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Beijing CWBio primescript rt master mix
Primescript Rt Master Mix, supplied by Beijing CWBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primescripttm+rt+master+mix+kit/primescript+rt+master+mix/pm38697105-305-25-17
Average 90 stars, based on 1 article reviews
primescript rt master mix - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Synthesized:

Article Title: Next-generation anti-PD-L1/IL-15 immunocytokine elicits superior antitumor immunity in cold tumors with minimal toxicity
Article Snippet: .. Total RNAs were extracted from tissues using the Ultrapure RNA Kit (Cwbio, China), and cDNA was synthesized using a PrimeScript RT Master Mix. .. Real-time qRT-PCR was performed on an Applied Biosystems 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, USA) using Hieff qPCR SYBR Green Master Mix.

Article Title: Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells.
Article Snippet: • The main plasticizer compounds that migrated from fast food packaging materials into oily foods were DEHP, DBP and DEHA.. • 0.25 ~ 25 μM these chemicals could significantly increase the E2 level and reduce the T level in H295R cells.. • The potential endocrine-disrupting effects of phthalates and adipates were triggered by steroidogenesis pathways.

Reverse Transcription:

Article Title: Development of astaxanthin-loaded layer-by-layer emulsions: physicochemical properties and improvement of LPS-induced neuroinflammation in mice
Article Snippet: Astaxanthin (AST) has been shown to have neuroprotective effects; however, its bioavailability in vivo is low due to its hydrophobic properties.. In this study, lactoferrin (LF) was prepared by heat-treatment at different temperatures, and on this basis, a layer-by-layer self-assembly method was used to construct double-layer emulsions with LF as the inner layer and polysaccharide (beet pectin, BP or carboxymethyl chitosan, CMCS) as the outer layer.. Then AST was encapsulated in the emulsions and their physiochemical properties and function were investigated.

Polymerase Chain Reaction:

Article Title: Clinicopathological Significance of Decreased Expression of the Tumor Inhibitor Gene PDCD5 in Osteoclastoma.
Article Snippet: Background: The gene programmed cell death 5 (PDCD5) has recently been characterized as a tumor suppressor gene and is believed to be an important prognostic cancer marker; it is frequently involved in neoplastic transformation and apoptosis of tumor cells.. Several studies have demonstrated a decrease or loss of expression of PDCD5 in certain tumors.. However, the relevance of PDCD5 expression in human osteoclastoma and its clinicopathological significance have not been extensively studied.

AST Assay:

Article Title: Development of astaxanthin-loaded layer-by-layer emulsions: physicochemical properties and improvement of LPS-induced neuroinflammation in mice
Article Snippet: Astaxanthin (AST) has been shown to have neuroprotective effects; however, its bioavailability in vivo is low due to its hydrophobic properties.. In this study, lactoferrin (LF) was prepared by heat-treatment at different temperatures, and on this basis, a layer-by-layer self-assembly method was used to construct double-layer emulsions with LF as the inner layer and polysaccharide (beet pectin, BP or carboxymethyl chitosan, CMCS) as the outer layer.. Then AST was encapsulated in the emulsions and their physiochemical properties and function were investigated.

Protein Extraction:

Article Title: Development of astaxanthin-loaded layer-by-layer emulsions: physicochemical properties and improvement of LPS-induced neuroinflammation in mice
Article Snippet: Astaxanthin (AST) has been shown to have neuroprotective effects; however, its bioavailability in vivo is low due to its hydrophobic properties.. In this study, lactoferrin (LF) was prepared by heat-treatment at different temperatures, and on this basis, a layer-by-layer self-assembly method was used to construct double-layer emulsions with LF as the inner layer and polysaccharide (beet pectin, BP or carboxymethyl chitosan, CMCS) as the outer layer.. Then AST was encapsulated in the emulsions and their physiochemical properties and function were investigated.

Bicinchoninic Acid Protein Assay:

Article Title: Development of astaxanthin-loaded layer-by-layer emulsions: physicochemical properties and improvement of LPS-induced neuroinflammation in mice
Article Snippet: Astaxanthin (AST) has been shown to have neuroprotective effects; however, its bioavailability in vivo is low due to its hydrophobic properties.. In this study, lactoferrin (LF) was prepared by heat-treatment at different temperatures, and on this basis, a layer-by-layer self-assembly method was used to construct double-layer emulsions with LF as the inner layer and polysaccharide (beet pectin, BP or carboxymethyl chitosan, CMCS) as the outer layer.. Then AST was encapsulated in the emulsions and their physiochemical properties and function were investigated.

Western Blot:

Article Title: Development of astaxanthin-loaded layer-by-layer emulsions: physicochemical properties and improvement of LPS-induced neuroinflammation in mice
Article Snippet: Astaxanthin (AST) has been shown to have neuroprotective effects; however, its bioavailability in vivo is low due to its hydrophobic properties.. In this study, lactoferrin (LF) was prepared by heat-treatment at different temperatures, and on this basis, a layer-by-layer self-assembly method was used to construct double-layer emulsions with LF as the inner layer and polysaccharide (beet pectin, BP or carboxymethyl chitosan, CMCS) as the outer layer.. Then AST was encapsulated in the emulsions and their physiochemical properties and function were investigated.

Isolation:

Article Title: Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells.
Article Snippet: • The main plasticizer compounds that migrated from fast food packaging materials into oily foods were DEHP, DBP and DEHA.. • 0.25 ~ 25 μM these chemicals could significantly increase the E2 level and reduce the T level in H295R cells.. • The potential endocrine-disrupting effects of phthalates and adipates were triggered by steroidogenesis pathways.

RNA Extraction:

Article Title: Effects of fast food packaging plasticizers and their metabolites on steroid hormone synthesis in H295R cells.
Article Snippet: • The main plasticizer compounds that migrated from fast food packaging materials into oily foods were DEHP, DBP and DEHA.. • 0.25 ~ 25 μM these chemicals could significantly increase the E2 level and reduce the T level in H295R cells.. • The potential endocrine-disrupting effects of phthalates and adipates were triggered by steroidogenesis pathways.

other:

Article Title: Next-generation anti-PD-L1/IL-15 immunocytokine elicits superior antitumor immunity in cold tumors with minimal toxicity.
Article Snippet: Real-time qRT-PCR was performed on an Applied Biosystems 7500 Fast Real-Time PCR System (Thermo Fisher Scientific, USA) using Hieff qPCR SYBR Green Master Mix.



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Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of <t>cDNA</t> from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.
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Image Search Results


Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

Journal: Frontiers in Neurology

Article Title: Study on Leigh syndrome caused by SURF1 gene mutations and its mechanisms

doi: 10.3389/fneur.2026.1793054

Figure Lengend Snippet: Genetic analysis and expression of SURF1 gene variants in Case 2. (A) The probands 2 (P2) intronic variant c.515 + 3G > C caused abnormal splicing of the SURF1 gene, leading to the deletion of exon 5. (B) The expression level of the SURF1 gene in probands 2 (P2) was significantly lower than those in their parents. (C) PCR results of cDNA from the nuclear family of probands 2 (P2), the amplicon in the proband was shorter than the corresponding bands in the parents.

Article Snippet: Total RNA was extracted from patients and their parents using the Qiagen RNA Preparation Kit, and complementary DNA (cDNA) was synthesized by reverse transcription using the PrimeScriptTM Strand cDNA Synthesis Kit/RT Master Mix (TAKARA).

Techniques: Expressing, Variant Assay, Amplification