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primescripttm reverse transcriptase  (TaKaRa)


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    TaKaRa primescripttm reverse transcriptase
    Primescripttm Reverse Transcriptase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 87884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+reverse+transcriptase+reagent+kit/PrimeScript+RT+Reagent+Kit/pm41928286-487-13-16
    Average 99 stars, based on 87884 article reviews
    primescripttm reverse transcriptase - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: Local periodontal injection, systemic heart repair: A dual-functional hydrogel for non-invasive concurrent treatment of periodontitis and myocardial infarction
    Article Snippet: .. The isolated RNA was then reverse transcribed into cDNA using PrimeScriptTM RT reagent kit (Takara), and the expression of osteogenic differentiation-related genes, including osteocalcin (OCN), alkaline phosphatase (ALP), and collagen type I (COL-1) were quantified by real-time PCR (RT‒qPCR). ..

    Reverse Transcription:

    Article Title: Local periodontal injection, systemic heart repair: A dual-functional hydrogel for non-invasive concurrent treatment of periodontitis and myocardial infarction
    Article Snippet: .. The isolated RNA was then reverse transcribed into cDNA using PrimeScriptTM RT reagent kit (Takara), and the expression of osteogenic differentiation-related genes, including osteocalcin (OCN), alkaline phosphatase (ALP), and collagen type I (COL-1) were quantified by real-time PCR (RT‒qPCR). ..

    Article Title: Smart microenvironment-adaptive nanocatalytic hydrogel for sequential antibacterial, anti-inflammatory, and regenerative therapy of biofilm-infected wounds
    Article Snippet: Total RNA was extracted using the RNeasy kit (Qiagen) according to the manufacturer's instructions. .. Then, 1 μg of total RNA was reverse-transcribed into cDNA using the PrimeScript RT Reagent Kit (Takara). .. Quantitative PCR was performed in triplicate for each sample on an ABI Prism 7900 HT system (Applied Biosystems, USA) using SYBR Green reagent (Takara), with 10 ng of cDNA as the template.

    Article Title: In vitro and in vivo anti-piroplasm activity of PBT2 against Theileria annulata and Babesia microti
    Article Snippet: Total RNA was extracted using the RNeasy Mini Kit (Qiagen, Germany) according to the manufacturer's instructions. .. PrimeScriptTM RT Reagent Kit (TaKaRa, Japan) was used for reverse transcribed of 1 μg RNA. ..

    Article Title: Chiral Fe 3 O 4 /GelMA hydrogels regulate the osteoimmune microenvironment via Itgb3-mediated macrophage polarization to combat peri-implantitis
    Article Snippet: The reverse transcription step was performed using a PCR thermocycler (Takara). .. The mRNA was reverse transcribed into cDNA using a PrimeScriptTM RT kit (Takara Bio, Otsu, Japan). cDNA samples were amplified by qRT-PCR according to the primer sequences listed below and normalized to the housekeeping gene β-actin. ..

    Article Title: A dual-functional engineered exosome-laden hydrogel redirects endogenous neural stem cell fate for spinal cord injury repair
    Article Snippet: Finally the membrane was exposed for imaging using an enhanced chemiluminescence system (Bio-Rad, Hercules, CA, USA). .. Total RNA was extracted using a Total RNA Extraction Kit (R1200, Solarbio, Beijing, China). mRNA was reverse-transcribed into cDNA using the Primescript RT reagent Kit (RR037A, Takara, Tokyo, Japan). .. Subsequently, quantitative real-time PCR was performed using the LightCycler 96 system and TB Green Preix Ex Taq II (RR820B, Takara).

    Expressing:

    Article Title: Local periodontal injection, systemic heart repair: A dual-functional hydrogel for non-invasive concurrent treatment of periodontitis and myocardial infarction
    Article Snippet: .. The isolated RNA was then reverse transcribed into cDNA using PrimeScriptTM RT reagent kit (Takara), and the expression of osteogenic differentiation-related genes, including osteocalcin (OCN), alkaline phosphatase (ALP), and collagen type I (COL-1) were quantified by real-time PCR (RT‒qPCR). ..

    Real-time Polymerase Chain Reaction:

    Article Title: Local periodontal injection, systemic heart repair: A dual-functional hydrogel for non-invasive concurrent treatment of periodontitis and myocardial infarction
    Article Snippet: .. The isolated RNA was then reverse transcribed into cDNA using PrimeScriptTM RT reagent kit (Takara), and the expression of osteogenic differentiation-related genes, including osteocalcin (OCN), alkaline phosphatase (ALP), and collagen type I (COL-1) were quantified by real-time PCR (RT‒qPCR). ..

    Amplification:

    Article Title: Chiral Fe 3 O 4 /GelMA hydrogels regulate the osteoimmune microenvironment via Itgb3-mediated macrophage polarization to combat peri-implantitis
    Article Snippet: The reverse transcription step was performed using a PCR thermocycler (Takara). .. The mRNA was reverse transcribed into cDNA using a PrimeScriptTM RT kit (Takara Bio, Otsu, Japan). cDNA samples were amplified by qRT-PCR according to the primer sequences listed below and normalized to the housekeeping gene β-actin. ..

    Quantitative RT-PCR:

    Article Title: Chiral Fe 3 O 4 /GelMA hydrogels regulate the osteoimmune microenvironment via Itgb3-mediated macrophage polarization to combat peri-implantitis
    Article Snippet: The reverse transcription step was performed using a PCR thermocycler (Takara). .. The mRNA was reverse transcribed into cDNA using a PrimeScriptTM RT kit (Takara Bio, Otsu, Japan). cDNA samples were amplified by qRT-PCR according to the primer sequences listed below and normalized to the housekeeping gene β-actin. ..

    Synthesized:

    Article Title: Melatonin-incorporated brain extracellular matrix hydrogel enhances NSCs mitochondrial metabolism to promote neuroregeneration via the AMPK-PGC-1α-NRF1/TFAM axis after spinal cord injury
    Article Snippet: Total RNA was extracted from cells using the Eastep Super Total RNA Extraction Kit (LS1040, Shanghai Promega, China) according to the manufacturer's protocol. .. First-strand cDNA was then synthesized from the extracted RNA using the PrimeScript Fast RT Reagent Kit with gDNA Eraser (RR092A, Takara, Japan). .. Quantitative PCR was subsequently performed using TB Green Premix Ex Taq II Fast (CN830A, Takara, Japan) on a QuantStudio 5 real-time PCR system (Applied Biosystems, USA).

    RNA Extraction:

    Article Title: A dual-functional engineered exosome-laden hydrogel redirects endogenous neural stem cell fate for spinal cord injury repair
    Article Snippet: Finally the membrane was exposed for imaging using an enhanced chemiluminescence system (Bio-Rad, Hercules, CA, USA). .. Total RNA was extracted using a Total RNA Extraction Kit (R1200, Solarbio, Beijing, China). mRNA was reverse-transcribed into cDNA using the Primescript RT reagent Kit (RR037A, Takara, Tokyo, Japan). .. Subsequently, quantitative real-time PCR was performed using the LightCycler 96 system and TB Green Preix Ex Taq II (RR820B, Takara).



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