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polymicrobial skin commensals standard  (ATCC)


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    ATCC polymicrobial skin commensals standard
    Polymicrobial Skin Commensals Standard, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 144 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polymicrobial/Mycobiome+Genomic%3B+DNA+Mix/pm37874143-253-1-5
    Average 96 stars, based on 144 article reviews
    polymicrobial skin commensals standard - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Purification:

    Article Title: Using carrier DNA in ultra-low input library preparations for next-generation sequencing
    Article Snippet: .. Three DNA templates were used for analysis in this study, including purified Enterobacteria phage lambda DNA (Lambda DNA; Oxford Nanopore Technologies, EXP-CTL001), ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, D6306), and Mycobiome Genomic DNA Mix (ATCC, MSA-1010). .. Prior to dilution, DNA concentrations were measured using Qubit fluorimetry (ThermoFisher Scientific).

    Lambda DNA Preparation:

    Article Title: Using carrier DNA in ultra-low input library preparations for next-generation sequencing
    Article Snippet: .. Three DNA templates were used for analysis in this study, including purified Enterobacteria phage lambda DNA (Lambda DNA; Oxford Nanopore Technologies, EXP-CTL001), ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, D6306), and Mycobiome Genomic DNA Mix (ATCC, MSA-1010). .. Prior to dilution, DNA concentrations were measured using Qubit fluorimetry (ThermoFisher Scientific).

    Quantitative Proteomics:

    Article Title: A Direct DNA Extraction Workflow for Metabarcoding Fungal Bioaerosols from Adhesive Samplers
    Article Snippet: Fungal DNA was quantified by qPCR targeting the 18S rRNA using the FungiQuant primers and probe set [ ] on a QuantStudio 7 Flex Real-Time PCR System (v1.7.2 software, Applied Biosystems, USA; ). qPCR was performed in technical triplicate for all samples, including standards, targets, no-template controls (NTCs), the kit control (KC), and the blank controls (UV and NoUV), to ensure consistency and reproducibility. .. Absolute quantification was performed using a standard curve generated from a 10-fold serial dilution (100–104 genome copy number) of fungal community genomic DNA (MSA-1010, ATCC, USA). ..

    Generated:

    Article Title: A Direct DNA Extraction Workflow for Metabarcoding Fungal Bioaerosols from Adhesive Samplers
    Article Snippet: Fungal DNA was quantified by qPCR targeting the 18S rRNA using the FungiQuant primers and probe set [ ] on a QuantStudio 7 Flex Real-Time PCR System (v1.7.2 software, Applied Biosystems, USA; ). qPCR was performed in technical triplicate for all samples, including standards, targets, no-template controls (NTCs), the kit control (KC), and the blank controls (UV and NoUV), to ensure consistency and reproducibility. .. Absolute quantification was performed using a standard curve generated from a 10-fold serial dilution (100–104 genome copy number) of fungal community genomic DNA (MSA-1010, ATCC, USA). ..

    Serial Dilution:

    Article Title: A Direct DNA Extraction Workflow for Metabarcoding Fungal Bioaerosols from Adhesive Samplers
    Article Snippet: Fungal DNA was quantified by qPCR targeting the 18S rRNA using the FungiQuant primers and probe set [ ] on a QuantStudio 7 Flex Real-Time PCR System (v1.7.2 software, Applied Biosystems, USA; ). qPCR was performed in technical triplicate for all samples, including standards, targets, no-template controls (NTCs), the kit control (KC), and the blank controls (UV and NoUV), to ensure consistency and reproducibility. .. Absolute quantification was performed using a standard curve generated from a 10-fold serial dilution (100–104 genome copy number) of fungal community genomic DNA (MSA-1010, ATCC, USA). ..

    Positive Control:

    Article Title: Contribution of endometrial microbiome to inflammation-mediated infertility in women undergoing ART.
    Article Snippet: .. A positive control consisting of a vaginal microbiome genome mix (NGS standard MSA-2007, ATCC) was also used. ..

    Article Title: Colibactin genes are highly prevalent in the developing infant gut microbiome.
    Article Snippet: An Agilent 4200 TapeStation system with High Sensitivity D5000 ScreenTape (Agilent Technologies, Inc.) was used to verify the quality and assess the final library size. .. A positive control (MSA-2002 20 Strain Even Mix Whole Cell Material (ATCC)) and a buffer extraction negative control were included. ..

    Article Title: Chicken vaccination reduces colonization and dissemination of Salmonella serovar Enteritidis with decreased susceptibility to ciprofloxacin.
    Article Snippet: .. Sterile, nuclease-free water was used as a negative control, and a mock community consisting of an even mix of the genomic material for 20 known bacterial strains (ATCC MSA-1002; ATCC, Manassas, VA) was used as a positive control. .. Successful amplification was confirmed by agarose gel electrophoresis and Qubit dsDNA High Sensitivity Quantitation Kit (Invitrogen).

    Next-Generation Sequencing:

    Article Title: Contribution of endometrial microbiome to inflammation-mediated infertility in women undergoing ART.
    Article Snippet: .. A positive control consisting of a vaginal microbiome genome mix (NGS standard MSA-2007, ATCC) was also used. ..

    Multiplex Assay:

    Article Title: Dynamic but constrained: repeated acquisitions of nutritional symbionts in bed bugs (Heteroptera: Cimicidae) from a narrow taxonomic pool
    Article Snippet: .. Using the QIAGEN Multiplex PCR Kit (Qiagen, Hilden, Germany), we amplified the V3–V4 region of the 16S rRNA gene from all samples, as well as from four blank (negative) PCR controls and two commercial gDNA (positive) controls (ATCC MSA-1000 and MSA-1001, each containing the same ten bacterial species in different proportions). .. Two-step PCR was performed with the 341F and 805R primers, which contained a staggered spacer and Illumina overhang adapters, followed by index PCR to add sample‐specific barcodes (Illumina 16S Metagenomic Sequencing Library Preparation Guide).

    Polymerase Chain Reaction:

    Article Title: Dynamic but constrained: repeated acquisitions of nutritional symbionts in bed bugs (Heteroptera: Cimicidae) from a narrow taxonomic pool
    Article Snippet: .. Using the QIAGEN Multiplex PCR Kit (Qiagen, Hilden, Germany), we amplified the V3–V4 region of the 16S rRNA gene from all samples, as well as from four blank (negative) PCR controls and two commercial gDNA (positive) controls (ATCC MSA-1000 and MSA-1001, each containing the same ten bacterial species in different proportions). .. Two-step PCR was performed with the 341F and 805R primers, which contained a staggered spacer and Illumina overhang adapters, followed by index PCR to add sample‐specific barcodes (Illumina 16S Metagenomic Sequencing Library Preparation Guide).

    Amplification:

    Article Title: Dynamic but constrained: repeated acquisitions of nutritional symbionts in bed bugs (Heteroptera: Cimicidae) from a narrow taxonomic pool
    Article Snippet: .. Using the QIAGEN Multiplex PCR Kit (Qiagen, Hilden, Germany), we amplified the V3–V4 region of the 16S rRNA gene from all samples, as well as from four blank (negative) PCR controls and two commercial gDNA (positive) controls (ATCC MSA-1000 and MSA-1001, each containing the same ten bacterial species in different proportions). .. Two-step PCR was performed with the 341F and 805R primers, which contained a staggered spacer and Illumina overhang adapters, followed by index PCR to add sample‐specific barcodes (Illumina 16S Metagenomic Sequencing Library Preparation Guide).

    Extraction:

    Article Title: Colibactin genes are highly prevalent in the developing infant gut microbiome.
    Article Snippet: An Agilent 4200 TapeStation system with High Sensitivity D5000 ScreenTape (Agilent Technologies, Inc.) was used to verify the quality and assess the final library size. .. A positive control (MSA-2002 20 Strain Even Mix Whole Cell Material (ATCC)) and a buffer extraction negative control were included. ..

    Negative Control:

    Article Title: Colibactin genes are highly prevalent in the developing infant gut microbiome.
    Article Snippet: An Agilent 4200 TapeStation system with High Sensitivity D5000 ScreenTape (Agilent Technologies, Inc.) was used to verify the quality and assess the final library size. .. A positive control (MSA-2002 20 Strain Even Mix Whole Cell Material (ATCC)) and a buffer extraction negative control were included. ..

    Article Title: Chicken vaccination reduces colonization and dissemination of Salmonella serovar Enteritidis with decreased susceptibility to ciprofloxacin.
    Article Snippet: .. Sterile, nuclease-free water was used as a negative control, and a mock community consisting of an even mix of the genomic material for 20 known bacterial strains (ATCC MSA-1002; ATCC, Manassas, VA) was used as a positive control. .. Successful amplification was confirmed by agarose gel electrophoresis and Qubit dsDNA High Sensitivity Quantitation Kit (Invitrogen).

    DNA Extraction:

    Article Title: Critical assessment of intratumor and low-biomass microbiome using long-read sequencing
    Article Snippet: .. Using the dissected mice tissue samples, brain and lung from #1 mice and stomach from the #2 mice were spiked with a mock bacterial mixture consisting of 20 equally abundant strains (MSA-2002; ATCC) onto each tissue sample prior to DNA isolation. ..

    Sterility:

    Article Title: Chicken vaccination reduces colonization and dissemination of Salmonella serovar Enteritidis with decreased susceptibility to ciprofloxacin.
    Article Snippet: .. Sterile, nuclease-free water was used as a negative control, and a mock community consisting of an even mix of the genomic material for 20 known bacterial strains (ATCC MSA-1002; ATCC, Manassas, VA) was used as a positive control. .. Successful amplification was confirmed by agarose gel electrophoresis and Qubit dsDNA High Sensitivity Quantitation Kit (Invitrogen).



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