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one step primescripttm rt pcr kit  (TaKaRa)


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    Structured Review

    TaKaRa one step primescripttm rt pcr kit
    One Step Primescripttm Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 2982 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+pcr+kit/One+Step+PrimeScript+RT-PCR+Kit/pmc13128697-57-7-15
    Average 97 stars, based on 2982 article reviews
    one step primescripttm rt pcr kit - by Bioz Stars, 2026-10
    97/100 stars

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    Reverse Transcription:

    Article Title: Mapping a high-risk progression-associated epithelial state in laryngeal dysplasia at single-cell resolution identifies PLK1 as a potential therapeutic target
    Article Snippet: RNA was extracted from the cell lines via the TRIzol method (Invitrogen, California, USA). .. Total RNA was reverse transcribed into cDNA via a One Step PrimeScript RT‒PCR Kit (TaKaRa, Japan). .. The target gene sequences were purchased from Boshang Biotechnology (Shanghai, China).

    Article Title: Intranasal gold nanoparticle-antigen induces mucosal and systemic immunity against β-coronaviruses
    Article Snippet: Trizol-based isolation methodology (Takara Bio Inc., Shiga, Japan) was employed for total RNA purification from homogenized specimens. .. Subsequent virological assessments involved quantitative reverse transcription PCR analyses using the One Step PrimeScript� RT-PCR system (Perfect Real Time) (Takara, Shiga, Japan) for nucleic acid quantification, following standardized laboratory protocols. ..

    Article Title: An emerging human eye disease is associated with aquatic virus zoonotic infection.
    Article Snippet: Climate change and human activities have increased the risk of virus spillover from wildlife, posing a threat to human health.. A human ocular disease called persistent ocular hypertensive viral anterior uveitis (POH-VAU) has recently emerged; however, the cause is unclear.. Here we report that POH-VAU is associated with covert mortality nodavirus (CMNV) of aquatic origin.

    Article Title: Time-dependent synergistic pathogenicity of sequential avian reovirus and Mycoplasma synoviae co-infection in SPF chickens.
    Article Snippet: Avian reovirus (ARV) and Mycoplasma synoviae (MS) are major pathogens associated with tarsal synovitis and growth suppression in broilers.. Yet the effect of infection order on their combined virulence remains poorly understood.. A survey of 202 swollen joint samples from northern Chinese farms (2024–2025) revealed 35.1% ARV–MS co-detection, identifying this dual infection as a frequent co-infection in broiler arthritis.

    Amplification:

    Article Title: OM-sqPCR enables one-step multiplex quantification of circulating small-RNA subclasses for colorectal cancer classification
    Article Snippet: .. To identify a reaction chemistry compatible with short-RNA amplification, four commercially available one-step RT-PCR kits—originally designed for long RNA or DNA templates—were evaluated following manufacturer protocols: (i) One Step PrimeScript RT-PCR Kit (TaKaRa, Japan, Cat. no. RC801S), (ii) one-step RT-PCR Kit (Novoprotein, China, Cat. no. E295), (iii) HiScript II U+ One Step qRT-PCR Probe Kit (Vazyme, China, Cat. no. P612-01), (iv) HotStart HiTag & Super M-MuLV one-step qRT-PCR Mix (Fapon, China, Cat. no. MD027). ..

    Article Title: Isolation and Transcriptomic Characterization of Genotype 5 Japanese Encephalitis Virus E138K Mutant Strain
    Article Snippet: .. PCR amplification was performed using the PrimeScript II High Fidelity One‐Step RT‐PCR Kit (Takara, Osaka, Japan). ..

    One Step RT-PCR:

    Article Title: OM-sqPCR enables one-step multiplex quantification of circulating small-RNA subclasses for colorectal cancer classification
    Article Snippet: .. To identify a reaction chemistry compatible with short-RNA amplification, four commercially available one-step RT-PCR kits—originally designed for long RNA or DNA templates—were evaluated following manufacturer protocols: (i) One Step PrimeScript RT-PCR Kit (TaKaRa, Japan, Cat. no. RC801S), (ii) one-step RT-PCR Kit (Novoprotein, China, Cat. no. E295), (iii) HiScript II U+ One Step qRT-PCR Probe Kit (Vazyme, China, Cat. no. P612-01), (iv) HotStart HiTag & Super M-MuLV one-step qRT-PCR Mix (Fapon, China, Cat. no. MD027). ..

    Article Title: Isolation and Transcriptomic Characterization of Genotype 5 Japanese Encephalitis Virus E138K Mutant Strain
    Article Snippet: .. PCR amplification was performed using the PrimeScript II High Fidelity One‐Step RT‐PCR Kit (Takara, Osaka, Japan). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: OM-sqPCR enables one-step multiplex quantification of circulating small-RNA subclasses for colorectal cancer classification
    Article Snippet: .. To identify a reaction chemistry compatible with short-RNA amplification, four commercially available one-step RT-PCR kits—originally designed for long RNA or DNA templates—were evaluated following manufacturer protocols: (i) One Step PrimeScript RT-PCR Kit (TaKaRa, Japan, Cat. no. RC801S), (ii) one-step RT-PCR Kit (Novoprotein, China, Cat. no. E295), (iii) HiScript II U+ One Step qRT-PCR Probe Kit (Vazyme, China, Cat. no. P612-01), (iv) HotStart HiTag & Super M-MuLV one-step qRT-PCR Mix (Fapon, China, Cat. no. MD027). ..

    Article Title: Intranasal gold nanoparticle-antigen induces mucosal and systemic immunity against β-coronaviruses
    Article Snippet: Trizol-based isolation methodology (Takara Bio Inc., Shiga, Japan) was employed for total RNA purification from homogenized specimens. .. Subsequent virological assessments involved quantitative reverse transcription PCR analyses using the One Step PrimeScript� RT-PCR system (Perfect Real Time) (Takara, Shiga, Japan) for nucleic acid quantification, following standardized laboratory protocols. ..

    Article Title: An emerging human eye disease is associated with aquatic virus zoonotic infection.
    Article Snippet: Climate change and human activities have increased the risk of virus spillover from wildlife, posing a threat to human health.. A human ocular disease called persistent ocular hypertensive viral anterior uveitis (POH-VAU) has recently emerged; however, the cause is unclear.. Here we report that POH-VAU is associated with covert mortality nodavirus (CMNV) of aquatic origin.

    Article Title: Time-dependent synergistic pathogenicity of sequential avian reovirus and Mycoplasma synoviae co-infection in SPF chickens.
    Article Snippet: Avian reovirus (ARV) and Mycoplasma synoviae (MS) are major pathogens associated with tarsal synovitis and growth suppression in broilers.. Yet the effect of infection order on their combined virulence remains poorly understood.. A survey of 202 swollen joint samples from northern Chinese farms (2024–2025) revealed 35.1% ARV–MS co-detection, identifying this dual infection as a frequent co-infection in broiler arthritis.

    Quantitative RT-PCR:

    Article Title: OM-sqPCR enables one-step multiplex quantification of circulating small-RNA subclasses for colorectal cancer classification
    Article Snippet: .. To identify a reaction chemistry compatible with short-RNA amplification, four commercially available one-step RT-PCR kits—originally designed for long RNA or DNA templates—were evaluated following manufacturer protocols: (i) One Step PrimeScript RT-PCR Kit (TaKaRa, Japan, Cat. no. RC801S), (ii) one-step RT-PCR Kit (Novoprotein, China, Cat. no. E295), (iii) HiScript II U+ One Step qRT-PCR Probe Kit (Vazyme, China, Cat. no. P612-01), (iv) HotStart HiTag & Super M-MuLV one-step qRT-PCR Mix (Fapon, China, Cat. no. MD027). ..

    Article Title: Sphingobacterium-mediated EPS shielding exacerbates norovirus environmental persistence and risk in swine farms.
    Article Snippet: Norovirus (NoV) is a major cause of epidemic acute gastroenteritis worldwide, and contaminated food and water are important routes of NoV transmission.. However, the environmental fate of NoV and the factors contributing to its persistence remain poorly understood.. This study investigated NoV occurrence in small-scale swine farm compost and intensive swine wastewater treatment systems (WWTSs), and explored the potential bacterial factors associated with viral persistence.

    Article Title: Time-dependent synergistic pathogenicity of sequential avian reovirus and Mycoplasma synoviae co-infection in SPF chickens.
    Article Snippet: Avian reovirus (ARV) and Mycoplasma synoviae (MS) are major pathogens associated with tarsal synovitis and growth suppression in broilers.. Yet the effect of infection order on their combined virulence remains poorly understood.. A survey of 202 swollen joint samples from northern Chinese farms (2024–2025) revealed 35.1% ARV–MS co-detection, identifying this dual infection as a frequent co-infection in broiler arthritis.

    Polymerase Chain Reaction:

    Article Title: Intranasal gold nanoparticle-antigen induces mucosal and systemic immunity against β-coronaviruses
    Article Snippet: Trizol-based isolation methodology (Takara Bio Inc., Shiga, Japan) was employed for total RNA purification from homogenized specimens. .. Subsequent virological assessments involved quantitative reverse transcription PCR analyses using the One Step PrimeScript� RT-PCR system (Perfect Real Time) (Takara, Shiga, Japan) for nucleic acid quantification, following standardized laboratory protocols. ..

    Article Title: Isolation and Transcriptomic Characterization of Genotype 5 Japanese Encephalitis Virus E138K Mutant Strain
    Article Snippet: .. PCR amplification was performed using the PrimeScript II High Fidelity One‐Step RT‐PCR Kit (Takara, Osaka, Japan). ..

    other:

    Article Title: Carboplatin alleviates astrocytic TDP-43 neurotoxicity by inhibiting NF-κB activation.
    Article Snippet: Amyotrophic lateral sclerosis (ALS) is a rare and progressive motor neuron disease; however, its exact pathogenic mechanisms remain unclear.. Currently, no effective treatments are available for this disease.. Therefore, in this study, we investigated the anti-inflammatory effects of the anti-cancer agent, carboplatin, on neuronal cells and its potential therapeutic effects against ALS.

    Real-time Polymerase Chain Reaction:

    Article Title: An emerging human eye disease is associated with aquatic virus zoonotic infection.
    Article Snippet: Climate change and human activities have increased the risk of virus spillover from wildlife, posing a threat to human health.. A human ocular disease called persistent ocular hypertensive viral anterior uveitis (POH-VAU) has recently emerged; however, the cause is unclear.. Here we report that POH-VAU is associated with covert mortality nodavirus (CMNV) of aquatic origin.



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    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
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    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
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    Image Search Results


    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Journal: Veterinary Research

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves

    doi: 10.1186/s13567-026-01765-3

    Figure Lengend Snippet: Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Article Snippet: The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction.

    Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification, Marker, Generated, Clone Assay, Construct, Recombinant, Negative Control, Control