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primescripttm rt kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm rt kit
    Primescripttm Rt Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26928 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+rt+master+mix+kit/PrimeScript+RT+Master+Mix/pm41985718-65-13-17
    Average 99 stars, based on 26928 article reviews
    primescripttm rt kit - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: TXNRD1-driven enteric glial remodeling promotes neuronal dysfunction in Hirschsprung disease
    Article Snippet: Total RNA was extracted from tissues or cells using TRIzol reagent (Invitrogen, USA). .. Reverse transcription was performed using PrimeScript RT Master Mix (RR036Q, Takara, Japan) to synthesize cDNA. .. Next, the cDNA fragments were amplified and analyzed on an ABI 7500 PCR system (ThermoFisher Scientific, USA).

    Article Title: Ac NR1F orchestrates epidermal remodeling to enable host adaptation in Angiostrongylus cantonensis
    Article Snippet: Worm morphology was observed using a stereomicroscopy (Leica, Germany), and the size of eggs was calculated using Image J. .. Total RNA was extracted from adult worms using Trizol (Invitrogen, USA) and reverse transcribed into cDNA using the PrimeScript RT Master Mix (TaKaRa, Japan). .. A. cantonensis nr1f gene was amplified using Phanta Max Super-Fidelity DNA Polymerase (Vazyme, China) and cloned into pMD19-T and transformed in Escherichia coli DH5α.

    Article Title: USP8 promotes ferroptosis-associated senescence-like phenotype in chicken ovarian follicular granulosa cells via ACSL4.
    Article Snippet: Ferroptosis is an iron-dependent form of cell death triggered by intracellular lipid peroxidation.. It has been well documented to participate in multiple mammalian ovarian diseases; however, its functional significance in avian reproduction remains largely unclear.. Here, we explored the role of the deubiquitinating enzyme ubiquitinspecific protease 8 (USP8) in governing ferroptosis and subsequent cellular senescence in chicken follicular granulosa cells.

    Article Title: D-allose reprogrammes the mitophagy-mtDNA-cGAS-STING-ADM2 axis to restore nucleus pulposus cell homeostasis in intervertebral disc degeneration.
    Article Snippet: RNA concentration and purity were assessed with a NanoDrop spectrophotometer (Thermo Experimental & Molecular Medicine Fisher Scientific, USA). .. The reverse transcription reaction was performed using PrimeScript RT Master Mix (Takara Bio, China). .. Real-time reverse transcription quantitative PCR (RT-qPCR) was performed with TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio, China) on a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific, USA).

    Generated:

    Article Title: ATG5-overexpressing MSCs improve ligament healing with an associated shift toward M2 macrophage polarization in aged rats
    Article Snippet: Total RNA was purified using TRIzol reagent (Invitrogen, USA) following the manufacturer’s protocol. .. Complementary DNA (cDNA) was generated from equal amounts of RNA using the PrimeScript RT Master Mix kit (Takara, Japan). .. Quantitative RT-PCR was then performed with TB Green Premix Ex Taq II (Takara, Japan) on a LightCycler 480 instrument (Roche, Switzerland).

    cDNA Synthesis:

    Article Title: USP8 promotes ferroptosis-associated senescence-like phenotype in chicken ovarian follicular granulosa cells via ACSL4.
    Article Snippet: Ferroptosis is an iron-dependent form of cell death triggered by intracellular lipid peroxidation.. It has been well documented to participate in multiple mammalian ovarian diseases; however, its functional significance in avian reproduction remains largely unclear.. Here, we explored the role of the deubiquitinating enzyme ubiquitinspecific protease 8 (USP8) in governing ferroptosis and subsequent cellular senescence in chicken follicular granulosa cells.



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    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of <t>cDNA</t> from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.
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    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of <t>cDNA</t> from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.
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    Image Search Results


    Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

    Journal: Frontiers in Neurology

    Article Title: Study on Leigh syndrome caused by SURF1 gene mutations and its mechanisms

    doi: 10.3389/fneur.2026.1793054

    Figure Lengend Snippet: Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

    Article Snippet: Total RNA was extracted from patients and their parents using the Qiagen RNA Preparation Kit, and complementary DNA (cDNA) was synthesized by reverse transcription using the PrimeScriptTM Strand cDNA Synthesis Kit/RT Master Mix (TAKARA).

    Techniques: Expressing, Variant Assay, Amplification