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one step primescripttm rt pcr kit  (TaKaRa)


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    Structured Review

    TaKaRa one step primescripttm rt pcr kit
    One Step Primescripttm Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 2965 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+pcr+kit/One+Step+PrimeScript+RT-PCR+Kit/pmc13128697-57-7-15
    Average 97 stars, based on 2965 article reviews
    one step primescripttm rt pcr kit - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: FeiyanHeji multifunctionally inhibits influenza virus via PA protein degradation and RIG-I signaling pathway potentiation.
    Article Snippet: .. RNA levels were measured using the One Step PrimeScript RT-PCR Kit (RR064B, Takara) on a CFX96 real-time PCR system (Bio-Rad). ..

    Article Title: Carboplatin alleviates astrocytic TDP-43 neurotoxicity by inhibiting NF-κB activation.
    Article Snippet: Amyotrophic lateral sclerosis (ALS) is a rare and progressive motor neuron disease; however, its exact pathogenic mechanisms remain unclear.. Currently, no effective treatments are available for this disease.. Therefore, in this study, we investigated the anti-inflammatory effects of the anti-cancer agent, carboplatin, on neuronal cells and its potential therapeutic effects against ALS.

    Article Title: Evaluating Viral Pollution in Wastewater and Mediterranean Ecosystems
    Article Snippet: .. Viral detection of process control viruses, PEDV and MgV, was performed by reverse transcription qPCR (RT-qPCR) using the One Step PrimeScriptTM RT-PCR Kit (Perfect Real Time, Takara Bio Inc., USA). .. Detection of norovirus (HuNoV) genogroups I and II, human astrovirus (HAstV), rotavirus (RV), hepatitis A virus (HAV), and hepatitis E virus (HEV) were carried out using the RNA UltraSense One-Step kit (Invitrogen, USA).

    Article Title: FeiyanHeji multifunctionally inhibits influenza virus via PA protein degradation and RIG-I signaling pathway potentiation
    Article Snippet: .. RNA levels were measured using the One Step PrimeScript RT-PCR Kit (RR064B, Takara) on a CFX96 real-time PCR system (Bio-Rad). ..

    Article Title: Evaluating Viral Pollution in Wastewater and Mediterranean Ecosystems
    Article Snippet: Pepper mild mottle virus (PMMoV) was quantified with the ‘PMMoV Faecal Indicator RT-qPCR Kit’ (Promega), according to the manufacturer’s recommendations. .. Detection of SARS-CoV-2 was performed using the One Step PrimeScriptTM RT-PCR Kit (Perfect Real Time, Takara Bio Inc.) and targeting the N1 region of the nucleocapsid gene (CDC, ). ..

    Article Title: Marburg Virus Glycoprotein Is a Remarkable Virulent Factor Linked to Hemorrhagic Pathology: Evidence from Multimodal Experimental Systems.
    Article Snippet: .. One-step qRT-PCR was then performed using the One-Step PrimeScript RT-PCR Kit (#RR064A, Takara) and a StepOnePlus Real-Time PCR System. ..

    Real-time Polymerase Chain Reaction:

    Article Title: FeiyanHeji multifunctionally inhibits influenza virus via PA protein degradation and RIG-I signaling pathway potentiation.
    Article Snippet: .. RNA levels were measured using the One Step PrimeScript RT-PCR Kit (RR064B, Takara) on a CFX96 real-time PCR system (Bio-Rad). ..

    Article Title: Carboplatin alleviates astrocytic TDP-43 neurotoxicity by inhibiting NF-κB activation.
    Article Snippet: Amyotrophic lateral sclerosis (ALS) is a rare and progressive motor neuron disease; however, its exact pathogenic mechanisms remain unclear.. Currently, no effective treatments are available for this disease.. Therefore, in this study, we investigated the anti-inflammatory effects of the anti-cancer agent, carboplatin, on neuronal cells and its potential therapeutic effects against ALS.

    Article Title: Evaluating Viral Pollution in Wastewater and Mediterranean Ecosystems
    Article Snippet: .. Viral detection of process control viruses, PEDV and MgV, was performed by reverse transcription qPCR (RT-qPCR) using the One Step PrimeScriptTM RT-PCR Kit (Perfect Real Time, Takara Bio Inc., USA). .. Detection of norovirus (HuNoV) genogroups I and II, human astrovirus (HAstV), rotavirus (RV), hepatitis A virus (HAV), and hepatitis E virus (HEV) were carried out using the RNA UltraSense One-Step kit (Invitrogen, USA).

    Article Title: FeiyanHeji multifunctionally inhibits influenza virus via PA protein degradation and RIG-I signaling pathway potentiation
    Article Snippet: .. RNA levels were measured using the One Step PrimeScript RT-PCR Kit (RR064B, Takara) on a CFX96 real-time PCR system (Bio-Rad). ..

    Article Title: Marburg Virus Glycoprotein Is a Remarkable Virulent Factor Linked to Hemorrhagic Pathology: Evidence from Multimodal Experimental Systems.
    Article Snippet: .. One-step qRT-PCR was then performed using the One-Step PrimeScript RT-PCR Kit (#RR064A, Takara) and a StepOnePlus Real-Time PCR System. ..

    Polymerase Chain Reaction:

    Article Title: Isolation and Transcriptomic Characterization of Genotype 5 Japanese Encephalitis Virus E138K Mutant Strain
    Article Snippet: .. PCR amplification was performed using the PrimeScript II High Fidelity One‐Step RT‐PCR Kit (Takara, Osaka, Japan). ..

    Amplification:

    Article Title: Isolation and Transcriptomic Characterization of Genotype 5 Japanese Encephalitis Virus E138K Mutant Strain
    Article Snippet: .. PCR amplification was performed using the PrimeScript II High Fidelity One‐Step RT‐PCR Kit (Takara, Osaka, Japan). ..

    One Step RT-PCR:

    Article Title: Isolation and Transcriptomic Characterization of Genotype 5 Japanese Encephalitis Virus E138K Mutant Strain
    Article Snippet: .. PCR amplification was performed using the PrimeScript II High Fidelity One‐Step RT‐PCR Kit (Takara, Osaka, Japan). ..

    Article Title: Chikungunya virus in dengue-suspected patients: Molecular evidence from the 2019 outbreak in Yangon, Myanmar.
    Article Snippet: .. To confirm viral isolation, RNA extracted from the ICF was analyzed by conventional one-step RT-PCR using the Primescript One-Step RT-PCR Kit (Takara Bio Inc., Shiga, Japan) with established primers, and protocols as previously described [5,19]. ..

    Isolation:

    Article Title: Chikungunya virus in dengue-suspected patients: Molecular evidence from the 2019 outbreak in Yangon, Myanmar.
    Article Snippet: .. To confirm viral isolation, RNA extracted from the ICF was analyzed by conventional one-step RT-PCR using the Primescript One-Step RT-PCR Kit (Takara Bio Inc., Shiga, Japan) with established primers, and protocols as previously described [5,19]. ..

    Control:

    Article Title: Evaluating Viral Pollution in Wastewater and Mediterranean Ecosystems
    Article Snippet: .. Viral detection of process control viruses, PEDV and MgV, was performed by reverse transcription qPCR (RT-qPCR) using the One Step PrimeScriptTM RT-PCR Kit (Perfect Real Time, Takara Bio Inc., USA). .. Detection of norovirus (HuNoV) genogroups I and II, human astrovirus (HAstV), rotavirus (RV), hepatitis A virus (HAV), and hepatitis E virus (HEV) were carried out using the RNA UltraSense One-Step kit (Invitrogen, USA).

    Reverse Transcription:

    Article Title: Evaluating Viral Pollution in Wastewater and Mediterranean Ecosystems
    Article Snippet: .. Viral detection of process control viruses, PEDV and MgV, was performed by reverse transcription qPCR (RT-qPCR) using the One Step PrimeScriptTM RT-PCR Kit (Perfect Real Time, Takara Bio Inc., USA). .. Detection of norovirus (HuNoV) genogroups I and II, human astrovirus (HAstV), rotavirus (RV), hepatitis A virus (HAV), and hepatitis E virus (HEV) were carried out using the RNA UltraSense One-Step kit (Invitrogen, USA).

    Quantitative RT-PCR:

    Article Title: Marburg Virus Glycoprotein Is a Remarkable Virulent Factor Linked to Hemorrhagic Pathology: Evidence from Multimodal Experimental Systems.
    Article Snippet: .. One-step qRT-PCR was then performed using the One-Step PrimeScript RT-PCR Kit (#RR064A, Takara) and a StepOnePlus Real-Time PCR System. ..



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    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
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    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
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    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
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    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
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    TaKaRa primescripttm ii high fidelity rt pcr kit
    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
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    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template <t>for</t> <t>RT-PCR</t> to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.
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    Image Search Results


    Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Journal: Veterinary Research

    Article Title: Potent neutralization and therapeutic efficacy of bovine rotavirus-specific VHH antibodies in infected calves

    doi: 10.1186/s13567-026-01765-3

    Figure Lengend Snippet: Construction and screening of phage display library . A PBMCs were isolated from camels, and RNA was extracted as a template for RT-PCR to amplify the gene sequence containing the leader signal sequence before the CH2 region. The amplification yielded two fragments: a 900-bp fragment (VH–CH1–CH2) and a 600-bp fragment (VHH–CH2). Lane M represents the DNA marker (2000–100 bp), and lanes 1–4 represent the amplified fragments. B The 600-bp fragment obtained from the first round of PCR was used as a template for the second round of PCR, which successfully generated the full-length VHH gene (spanning FR1 to FR4) with an approximate size of 400 bp. Lane M represents the DNA marker (1000–200 bp), and lanes 1–4 represent the amplified fragments. C A total of 16 clones were randomly selected from the constructed phage antibody library for identification. D Overall, 92 recombinant phage clones were randomly selected from the third round of panning and added to microplates coated with BRV. HRP-conjugated anti-M13 monoclonal antibody was used to detect the bound phages. M13K07 helper phage and PBS served as the negative control and blank control, respectively. Positive clones were defined as those with a sample-to-negative control ratio (P/N) ≥ 2.1, i.e., clones above the reference line were identified as positive.

    Article Snippet: The extracted RNA was directly subjected to one-step reverse-transcription polymerase chain reaction (RT-PCR) amplification using the PrimeScriptTM One Step RT-PCR Kit (TaKaRa, Shiga, Japan), which allows reverse transcription and PCR amplification to be conducted in a single reaction.

    Techniques: Isolation, Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification, Marker, Generated, Clone Assay, Construct, Recombinant, Negative Control, Control