prime script rtmaster mix kit (TaKaRa)
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prime script rtmaster mix kit
Prime Script Rtmaster Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+rtmaster+mix+kit/PrimeScript+RT+Master+Mix/pm41238541-385-15-20
Average 99 stars, based on 26966 article reviews
Prime Script Rtmaster Mix Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26966 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+rtmaster+mix+kit/PrimeScript+RT+Master+Mix/pm41238541-385-15-20
Average 99 stars, based on 26966 article reviews
prime script rtmaster mix kit - by Bioz Stars,
2026-10
99/100 stars
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Article Snippet: Reagents and tools table Reagent or resource Source Identifier Reagent or resource Source Identifier Antibodies Anti-CD45, fluorophore-conjugated Thermo Fisher #11-0451-81 Anti-CD31, fluorophoreconjugated Thermo Fisher #11-0311-81 Anti-Ter119, fluorophore-conjugated Thermo Fisher #11-5921-82 Anti-CD49f, fluorophore-conjugated R&D Systems #FAB13501P Anti-CD24, fluorophore-conjugated BD Biosciences #562349 Anti-Keratin 14 (Krt14), used for RNA-FISH costaining FN (as written in manuscript) #FNab02195 Anti-alpha smooth muscle actin, used for RNA-FISH co-staining Proteintech #23081-1-AP Anti-p120 Catenin, used for RNA-FISH costaining Proteintech #66208-1-Ig Anti-Ki67, organoid immunofluorescence/ tissue IHC Abcam #ab15580 Anti-Krt8 Cohesionbio #CPA1659 Anti-Krt14, tissue immunofluorescence FineTest #FNab02195 Anti-Acta2, tissue immunofluorescence Proteintech #14395-1-AP Anti-p120 Catenin, tissue immunofluorescence Proteintech #66208-1-Ig Anti-TWIST1 Affinity/Affinity Biosciences #AF4009 Anti-CTND1 Bethyl Laboratories #A300-978A-T Anti-Flag CST #14793 Anti-HA CST #3724 Anti-beta-catenin, ChIP Abcam #ab32572 Secondary fluorescence antibodies Abcam; Signalway Antibody Goat anti-Rabbit 488, ab150077; Goat antiRabbit 594, L3017; Goat anti-Rabbit 647, © The Author(s) EMBO reports 11 Reagent or resource Source Identifier L35451; Goat anti-Rat 594, ab150160 Chemicals, peptides, recombinant proteins, and culture reagents Collagenase/ Hyaluronidase STEMCELL Technologies #07912 EpiCult-B supplement STEMCELL Technologies #05611 EpiCult-B basal medium STEMCELL Technologies #05610 NH4Cl (red blood cell removal) STEMCELL Technologies 07850 Trypsin-EDTA, 0.25% TransGen FG301-01 Dispase, MaSC dissociation STEMCELL Technologies #07913 DNase I STEMCELL Technologies #07900 Advanced DMEM/F-12 Gibco #12634010 Matrigel Corning Life Sciences/ Corning #354234 Epidermal growth factor (EGF) Sigma #E9644 FGF2, MaSC culture EMD Millipore #GF003 Heparin ExcellBio #FSP500 Y-27632 R&D Systems #1254 CHIR, MaSC culture InvivoChem #V0211 Dispase, lacrimal epithelial stem cell isolation (25 U/mL) STEMCELL Technologies #07913 Collagenase I Biosharp #bs163 N2 supplement Gibco #17502048 B27 supplement Gibco #17504044 GlutaMAX Gibco #35050061 FGF10 PeproTech #100-26 Wnt3a, lacrimal epithelial stem cell culture PeproTech #315-20 DMEM/F-12, salivary epithelial stem cell culture Gibco 12634010 Type II collagenase Sigma-Aldrich C2-28-100MG FGF2, salivary epithelial stem cell culture Novoprotein #C046 Wnt3a, salivary epithelial stem cell culture Novoprotein #C22R Nicotinamide Yuanye Bio #S13015 TGF-beta inhibitor A8301 KKL med #KM2595 CHIR, salivary epithelial stem cell culture InvivoChem #V2805 DMEM/F-12, mammary tumor organoid digestion Gibco #12634010 Trypsin-EDTA, 0.25% GENOM GNM25200-5 Reagent or resource Source Identifier Dispase, mammary tumor organoid digestion buffer II STEMCELL Technologies (written as Stemcell) #07923 DMEM GENOM #GNM12320 Antibiotics for HEK293/ HEK293T culture TransGen Penicillin/ Streptomycin, FG101-01 Polybrene Yeasen #40804ES76 Lipofectamine 2000 invitrogen 52887 GSK-3 inhibitor BIO Calbiochem #361552 Dispase, organoid immunofluorescence (5 U/mL) STEMCELL Technologies 07913 Paraformaldehyde (PFA), 4% fixation Biosharp BL539A Triton X-100 Solarbio T8200 BSA BioFroxx 4240GR100 DAPI Solarbio C0060 Fructose-glycerol clearing/mounting medium Prepared in-house from D-(-)-fructose (Sangon) and glycerol (Macklin) Fructose A6002130500; glycerol G810575 Ethanol Sinopharm 64-17-5 Chloroform Sinopharm 67-66-3 Glacial acetic acid Sinopharm 10000208 Carmine Sigma 237086 Aluminum potassium sulfate dodecahydrate Sigma C1022-25G Xylene Sinopharm 1330-20-7 Methyl salicylate D&B K898001 Glycerol Macklin G810575 Hematoxylin Servicebio G1004 Hydrochloric ethanol differentiator solution Servicebio G1039 Eosin solution Servicebio G1001 Neutral mounting glue Solarbio Neutral balsam, G8590 TRIzol Thermo Fisher #15596026 Protease inhibitor cocktail MCE #HY-K0010 Glycine Solarbio G8200-5kg PBS GENOM GNM20012-5 Phenol-chloroform BioFlux BSA03M1 DMEM for mammary stem cell transplantation mixture GENOM DMEM high glucose, GNM12800-5 Critical commercial assays, kits, probes, and |