polymorphprep tm (lot: 00121) (Axis-Shield Diagnostics)

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Axis-Shield Diagnostics polymorphprep tm (lot: 00121)

Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of <t>Polymorphprep</t> TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

Polymorphprep Tm (Lot: 00121), supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymorphprep+tm/polymorphprep/pmc11719704-155-0-7
Average 90 stars, based on 1 article reviews

polymorphprep tm (lot: 00121) - by Bioz Stars, 2026-09

90/100 stars

Images

1) Product Images from "Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways"

Article Title: Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways

Journal: International Journal of Molecular Sciences

doi: 10.3390/ijms26010143

Figure Legend Snippet: Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

Techniques Used: Extraction, Isolation

Isolation:

Article Title: Tamm-Horsfall protein augments neutrophil NETosis during urinary tract infection
Article Snippet: .. Neutrophils were isolated using PolymorphPrep (Axis-Shield). ..

Article Title: Immune complexes-mediated activation of neutrophils in systemic lupus erythematosus is dependent on RNA recognition by toll-like receptor 8
Article Snippet: .. Neutrophils were isolated from healthy subjects with Polymorphprep (Axis-Shield, Dundee, UK) as described previously ( , ). .. Neutrophils were activated with R848 (InvivoGen, San Diego, CA, USA), CL075 (InvivoGen, San Diego, CA, USA), Imiquimod (InvivoGen, San Diego, CA, USA), or plasma (1:50 dilution) from SLE patients or HCs, for 2h, with or without prior addition of a TLR7/8 inhibitor (Example 438 from US 2019/0185469, provided by Gilead Sciences), a TLR7/8/9 inhibitor (Example 50 from WO 2019/238616, provided by Gilead Sciences), an IRAK4 inhibitor (provided by Gilead Sciences, Foster City, CA, USA), CU-CPT9a (TLR8 inhibitor, InvivoGen, San Diego, CA, USA), RNase A (Thermo Fisher Scientific, Waltham, MA, USA), FcγR II inhibitor (IV.3; Caprico Biotechnologies, Norcross, GA, USA) and hydroxychloroquine sulfate (HCQ, Sigma-Aldrich, Louis and Burlington, MA, USA) for 60 min. Activation was assessed by flow cytometer (Beckman Coulter, Brea, CA, USA) by assessing cell surface levels of CD66b (clone G10F5, BioLegend) and CD11b (clone CBRM1/5, BioLegend, San Diego, CA USA).

Article Title: The C-X-C Motif Chemokine Ligand 5, Which Exerts an Antioxidant Role by Inducing HO-1 Expression, Is C-X-C Motif Chemokine Receptor 2-Dependent in Human Prostate Stroma and Cancer Cells
Article Snippet: .. Neutrophils drawn from subjects’ peripheral blood were isolated by PolymorphPrep TM (Axis-Shield PoC, Oslo, Norway) provided by Dr. I-Hsien Lin, Department of Microbiology and Immunology, Chang Gung University, Taiwan. .. Neutrophils (1.5 × 10 6 ) were suspended in a 200 μL serum-free medium and added to the upper chamber of the transwell, with a pore size of 3.0 μm (PITP01250, Merck Millipore Ltd., Darmstadt, Germany).

other:

Article Title: Simvastatin ameliorates adverse pregnancy by inhibiting glycolysis-related NETs in obstetrical antiphospholipid syndrome.
Article Snippet: Fresh venous blood was drawn from normal pregnant women aged between 22 and 35 at 10–12 weeks of gestation, spread gently on PolymorphprepTM (Axis-Shield, Dundee, Scotland), and centrifuged at 750g for 35 min at room temperature.

Article Title: Extracellular traps in peripheral blood mononuclear cell fraction in childhood-onset systemic lupus erythematosus.
Article Snippet: Briefly, blood samples were mixed with Ficoll-Paque (Robbins Scientific Corporation, Sunnyvale, CA, USA) and PolymorphprepTM (Axis-Shield, Oslo, Norway) in a 1:1:1 ratio in a 50 mL-sterile tube and centrifuged at 1000 ×g for 30 min at room temperature without brake.

Article Title: Tumor-colonized Streptococcus mutans metabolically reprograms tumor microenvironment and promotes oral squamous cell carcinoma.
Article Snippet: Neutrophils were isolated from peripheral blood using PolymorphprepTM (AS1114683, Axis-Shield, Norway) density gradient centrifugation.

Saline:

Article Title: Whole-blood model reveals granulocytes as key sites of dengue virus propagation, expanding understanding of disease pathogenesis
Article Snippet: .. Packed cells remaining after PRP removal were diluted 1:1 with normal saline and centrifuged on Polymorphprep (Axis-Shield, Dundee, UK) to separate leukocytes from RBCs. .. Leukocytes were washed and stained with CD markers for sorting by FACSAria III (BD Biosciences, NJ, USA).

Review