yeast expression vector ppiczαa (Thermo Fisher)
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yeast expression vector ppiczαa
Yeast Expression Vector Ppiczαa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppicz%CE%B1a+expression+vector/pmc03252255-159-14-19
Average 90 stars, based on 1 article reviews
Yeast Expression Vector Ppiczαa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppicz%CE%B1a+expression+vector/pmc03252255-159-14-19
Average 90 stars, based on 1 article reviews
yeast expression vector ppiczαa - by Bioz Stars,
2026-10
90/100 stars
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Expressing:Article Title: The recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium , Colletotrichum and Phakopsora Article Snippet: .. The drr230a gene was subcloned into the Article Title: Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production Article Snippet: .. Restriction enzymes and T4-ligase were purchased from Fermentas, Phusion polymerase from New England Biolabs and the Article Title: Vaccination of Gerbils with Bm -103 and Bm -RAL-2 Concurrently or as a Fusion Protein Confers Consistent and Improved Protection against Brugia malayi Infection Article Snippet: .. The DNA coding for Bm -103 without the C-terminal transmembrane domain was codon optimized and then synthesized by GenScript (Piscataway, NJ, USA) and subcloned in-frame into the Article Title: Trichuris muris whey acidic protein induces type 2 protective immunity against whipworm Article Snippet: .. DNA encoding for the Tm -WAP49, comprising 70% of the immunodominant Tm -WAP (amino acids 24–393), was amplified without signal peptide from the total T . muris adult cDNA using forward (5’-GCGAATTCGCTAAAATAGGTTCATGTCC-3’) and reverse (5’-ATGCGGCCGCTCAATGATGATGATGATGATGCTCGGCAGTGCTGTCGTCTCGGT-3’) primers and subcloned into Article Title: Expression and purification of an engineered, yeast-expressed Leishmania donovani nucleoside hydrolase with immunogenic properties Article Snippet: The genetically engineered DNA sequence for all 3 constructs was codon optimized based on yeast codon preference, synthesized by GeneScript (Piscataway, NJ) and cloned into pUC57. .. PCR amplified coding DNA was gel purified and ligated into the Article Title: Vaccines to combat river blindness: expression, selection and formulation of vaccines against infection with Onchocerca volvulus in a mouse model Article Snippet: For expression in E. coli , the recombinant constructs cloned into pET41a were transformed into BL21(DE3) (EMDMillipore) and recombinant proteins were induced with 1 mM isopropyl-β-thiogalactoside (IPTG) and purified with IMAC as previously described ( Zhan et al., 2002 ). .. The yeast codon optimized fusion DNAs were subcloned into either Article Title: Functional Properties of Novel Epigallocatechin Gallate Glucosides Synthesized by Using Dextransucrase from Leuconostoc mesenteroides B-1299CB4 Article Snippet: Epigallocatechin gallate (EGCG) is the most abundant catechin found in the leaves of green tea, Camellia sinensis.. In this study, novel epigallocatechin gallate-glucocides (EGCG-Gs) were synthesized by using dextransucrase from Leuconostoc mesenteroides B-1299CB4.. Response surface methodology was adopted to optimize the conversion of EGCG to EGCG-Gs, resulting in a 91.43% conversion rate of EGCG. Control:Article Title: The recombinant pea defensin Drr230a is active against impacting soybean and cotton pathogenic fungi from the genera Fusarium , Colletotrichum and Phakopsora Article Snippet: .. The drr230a gene was subcloned into the Yeast:Article Title: Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production Article Snippet: .. Restriction enzymes and T4-ligase were purchased from Fermentas, Phusion polymerase from New England Biolabs and the Article Title: Functional Properties of Novel Epigallocatechin Gallate Glucosides Synthesized by Using Dextransucrase from Leuconostoc mesenteroides B-1299CB4 Article Snippet: Epigallocatechin gallate (EGCG) is the most abundant catechin found in the leaves of green tea, Camellia sinensis.. In this study, novel epigallocatechin gallate-glucocides (EGCG-Gs) were synthesized by using dextransucrase from Leuconostoc mesenteroides B-1299CB4.. Response surface methodology was adopted to optimize the conversion of EGCG to EGCG-Gs, resulting in a 91.43% conversion rate of EGCG. Plasmid Preparation:Article Title: Semi-rational engineering of cellobiose dehydrogenase for improved hydrogen peroxide production Article Snippet: .. Restriction enzymes and T4-ligase were purchased from Fermentas, Phusion polymerase from New England Biolabs and the Article Title: Vaccination of Gerbils with Bm -103 and Bm -RAL-2 Concurrently or as a Fusion Protein Confers Consistent and Improved Protection against Brugia malayi Infection Article Snippet: .. The DNA coding for Bm -103 without the C-terminal transmembrane domain was codon optimized and then synthesized by GenScript (Piscataway, NJ, USA) and subcloned in-frame into the Article Title: Expression and purification of an engineered, yeast-expressed Leishmania donovani nucleoside hydrolase with immunogenic properties Article Snippet: The genetically engineered DNA sequence for all 3 constructs was codon optimized based on yeast codon preference, synthesized by GeneScript (Piscataway, NJ) and cloned into pUC57. .. PCR amplified coding DNA was gel purified and ligated into the Article Title: Vaccines to combat river blindness: expression, selection and formulation of vaccines against infection with Onchocerca volvulus in a mouse model Article Snippet: For expression in E. coli , the recombinant constructs cloned into pET41a were transformed into BL21(DE3) (EMDMillipore) and recombinant proteins were induced with 1 mM isopropyl-β-thiogalactoside (IPTG) and purified with IMAC as previously described ( Zhan et al., 2002 ). .. The yeast codon optimized fusion DNAs were subcloned into either Article Title: Functional Properties of Novel Epigallocatechin Gallate Glucosides Synthesized by Using Dextransucrase from Leuconostoc mesenteroides B-1299CB4 Article Snippet: Epigallocatechin gallate (EGCG) is the most abundant catechin found in the leaves of green tea, Camellia sinensis.. In this study, novel epigallocatechin gallate-glucocides (EGCG-Gs) were synthesized by using dextransucrase from Leuconostoc mesenteroides B-1299CB4.. Response surface methodology was adopted to optimize the conversion of EGCG to EGCG-Gs, resulting in a 91.43% conversion rate of EGCG. Synthesized:Article Title: Vaccination of Gerbils with Bm -103 and Bm -RAL-2 Concurrently or as a Fusion Protein Confers Consistent and Improved Protection against Brugia malayi Infection Article Snippet: .. The DNA coding for Bm -103 without the C-terminal transmembrane domain was codon optimized and then synthesized by GenScript (Piscataway, NJ, USA) and subcloned in-frame into the Amplification:Article Title: Trichuris muris whey acidic protein induces type 2 protective immunity against whipworm Article Snippet: .. DNA encoding for the Tm -WAP49, comprising 70% of the immunodominant Tm -WAP (amino acids 24–393), was amplified without signal peptide from the total T . muris adult cDNA using forward (5’-GCGAATTCGCTAAAATAGGTTCATGTCC-3’) and reverse (5’-ATGCGGCCGCTCAATGATGATGATGATGATGCTCGGCAGTGCTGTCGTCTCGGT-3’) primers and subcloned into Article Title: Expression and purification of an engineered, yeast-expressed Leishmania donovani nucleoside hydrolase with immunogenic properties Article Snippet: The genetically engineered DNA sequence for all 3 constructs was codon optimized based on yeast codon preference, synthesized by GeneScript (Piscataway, NJ) and cloned into pUC57. .. PCR amplified coding DNA was gel purified and ligated into the other:Article Title: Heterologous overexpression of Glomerella cingulata FAD-dependent glucose dehydrogenase in Escherichia coli and Pichia pastoris Article Snippet: The plasmid pET-21a(+)from Novagen was used for expression in E. coli . Polymerase Chain Reaction:Article Title: Expression and purification of an engineered, yeast-expressed Leishmania donovani nucleoside hydrolase with immunogenic properties Article Snippet: The genetically engineered DNA sequence for all 3 constructs was codon optimized based on yeast codon preference, synthesized by GeneScript (Piscataway, NJ) and cloned into pUC57. .. PCR amplified coding DNA was gel purified and ligated into the Purification:Article Title: Expression and purification of an engineered, yeast-expressed Leishmania donovani nucleoside hydrolase with immunogenic properties Article Snippet: The genetically engineered DNA sequence for all 3 constructs was codon optimized based on yeast codon preference, synthesized by GeneScript (Piscataway, NJ) and cloned into pUC57. .. PCR amplified coding DNA was gel purified and ligated into the Knock-Out:Article Title: Vaccines to combat river blindness: expression, selection and formulation of vaccines against infection with Onchocerca volvulus in a mouse model Article Snippet: For expression in E. coli , the recombinant constructs cloned into pET41a were transformed into BL21(DE3) (EMDMillipore) and recombinant proteins were induced with 1 mM isopropyl-β-thiogalactoside (IPTG) and purified with IMAC as previously described ( Zhan et al., 2002 ). .. The yeast codon optimized fusion DNAs were subcloned into either Clone Assay:Article Title: Functional Properties of Novel Epigallocatechin Gallate Glucosides Synthesized by Using Dextransucrase from Leuconostoc mesenteroides B-1299CB4 Article Snippet: Epigallocatechin gallate (EGCG) is the most abundant catechin found in the leaves of green tea, Camellia sinensis.. In this study, novel epigallocatechin gallate-glucocides (EGCG-Gs) were synthesized by using dextransucrase from Leuconostoc mesenteroides B-1299CB4.. Response surface methodology was adopted to optimize the conversion of EGCG to EGCG-Gs, resulting in a 91.43% conversion rate of EGCG. |