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primescript rt reagent kit  (BGI Genomics Co)

 
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    BGI Genomics Co primescript rt reagent kit
    Primescript Rt Reagent Kit, supplied by BGI Genomics Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+master+mix+kit/primescript+rt+reagent+kit/pmc11986740-80-16-20
    Average 90 stars, based on 1 article reviews
    primescript rt reagent kit - by Bioz Stars, 2026-11
    90/100 stars

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    Isolation:

    Article Title: The synergistic effects of PM 2.5 and high-fat diet on Th1/Th2 balance in model mice with asthma
    Article Snippet: .. From the isolated RNA, 2 μg was used to synthesize first-strand complementary DNA (cDNA) using a Primescript RT Reagent Kit (BGI Genomics Co., Ltd., Shenzhen, China) as per the manufacturer’s protocol. .. RT-PCR was performed using a SYBR Green Mix kit (Takara Bio, Otsu, Japan).



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    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

    Journal: Frontiers in Neurology

    Article Title: Study on Leigh syndrome caused by SURF1 gene mutations and its mechanisms

    doi: 10.3389/fneur.2026.1793054

    Figure Lengend Snippet: Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

    Article Snippet: Total RNA was extracted from patients and their parents using the Qiagen RNA Preparation Kit, and complementary DNA (cDNA) was synthesized by reverse transcription using the PrimeScriptTM Strand cDNA Synthesis Kit/RT Master Mix (TAKARA).

    Techniques: Expressing, Variant Assay, Amplification