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Accurate Chemical & Scientific Corporation polymorphprep tm
Polymorphprep Tm, supplied by Accurate Chemical & Scientific Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polymorphprep+tm/polymorphprep+tm/pmc03772210-245-10-12
Average 90 stars, based on 1 article reviews
polymorphprep tm - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Isolation:

Article Title: A Monoclonal Antibody (MCPR3-7) Interfering with the Activity of Proteinase 3 by an Allosteric Mechanism
Article Snippet: .. The neutrophils were isolated from EDTA-anticoagulated blood by centrifugation on Polymorphprep TM (Accurate Chemical and Scientific Corp., Westbury, NY) and hypotonic lysis of erythrocytes with distilled water. .. Cells were washed with cold Hanks' balanced salt solution without Ca 2+ /Mg 2+ (Mediatech Inc., Herndon, VA) and resuspended in Hanks' balanced salt solution with Ca 2+ /Mg 2+ (Mediatech Inc.) to obtain 1 × 10 7 cells/ml.

Centrifugation:

Article Title: A Monoclonal Antibody (MCPR3-7) Interfering with the Activity of Proteinase 3 by an Allosteric Mechanism
Article Snippet: .. The neutrophils were isolated from EDTA-anticoagulated blood by centrifugation on Polymorphprep TM (Accurate Chemical and Scientific Corp., Westbury, NY) and hypotonic lysis of erythrocytes with distilled water. .. Cells were washed with cold Hanks' balanced salt solution without Ca 2+ /Mg 2+ (Mediatech Inc., Herndon, VA) and resuspended in Hanks' balanced salt solution with Ca 2+ /Mg 2+ (Mediatech Inc.) to obtain 1 × 10 7 cells/ml.

Lysis:

Article Title: A Monoclonal Antibody (MCPR3-7) Interfering with the Activity of Proteinase 3 by an Allosteric Mechanism
Article Snippet: .. The neutrophils were isolated from EDTA-anticoagulated blood by centrifugation on Polymorphprep TM (Accurate Chemical and Scientific Corp., Westbury, NY) and hypotonic lysis of erythrocytes with distilled water. .. Cells were washed with cold Hanks' balanced salt solution without Ca 2+ /Mg 2+ (Mediatech Inc., Herndon, VA) and resuspended in Hanks' balanced salt solution with Ca 2+ /Mg 2+ (Mediatech Inc.) to obtain 1 × 10 7 cells/ml.



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Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of <t>Polymorphprep</t> TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.
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Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of <t>Polymorphprep</t> TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.
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Image Search Results


Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

Journal: International Journal of Molecular Sciences

Article Title: Neutrophil Extracellular Trap Formation Model Induced by Monosodium Urate and Phorbol Myristate Acetate: Involvement in MAPK Signaling Pathways

doi: 10.3390/ijms26010143

Figure Lengend Snippet: Extraction, isolation, and identification of human neutrophils: ( A ) Human peripheral blood was collected using an anticoagulant tube containing EDTA-K2, and human neutrophils were isolated in accordance with the instructions of Polymorphprep TM . The red arrows point to the neutrophil layer. ( B ) Cytometric markers and gating strategies were used to analyze the human neutrophil populations. (I) Cell debris was excluded using FSC-A/SSC-A plots. (II) The gate to exclude cell aggregates in the FSC-A/FSC-H diagram. (III) Neutrophils were identified using PE-CD16 and FITC-CD66b markers, achieving a purity of over 93%.

Article Snippet: PolymorphPrep TM (Lot: 00121) was purchased from Axis-Shield (Serumwerk Bernburg AG, Bernburg, Germany).

Techniques: Extraction, Isolation