ppcrscript-cam (sk+) plasmid (Agilent technologies)
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Ppcrscript Cam (Sk+) Plasmid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppcrscript+cam/pm12461028-15-101-104
Average 90 stars, based on 1 article reviews
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Clone Assay:Article Title: Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum beta-lactamase CTX-M-15 and of its structurally related beta-lactamase CTX-M-3. Article Snippet: Bacterial strains, cloning experiments and sequencing CTX-M-15-producing Escherichia coli 2 was from India.5 Citrobacter freundii isolate 2526/96, which was identified in Poland in 1996, was used as a blaCTX-M-3-containing strain.4 E. coli reference strain DH10B was used for cloning and expression experiments.6 Cloning was carried out with PCR products generated with primers PROM+ (5′-TGCTCTGTGGATAACTTGC-3′) and preCTX-M-3B (5′-CCGTTTCCGCTATTACAAAC-3′) annealing to the 3′-end of insertion sequence ISEcp1 located upstream of blaCTX-M-15 and downstream of blaCTX-M-15/-3, respectively (accession no. AY044436).3,4 Whole-cell DNA from E. coli 2 and C. freundii 2526/96 was used as template.5 PCR amplimers were cloned into the SrfI site of the Sequencing:Article Title: Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum beta-lactamase CTX-M-15 and of its structurally related beta-lactamase CTX-M-3. Article Snippet: Bacterial strains, cloning experiments and sequencing CTX-M-15-producing Escherichia coli 2 was from India.5 Citrobacter freundii isolate 2526/96, which was identified in Poland in 1996, was used as a blaCTX-M-3-containing strain.4 E. coli reference strain DH10B was used for cloning and expression experiments.6 Cloning was carried out with PCR products generated with primers PROM+ (5′-TGCTCTGTGGATAACTTGC-3′) and preCTX-M-3B (5′-CCGTTTCCGCTATTACAAAC-3′) annealing to the 3′-end of insertion sequence ISEcp1 located upstream of blaCTX-M-15 and downstream of blaCTX-M-15/-3, respectively (accession no. AY044436).3,4 Whole-cell DNA from E. coli 2 and C. freundii 2526/96 was used as template.5 PCR amplimers were cloned into the SrfI site of the Expressing:Article Title: Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum beta-lactamase CTX-M-15 and of its structurally related beta-lactamase CTX-M-3. Article Snippet: Bacterial strains, cloning experiments and sequencing CTX-M-15-producing Escherichia coli 2 was from India.5 Citrobacter freundii isolate 2526/96, which was identified in Poland in 1996, was used as a blaCTX-M-3-containing strain.4 E. coli reference strain DH10B was used for cloning and expression experiments.6 Cloning was carried out with PCR products generated with primers PROM+ (5′-TGCTCTGTGGATAACTTGC-3′) and preCTX-M-3B (5′-CCGTTTCCGCTATTACAAAC-3′) annealing to the 3′-end of insertion sequence ISEcp1 located upstream of blaCTX-M-15 and downstream of blaCTX-M-15/-3, respectively (accession no. AY044436).3,4 Whole-cell DNA from E. coli 2 and C. freundii 2526/96 was used as template.5 PCR amplimers were cloned into the SrfI site of the Polymerase Chain Reaction:Article Title: Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum beta-lactamase CTX-M-15 and of its structurally related beta-lactamase CTX-M-3. Article Snippet: Bacterial strains, cloning experiments and sequencing CTX-M-15-producing Escherichia coli 2 was from India.5 Citrobacter freundii isolate 2526/96, which was identified in Poland in 1996, was used as a blaCTX-M-3-containing strain.4 E. coli reference strain DH10B was used for cloning and expression experiments.6 Cloning was carried out with PCR products generated with primers PROM+ (5′-TGCTCTGTGGATAACTTGC-3′) and preCTX-M-3B (5′-CCGTTTCCGCTATTACAAAC-3′) annealing to the 3′-end of insertion sequence ISEcp1 located upstream of blaCTX-M-15 and downstream of blaCTX-M-15/-3, respectively (accession no. AY044436).3,4 Whole-cell DNA from E. coli 2 and C. freundii 2526/96 was used as template.5 PCR amplimers were cloned into the SrfI site of the Generated:Article Title: Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum beta-lactamase CTX-M-15 and of its structurally related beta-lactamase CTX-M-3. Article Snippet: Bacterial strains, cloning experiments and sequencing CTX-M-15-producing Escherichia coli 2 was from India.5 Citrobacter freundii isolate 2526/96, which was identified in Poland in 1996, was used as a blaCTX-M-3-containing strain.4 E. coli reference strain DH10B was used for cloning and expression experiments.6 Cloning was carried out with PCR products generated with primers PROM+ (5′-TGCTCTGTGGATAACTTGC-3′) and preCTX-M-3B (5′-CCGTTTCCGCTATTACAAAC-3′) annealing to the 3′-end of insertion sequence ISEcp1 located upstream of blaCTX-M-15 and downstream of blaCTX-M-15/-3, respectively (accession no. AY044436).3,4 Whole-cell DNA from E. coli 2 and C. freundii 2526/96 was used as template.5 PCR amplimers were cloned into the SrfI site of the Plasmid Preparation:Article Title: Biochemical analysis of the ceftazidime-hydrolysing extended-spectrum beta-lactamase CTX-M-15 and of its structurally related beta-lactamase CTX-M-3. Article Snippet: Bacterial strains, cloning experiments and sequencing CTX-M-15-producing Escherichia coli 2 was from India.5 Citrobacter freundii isolate 2526/96, which was identified in Poland in 1996, was used as a blaCTX-M-3-containing strain.4 E. coli reference strain DH10B was used for cloning and expression experiments.6 Cloning was carried out with PCR products generated with primers PROM+ (5′-TGCTCTGTGGATAACTTGC-3′) and preCTX-M-3B (5′-CCGTTTCCGCTATTACAAAC-3′) annealing to the 3′-end of insertion sequence ISEcp1 located upstream of blaCTX-M-15 and downstream of blaCTX-M-15/-3, respectively (accession no. AY044436).3,4 Whole-cell DNA from E. coli 2 and C. freundii 2526/96 was used as template.5 PCR amplimers were cloned into the SrfI site of the |


