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pichia expression vector ppiczαa  (Thermo Fisher)


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    Thermo Fisher pichia expression vector ppiczαa
    Pichia Expression Vector Ppiczαa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ppicz%CE%B1a+expression+vector/pmc03355165-167-22-26
    Average 90 stars, based on 1 article reviews
    pichia expression vector ppiczαa - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Crystallization and preliminary X-ray analysis of Na-SAA-2 from the human hookworm parasite Necator americanus
    Article Snippet: .. The PCR product was subcloned into the Pichia expression vector pPICZαA (Invitrogen) through Xho I and Xba I sites. ..

    Article Title: Porcine CD 38 exhibits prominent secondary NAD + cyclase activity
    Article Snippet: .. For the expression of recombinant pCD38 ectodomain in Pichia pastoris , DNA fragment harboring the pCD38 ectodomain was amplified by PCR and cloned into the Pichia expression vector pPICZαA (Invitrogen) in‐frame with the yeast mating α‐factor signal sequence. .. The predicted glycosylation site of pCD38 ectodomain, Asn213, was mutated to an Asp using Quikchange site‐directed mutagenesis kit (Stratagene) according to the manufacturer's protocol.

    Article Title: X-ray structures of Na -GST-1 and Na -GST-2 two glutathione s-transferase from the human hookworm Necator americanus
    Article Snippet: .. The PCR products were sub-cloned into the Pichia expression vector pPICZαA (Invitrogen) via the XhoI and XbaI sites. ..

    Expressing:

    Article Title: Crystallization and preliminary X-ray analysis of Na-SAA-2 from the human hookworm parasite Necator americanus
    Article Snippet: .. The PCR product was subcloned into the Pichia expression vector pPICZαA (Invitrogen) through Xho I and Xba I sites. ..

    Article Title: Porcine CD 38 exhibits prominent secondary NAD + cyclase activity
    Article Snippet: .. For the expression of recombinant pCD38 ectodomain in Pichia pastoris , DNA fragment harboring the pCD38 ectodomain was amplified by PCR and cloned into the Pichia expression vector pPICZαA (Invitrogen) in‐frame with the yeast mating α‐factor signal sequence. .. The predicted glycosylation site of pCD38 ectodomain, Asn213, was mutated to an Asp using Quikchange site‐directed mutagenesis kit (Stratagene) according to the manufacturer's protocol.

    Article Title: X-ray structures of Na -GST-1 and Na -GST-2 two glutathione s-transferase from the human hookworm Necator americanus
    Article Snippet: .. The PCR products were sub-cloned into the Pichia expression vector pPICZαA (Invitrogen) via the XhoI and XbaI sites. ..

    Article Title: Characterisation of three fungal glucuronoyl esterases on glucuronic acid ester model compounds
    Article Snippet: .. Codon-optimised genes excluding signal peptide were synthesised (NZYTech, Portugal) and cloned into the Pichia expression vector pPICZαA (Thermo Fisher Scientific) using FastDigest Eco RI and FastDigest Xba I (Thermo Fisher Scientific) in frame with the N-terminal α-factor and the C-terminal myc- and 6XHis-tag. .. Briefly, DNA molecules were purified using the Illustra GFX GelBand purification kit (GE Healthcare) and vector and respective gene inserts ligated with T4 DNA ligase according to the manufacturer’s protocol (Thermo Fisher Scientific).

    Article Title: The Adjuvanticity of an O. volvulus -Derived r Ov -ASP-1 Protein in Mice Using Sequential Vaccinations and in Non-Human Primates
    Article Snippet: .. Yeast codon optimized Ov - asp -1 cDNA sequence (AF020586) without the region encoding the N-terminal signal peptide was cloned into the Pichia expression vector pPICZαA (Invitrogen) using the Eco RI and Xba I restriction sites. ..

    Plasmid Preparation:

    Article Title: Crystallization and preliminary X-ray analysis of Na-SAA-2 from the human hookworm parasite Necator americanus
    Article Snippet: .. The PCR product was subcloned into the Pichia expression vector pPICZαA (Invitrogen) through Xho I and Xba I sites. ..

    Article Title: Porcine CD 38 exhibits prominent secondary NAD + cyclase activity
    Article Snippet: .. For the expression of recombinant pCD38 ectodomain in Pichia pastoris , DNA fragment harboring the pCD38 ectodomain was amplified by PCR and cloned into the Pichia expression vector pPICZαA (Invitrogen) in‐frame with the yeast mating α‐factor signal sequence. .. The predicted glycosylation site of pCD38 ectodomain, Asn213, was mutated to an Asp using Quikchange site‐directed mutagenesis kit (Stratagene) according to the manufacturer's protocol.

    Article Title: X-ray structures of Na -GST-1 and Na -GST-2 two glutathione s-transferase from the human hookworm Necator americanus
    Article Snippet: .. The PCR products were sub-cloned into the Pichia expression vector pPICZαA (Invitrogen) via the XhoI and XbaI sites. ..

    Article Title: Characterisation of three fungal glucuronoyl esterases on glucuronic acid ester model compounds
    Article Snippet: .. Codon-optimised genes excluding signal peptide were synthesised (NZYTech, Portugal) and cloned into the Pichia expression vector pPICZαA (Thermo Fisher Scientific) using FastDigest Eco RI and FastDigest Xba I (Thermo Fisher Scientific) in frame with the N-terminal α-factor and the C-terminal myc- and 6XHis-tag. .. Briefly, DNA molecules were purified using the Illustra GFX GelBand purification kit (GE Healthcare) and vector and respective gene inserts ligated with T4 DNA ligase according to the manufacturer’s protocol (Thermo Fisher Scientific).

    Article Title: Hetero‐trans‐β‐glucanase, an enzyme unique to Equisetum plants, functionalizes cellulose
    Article Snippet: .. To express EfHTG as a fusion protein from the Pichia pPICZαA vector (Life Technologies), we amplified cDNA with Phusion (New England Biolabs; www.neb.com ) polymerase and HTG‐specific primers, which introduced a 5′ Eco RI site immediately upstream of the sequence encoding the putative N‐terminus of mature EfHTG, and replaced the EfHTG stop codon with an Xba I site. ..

    Article Title: The Adjuvanticity of an O. volvulus -Derived r Ov -ASP-1 Protein in Mice Using Sequential Vaccinations and in Non-Human Primates
    Article Snippet: .. Yeast codon optimized Ov - asp -1 cDNA sequence (AF020586) without the region encoding the N-terminal signal peptide was cloned into the Pichia expression vector pPICZαA (Invitrogen) using the Eco RI and Xba I restriction sites. ..

    Recombinant:

    Article Title: Porcine CD 38 exhibits prominent secondary NAD + cyclase activity
    Article Snippet: .. For the expression of recombinant pCD38 ectodomain in Pichia pastoris , DNA fragment harboring the pCD38 ectodomain was amplified by PCR and cloned into the Pichia expression vector pPICZαA (Invitrogen) in‐frame with the yeast mating α‐factor signal sequence. .. The predicted glycosylation site of pCD38 ectodomain, Asn213, was mutated to an Asp using Quikchange site‐directed mutagenesis kit (Stratagene) according to the manufacturer's protocol.

    Amplification:

    Article Title: Porcine CD 38 exhibits prominent secondary NAD + cyclase activity
    Article Snippet: .. For the expression of recombinant pCD38 ectodomain in Pichia pastoris , DNA fragment harboring the pCD38 ectodomain was amplified by PCR and cloned into the Pichia expression vector pPICZαA (Invitrogen) in‐frame with the yeast mating α‐factor signal sequence. .. The predicted glycosylation site of pCD38 ectodomain, Asn213, was mutated to an Asp using Quikchange site‐directed mutagenesis kit (Stratagene) according to the manufacturer's protocol.

    Article Title: Hetero‐trans‐β‐glucanase, an enzyme unique to Equisetum plants, functionalizes cellulose
    Article Snippet: .. To express EfHTG as a fusion protein from the Pichia pPICZαA vector (Life Technologies), we amplified cDNA with Phusion (New England Biolabs; www.neb.com ) polymerase and HTG‐specific primers, which introduced a 5′ Eco RI site immediately upstream of the sequence encoding the putative N‐terminus of mature EfHTG, and replaced the EfHTG stop codon with an Xba I site. ..

    Clone Assay:

    Article Title: Porcine CD 38 exhibits prominent secondary NAD + cyclase activity
    Article Snippet: .. For the expression of recombinant pCD38 ectodomain in Pichia pastoris , DNA fragment harboring the pCD38 ectodomain was amplified by PCR and cloned into the Pichia expression vector pPICZαA (Invitrogen) in‐frame with the yeast mating α‐factor signal sequence. .. The predicted glycosylation site of pCD38 ectodomain, Asn213, was mutated to an Asp using Quikchange site‐directed mutagenesis kit (Stratagene) according to the manufacturer's protocol.

    Article Title: Characterisation of three fungal glucuronoyl esterases on glucuronic acid ester model compounds
    Article Snippet: .. Codon-optimised genes excluding signal peptide were synthesised (NZYTech, Portugal) and cloned into the Pichia expression vector pPICZαA (Thermo Fisher Scientific) using FastDigest Eco RI and FastDigest Xba I (Thermo Fisher Scientific) in frame with the N-terminal α-factor and the C-terminal myc- and 6XHis-tag. .. Briefly, DNA molecules were purified using the Illustra GFX GelBand purification kit (GE Healthcare) and vector and respective gene inserts ligated with T4 DNA ligase according to the manufacturer’s protocol (Thermo Fisher Scientific).

    Article Title: The Adjuvanticity of an O. volvulus -Derived r Ov -ASP-1 Protein in Mice Using Sequential Vaccinations and in Non-Human Primates
    Article Snippet: .. Yeast codon optimized Ov - asp -1 cDNA sequence (AF020586) without the region encoding the N-terminal signal peptide was cloned into the Pichia expression vector pPICZαA (Invitrogen) using the Eco RI and Xba I restriction sites. ..

    Sequencing:

    Article Title: Porcine CD 38 exhibits prominent secondary NAD + cyclase activity
    Article Snippet: .. For the expression of recombinant pCD38 ectodomain in Pichia pastoris , DNA fragment harboring the pCD38 ectodomain was amplified by PCR and cloned into the Pichia expression vector pPICZαA (Invitrogen) in‐frame with the yeast mating α‐factor signal sequence. .. The predicted glycosylation site of pCD38 ectodomain, Asn213, was mutated to an Asp using Quikchange site‐directed mutagenesis kit (Stratagene) according to the manufacturer's protocol.

    Article Title: Hetero‐trans‐β‐glucanase, an enzyme unique to Equisetum plants, functionalizes cellulose
    Article Snippet: .. To express EfHTG as a fusion protein from the Pichia pPICZαA vector (Life Technologies), we amplified cDNA with Phusion (New England Biolabs; www.neb.com ) polymerase and HTG‐specific primers, which introduced a 5′ Eco RI site immediately upstream of the sequence encoding the putative N‐terminus of mature EfHTG, and replaced the EfHTG stop codon with an Xba I site. ..

    Article Title: The Adjuvanticity of an O. volvulus -Derived r Ov -ASP-1 Protein in Mice Using Sequential Vaccinations and in Non-Human Primates
    Article Snippet: .. Yeast codon optimized Ov - asp -1 cDNA sequence (AF020586) without the region encoding the N-terminal signal peptide was cloned into the Pichia expression vector pPICZαA (Invitrogen) using the Eco RI and Xba I restriction sites. ..

    Yeast:

    Article Title: The Adjuvanticity of an O. volvulus -Derived r Ov -ASP-1 Protein in Mice Using Sequential Vaccinations and in Non-Human Primates
    Article Snippet: .. Yeast codon optimized Ov - asp -1 cDNA sequence (AF020586) without the region encoding the N-terminal signal peptide was cloned into the Pichia expression vector pPICZαA (Invitrogen) using the Eco RI and Xba I restriction sites. ..



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