prime script rt reagent kit (TaKaRa)

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TaKaRa prime script rt reagent kit
Prime Script Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 88818 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+rt+kit/PrimeScript+RT+Reagent+Kit/pm42801782-96-21-26
Average 99 stars, based on 88818 article reviews

prime script rt reagent kit - by Bioz Stars, 2026-10

99/100 stars

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Synthesized:

Article Title: Diagnostic and mechanistic roles of miR-5584-5p in endometriosis via the FZD2-mediated Wnt/β-catenin EMT axis.
Article Snippet: .. cDNA was synthesized from qualified RNA using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara, Japan) on a T100 Thermal Cycler (Bio-Rad, USA). .. qPCR was conducted on a QuantStudio 5 System (Applied Biosystems, USA) with SYBR Premix Ex Taq II (Takara, Japan) under the following conditions: 95 °C for 30 s; 38 cycles of 95 °C for 5 s and 60 °C for 30 s. Melt curve analysis confirmed amplification specificity.

Article Title: An Anoikis-Related Gene Signature Associated With Stromal Remodeling and Clinical Outcome in Gastric Cancer.
Article Snippet: .. For quantitative real- time PCR (qRT- PCR), total RNA was extracted using Total RNA Extraction Reagent (ABclonal, USA, RK30129), and complementary DNA (cDNA) was synthesized using the PrimeScript RT Reagent Kit (TaKaRa, Japan, RR037A). .. qRT- PCR was performed on a Bio- Rad CFX96 system (Bio- Rad, USA) using SYBR Premix Ex Taq II (TaKaRa, Japan, RR420A).

Article Title: USP36-mediated stabilization of NAT10 promotes osteosarcoma progression via ac4C-dependent KIF23 mRNA stabilization.
Article Snippet: Osteosarcoma is an aggressive bone malignancy with a propensity for pulmonary metastasis, yet the molecular mechanisms linking protein homeostasis to epitranscriptomic regulation remain incompletely understood.. Here, integrated transcriptomic, survival, protein-interaction, and CRISPR dependency analyses identified the deubiquitinase USP36 as a driver of osteosarcoma progression.. USP36 was elevated in osteosarcoma datasets, and higher expression was associated with poorer survival in univariable analysis.

Recombinant:

Article Title: Fungus Rhizopus arrhizus promotes MASLD-associated hepatocellular carcinoma through generating kynurenic acid.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant IQGAP1 protein Recombx Gene N/A Diethylnitrosamine Sigma-Aldrich Cat# N0756 TRIzol Reagent Thermo Fisher Scientific Cat# 15596026 3-(4,5-dimethylthiazol-2-yl)-2,5diphenyltetrazolium bromide (MTT) Invitrogen Cat# M6494 Lipofectamine 2000 Transfection Reagent Thermo Fisher Scientific Cat# 11668019 RNAiMAX Transfection Reagent Thermo Fisher Scientific Cat# 13778150 CH-223191 MedChemExpress Cat# HY-12684 ML 145 MedChemExpress Cat# HY-107536 Critical commercial assays PI/RNase Staining Buffer BD Biosciences Cat# 550825 Annexin V-FITC/PI BD Biosciences Cat# 556547 Foxp3/Transcription Factor Staining Buffer Set eBioscience Cat# 00-5521-00 PrimeScript RT reagent Kit Takara Cat# RR037A ALT Kit IDEXX 98-11067-01 AST Kit IDEXX 98-11069-01 TB Green Premix Takara Cat# RR420A FISH Auxiliary Kit JnBioscience Cat# V-001 Membrane and Cytosol Protein Extraction Kit Beyotime Cat# P0033 PowerSoil Pro Kit Qiagen Cat# ID. .. 47014 QIAamp DNA Micro Kit Qiagen Cat# ID.

MTT Assay:

Transfection:

Staining:

AST Assay:

FISH:

Membrane:

Protein Extraction:

Real-time Polymerase Chain Reaction:

Article Title: Network toxicology, molecular docking and molecular dynamics simulations for bisphenol A neurotoxicity in depression pathogenesis
Article Snippet: .. qPCR: Total RNA was extracted from brain tissues using TRIzol reagent (Invitrogen, USA) and reverse-transcribed into cDNA using a PrimeScript RT reagent kit (Takara, Japan). .. qPCR was performed using SYBR Green Master Mix (Takara) on a LightCycler 480 system (Roche).

Quantitative RT-PCR:

RNA Extraction:

Reverse Transcription:

Article Title: RPARP‐AS1 Targets miR‐10b‐5p to Influence Prognosis in Non‐Small Cell Lung Cancer and Inhibit Tumor Progression
Article Snippet: The quality of the extracted RNA was assessed using a Nanodrop 8000 microvolume nucleic acid quantifier. .. Besides, reverse transcription of RPARP‐AS1 and miR‐10b‐5p was performed using the HiFiScript cDNA Synthesis Kit (CW2569, Cwbiotech, Taizhou) and the PrimeScript RT Reagent Kit (RR037, TAKARA, Japan), respectively. .. Ultimately, RT‐qPCR reactions for lncRNA and miRNA were separately conducted with the Ultra SYBR Mixture (CW0957, Cwbiotech, Taizhou) and miRNA qPCR Assay Kit (CW2142, Cwbiotech, Taizhou), with the specific conditions set as follows: 95°C for 10 min, 95°C for 15 s, 60°C for 1 min, and repeated 42 times.

Article Title: Yeast β-1,3/1,6 glucan regulates NRF2 to inhibit ferroptosis and ameliorates aspiration pneumonia in aged mice.
Article Snippet: Background: Aspiration pneumonia (AP) is a common and serious respiratory disease among the elderly, with current treatments limited primarily to antibiotics and supportive care, highlighting a need for targeted therapies.. Yeast β-1,3/1,6glucan (YBG), a natural polysaccharide derived from yeast cell walls, holds immunomodulatory and antioxidant properties.. However, its mechanism in AP remains unclear.

cDNA Synthesis:

Concentration Assay:

Article Title: Lipoprotein biology during induced oogenesis in the short-finned eel, Anguilla australis —hepatic target gene expression and accumulation of nutrient sources into developing oocytes
Article Snippet: After RNA quantification by spectrophotometry, 5 μg of total RNA was treated with TURBO DNA-free (Ambion), to reduce genomic DNA contamination. .. The concentration of DNase-treated RNA was determined using a Quant-it RiboGreen RNA assay kit (Invitrogen), after which complementary DNA (cDNA) was synthesised by reverse transcribing 500 ng of total RNA (PrimeScript RT reagent Kit; Takara) according to the manufacturer’s protocol. ..

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