one step sybr prime script rtpcr kit (TaKaRa)
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TaKaRa
one step sybr prime script rtpcr kit
One Step Sybr Prime Script Rtpcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10987 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+one+step+rtpcr+kit/PrimeScript+RT-PCR+Kit/pm37714433-79-29-35
Average 98 stars, based on 10987 article reviews
One Step Sybr Prime Script Rtpcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10987 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+one+step+rtpcr+kit/PrimeScript+RT-PCR+Kit/pm37714433-79-29-35
Average 98 stars, based on 10987 article reviews
one step sybr prime script rtpcr kit - by Bioz Stars,
2026-11
98/100 stars
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1) Product Images from "Classical swine fever virus NS5A protein antagonizes innate immune response by inhibiting the NF-κB signaling."
Article Title: Classical swine fever virus NS5A protein antagonizes innate immune response by inhibiting the NF-κB signaling.
Journal: Virologica Sinica
doi: 10.1016/j.virs.2023.09.002
Figure Legend Snippet: Fig. 4. Determination of the region of NS5A required for inhibition of the NF-κB signaling. A–C HEK293T cells in 6-well plate were transfected with full-length NS5A or NS5A truncated mutant (2.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and then harvested for detection of IKKα/β phos- phorylation (A), IκBα degradation (B), quantification of IFN-α mRNA (C). The expressing level of phosphorylated IKKα/β and IκBα was analyzed by Western blotting. IFN-α mRNA was detected by RT-PCR using the One-Step SYBR Prime Script RT-PCR kit. D HEK293T cells in 24-well plate were transfected with pNF-κB-Luc reporter (1 μg/well), PRL-TK internal control (0.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and the NF-κB transcriptional activity was analyzed using the Dual-Lumi Luciferase Reporter Gene Assay Kit. E The expression level of NS5A and its truncated mutants in above experiments was detected by Western blotting. Data presented as means SD. Statistical analyses were performed using GraphPad Prism version 6.01 software (GraphPad Software, La Jolla, CA, USA).**, P < 0.01; ***, P < 0.001; NS, not significant.
Techniques Used: Inhibition, Transfection, Mutagenesis, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Control, Activity Assay, Luciferase, Reporter Gene Assay, Software
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