mouse G6Pase gene containing either the wildtype or mutated binding sites was cloned and inserted into a pRL-null vector (Promega, USA).

Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids.
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) us ing je tPEI DNA transfec t ion reagent fo l lowing manufacturer’s instructions (Genesee Scientific).

Article Title: YAP represses the TEAD-NF-κB complex and inhibits the growth of clear cell renal cell carcinoma.
Article Snippet: The Hippo pathway is generally understood to inhibit tumor growth by phosphorylating the transcriptional cofactor YAP to sequester it to the cytoplasm and reduce the formation of YAPTEAD transcriptional complexes.. Aberrant activation of YAP occurs in various cancers.. However, we found a tumorsuppressive function of YAP in clear cell renal cell carcinoma (ccRCC).


Article Title: Expression of long noncoding RNA Xist is induced by glucocorticoids
Article Snippet: MSCs from 6-month-old C57BL/6 mice were transiently transfected with Xist1.2-Luc reporter construct (custom-built and sequence confirmed, VectorBuilder Inc., Chicago, IL) and the internal control pRL-null vector (Promega Corporation) using jetPEI DNA transfection reagent following manufacturer’s instructions (Genesee Scientific).

Article Title: Regulation of the Hippo/YAP axis by CXCR7 in the tumorigenesis of gastric cancer.
Article Snippet: The pRL-null vector (Promega) expressing Renilla luciferase was an internal control.



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a) Description of the different Cx43 mRNA 3′UTR and 5′UTR constructs used in this experiment. All constructs, except the <t>pGL3</t> basic vector, contain the SV40 promoter (SV40-P) and the Luciferase coding region (pGL3-pr) in addition to the 3′UTR (pGL3-3′UTR) and/or the 5′UTR (pGL3-5′UTR) full length sequences. b) Luciferase assay using Cx43 mRNA 3′UTR and 5′UTR constructs in NIH3T3 Neo cells. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05).
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Image Search Results


a) Description of the different Cx43 mRNA 3′UTR and 5′UTR constructs used in this experiment. All constructs, except the pGL3 basic vector, contain the SV40 promoter (SV40-P) and the Luciferase coding region (pGL3-pr) in addition to the 3′UTR (pGL3-3′UTR) and/or the 5′UTR (pGL3-5′UTR) full length sequences. b) Luciferase assay using Cx43 mRNA 3′UTR and 5′UTR constructs in NIH3T3 Neo cells. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05).

Journal: PLoS ONE

Article Title: Post-Transcriptional Regulation of Connexin43 in H-Ras-Transformed Cells

doi: 10.1371/journal.pone.0058500

Figure Lengend Snippet: a) Description of the different Cx43 mRNA 3′UTR and 5′UTR constructs used in this experiment. All constructs, except the pGL3 basic vector, contain the SV40 promoter (SV40-P) and the Luciferase coding region (pGL3-pr) in addition to the 3′UTR (pGL3-3′UTR) and/or the 5′UTR (pGL3-5′UTR) full length sequences. b) Luciferase assay using Cx43 mRNA 3′UTR and 5′UTR constructs in NIH3T3 Neo cells. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05).

Article Snippet: The luciferase vectors pGL3 (basic promoterless vector containing firefly luciferase), pGL3-pr (bearing an SV40 promoter) and pRL-Null (promoterless vector containing Renilla reniformis luciferase) were purchased from Promega (Madison, WI).

Techniques: Construct, Plasmid Preparation, Luciferase

a) Localization of the different Cx43 3′UTR constructs used in this work. All constructs contain the SV40 promoter (SV40-P) and the Luciferase coding region in addition to the pGL3 polyadenylation signal after the 3′UTR segments (not shown in graphics). The position of a putative polyadenylation signal is indicated. The S1516 region is shown in light grey. b, c) Luciferase assay of the first set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. d, e) Luciferase assay of the second set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05, reported to the pGL3-pr construct).

Journal: PLoS ONE

Article Title: Post-Transcriptional Regulation of Connexin43 in H-Ras-Transformed Cells

doi: 10.1371/journal.pone.0058500

Figure Lengend Snippet: a) Localization of the different Cx43 3′UTR constructs used in this work. All constructs contain the SV40 promoter (SV40-P) and the Luciferase coding region in addition to the pGL3 polyadenylation signal after the 3′UTR segments (not shown in graphics). The position of a putative polyadenylation signal is indicated. The S1516 region is shown in light grey. b, c) Luciferase assay of the first set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. d, e) Luciferase assay of the second set of 3′UTR constructs used to transfect NIH3T3 Neo and NIH3T3 Ras cells respectively. The firefly luciferase activities were reported to the Renilla luciferase control values as explained in “ ”. The experiments were performed at least three times in quadruplicates (* p <0.05, reported to the pGL3-pr construct).

Article Snippet: The luciferase vectors pGL3 (basic promoterless vector containing firefly luciferase), pGL3-pr (bearing an SV40 promoter) and pRL-Null (promoterless vector containing Renilla reniformis luciferase) were purchased from Promega (Madison, WI).

Techniques: Construct, Luciferase

Luciferase assay in MCF7 cells transfected with different constructs including the pGL3 control, the pGL3-Pr, in addition to the full length 3′untranslated region (pGL3-Pr-3′UTR) or the S1516 regulatory element (pGL3-Pr-S1516). The experiments were performed at least three times in quadruplicates (* p <0.05).

Journal: PLoS ONE

Article Title: Post-Transcriptional Regulation of Connexin43 in H-Ras-Transformed Cells

doi: 10.1371/journal.pone.0058500

Figure Lengend Snippet: Luciferase assay in MCF7 cells transfected with different constructs including the pGL3 control, the pGL3-Pr, in addition to the full length 3′untranslated region (pGL3-Pr-3′UTR) or the S1516 regulatory element (pGL3-Pr-S1516). The experiments were performed at least three times in quadruplicates (* p <0.05).

Article Snippet: The luciferase vectors pGL3 (basic promoterless vector containing firefly luciferase), pGL3-pr (bearing an SV40 promoter) and pRL-Null (promoterless vector containing Renilla reniformis luciferase) were purchased from Promega (Madison, WI).

Techniques: Luciferase, Transfection, Construct