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    TaKaRa one step sybr prime script rtpcr kit
    Fig. 4. Determination of the region of NS5A required for inhibition of the NF-κB signaling. A–C HEK293T cells in 6-well plate were transfected with full-length NS5A or NS5A truncated mutant (2.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and then harvested for detection of IKKα/β phos- phorylation (A), IκBα degradation (B), quantification of IFN-α mRNA (C). The expressing level of phosphorylated IKKα/β and IκBα was analyzed by Western blotting. IFN-α mRNA was detected by RT-PCR using the One-Step <t>SYBR</t> Prime Script RT-PCR kit. D HEK293T cells in 24-well plate were transfected with pNF-κB-Luc reporter (1 μg/well), PRL-TK internal control (0.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and the NF-κB transcriptional activity was analyzed using the Dual-Lumi Luciferase Reporter Gene Assay Kit. E The expression level of NS5A and its truncated mutants in above experiments was detected by Western blotting. Data presented as means SD. Statistical analyses were performed using GraphPad Prism version 6.01 software (GraphPad Software, La Jolla, CA, USA).**, P < 0.01; ***, P < 0.001; NS, not significant.
    One Step Sybr Prime Script Rtpcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 10947 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/prime+script+one+step+rtpcr+kit/PrimeScript+RT-PCR+Kit/pm37714433-79-29-35
    Average 98 stars, based on 10947 article reviews
    one step sybr prime script rtpcr kit - by Bioz Stars, 2026-10
    98/100 stars

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    1) Product Images from "Classical swine fever virus NS5A protein antagonizes innate immune response by inhibiting the NF-κB signaling."

    Article Title: Classical swine fever virus NS5A protein antagonizes innate immune response by inhibiting the NF-κB signaling.

    Journal: Virologica Sinica

    doi: 10.1016/j.virs.2023.09.002

    Fig. 4. Determination of the region of NS5A required for inhibition of the NF-κB signaling. A–C HEK293T cells in 6-well plate were transfected with full-length NS5A or NS5A truncated mutant (2.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and then harvested for detection of IKKα/β phos- phorylation (A), IκBα degradation (B), quantification of IFN-α mRNA (C). The expressing level of phosphorylated IKKα/β and IκBα was analyzed by Western blotting. IFN-α mRNA was detected by RT-PCR using the One-Step SYBR Prime Script RT-PCR kit. D HEK293T cells in 24-well plate were transfected with pNF-κB-Luc reporter (1 μg/well), PRL-TK internal control (0.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and the NF-κB transcriptional activity was analyzed using the Dual-Lumi Luciferase Reporter Gene Assay Kit. E The expression level of NS5A and its truncated mutants in above experiments was detected by Western blotting. Data presented as means SD. Statistical analyses were performed using GraphPad Prism version 6.01 software (GraphPad Software, La Jolla, CA, USA).**, P < 0.01; ***, P < 0.001; NS, not significant.
    Figure Legend Snippet: Fig. 4. Determination of the region of NS5A required for inhibition of the NF-κB signaling. A–C HEK293T cells in 6-well plate were transfected with full-length NS5A or NS5A truncated mutant (2.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and then harvested for detection of IKKα/β phos- phorylation (A), IκBα degradation (B), quantification of IFN-α mRNA (C). The expressing level of phosphorylated IKKα/β and IκBα was analyzed by Western blotting. IFN-α mRNA was detected by RT-PCR using the One-Step SYBR Prime Script RT-PCR kit. D HEK293T cells in 24-well plate were transfected with pNF-κB-Luc reporter (1 μg/well), PRL-TK internal control (0.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and the NF-κB transcriptional activity was analyzed using the Dual-Lumi Luciferase Reporter Gene Assay Kit. E The expression level of NS5A and its truncated mutants in above experiments was detected by Western blotting. Data presented as means SD. Statistical analyses were performed using GraphPad Prism version 6.01 software (GraphPad Software, La Jolla, CA, USA).**, P < 0.01; ***, P < 0.001; NS, not significant.

    Techniques Used: Inhibition, Transfection, Mutagenesis, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Control, Activity Assay, Luciferase, Reporter Gene Assay, Software

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    Article Title: Establishment and preliminary evaluation of an RT-ERA combined with CRISPR/Cas12a assay for detection of H5 and H7 influenza viruses
    Article Snippet: .. RT-qPCR was performed using the 2×One Step PrimeScript RT-PCR Kit (Takara) with the following conditions: reverse transcription at 42 °C for 5 min, initial denaturation at 95 °C for 10 s, followed by 40 cycles of 95 °C for 5 s and 60 °C for 34 s. Fluorescence signals were acquired at the 60 °C annealing-extension step. ..

    Real-time Polymerase Chain Reaction:

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    Fig. 4. Determination of the region of NS5A required for inhibition of the NF-κB signaling. A–C HEK293T cells in 6-well plate were transfected with full-length NS5A or NS5A truncated mutant (2.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and then harvested for detection of IKKα/β phos- phorylation (A), IκBα degradation (B), quantification of IFN-α mRNA (C). The expressing level of phosphorylated IKKα/β and IκBα was analyzed by Western blotting. IFN-α mRNA was detected by RT-PCR using the One-Step <t>SYBR</t> Prime Script RT-PCR kit. D HEK293T cells in 24-well plate were transfected with pNF-κB-Luc reporter (1 μg/well), PRL-TK internal control (0.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and the NF-κB transcriptional activity was analyzed using the Dual-Lumi Luciferase Reporter Gene Assay Kit. E The expression level of NS5A and its truncated mutants in above experiments was detected by Western blotting. Data presented as means SD. Statistical analyses were performed using GraphPad Prism version 6.01 software (GraphPad Software, La Jolla, CA, USA).**, P < 0.01; ***, P < 0.001; NS, not significant.
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    Fig. 4. Determination of the region of NS5A required for inhibition of the NF-κB signaling. A–C HEK293T cells in 6-well plate were transfected with full-length NS5A or NS5A truncated mutant (2.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and then harvested for detection of IKKα/β phos- phorylation (A), IκBα degradation (B), quantification of IFN-α mRNA (C). The expressing level of phosphorylated IKKα/β and IκBα was analyzed by Western blotting. IFN-α mRNA was detected by RT-PCR using the One-Step SYBR Prime Script RT-PCR kit. D HEK293T cells in 24-well plate were transfected with pNF-κB-Luc reporter (1 μg/well), PRL-TK internal control (0.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and the NF-κB transcriptional activity was analyzed using the Dual-Lumi Luciferase Reporter Gene Assay Kit. E The expression level of NS5A and its truncated mutants in above experiments was detected by Western blotting. Data presented as means SD. Statistical analyses were performed using GraphPad Prism version 6.01 software (GraphPad Software, La Jolla, CA, USA).**, P < 0.01; ***, P < 0.001; NS, not significant.

    Journal: Virologica Sinica

    Article Title: Classical swine fever virus NS5A protein antagonizes innate immune response by inhibiting the NF-κB signaling.

    doi: 10.1016/j.virs.2023.09.002

    Figure Lengend Snippet: Fig. 4. Determination of the region of NS5A required for inhibition of the NF-κB signaling. A–C HEK293T cells in 6-well plate were transfected with full-length NS5A or NS5A truncated mutant (2.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and then harvested for detection of IKKα/β phos- phorylation (A), IκBα degradation (B), quantification of IFN-α mRNA (C). The expressing level of phosphorylated IKKα/β and IκBα was analyzed by Western blotting. IFN-α mRNA was detected by RT-PCR using the One-Step SYBR Prime Script RT-PCR kit. D HEK293T cells in 24-well plate were transfected with pNF-κB-Luc reporter (1 μg/well), PRL-TK internal control (0.5 μg/well). After 48 h, the cells were treated with or without TNF-α for 15 min and the NF-κB transcriptional activity was analyzed using the Dual-Lumi Luciferase Reporter Gene Assay Kit. E The expression level of NS5A and its truncated mutants in above experiments was detected by Western blotting. Data presented as means SD. Statistical analyses were performed using GraphPad Prism version 6.01 software (GraphPad Software, La Jolla, CA, USA).**, P < 0.01; ***, P < 0.001; NS, not significant.

    Article Snippet: At 48 h post-transfection, the cells were stimulated with or without TNF-α (final concentration of 20 ng/mL) for 15 min and then harvested for quantification of IFN-αmRNA using the One-Step SYBR Prime Script RTPCR kit (TaKaRa, Dalian, China) according to the manufacturer's protocol.

    Techniques: Inhibition, Transfection, Mutagenesis, Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Control, Activity Assay, Luciferase, Reporter Gene Assay, Software