ppcrscript (+) vector (Agilent technologies)
Structured Review
Ppcrscript (+) Vector, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ppcrscript+(+)+vector/pm15653807-138-18-21
Average 90 stars, based on 1 article reviews
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Polymerase Chain Reaction:Article Title: The Fn14 cytoplasmic tail binds tumour-necrosis-factor-receptor-associated factors 1, 2, 3 and 5 and mediates nuclear factor-kappaB activation Article Snippet: For all membranes, bound secondary antibodies were detected using the enhanced chemiluminescence (ECL2) system (Amersham Life Science, Piscataway, NJ, U.S.A.). .. Construction of the Fn14 and TRAF plasmids for yeast two-hybrid analysis The PCR overlap extension method [21] was used to clone mutated forms of the murine Fn14 cytoplasmic tail nucleotide sequence (encoding aa residues 102–129) into the Article Title: Differential expression of 17beta-hydroxysteroid dehydrogenases types 2 and 4 in human endometrial epithelial cell lines. Article Snippet: In the endometrium two enzymes are known to convert estradiol to its inactive metabolite estrone: microsomal 17 -hydroxysteroid dehydrogenase type 2 (17 -HSD2) and peroxisomal 17 -HSD4.. In order to elucidate the particular function of each of these two different enzymes, the human endometrial epithelial cell lines HEC-1-A and RL95–2 were examined with respect to the expression of 17 HSD isozymes.. They were compared with human endometrium in vivo. Article Title: Cloning, expression and characterisation of FKB-6, the sole large TPR-containing immunophilin from C. elegans. Article Snippet: We have cloned, expressed, purified and characterised ceFKB-6, the only large tetratricopeptide repeat motif-containing immunophilin in Caenorhabditis elegans which is similar to the human orthologues FKBP51 and FKBP52.. It shows increased peptidyl prolyl isomerase activity, the measured kcat/Km of 1.3 · 10 M 1 s is twofold greater than that of hFKBP12 and hFKBP51.. NMR studies of the interaction between FKB-6 and the C-terminal DAF-21 pentapeptide MEEVD show interactions consistent with those found between the large human immunophilin TPR domains and human Hsp90. Sequencing:Article Title: The Fn14 cytoplasmic tail binds tumour-necrosis-factor-receptor-associated factors 1, 2, 3 and 5 and mediates nuclear factor-kappaB activation Article Snippet: For all membranes, bound secondary antibodies were detected using the enhanced chemiluminescence (ECL2) system (Amersham Life Science, Piscataway, NJ, U.S.A.). .. Construction of the Fn14 and TRAF plasmids for yeast two-hybrid analysis The PCR overlap extension method [21] was used to clone mutated forms of the murine Fn14 cytoplasmic tail nucleotide sequence (encoding aa residues 102–129) into the Clone Assay:Article Title: Differential expression of 17beta-hydroxysteroid dehydrogenases types 2 and 4 in human endometrial epithelial cell lines. Article Snippet: In the endometrium two enzymes are known to convert estradiol to its inactive metabolite estrone: microsomal 17 -hydroxysteroid dehydrogenase type 2 (17 -HSD2) and peroxisomal 17 -HSD4.. In order to elucidate the particular function of each of these two different enzymes, the human endometrial epithelial cell lines HEC-1-A and RL95–2 were examined with respect to the expression of 17 HSD isozymes.. They were compared with human endometrium in vivo. In Vivo:Article Title: Differential expression of 17beta-hydroxysteroid dehydrogenases types 2 and 4 in human endometrial epithelial cell lines. Article Snippet: In the endometrium two enzymes are known to convert estradiol to its inactive metabolite estrone: microsomal 17 -hydroxysteroid dehydrogenase type 2 (17 -HSD2) and peroxisomal 17 -HSD4.. In order to elucidate the particular function of each of these two different enzymes, the human endometrial epithelial cell lines HEC-1-A and RL95–2 were examined with respect to the expression of 17 HSD isozymes.. They were compared with human endometrium in vivo. 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It shows increased peptidyl prolyl isomerase activity, the measured kcat/Km of 1.3 · 10 M 1 s is twofold greater than that of hFKBP12 and hFKBP51.. NMR studies of the interaction between FKB-6 and the C-terminal DAF-21 pentapeptide MEEVD show interactions consistent with those found between the large human immunophilin TPR domains and human Hsp90. Transformation Assay:Article Title: Cloning, expression and characterisation of FKB-6, the sole large TPR-containing immunophilin from C. elegans. Article Snippet: We have cloned, expressed, purified and characterised ceFKB-6, the only large tetratricopeptide repeat motif-containing immunophilin in Caenorhabditis elegans which is similar to the human orthologues FKBP51 and FKBP52.. It shows increased peptidyl prolyl isomerase activity, the measured kcat/Km of 1.3 · 10 M 1 s is twofold greater than that of hFKBP12 and hFKBP51.. NMR studies of the interaction between FKB-6 and the C-terminal DAF-21 pentapeptide MEEVD show interactions consistent with those found between the large human immunophilin TPR domains and human Hsp90. other:Article Title: A comprehensive expression analysis of the Arabidopsis proline-rich extensin-like receptor kinase gene family using bioinformatic and experimental approaches. Article Snippet: For AtPERK8-14, full coding region cDNAs were amplified by RT–PCR (Gibco BRL or Qiagen) and cloned into the |
