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Structured Review

Genechem mcol1a2 sv40 polya
Mcol1a2 Sv40 Polya, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polya/1a+a+a+a+a+chemicals+genechem+genechem+genechem+genechem+gfp+lenti+lenti+lenti+mcherry+mitodsred2+n+n+n+n+pgc+shrna+strains+virus/pmc13265906-27-0-3
Average 86 stars, based on 1 article reviews
mcol1a2 sv40 polya - by Bioz Stars, 2026-11
86/100 stars

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Related Articles

Construct:

Article Title: P2X7R deficiency alleviates cardiac senescence by enhancing mitophagy via the HuR/TRIM26/NR4A1 axis
Article Snippet: .. The constructs included AAV9‐cTnT encoding an empty vector (EV), P2X7R (AAV9‐cTnT‐P2X7R oe ) or NR4A1 (AAV9‐cTnT‐NR4A1 oe ) (GV571; Genechem Co. Ltd.). ..

Ubiquitin Proteomics:

Article Title: LRP1 activated by AT2 cell-secreted MDK inhibits fibrotic ferroptosis in idiopathic pulmonary fibrosis
Article Snippet: .. Antibodies CTHRC1 Novus Cat#81744; RRID: AB_3414573 PDPN DSHB Cat# 8.1.1; RRID: AB_531893 SFTPC Merck Cat#ABC99; RRID: AB_2755001 ACSL4 CST Cat#9189 SLC7A11 CST Cat#12691; RRID: AB_2687474 GPX4 abcam Cat#ab125066; RRID: AB_10973901 FTH1 CST Cat#4393; RRID: AB_11217441 Beta-actin CST Cat#3700 LRP1 Invitrogen Cat#MA1-80192; RRID: AB_929727 MDK Invitrogen Cat#MA5-32538; RRID: AB_2809815 OTUB1 OriGene Cat#TA505150; RRID: AB_2622874 AKT Proteintech Cat#10176-2-AP; RRID: AB_2224574 p-AKT Proteintech Cat#66444-1-Ig; RRID: AB_2782958 p38 Proteintech Cat#14064-1-AP; RRID: AB_2878007 p-p38 Proteintech Cat#81212-2-RR; RRID: AB_3743373 Ubiquitin Invitrogen Cat#14-6078-82; RRID: AB_837154 Myc-Tag CST Cat#2276 FLAG Tag Sigma-Aldrich Cat#F3165; RRID: AB_259529 GST-Tag CST Cat#2624; RRID: AB_2189875 His-Tag CST Cat#2366; RRID: AB_2115719 HA-Tag CST Cat3724; RRID: AB_1549585 Bacterial and virus strains pAAV-SP-Cp-SV40 PolyA Genechem GCNV0402707 mCOL1A2-SV40 PolyA Genechem GCNV0402708 Chemicals, peptides, and recombinant proteins Bleomycin MedChemExpress HY-17565A PBS Gibico 20012027 4% paraformaldehyde Beyotime P0099 citrate buffer (pH 6.0) Thermo Fisher Scientific 005000 HRP-labelled goat anti-mouse/rabbit IgG abcam ab6721 DMEM Thermo Fisher Scientific 11965 FBS Thermo Fisher Scientific 10099141c Antibiotic-antimycotic Thermo Fisher Scientific 15240062 BODIPYTM 581/591 C11 fluorescence probe Thermo Fisher Scientific D3861 RIPA Lysis Buffer Beyotime P0013B Immunoprecipitation (IP) lysis buffer Thermo Fisher Scientific 87787 protein A/G-beads Thermo Fisher Scientific 88802 Critical commercial assays Hydroxyproline Assay Kit (Colorimetric) abcam ab222941 Human MDK ELISA Kit abcam ab193761 Mouse MDK ELISA Kit abcam ab279416 Masson’s trichrome staining Kit Solarbio G1340 (Continued on next page) iScience 29, 116212, June 19, 2026 e1 ..

FLAG-tag:

Article Title: LRP1 activated by AT2 cell-secreted MDK inhibits fibrotic ferroptosis in idiopathic pulmonary fibrosis
Article Snippet: .. Antibodies CTHRC1 Novus Cat#81744; RRID: AB_3414573 PDPN DSHB Cat# 8.1.1; RRID: AB_531893 SFTPC Merck Cat#ABC99; RRID: AB_2755001 ACSL4 CST Cat#9189 SLC7A11 CST Cat#12691; RRID: AB_2687474 GPX4 abcam Cat#ab125066; RRID: AB_10973901 FTH1 CST Cat#4393; RRID: AB_11217441 Beta-actin CST Cat#3700 LRP1 Invitrogen Cat#MA1-80192; RRID: AB_929727 MDK Invitrogen Cat#MA5-32538; RRID: AB_2809815 OTUB1 OriGene Cat#TA505150; RRID: AB_2622874 AKT Proteintech Cat#10176-2-AP; RRID: AB_2224574 p-AKT Proteintech Cat#66444-1-Ig; RRID: AB_2782958 p38 Proteintech Cat#14064-1-AP; RRID: AB_2878007 p-p38 Proteintech Cat#81212-2-RR; RRID: AB_3743373 Ubiquitin Invitrogen Cat#14-6078-82; RRID: AB_837154 Myc-Tag CST Cat#2276 FLAG Tag Sigma-Aldrich Cat#F3165; RRID: AB_259529 GST-Tag CST Cat#2624; RRID: AB_2189875 His-Tag CST Cat#2366; RRID: AB_2115719 HA-Tag CST Cat3724; RRID: AB_1549585 Bacterial and virus strains pAAV-SP-Cp-SV40 PolyA Genechem GCNV0402707 mCOL1A2-SV40 PolyA Genechem GCNV0402708 Chemicals, peptides, and recombinant proteins Bleomycin MedChemExpress HY-17565A PBS Gibico 20012027 4% paraformaldehyde Beyotime P0099 citrate buffer (pH 6.0) Thermo Fisher Scientific 005000 HRP-labelled goat anti-mouse/rabbit IgG abcam ab6721 DMEM Thermo Fisher Scientific 11965 FBS Thermo Fisher Scientific 10099141c Antibiotic-antimycotic Thermo Fisher Scientific 15240062 BODIPYTM 581/591 C11 fluorescence probe Thermo Fisher Scientific D3861 RIPA Lysis Buffer Beyotime P0013B Immunoprecipitation (IP) lysis buffer Thermo Fisher Scientific 87787 protein A/G-beads Thermo Fisher Scientific 88802 Critical commercial assays Hydroxyproline Assay Kit (Colorimetric) abcam ab222941 Human MDK ELISA Kit abcam ab193761 Mouse MDK ELISA Kit abcam ab279416 Masson’s trichrome staining Kit Solarbio G1340 (Continued on next page) iScience 29, 116212, June 19, 2026 e1 ..

Virus:

Article Title: LRP1 activated by AT2 cell-secreted MDK inhibits fibrotic ferroptosis in idiopathic pulmonary fibrosis
Article Snippet: .. Antibodies CTHRC1 Novus Cat#81744; RRID: AB_3414573 PDPN DSHB Cat# 8.1.1; RRID: AB_531893 SFTPC Merck Cat#ABC99; RRID: AB_2755001 ACSL4 CST Cat#9189 SLC7A11 CST Cat#12691; RRID: AB_2687474 GPX4 abcam Cat#ab125066; RRID: AB_10973901 FTH1 CST Cat#4393; RRID: AB_11217441 Beta-actin CST Cat#3700 LRP1 Invitrogen Cat#MA1-80192; RRID: AB_929727 MDK Invitrogen Cat#MA5-32538; RRID: AB_2809815 OTUB1 OriGene Cat#TA505150; RRID: AB_2622874 AKT Proteintech Cat#10176-2-AP; RRID: AB_2224574 p-AKT Proteintech Cat#66444-1-Ig; RRID: AB_2782958 p38 Proteintech Cat#14064-1-AP; RRID: AB_2878007 p-p38 Proteintech Cat#81212-2-RR; RRID: AB_3743373 Ubiquitin Invitrogen Cat#14-6078-82; RRID: AB_837154 Myc-Tag CST Cat#2276 FLAG Tag Sigma-Aldrich Cat#F3165; RRID: AB_259529 GST-Tag CST Cat#2624; RRID: AB_2189875 His-Tag CST Cat#2366; RRID: AB_2115719 HA-Tag CST Cat3724; RRID: AB_1549585 Bacterial and virus strains pAAV-SP-Cp-SV40 PolyA Genechem GCNV0402707 mCOL1A2-SV40 PolyA Genechem GCNV0402708 Chemicals, peptides, and recombinant proteins Bleomycin MedChemExpress HY-17565A PBS Gibico 20012027 4% paraformaldehyde Beyotime P0099 citrate buffer (pH 6.0) Thermo Fisher Scientific 005000 HRP-labelled goat anti-mouse/rabbit IgG abcam ab6721 DMEM Thermo Fisher Scientific 11965 FBS Thermo Fisher Scientific 10099141c Antibiotic-antimycotic Thermo Fisher Scientific 15240062 BODIPYTM 581/591 C11 fluorescence probe Thermo Fisher Scientific D3861 RIPA Lysis Buffer Beyotime P0013B Immunoprecipitation (IP) lysis buffer Thermo Fisher Scientific 87787 protein A/G-beads Thermo Fisher Scientific 88802 Critical commercial assays Hydroxyproline Assay Kit (Colorimetric) abcam ab222941 Human MDK ELISA Kit abcam ab193761 Mouse MDK ELISA Kit abcam ab279416 Masson’s trichrome staining Kit Solarbio G1340 (Continued on next page) iScience 29, 116212, June 19, 2026 e1 ..

Article Title: A preclinical candidate of cyclophilin D inhibition improves alcohol-associated liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mitofusin 1 antibody Abcam Cat#ab221661; RRID:AB_2941083 Anti-Mitofusin 2 antibody Abcam Cat#ab124773; RRID:AB_10999860 Anti-Cytochrome C antibody Abcam Cat#ab133504; RRID:AB_2802115 Anti-Nrf2 antibody Abcam Cat#ab92946; RRID:AB_10561604 Anti-SOD1 antibody Abcam Cat#ab16831; RRID:AB_302535 Anti-Catalase antibody Abcam Cat#ab52477; RRID:AB_868694 Anti-CYP2E1 antibody Abcam Cat#ab28146; RRID:AB_2089985 Anti-DRP1 antibody Cell Signaling Technology Cat#8570; RRID:AB_10950498 Anti-FIS1 antibody Cell Signaling Technology Cat#32525; RRID:AB_2927422 Anti-OPA1 antibody Cell Signaling Technology Cat#80471; RRID:AB_2734117 Anti-Bcl-2 antibody Cell Signaling Technology Cat#3498; RRID:AB_1903907 Anti-Bax antibody Cell Signaling Technology Cat#2772; RRID:AB_10695870 Anti-PCNA antibody Cell Signaling Technology Cat#13110; RRID:AB_2636979 Anti- iNOS antibody Cell Signaling Technology Cat#13120; RRID:AB_2687529 Anti-Cleaved Caspase-3 antibody Cell Signaling Technology Cat#9661; RRID:AB_2341188 Anti-NF-kappaB p65 antibody Cell Signaling Technology Cat#8242; RRID:AB_10859369 Anti-Phospho-NF-kappaB p65 antibody Cell Signaling Technology Cat#3033; RRID:AB_331284 Anti-GAPDH antibody Cell Signaling Technology Cat#2118; RRID:AB_561053 Anti-Phospho-Nrf2 antibody Affinity Biosciences CatDF7519 Bacterial and virus strains AAV8-ApoE/hAATp-EGFP-FT2ANM_134084-3Flag-SV40 PolyA GeneChem N/A AAV8-hAAT-shPpia-EGFP GeneChem N/A AAV8-hAAT-shPpib-EGFP GeneChem N/A Biological samples Healthy/ALD adult liver tissue This paper N/A Chemicals, peptides, and recombinant proteins RN-0001 Farsight Medical Technology N/A Debio-025 MedChemExpress Cat#HY-12559 CRV431 MedChemExpress Cat#HY-135644 Critical commercial assays Cytotoxicity LDH Assay Kit MedChemExpress Cat#HY-K1090 Apoptosis detection kit Vazyme Cat#A211-01 Calcineurin phosphatase assay kit Enzo Life Sciences Cat#BML-AK804-0001 Calcium colorimetric assay kit Beyotime Biotechnology Cat#S1063S Mitochondria isolation kit MedChemExpress Cat#HY-K1061 ATP/ADP ratio assay kit Abcam Cat#ab65313 Triglyceride content detection kit Solarbio Cat#BC0625 Non-esterified FFA assay kit Elabscience Cat#E-BC-K013 Deposited data RN-0001 This paper PubChem CID: 146639799 RNA-seq data of mouse liver This paper GEO: GSE316128 Single-cell RNA-seq data of human liver MacParland et al.54 GEO: GSE115469 (Continued on next page) e1 Cell Reports Medicine 7, 102654, March 17, 2026 ..

Article Title: Non-genetically reprogrammed meta-neutrophils potentiate chemo-immunotherapy against lung metastatic triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse Ly-6G Antibody(Alexa Fluro 488) BioLegend (USA) Cat# 127626; RRID: AB_2561340 Anti-mouse/human CD11b Antibody (APC) BioLegend (USA) Cat# 101211; RRID: AB_312794 Anti-mouseTNF-α Antibody (PE) BioLegend (USA) Cat# 506306; RRID: AB_315427 Anti-mouse CD8a Antibody (APC/ Cyanine7) BioLegend (USA) Cat# 100714; RRID: AB_312753 Anti-mouseCD49b Antibody (Pacific BlueTM) BioLegend (USA) Cat# 108917; RRID: AB_2249376 Anti-mouse IFN-γ Antibody Antibody BioLegend (USA) Cat# 505810; RRID: AB_315404 Anti-human/mouse Granzyme B Antibody (FITC) BioLegend (USA) Cat# 515403; RRID: AB_2114575 Anti-mouse CD80 Antibody (FITC) BioLegend (USA) Cat# 104706; RRID: AB_313127 Anti-mouse CD86 Antibody ((Alexa Fluor 647) BioLegend (USA) Cat# 105019; RRID: AB_493465 Anti-mouse ICAM-1 Antibody (PE) Invitrogen (USA) Cat# 12-0541-81; RRID: AB_465706 Anti-mouse MHCII Antibody (PE) BioLegend (USA) Cat# 116407; RRID: AB_313726 Anti-mouse CD45 Antibody (PE) BioLegend (USA) Cat# 147711; RRID: AB_2563597 Anti-mouse F4/80 Antibody (APC) BioLegend (USA) Cat# 123115; RRID: AB_893493 Anti-mouse CD11c Antibody (FITC) BioLegend (USA) Cat# 117305; RRID: AB_313774 Anti-Mouse CD3 SAFIRE Purified BioGem (USA) Cat# 05112-25; RRID: AB_3099697 Anti-Mouse CD28 SAFIRE Purified BioGem (USA) Cat# 10312-25; RRID: AB_3099698 Bacterial and virus strains Firefly Luciferase-mCherry Lentivirus GeneChem (China) N/A mCherry expressing lentivirus GeneChem (China) N/A Cell lines 4T1 ATCC N/A EMT6 ATCC N/A HUVEC ATCC N/A MDA-MB-231 ATCC N/A Chemicals, peptides, and recombinant proteins RPMI1640 HyClone (USA) N/A DMEM HyClone (USA) N/A Fetal bovine serum HyClone (USA) N/A ACK lysis buffer Haoyang (China) N/A PBS HyClone (USA) N/A 10xPBS Solarbio (China) Cat# P1022 Percoll HyClone (USA) Cat# 17-0819-09 Recombinant mouse IFNγ Prospec-Tany Cat# cyt-358 Recombinant human IFNγ Thermo (USA) Cat# 300-02-100UG RNA isolater Total RNA Extraction Reagent Vazyme (China) Cat# R401-01 HiScript III RT SuperMix for qPCR Vazyme (China) Cat# R323-01 Hieff qPCR SYBR Green Master Mix YEASEN (China) Cat# 11201ES03 Ionomycin YEASEN (China) Cat# 50402ES03 PMA Sigma-Aldrich Cat# P1585 Brefeldin A Beyotime (China) Cat# S1536 (Continued on next page) e1 Cell Reports Medicine 7, 102868, July 21, 2026 Article ll OPEN ACCESS .. The murine triple-negative breast cancer cells (4T1 and EMT6), human umbilical vein endothelial cells (HUVEC), and human triplenegative breast cancer cells (MDA-MB-231) were purchased from the American Type Culture Collection (ATCC).

Recombinant:

Article Title: LRP1 activated by AT2 cell-secreted MDK inhibits fibrotic ferroptosis in idiopathic pulmonary fibrosis
Article Snippet: .. Antibodies CTHRC1 Novus Cat#81744; RRID: AB_3414573 PDPN DSHB Cat# 8.1.1; RRID: AB_531893 SFTPC Merck Cat#ABC99; RRID: AB_2755001 ACSL4 CST Cat#9189 SLC7A11 CST Cat#12691; RRID: AB_2687474 GPX4 abcam Cat#ab125066; RRID: AB_10973901 FTH1 CST Cat#4393; RRID: AB_11217441 Beta-actin CST Cat#3700 LRP1 Invitrogen Cat#MA1-80192; RRID: AB_929727 MDK Invitrogen Cat#MA5-32538; RRID: AB_2809815 OTUB1 OriGene Cat#TA505150; RRID: AB_2622874 AKT Proteintech Cat#10176-2-AP; RRID: AB_2224574 p-AKT Proteintech Cat#66444-1-Ig; RRID: AB_2782958 p38 Proteintech Cat#14064-1-AP; RRID: AB_2878007 p-p38 Proteintech Cat#81212-2-RR; RRID: AB_3743373 Ubiquitin Invitrogen Cat#14-6078-82; RRID: AB_837154 Myc-Tag CST Cat#2276 FLAG Tag Sigma-Aldrich Cat#F3165; RRID: AB_259529 GST-Tag CST Cat#2624; RRID: AB_2189875 His-Tag CST Cat#2366; RRID: AB_2115719 HA-Tag CST Cat3724; RRID: AB_1549585 Bacterial and virus strains pAAV-SP-Cp-SV40 PolyA Genechem GCNV0402707 mCOL1A2-SV40 PolyA Genechem GCNV0402708 Chemicals, peptides, and recombinant proteins Bleomycin MedChemExpress HY-17565A PBS Gibico 20012027 4% paraformaldehyde Beyotime P0099 citrate buffer (pH 6.0) Thermo Fisher Scientific 005000 HRP-labelled goat anti-mouse/rabbit IgG abcam ab6721 DMEM Thermo Fisher Scientific 11965 FBS Thermo Fisher Scientific 10099141c Antibiotic-antimycotic Thermo Fisher Scientific 15240062 BODIPYTM 581/591 C11 fluorescence probe Thermo Fisher Scientific D3861 RIPA Lysis Buffer Beyotime P0013B Immunoprecipitation (IP) lysis buffer Thermo Fisher Scientific 87787 protein A/G-beads Thermo Fisher Scientific 88802 Critical commercial assays Hydroxyproline Assay Kit (Colorimetric) abcam ab222941 Human MDK ELISA Kit abcam ab193761 Mouse MDK ELISA Kit abcam ab279416 Masson’s trichrome staining Kit Solarbio G1340 (Continued on next page) iScience 29, 116212, June 19, 2026 e1 ..

Article Title: A preclinical candidate of cyclophilin D inhibition improves alcohol-associated liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mitofusin 1 antibody Abcam Cat#ab221661; RRID:AB_2941083 Anti-Mitofusin 2 antibody Abcam Cat#ab124773; RRID:AB_10999860 Anti-Cytochrome C antibody Abcam Cat#ab133504; RRID:AB_2802115 Anti-Nrf2 antibody Abcam Cat#ab92946; RRID:AB_10561604 Anti-SOD1 antibody Abcam Cat#ab16831; RRID:AB_302535 Anti-Catalase antibody Abcam Cat#ab52477; RRID:AB_868694 Anti-CYP2E1 antibody Abcam Cat#ab28146; RRID:AB_2089985 Anti-DRP1 antibody Cell Signaling Technology Cat#8570; RRID:AB_10950498 Anti-FIS1 antibody Cell Signaling Technology Cat#32525; RRID:AB_2927422 Anti-OPA1 antibody Cell Signaling Technology Cat#80471; RRID:AB_2734117 Anti-Bcl-2 antibody Cell Signaling Technology Cat#3498; RRID:AB_1903907 Anti-Bax antibody Cell Signaling Technology Cat#2772; RRID:AB_10695870 Anti-PCNA antibody Cell Signaling Technology Cat#13110; RRID:AB_2636979 Anti- iNOS antibody Cell Signaling Technology Cat#13120; RRID:AB_2687529 Anti-Cleaved Caspase-3 antibody Cell Signaling Technology Cat#9661; RRID:AB_2341188 Anti-NF-kappaB p65 antibody Cell Signaling Technology Cat#8242; RRID:AB_10859369 Anti-Phospho-NF-kappaB p65 antibody Cell Signaling Technology Cat#3033; RRID:AB_331284 Anti-GAPDH antibody Cell Signaling Technology Cat#2118; RRID:AB_561053 Anti-Phospho-Nrf2 antibody Affinity Biosciences CatDF7519 Bacterial and virus strains AAV8-ApoE/hAATp-EGFP-FT2ANM_134084-3Flag-SV40 PolyA GeneChem N/A AAV8-hAAT-shPpia-EGFP GeneChem N/A AAV8-hAAT-shPpib-EGFP GeneChem N/A Biological samples Healthy/ALD adult liver tissue This paper N/A Chemicals, peptides, and recombinant proteins RN-0001 Farsight Medical Technology N/A Debio-025 MedChemExpress Cat#HY-12559 CRV431 MedChemExpress Cat#HY-135644 Critical commercial assays Cytotoxicity LDH Assay Kit MedChemExpress Cat#HY-K1090 Apoptosis detection kit Vazyme Cat#A211-01 Calcineurin phosphatase assay kit Enzo Life Sciences Cat#BML-AK804-0001 Calcium colorimetric assay kit Beyotime Biotechnology Cat#S1063S Mitochondria isolation kit MedChemExpress Cat#HY-K1061 ATP/ADP ratio assay kit Abcam Cat#ab65313 Triglyceride content detection kit Solarbio Cat#BC0625 Non-esterified FFA assay kit Elabscience Cat#E-BC-K013 Deposited data RN-0001 This paper PubChem CID: 146639799 RNA-seq data of mouse liver This paper GEO: GSE316128 Single-cell RNA-seq data of human liver MacParland et al.54 GEO: GSE115469 (Continued on next page) e1 Cell Reports Medicine 7, 102654, March 17, 2026 ..

Article Title: Non-genetically reprogrammed meta-neutrophils potentiate chemo-immunotherapy against lung metastatic triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse Ly-6G Antibody(Alexa Fluro 488) BioLegend (USA) Cat# 127626; RRID: AB_2561340 Anti-mouse/human CD11b Antibody (APC) BioLegend (USA) Cat# 101211; RRID: AB_312794 Anti-mouseTNF-α Antibody (PE) BioLegend (USA) Cat# 506306; RRID: AB_315427 Anti-mouse CD8a Antibody (APC/ Cyanine7) BioLegend (USA) Cat# 100714; RRID: AB_312753 Anti-mouseCD49b Antibody (Pacific BlueTM) BioLegend (USA) Cat# 108917; RRID: AB_2249376 Anti-mouse IFN-γ Antibody Antibody BioLegend (USA) Cat# 505810; RRID: AB_315404 Anti-human/mouse Granzyme B Antibody (FITC) BioLegend (USA) Cat# 515403; RRID: AB_2114575 Anti-mouse CD80 Antibody (FITC) BioLegend (USA) Cat# 104706; RRID: AB_313127 Anti-mouse CD86 Antibody ((Alexa Fluor 647) BioLegend (USA) Cat# 105019; RRID: AB_493465 Anti-mouse ICAM-1 Antibody (PE) Invitrogen (USA) Cat# 12-0541-81; RRID: AB_465706 Anti-mouse MHCII Antibody (PE) BioLegend (USA) Cat# 116407; RRID: AB_313726 Anti-mouse CD45 Antibody (PE) BioLegend (USA) Cat# 147711; RRID: AB_2563597 Anti-mouse F4/80 Antibody (APC) BioLegend (USA) Cat# 123115; RRID: AB_893493 Anti-mouse CD11c Antibody (FITC) BioLegend (USA) Cat# 117305; RRID: AB_313774 Anti-Mouse CD3 SAFIRE Purified BioGem (USA) Cat# 05112-25; RRID: AB_3099697 Anti-Mouse CD28 SAFIRE Purified BioGem (USA) Cat# 10312-25; RRID: AB_3099698 Bacterial and virus strains Firefly Luciferase-mCherry Lentivirus GeneChem (China) N/A mCherry expressing lentivirus GeneChem (China) N/A Cell lines 4T1 ATCC N/A EMT6 ATCC N/A HUVEC ATCC N/A MDA-MB-231 ATCC N/A Chemicals, peptides, and recombinant proteins RPMI1640 HyClone (USA) N/A DMEM HyClone (USA) N/A Fetal bovine serum HyClone (USA) N/A ACK lysis buffer Haoyang (China) N/A PBS HyClone (USA) N/A 10xPBS Solarbio (China) Cat# P1022 Percoll HyClone (USA) Cat# 17-0819-09 Recombinant mouse IFNγ Prospec-Tany Cat# cyt-358 Recombinant human IFNγ Thermo (USA) Cat# 300-02-100UG RNA isolater Total RNA Extraction Reagent Vazyme (China) Cat# R401-01 HiScript III RT SuperMix for qPCR Vazyme (China) Cat# R323-01 Hieff qPCR SYBR Green Master Mix YEASEN (China) Cat# 11201ES03 Ionomycin YEASEN (China) Cat# 50402ES03 PMA Sigma-Aldrich Cat# P1585 Brefeldin A Beyotime (China) Cat# S1536 (Continued on next page) e1 Cell Reports Medicine 7, 102868, July 21, 2026 Article ll OPEN ACCESS .. The murine triple-negative breast cancer cells (4T1 and EMT6), human umbilical vein endothelial cells (HUVEC), and human triplenegative breast cancer cells (MDA-MB-231) were purchased from the American Type Culture Collection (ATCC).

Fluorescence:

Article Title: LRP1 activated by AT2 cell-secreted MDK inhibits fibrotic ferroptosis in idiopathic pulmonary fibrosis
Article Snippet: .. Antibodies CTHRC1 Novus Cat#81744; RRID: AB_3414573 PDPN DSHB Cat# 8.1.1; RRID: AB_531893 SFTPC Merck Cat#ABC99; RRID: AB_2755001 ACSL4 CST Cat#9189 SLC7A11 CST Cat#12691; RRID: AB_2687474 GPX4 abcam Cat#ab125066; RRID: AB_10973901 FTH1 CST Cat#4393; RRID: AB_11217441 Beta-actin CST Cat#3700 LRP1 Invitrogen Cat#MA1-80192; RRID: AB_929727 MDK Invitrogen Cat#MA5-32538; RRID: AB_2809815 OTUB1 OriGene Cat#TA505150; RRID: AB_2622874 AKT Proteintech Cat#10176-2-AP; RRID: AB_2224574 p-AKT Proteintech Cat#66444-1-Ig; RRID: AB_2782958 p38 Proteintech Cat#14064-1-AP; RRID: AB_2878007 p-p38 Proteintech Cat#81212-2-RR; RRID: AB_3743373 Ubiquitin Invitrogen Cat#14-6078-82; RRID: AB_837154 Myc-Tag CST Cat#2276 FLAG Tag Sigma-Aldrich Cat#F3165; RRID: AB_259529 GST-Tag CST Cat#2624; RRID: AB_2189875 His-Tag CST Cat#2366; RRID: AB_2115719 HA-Tag CST Cat3724; RRID: AB_1549585 Bacterial and virus strains pAAV-SP-Cp-SV40 PolyA Genechem GCNV0402707 mCOL1A2-SV40 PolyA Genechem GCNV0402708 Chemicals, peptides, and recombinant proteins Bleomycin MedChemExpress HY-17565A PBS Gibico 20012027 4% paraformaldehyde Beyotime P0099 citrate buffer (pH 6.0) Thermo Fisher Scientific 005000 HRP-labelled goat anti-mouse/rabbit IgG abcam ab6721 DMEM Thermo Fisher Scientific 11965 FBS Thermo Fisher Scientific 10099141c Antibiotic-antimycotic Thermo Fisher Scientific 15240062 BODIPYTM 581/591 C11 fluorescence probe Thermo Fisher Scientific D3861 RIPA Lysis Buffer Beyotime P0013B Immunoprecipitation (IP) lysis buffer Thermo Fisher Scientific 87787 protein A/G-beads Thermo Fisher Scientific 88802 Critical commercial assays Hydroxyproline Assay Kit (Colorimetric) abcam ab222941 Human MDK ELISA Kit abcam ab193761 Mouse MDK ELISA Kit abcam ab279416 Masson’s trichrome staining Kit Solarbio G1340 (Continued on next page) iScience 29, 116212, June 19, 2026 e1 ..

Lysis:

Article Title: LRP1 activated by AT2 cell-secreted MDK inhibits fibrotic ferroptosis in idiopathic pulmonary fibrosis
Article Snippet: .. Antibodies CTHRC1 Novus Cat#81744; RRID: AB_3414573 PDPN DSHB Cat# 8.1.1; RRID: AB_531893 SFTPC Merck Cat#ABC99; RRID: AB_2755001 ACSL4 CST Cat#9189 SLC7A11 CST Cat#12691; RRID: AB_2687474 GPX4 abcam Cat#ab125066; RRID: AB_10973901 FTH1 CST Cat#4393; RRID: AB_11217441 Beta-actin CST Cat#3700 LRP1 Invitrogen Cat#MA1-80192; RRID: AB_929727 MDK Invitrogen Cat#MA5-32538; RRID: AB_2809815 OTUB1 OriGene Cat#TA505150; RRID: AB_2622874 AKT Proteintech Cat#10176-2-AP; RRID: AB_2224574 p-AKT Proteintech Cat#66444-1-Ig; RRID: AB_2782958 p38 Proteintech Cat#14064-1-AP; RRID: AB_2878007 p-p38 Proteintech Cat#81212-2-RR; RRID: AB_3743373 Ubiquitin Invitrogen Cat#14-6078-82; RRID: AB_837154 Myc-Tag CST Cat#2276 FLAG Tag Sigma-Aldrich Cat#F3165; RRID: AB_259529 GST-Tag CST Cat#2624; RRID: AB_2189875 His-Tag CST Cat#2366; RRID: AB_2115719 HA-Tag CST Cat3724; RRID: AB_1549585 Bacterial and virus strains pAAV-SP-Cp-SV40 PolyA Genechem GCNV0402707 mCOL1A2-SV40 PolyA Genechem GCNV0402708 Chemicals, peptides, and recombinant proteins Bleomycin MedChemExpress HY-17565A PBS Gibico 20012027 4% paraformaldehyde Beyotime P0099 citrate buffer (pH 6.0) Thermo Fisher Scientific 005000 HRP-labelled goat anti-mouse/rabbit IgG abcam ab6721 DMEM Thermo Fisher Scientific 11965 FBS Thermo Fisher Scientific 10099141c Antibiotic-antimycotic Thermo Fisher Scientific 15240062 BODIPYTM 581/591 C11 fluorescence probe Thermo Fisher Scientific D3861 RIPA Lysis Buffer Beyotime P0013B Immunoprecipitation (IP) lysis buffer Thermo Fisher Scientific 87787 protein A/G-beads Thermo Fisher Scientific 88802 Critical commercial assays Hydroxyproline Assay Kit (Colorimetric) abcam ab222941 Human MDK ELISA Kit abcam ab193761 Mouse MDK ELISA Kit abcam ab279416 Masson’s trichrome staining Kit Solarbio G1340 (Continued on next page) iScience 29, 116212, June 19, 2026 e1 ..

Article Title: Non-genetically reprogrammed meta-neutrophils potentiate chemo-immunotherapy against lung metastatic triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse Ly-6G Antibody(Alexa Fluro 488) BioLegend (USA) Cat# 127626; RRID: AB_2561340 Anti-mouse/human CD11b Antibody (APC) BioLegend (USA) Cat# 101211; RRID: AB_312794 Anti-mouseTNF-α Antibody (PE) BioLegend (USA) Cat# 506306; RRID: AB_315427 Anti-mouse CD8a Antibody (APC/ Cyanine7) BioLegend (USA) Cat# 100714; RRID: AB_312753 Anti-mouseCD49b Antibody (Pacific BlueTM) BioLegend (USA) Cat# 108917; RRID: AB_2249376 Anti-mouse IFN-γ Antibody Antibody BioLegend (USA) Cat# 505810; RRID: AB_315404 Anti-human/mouse Granzyme B Antibody (FITC) BioLegend (USA) Cat# 515403; RRID: AB_2114575 Anti-mouse CD80 Antibody (FITC) BioLegend (USA) Cat# 104706; RRID: AB_313127 Anti-mouse CD86 Antibody ((Alexa Fluor 647) BioLegend (USA) Cat# 105019; RRID: AB_493465 Anti-mouse ICAM-1 Antibody (PE) Invitrogen (USA) Cat# 12-0541-81; RRID: AB_465706 Anti-mouse MHCII Antibody (PE) BioLegend (USA) Cat# 116407; RRID: AB_313726 Anti-mouse CD45 Antibody (PE) BioLegend (USA) Cat# 147711; RRID: AB_2563597 Anti-mouse F4/80 Antibody (APC) BioLegend (USA) Cat# 123115; RRID: AB_893493 Anti-mouse CD11c Antibody (FITC) BioLegend (USA) Cat# 117305; RRID: AB_313774 Anti-Mouse CD3 SAFIRE Purified BioGem (USA) Cat# 05112-25; RRID: AB_3099697 Anti-Mouse CD28 SAFIRE Purified BioGem (USA) Cat# 10312-25; RRID: AB_3099698 Bacterial and virus strains Firefly Luciferase-mCherry Lentivirus GeneChem (China) N/A mCherry expressing lentivirus GeneChem (China) N/A Cell lines 4T1 ATCC N/A EMT6 ATCC N/A HUVEC ATCC N/A MDA-MB-231 ATCC N/A Chemicals, peptides, and recombinant proteins RPMI1640 HyClone (USA) N/A DMEM HyClone (USA) N/A Fetal bovine serum HyClone (USA) N/A ACK lysis buffer Haoyang (China) N/A PBS HyClone (USA) N/A 10xPBS Solarbio (China) Cat# P1022 Percoll HyClone (USA) Cat# 17-0819-09 Recombinant mouse IFNγ Prospec-Tany Cat# cyt-358 Recombinant human IFNγ Thermo (USA) Cat# 300-02-100UG RNA isolater Total RNA Extraction Reagent Vazyme (China) Cat# R401-01 HiScript III RT SuperMix for qPCR Vazyme (China) Cat# R323-01 Hieff qPCR SYBR Green Master Mix YEASEN (China) Cat# 11201ES03 Ionomycin YEASEN (China) Cat# 50402ES03 PMA Sigma-Aldrich Cat# P1585 Brefeldin A Beyotime (China) Cat# S1536 (Continued on next page) e1 Cell Reports Medicine 7, 102868, July 21, 2026 Article ll OPEN ACCESS .. The murine triple-negative breast cancer cells (4T1 and EMT6), human umbilical vein endothelial cells (HUVEC), and human triplenegative breast cancer cells (MDA-MB-231) were purchased from the American Type Culture Collection (ATCC).

Immunoprecipitation:

Article Title: LRP1 activated by AT2 cell-secreted MDK inhibits fibrotic ferroptosis in idiopathic pulmonary fibrosis
Article Snippet: .. Antibodies CTHRC1 Novus Cat#81744; RRID: AB_3414573 PDPN DSHB Cat# 8.1.1; RRID: AB_531893 SFTPC Merck Cat#ABC99; RRID: AB_2755001 ACSL4 CST Cat#9189 SLC7A11 CST Cat#12691; RRID: AB_2687474 GPX4 abcam Cat#ab125066; RRID: AB_10973901 FTH1 CST Cat#4393; RRID: AB_11217441 Beta-actin CST Cat#3700 LRP1 Invitrogen Cat#MA1-80192; RRID: AB_929727 MDK Invitrogen Cat#MA5-32538; RRID: AB_2809815 OTUB1 OriGene Cat#TA505150; RRID: AB_2622874 AKT Proteintech Cat#10176-2-AP; RRID: AB_2224574 p-AKT Proteintech Cat#66444-1-Ig; RRID: AB_2782958 p38 Proteintech Cat#14064-1-AP; RRID: AB_2878007 p-p38 Proteintech Cat#81212-2-RR; RRID: AB_3743373 Ubiquitin Invitrogen Cat#14-6078-82; RRID: AB_837154 Myc-Tag CST Cat#2276 FLAG Tag Sigma-Aldrich Cat#F3165; RRID: AB_259529 GST-Tag CST Cat#2624; RRID: AB_2189875 His-Tag CST Cat#2366; RRID: AB_2115719 HA-Tag CST Cat3724; RRID: AB_1549585 Bacterial and virus strains pAAV-SP-Cp-SV40 PolyA Genechem GCNV0402707 mCOL1A2-SV40 PolyA Genechem GCNV0402708 Chemicals, peptides, and recombinant proteins Bleomycin MedChemExpress HY-17565A PBS Gibico 20012027 4% paraformaldehyde Beyotime P0099 citrate buffer (pH 6.0) Thermo Fisher Scientific 005000 HRP-labelled goat anti-mouse/rabbit IgG abcam ab6721 DMEM Thermo Fisher Scientific 11965 FBS Thermo Fisher Scientific 10099141c Antibiotic-antimycotic Thermo Fisher Scientific 15240062 BODIPYTM 581/591 C11 fluorescence probe Thermo Fisher Scientific D3861 RIPA Lysis Buffer Beyotime P0013B Immunoprecipitation (IP) lysis buffer Thermo Fisher Scientific 87787 protein A/G-beads Thermo Fisher Scientific 88802 Critical commercial assays Hydroxyproline Assay Kit (Colorimetric) abcam ab222941 Human MDK ELISA Kit abcam ab193761 Mouse MDK ELISA Kit abcam ab279416 Masson’s trichrome staining Kit Solarbio G1340 (Continued on next page) iScience 29, 116212, June 19, 2026 e1 ..

Hydroxyproline Assay:

Article Title: LRP1 activated by AT2 cell-secreted MDK inhibits fibrotic ferroptosis in idiopathic pulmonary fibrosis
Article Snippet: .. Antibodies CTHRC1 Novus Cat#81744; RRID: AB_3414573 PDPN DSHB Cat# 8.1.1; RRID: AB_531893 SFTPC Merck Cat#ABC99; RRID: AB_2755001 ACSL4 CST Cat#9189 SLC7A11 CST Cat#12691; RRID: AB_2687474 GPX4 abcam Cat#ab125066; RRID: AB_10973901 FTH1 CST Cat#4393; RRID: AB_11217441 Beta-actin CST Cat#3700 LRP1 Invitrogen Cat#MA1-80192; RRID: AB_929727 MDK Invitrogen Cat#MA5-32538; RRID: AB_2809815 OTUB1 OriGene Cat#TA505150; RRID: AB_2622874 AKT Proteintech Cat#10176-2-AP; RRID: AB_2224574 p-AKT Proteintech Cat#66444-1-Ig; RRID: AB_2782958 p38 Proteintech Cat#14064-1-AP; RRID: AB_2878007 p-p38 Proteintech Cat#81212-2-RR; RRID: AB_3743373 Ubiquitin Invitrogen Cat#14-6078-82; RRID: AB_837154 Myc-Tag CST Cat#2276 FLAG Tag Sigma-Aldrich Cat#F3165; RRID: AB_259529 GST-Tag CST Cat#2624; RRID: AB_2189875 His-Tag CST Cat#2366; RRID: AB_2115719 HA-Tag CST Cat3724; RRID: AB_1549585 Bacterial and virus strains pAAV-SP-Cp-SV40 PolyA Genechem GCNV0402707 mCOL1A2-SV40 PolyA Genechem GCNV0402708 Chemicals, peptides, and recombinant proteins Bleomycin MedChemExpress HY-17565A PBS Gibico 20012027 4% paraformaldehyde Beyotime P0099 citrate buffer (pH 6.0) Thermo Fisher Scientific 005000 HRP-labelled goat anti-mouse/rabbit IgG abcam ab6721 DMEM Thermo Fisher Scientific 11965 FBS Thermo Fisher Scientific 10099141c Antibiotic-antimycotic Thermo Fisher Scientific 15240062 BODIPYTM 581/591 C11 fluorescence probe Thermo Fisher Scientific D3861 RIPA Lysis Buffer Beyotime P0013B Immunoprecipitation (IP) lysis buffer Thermo Fisher Scientific 87787 protein A/G-beads Thermo Fisher Scientific 88802 Critical commercial assays Hydroxyproline Assay Kit (Colorimetric) abcam ab222941 Human MDK ELISA Kit abcam ab193761 Mouse MDK ELISA Kit abcam ab279416 Masson’s trichrome staining Kit Solarbio G1340 (Continued on next page) iScience 29, 116212, June 19, 2026 e1 ..

Enzyme-linked Immunosorbent Assay:

Article Title: LRP1 activated by AT2 cell-secreted MDK inhibits fibrotic ferroptosis in idiopathic pulmonary fibrosis
Article Snippet: .. Antibodies CTHRC1 Novus Cat#81744; RRID: AB_3414573 PDPN DSHB Cat# 8.1.1; RRID: AB_531893 SFTPC Merck Cat#ABC99; RRID: AB_2755001 ACSL4 CST Cat#9189 SLC7A11 CST Cat#12691; RRID: AB_2687474 GPX4 abcam Cat#ab125066; RRID: AB_10973901 FTH1 CST Cat#4393; RRID: AB_11217441 Beta-actin CST Cat#3700 LRP1 Invitrogen Cat#MA1-80192; RRID: AB_929727 MDK Invitrogen Cat#MA5-32538; RRID: AB_2809815 OTUB1 OriGene Cat#TA505150; RRID: AB_2622874 AKT Proteintech Cat#10176-2-AP; RRID: AB_2224574 p-AKT Proteintech Cat#66444-1-Ig; RRID: AB_2782958 p38 Proteintech Cat#14064-1-AP; RRID: AB_2878007 p-p38 Proteintech Cat#81212-2-RR; RRID: AB_3743373 Ubiquitin Invitrogen Cat#14-6078-82; RRID: AB_837154 Myc-Tag CST Cat#2276 FLAG Tag Sigma-Aldrich Cat#F3165; RRID: AB_259529 GST-Tag CST Cat#2624; RRID: AB_2189875 His-Tag CST Cat#2366; RRID: AB_2115719 HA-Tag CST Cat3724; RRID: AB_1549585 Bacterial and virus strains pAAV-SP-Cp-SV40 PolyA Genechem GCNV0402707 mCOL1A2-SV40 PolyA Genechem GCNV0402708 Chemicals, peptides, and recombinant proteins Bleomycin MedChemExpress HY-17565A PBS Gibico 20012027 4% paraformaldehyde Beyotime P0099 citrate buffer (pH 6.0) Thermo Fisher Scientific 005000 HRP-labelled goat anti-mouse/rabbit IgG abcam ab6721 DMEM Thermo Fisher Scientific 11965 FBS Thermo Fisher Scientific 10099141c Antibiotic-antimycotic Thermo Fisher Scientific 15240062 BODIPYTM 581/591 C11 fluorescence probe Thermo Fisher Scientific D3861 RIPA Lysis Buffer Beyotime P0013B Immunoprecipitation (IP) lysis buffer Thermo Fisher Scientific 87787 protein A/G-beads Thermo Fisher Scientific 88802 Critical commercial assays Hydroxyproline Assay Kit (Colorimetric) abcam ab222941 Human MDK ELISA Kit abcam ab193761 Mouse MDK ELISA Kit abcam ab279416 Masson’s trichrome staining Kit Solarbio G1340 (Continued on next page) iScience 29, 116212, June 19, 2026 e1 ..

Staining:

Article Title: LRP1 activated by AT2 cell-secreted MDK inhibits fibrotic ferroptosis in idiopathic pulmonary fibrosis
Article Snippet: .. Antibodies CTHRC1 Novus Cat#81744; RRID: AB_3414573 PDPN DSHB Cat# 8.1.1; RRID: AB_531893 SFTPC Merck Cat#ABC99; RRID: AB_2755001 ACSL4 CST Cat#9189 SLC7A11 CST Cat#12691; RRID: AB_2687474 GPX4 abcam Cat#ab125066; RRID: AB_10973901 FTH1 CST Cat#4393; RRID: AB_11217441 Beta-actin CST Cat#3700 LRP1 Invitrogen Cat#MA1-80192; RRID: AB_929727 MDK Invitrogen Cat#MA5-32538; RRID: AB_2809815 OTUB1 OriGene Cat#TA505150; RRID: AB_2622874 AKT Proteintech Cat#10176-2-AP; RRID: AB_2224574 p-AKT Proteintech Cat#66444-1-Ig; RRID: AB_2782958 p38 Proteintech Cat#14064-1-AP; RRID: AB_2878007 p-p38 Proteintech Cat#81212-2-RR; RRID: AB_3743373 Ubiquitin Invitrogen Cat#14-6078-82; RRID: AB_837154 Myc-Tag CST Cat#2276 FLAG Tag Sigma-Aldrich Cat#F3165; RRID: AB_259529 GST-Tag CST Cat#2624; RRID: AB_2189875 His-Tag CST Cat#2366; RRID: AB_2115719 HA-Tag CST Cat3724; RRID: AB_1549585 Bacterial and virus strains pAAV-SP-Cp-SV40 PolyA Genechem GCNV0402707 mCOL1A2-SV40 PolyA Genechem GCNV0402708 Chemicals, peptides, and recombinant proteins Bleomycin MedChemExpress HY-17565A PBS Gibico 20012027 4% paraformaldehyde Beyotime P0099 citrate buffer (pH 6.0) Thermo Fisher Scientific 005000 HRP-labelled goat anti-mouse/rabbit IgG abcam ab6721 DMEM Thermo Fisher Scientific 11965 FBS Thermo Fisher Scientific 10099141c Antibiotic-antimycotic Thermo Fisher Scientific 15240062 BODIPYTM 581/591 C11 fluorescence probe Thermo Fisher Scientific D3861 RIPA Lysis Buffer Beyotime P0013B Immunoprecipitation (IP) lysis buffer Thermo Fisher Scientific 87787 protein A/G-beads Thermo Fisher Scientific 88802 Critical commercial assays Hydroxyproline Assay Kit (Colorimetric) abcam ab222941 Human MDK ELISA Kit abcam ab193761 Mouse MDK ELISA Kit abcam ab279416 Masson’s trichrome staining Kit Solarbio G1340 (Continued on next page) iScience 29, 116212, June 19, 2026 e1 ..

Lactate Dehydrogenase Assay:

Article Title: A preclinical candidate of cyclophilin D inhibition improves alcohol-associated liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mitofusin 1 antibody Abcam Cat#ab221661; RRID:AB_2941083 Anti-Mitofusin 2 antibody Abcam Cat#ab124773; RRID:AB_10999860 Anti-Cytochrome C antibody Abcam Cat#ab133504; RRID:AB_2802115 Anti-Nrf2 antibody Abcam Cat#ab92946; RRID:AB_10561604 Anti-SOD1 antibody Abcam Cat#ab16831; RRID:AB_302535 Anti-Catalase antibody Abcam Cat#ab52477; RRID:AB_868694 Anti-CYP2E1 antibody Abcam Cat#ab28146; RRID:AB_2089985 Anti-DRP1 antibody Cell Signaling Technology Cat#8570; RRID:AB_10950498 Anti-FIS1 antibody Cell Signaling Technology Cat#32525; RRID:AB_2927422 Anti-OPA1 antibody Cell Signaling Technology Cat#80471; RRID:AB_2734117 Anti-Bcl-2 antibody Cell Signaling Technology Cat#3498; RRID:AB_1903907 Anti-Bax antibody Cell Signaling Technology Cat#2772; RRID:AB_10695870 Anti-PCNA antibody Cell Signaling Technology Cat#13110; RRID:AB_2636979 Anti- iNOS antibody Cell Signaling Technology Cat#13120; RRID:AB_2687529 Anti-Cleaved Caspase-3 antibody Cell Signaling Technology Cat#9661; RRID:AB_2341188 Anti-NF-kappaB p65 antibody Cell Signaling Technology Cat#8242; RRID:AB_10859369 Anti-Phospho-NF-kappaB p65 antibody Cell Signaling Technology Cat#3033; RRID:AB_331284 Anti-GAPDH antibody Cell Signaling Technology Cat#2118; RRID:AB_561053 Anti-Phospho-Nrf2 antibody Affinity Biosciences CatDF7519 Bacterial and virus strains AAV8-ApoE/hAATp-EGFP-FT2ANM_134084-3Flag-SV40 PolyA GeneChem N/A AAV8-hAAT-shPpia-EGFP GeneChem N/A AAV8-hAAT-shPpib-EGFP GeneChem N/A Biological samples Healthy/ALD adult liver tissue This paper N/A Chemicals, peptides, and recombinant proteins RN-0001 Farsight Medical Technology N/A Debio-025 MedChemExpress Cat#HY-12559 CRV431 MedChemExpress Cat#HY-135644 Critical commercial assays Cytotoxicity LDH Assay Kit MedChemExpress Cat#HY-K1090 Apoptosis detection kit Vazyme Cat#A211-01 Calcineurin phosphatase assay kit Enzo Life Sciences Cat#BML-AK804-0001 Calcium colorimetric assay kit Beyotime Biotechnology Cat#S1063S Mitochondria isolation kit MedChemExpress Cat#HY-K1061 ATP/ADP ratio assay kit Abcam Cat#ab65313 Triglyceride content detection kit Solarbio Cat#BC0625 Non-esterified FFA assay kit Elabscience Cat#E-BC-K013 Deposited data RN-0001 This paper PubChem CID: 146639799 RNA-seq data of mouse liver This paper GEO: GSE316128 Single-cell RNA-seq data of human liver MacParland et al.54 GEO: GSE115469 (Continued on next page) e1 Cell Reports Medicine 7, 102654, March 17, 2026 ..

Phosphatase Assay:

Article Title: A preclinical candidate of cyclophilin D inhibition improves alcohol-associated liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mitofusin 1 antibody Abcam Cat#ab221661; RRID:AB_2941083 Anti-Mitofusin 2 antibody Abcam Cat#ab124773; RRID:AB_10999860 Anti-Cytochrome C antibody Abcam Cat#ab133504; RRID:AB_2802115 Anti-Nrf2 antibody Abcam Cat#ab92946; RRID:AB_10561604 Anti-SOD1 antibody Abcam Cat#ab16831; RRID:AB_302535 Anti-Catalase antibody Abcam Cat#ab52477; RRID:AB_868694 Anti-CYP2E1 antibody Abcam Cat#ab28146; RRID:AB_2089985 Anti-DRP1 antibody Cell Signaling Technology Cat#8570; RRID:AB_10950498 Anti-FIS1 antibody Cell Signaling Technology Cat#32525; RRID:AB_2927422 Anti-OPA1 antibody Cell Signaling Technology Cat#80471; RRID:AB_2734117 Anti-Bcl-2 antibody Cell Signaling Technology Cat#3498; RRID:AB_1903907 Anti-Bax antibody Cell Signaling Technology Cat#2772; RRID:AB_10695870 Anti-PCNA antibody Cell Signaling Technology Cat#13110; RRID:AB_2636979 Anti- iNOS antibody Cell Signaling Technology Cat#13120; RRID:AB_2687529 Anti-Cleaved Caspase-3 antibody Cell Signaling Technology Cat#9661; RRID:AB_2341188 Anti-NF-kappaB p65 antibody Cell Signaling Technology Cat#8242; RRID:AB_10859369 Anti-Phospho-NF-kappaB p65 antibody Cell Signaling Technology Cat#3033; RRID:AB_331284 Anti-GAPDH antibody Cell Signaling Technology Cat#2118; RRID:AB_561053 Anti-Phospho-Nrf2 antibody Affinity Biosciences CatDF7519 Bacterial and virus strains AAV8-ApoE/hAATp-EGFP-FT2ANM_134084-3Flag-SV40 PolyA GeneChem N/A AAV8-hAAT-shPpia-EGFP GeneChem N/A AAV8-hAAT-shPpib-EGFP GeneChem N/A Biological samples Healthy/ALD adult liver tissue This paper N/A Chemicals, peptides, and recombinant proteins RN-0001 Farsight Medical Technology N/A Debio-025 MedChemExpress Cat#HY-12559 CRV431 MedChemExpress Cat#HY-135644 Critical commercial assays Cytotoxicity LDH Assay Kit MedChemExpress Cat#HY-K1090 Apoptosis detection kit Vazyme Cat#A211-01 Calcineurin phosphatase assay kit Enzo Life Sciences Cat#BML-AK804-0001 Calcium colorimetric assay kit Beyotime Biotechnology Cat#S1063S Mitochondria isolation kit MedChemExpress Cat#HY-K1061 ATP/ADP ratio assay kit Abcam Cat#ab65313 Triglyceride content detection kit Solarbio Cat#BC0625 Non-esterified FFA assay kit Elabscience Cat#E-BC-K013 Deposited data RN-0001 This paper PubChem CID: 146639799 RNA-seq data of mouse liver This paper GEO: GSE316128 Single-cell RNA-seq data of human liver MacParland et al.54 GEO: GSE115469 (Continued on next page) e1 Cell Reports Medicine 7, 102654, March 17, 2026 ..

Calcium Colorimetric Assay:

Article Title: A preclinical candidate of cyclophilin D inhibition improves alcohol-associated liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mitofusin 1 antibody Abcam Cat#ab221661; RRID:AB_2941083 Anti-Mitofusin 2 antibody Abcam Cat#ab124773; RRID:AB_10999860 Anti-Cytochrome C antibody Abcam Cat#ab133504; RRID:AB_2802115 Anti-Nrf2 antibody Abcam Cat#ab92946; RRID:AB_10561604 Anti-SOD1 antibody Abcam Cat#ab16831; RRID:AB_302535 Anti-Catalase antibody Abcam Cat#ab52477; RRID:AB_868694 Anti-CYP2E1 antibody Abcam Cat#ab28146; RRID:AB_2089985 Anti-DRP1 antibody Cell Signaling Technology Cat#8570; RRID:AB_10950498 Anti-FIS1 antibody Cell Signaling Technology Cat#32525; RRID:AB_2927422 Anti-OPA1 antibody Cell Signaling Technology Cat#80471; RRID:AB_2734117 Anti-Bcl-2 antibody Cell Signaling Technology Cat#3498; RRID:AB_1903907 Anti-Bax antibody Cell Signaling Technology Cat#2772; RRID:AB_10695870 Anti-PCNA antibody Cell Signaling Technology Cat#13110; RRID:AB_2636979 Anti- iNOS antibody Cell Signaling Technology Cat#13120; RRID:AB_2687529 Anti-Cleaved Caspase-3 antibody Cell Signaling Technology Cat#9661; RRID:AB_2341188 Anti-NF-kappaB p65 antibody Cell Signaling Technology Cat#8242; RRID:AB_10859369 Anti-Phospho-NF-kappaB p65 antibody Cell Signaling Technology Cat#3033; RRID:AB_331284 Anti-GAPDH antibody Cell Signaling Technology Cat#2118; RRID:AB_561053 Anti-Phospho-Nrf2 antibody Affinity Biosciences CatDF7519 Bacterial and virus strains AAV8-ApoE/hAATp-EGFP-FT2ANM_134084-3Flag-SV40 PolyA GeneChem N/A AAV8-hAAT-shPpia-EGFP GeneChem N/A AAV8-hAAT-shPpib-EGFP GeneChem N/A Biological samples Healthy/ALD adult liver tissue This paper N/A Chemicals, peptides, and recombinant proteins RN-0001 Farsight Medical Technology N/A Debio-025 MedChemExpress Cat#HY-12559 CRV431 MedChemExpress Cat#HY-135644 Critical commercial assays Cytotoxicity LDH Assay Kit MedChemExpress Cat#HY-K1090 Apoptosis detection kit Vazyme Cat#A211-01 Calcineurin phosphatase assay kit Enzo Life Sciences Cat#BML-AK804-0001 Calcium colorimetric assay kit Beyotime Biotechnology Cat#S1063S Mitochondria isolation kit MedChemExpress Cat#HY-K1061 ATP/ADP ratio assay kit Abcam Cat#ab65313 Triglyceride content detection kit Solarbio Cat#BC0625 Non-esterified FFA assay kit Elabscience Cat#E-BC-K013 Deposited data RN-0001 This paper PubChem CID: 146639799 RNA-seq data of mouse liver This paper GEO: GSE316128 Single-cell RNA-seq data of human liver MacParland et al.54 GEO: GSE115469 (Continued on next page) e1 Cell Reports Medicine 7, 102654, March 17, 2026 ..

Isolation:

Article Title: A preclinical candidate of cyclophilin D inhibition improves alcohol-associated liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mitofusin 1 antibody Abcam Cat#ab221661; RRID:AB_2941083 Anti-Mitofusin 2 antibody Abcam Cat#ab124773; RRID:AB_10999860 Anti-Cytochrome C antibody Abcam Cat#ab133504; RRID:AB_2802115 Anti-Nrf2 antibody Abcam Cat#ab92946; RRID:AB_10561604 Anti-SOD1 antibody Abcam Cat#ab16831; RRID:AB_302535 Anti-Catalase antibody Abcam Cat#ab52477; RRID:AB_868694 Anti-CYP2E1 antibody Abcam Cat#ab28146; RRID:AB_2089985 Anti-DRP1 antibody Cell Signaling Technology Cat#8570; RRID:AB_10950498 Anti-FIS1 antibody Cell Signaling Technology Cat#32525; RRID:AB_2927422 Anti-OPA1 antibody Cell Signaling Technology Cat#80471; RRID:AB_2734117 Anti-Bcl-2 antibody Cell Signaling Technology Cat#3498; RRID:AB_1903907 Anti-Bax antibody Cell Signaling Technology Cat#2772; RRID:AB_10695870 Anti-PCNA antibody Cell Signaling Technology Cat#13110; RRID:AB_2636979 Anti- iNOS antibody Cell Signaling Technology Cat#13120; RRID:AB_2687529 Anti-Cleaved Caspase-3 antibody Cell Signaling Technology Cat#9661; RRID:AB_2341188 Anti-NF-kappaB p65 antibody Cell Signaling Technology Cat#8242; RRID:AB_10859369 Anti-Phospho-NF-kappaB p65 antibody Cell Signaling Technology Cat#3033; RRID:AB_331284 Anti-GAPDH antibody Cell Signaling Technology Cat#2118; RRID:AB_561053 Anti-Phospho-Nrf2 antibody Affinity Biosciences CatDF7519 Bacterial and virus strains AAV8-ApoE/hAATp-EGFP-FT2ANM_134084-3Flag-SV40 PolyA GeneChem N/A AAV8-hAAT-shPpia-EGFP GeneChem N/A AAV8-hAAT-shPpib-EGFP GeneChem N/A Biological samples Healthy/ALD adult liver tissue This paper N/A Chemicals, peptides, and recombinant proteins RN-0001 Farsight Medical Technology N/A Debio-025 MedChemExpress Cat#HY-12559 CRV431 MedChemExpress Cat#HY-135644 Critical commercial assays Cytotoxicity LDH Assay Kit MedChemExpress Cat#HY-K1090 Apoptosis detection kit Vazyme Cat#A211-01 Calcineurin phosphatase assay kit Enzo Life Sciences Cat#BML-AK804-0001 Calcium colorimetric assay kit Beyotime Biotechnology Cat#S1063S Mitochondria isolation kit MedChemExpress Cat#HY-K1061 ATP/ADP ratio assay kit Abcam Cat#ab65313 Triglyceride content detection kit Solarbio Cat#BC0625 Non-esterified FFA assay kit Elabscience Cat#E-BC-K013 Deposited data RN-0001 This paper PubChem CID: 146639799 RNA-seq data of mouse liver This paper GEO: GSE316128 Single-cell RNA-seq data of human liver MacParland et al.54 GEO: GSE115469 (Continued on next page) e1 Cell Reports Medicine 7, 102654, March 17, 2026 ..

RNA Sequencing:

Article Title: A preclinical candidate of cyclophilin D inhibition improves alcohol-associated liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mitofusin 1 antibody Abcam Cat#ab221661; RRID:AB_2941083 Anti-Mitofusin 2 antibody Abcam Cat#ab124773; RRID:AB_10999860 Anti-Cytochrome C antibody Abcam Cat#ab133504; RRID:AB_2802115 Anti-Nrf2 antibody Abcam Cat#ab92946; RRID:AB_10561604 Anti-SOD1 antibody Abcam Cat#ab16831; RRID:AB_302535 Anti-Catalase antibody Abcam Cat#ab52477; RRID:AB_868694 Anti-CYP2E1 antibody Abcam Cat#ab28146; RRID:AB_2089985 Anti-DRP1 antibody Cell Signaling Technology Cat#8570; RRID:AB_10950498 Anti-FIS1 antibody Cell Signaling Technology Cat#32525; RRID:AB_2927422 Anti-OPA1 antibody Cell Signaling Technology Cat#80471; RRID:AB_2734117 Anti-Bcl-2 antibody Cell Signaling Technology Cat#3498; RRID:AB_1903907 Anti-Bax antibody Cell Signaling Technology Cat#2772; RRID:AB_10695870 Anti-PCNA antibody Cell Signaling Technology Cat#13110; RRID:AB_2636979 Anti- iNOS antibody Cell Signaling Technology Cat#13120; RRID:AB_2687529 Anti-Cleaved Caspase-3 antibody Cell Signaling Technology Cat#9661; RRID:AB_2341188 Anti-NF-kappaB p65 antibody Cell Signaling Technology Cat#8242; RRID:AB_10859369 Anti-Phospho-NF-kappaB p65 antibody Cell Signaling Technology Cat#3033; RRID:AB_331284 Anti-GAPDH antibody Cell Signaling Technology Cat#2118; RRID:AB_561053 Anti-Phospho-Nrf2 antibody Affinity Biosciences CatDF7519 Bacterial and virus strains AAV8-ApoE/hAATp-EGFP-FT2ANM_134084-3Flag-SV40 PolyA GeneChem N/A AAV8-hAAT-shPpia-EGFP GeneChem N/A AAV8-hAAT-shPpib-EGFP GeneChem N/A Biological samples Healthy/ALD adult liver tissue This paper N/A Chemicals, peptides, and recombinant proteins RN-0001 Farsight Medical Technology N/A Debio-025 MedChemExpress Cat#HY-12559 CRV431 MedChemExpress Cat#HY-135644 Critical commercial assays Cytotoxicity LDH Assay Kit MedChemExpress Cat#HY-K1090 Apoptosis detection kit Vazyme Cat#A211-01 Calcineurin phosphatase assay kit Enzo Life Sciences Cat#BML-AK804-0001 Calcium colorimetric assay kit Beyotime Biotechnology Cat#S1063S Mitochondria isolation kit MedChemExpress Cat#HY-K1061 ATP/ADP ratio assay kit Abcam Cat#ab65313 Triglyceride content detection kit Solarbio Cat#BC0625 Non-esterified FFA assay kit Elabscience Cat#E-BC-K013 Deposited data RN-0001 This paper PubChem CID: 146639799 RNA-seq data of mouse liver This paper GEO: GSE316128 Single-cell RNA-seq data of human liver MacParland et al.54 GEO: GSE115469 (Continued on next page) e1 Cell Reports Medicine 7, 102654, March 17, 2026 ..

Single Cell:

Article Title: A preclinical candidate of cyclophilin D inhibition improves alcohol-associated liver injury.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-Mitofusin 1 antibody Abcam Cat#ab221661; RRID:AB_2941083 Anti-Mitofusin 2 antibody Abcam Cat#ab124773; RRID:AB_10999860 Anti-Cytochrome C antibody Abcam Cat#ab133504; RRID:AB_2802115 Anti-Nrf2 antibody Abcam Cat#ab92946; RRID:AB_10561604 Anti-SOD1 antibody Abcam Cat#ab16831; RRID:AB_302535 Anti-Catalase antibody Abcam Cat#ab52477; RRID:AB_868694 Anti-CYP2E1 antibody Abcam Cat#ab28146; RRID:AB_2089985 Anti-DRP1 antibody Cell Signaling Technology Cat#8570; RRID:AB_10950498 Anti-FIS1 antibody Cell Signaling Technology Cat#32525; RRID:AB_2927422 Anti-OPA1 antibody Cell Signaling Technology Cat#80471; RRID:AB_2734117 Anti-Bcl-2 antibody Cell Signaling Technology Cat#3498; RRID:AB_1903907 Anti-Bax antibody Cell Signaling Technology Cat#2772; RRID:AB_10695870 Anti-PCNA antibody Cell Signaling Technology Cat#13110; RRID:AB_2636979 Anti- iNOS antibody Cell Signaling Technology Cat#13120; RRID:AB_2687529 Anti-Cleaved Caspase-3 antibody Cell Signaling Technology Cat#9661; RRID:AB_2341188 Anti-NF-kappaB p65 antibody Cell Signaling Technology Cat#8242; RRID:AB_10859369 Anti-Phospho-NF-kappaB p65 antibody Cell Signaling Technology Cat#3033; RRID:AB_331284 Anti-GAPDH antibody Cell Signaling Technology Cat#2118; RRID:AB_561053 Anti-Phospho-Nrf2 antibody Affinity Biosciences CatDF7519 Bacterial and virus strains AAV8-ApoE/hAATp-EGFP-FT2ANM_134084-3Flag-SV40 PolyA GeneChem N/A AAV8-hAAT-shPpia-EGFP GeneChem N/A AAV8-hAAT-shPpib-EGFP GeneChem N/A Biological samples Healthy/ALD adult liver tissue This paper N/A Chemicals, peptides, and recombinant proteins RN-0001 Farsight Medical Technology N/A Debio-025 MedChemExpress Cat#HY-12559 CRV431 MedChemExpress Cat#HY-135644 Critical commercial assays Cytotoxicity LDH Assay Kit MedChemExpress Cat#HY-K1090 Apoptosis detection kit Vazyme Cat#A211-01 Calcineurin phosphatase assay kit Enzo Life Sciences Cat#BML-AK804-0001 Calcium colorimetric assay kit Beyotime Biotechnology Cat#S1063S Mitochondria isolation kit MedChemExpress Cat#HY-K1061 ATP/ADP ratio assay kit Abcam Cat#ab65313 Triglyceride content detection kit Solarbio Cat#BC0625 Non-esterified FFA assay kit Elabscience Cat#E-BC-K013 Deposited data RN-0001 This paper PubChem CID: 146639799 RNA-seq data of mouse liver This paper GEO: GSE316128 Single-cell RNA-seq data of human liver MacParland et al.54 GEO: GSE115469 (Continued on next page) e1 Cell Reports Medicine 7, 102654, March 17, 2026 ..

Purification:

Article Title: Non-genetically reprogrammed meta-neutrophils potentiate chemo-immunotherapy against lung metastatic triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse Ly-6G Antibody(Alexa Fluro 488) BioLegend (USA) Cat# 127626; RRID: AB_2561340 Anti-mouse/human CD11b Antibody (APC) BioLegend (USA) Cat# 101211; RRID: AB_312794 Anti-mouseTNF-α Antibody (PE) BioLegend (USA) Cat# 506306; RRID: AB_315427 Anti-mouse CD8a Antibody (APC/ Cyanine7) BioLegend (USA) Cat# 100714; RRID: AB_312753 Anti-mouseCD49b Antibody (Pacific BlueTM) BioLegend (USA) Cat# 108917; RRID: AB_2249376 Anti-mouse IFN-γ Antibody Antibody BioLegend (USA) Cat# 505810; RRID: AB_315404 Anti-human/mouse Granzyme B Antibody (FITC) BioLegend (USA) Cat# 515403; RRID: AB_2114575 Anti-mouse CD80 Antibody (FITC) BioLegend (USA) Cat# 104706; RRID: AB_313127 Anti-mouse CD86 Antibody ((Alexa Fluor 647) BioLegend (USA) Cat# 105019; RRID: AB_493465 Anti-mouse ICAM-1 Antibody (PE) Invitrogen (USA) Cat# 12-0541-81; RRID: AB_465706 Anti-mouse MHCII Antibody (PE) BioLegend (USA) Cat# 116407; RRID: AB_313726 Anti-mouse CD45 Antibody (PE) BioLegend (USA) Cat# 147711; RRID: AB_2563597 Anti-mouse F4/80 Antibody (APC) BioLegend (USA) Cat# 123115; RRID: AB_893493 Anti-mouse CD11c Antibody (FITC) BioLegend (USA) Cat# 117305; RRID: AB_313774 Anti-Mouse CD3 SAFIRE Purified BioGem (USA) Cat# 05112-25; RRID: AB_3099697 Anti-Mouse CD28 SAFIRE Purified BioGem (USA) Cat# 10312-25; RRID: AB_3099698 Bacterial and virus strains Firefly Luciferase-mCherry Lentivirus GeneChem (China) N/A mCherry expressing lentivirus GeneChem (China) N/A Cell lines 4T1 ATCC N/A EMT6 ATCC N/A HUVEC ATCC N/A MDA-MB-231 ATCC N/A Chemicals, peptides, and recombinant proteins RPMI1640 HyClone (USA) N/A DMEM HyClone (USA) N/A Fetal bovine serum HyClone (USA) N/A ACK lysis buffer Haoyang (China) N/A PBS HyClone (USA) N/A 10xPBS Solarbio (China) Cat# P1022 Percoll HyClone (USA) Cat# 17-0819-09 Recombinant mouse IFNγ Prospec-Tany Cat# cyt-358 Recombinant human IFNγ Thermo (USA) Cat# 300-02-100UG RNA isolater Total RNA Extraction Reagent Vazyme (China) Cat# R401-01 HiScript III RT SuperMix for qPCR Vazyme (China) Cat# R323-01 Hieff qPCR SYBR Green Master Mix YEASEN (China) Cat# 11201ES03 Ionomycin YEASEN (China) Cat# 50402ES03 PMA Sigma-Aldrich Cat# P1585 Brefeldin A Beyotime (China) Cat# S1536 (Continued on next page) e1 Cell Reports Medicine 7, 102868, July 21, 2026 Article ll OPEN ACCESS .. The murine triple-negative breast cancer cells (4T1 and EMT6), human umbilical vein endothelial cells (HUVEC), and human triplenegative breast cancer cells (MDA-MB-231) were purchased from the American Type Culture Collection (ATCC).

Luciferase:

Article Title: Non-genetically reprogrammed meta-neutrophils potentiate chemo-immunotherapy against lung metastatic triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse Ly-6G Antibody(Alexa Fluro 488) BioLegend (USA) Cat# 127626; RRID: AB_2561340 Anti-mouse/human CD11b Antibody (APC) BioLegend (USA) Cat# 101211; RRID: AB_312794 Anti-mouseTNF-α Antibody (PE) BioLegend (USA) Cat# 506306; RRID: AB_315427 Anti-mouse CD8a Antibody (APC/ Cyanine7) BioLegend (USA) Cat# 100714; RRID: AB_312753 Anti-mouseCD49b Antibody (Pacific BlueTM) BioLegend (USA) Cat# 108917; RRID: AB_2249376 Anti-mouse IFN-γ Antibody Antibody BioLegend (USA) Cat# 505810; RRID: AB_315404 Anti-human/mouse Granzyme B Antibody (FITC) BioLegend (USA) Cat# 515403; RRID: AB_2114575 Anti-mouse CD80 Antibody (FITC) BioLegend (USA) Cat# 104706; RRID: AB_313127 Anti-mouse CD86 Antibody ((Alexa Fluor 647) BioLegend (USA) Cat# 105019; RRID: AB_493465 Anti-mouse ICAM-1 Antibody (PE) Invitrogen (USA) Cat# 12-0541-81; RRID: AB_465706 Anti-mouse MHCII Antibody (PE) BioLegend (USA) Cat# 116407; RRID: AB_313726 Anti-mouse CD45 Antibody (PE) BioLegend (USA) Cat# 147711; RRID: AB_2563597 Anti-mouse F4/80 Antibody (APC) BioLegend (USA) Cat# 123115; RRID: AB_893493 Anti-mouse CD11c Antibody (FITC) BioLegend (USA) Cat# 117305; RRID: AB_313774 Anti-Mouse CD3 SAFIRE Purified BioGem (USA) Cat# 05112-25; RRID: AB_3099697 Anti-Mouse CD28 SAFIRE Purified BioGem (USA) Cat# 10312-25; RRID: AB_3099698 Bacterial and virus strains Firefly Luciferase-mCherry Lentivirus GeneChem (China) N/A mCherry expressing lentivirus GeneChem (China) N/A Cell lines 4T1 ATCC N/A EMT6 ATCC N/A HUVEC ATCC N/A MDA-MB-231 ATCC N/A Chemicals, peptides, and recombinant proteins RPMI1640 HyClone (USA) N/A DMEM HyClone (USA) N/A Fetal bovine serum HyClone (USA) N/A ACK lysis buffer Haoyang (China) N/A PBS HyClone (USA) N/A 10xPBS Solarbio (China) Cat# P1022 Percoll HyClone (USA) Cat# 17-0819-09 Recombinant mouse IFNγ Prospec-Tany Cat# cyt-358 Recombinant human IFNγ Thermo (USA) Cat# 300-02-100UG RNA isolater Total RNA Extraction Reagent Vazyme (China) Cat# R401-01 HiScript III RT SuperMix for qPCR Vazyme (China) Cat# R323-01 Hieff qPCR SYBR Green Master Mix YEASEN (China) Cat# 11201ES03 Ionomycin YEASEN (China) Cat# 50402ES03 PMA Sigma-Aldrich Cat# P1585 Brefeldin A Beyotime (China) Cat# S1536 (Continued on next page) e1 Cell Reports Medicine 7, 102868, July 21, 2026 Article ll OPEN ACCESS .. The murine triple-negative breast cancer cells (4T1 and EMT6), human umbilical vein endothelial cells (HUVEC), and human triplenegative breast cancer cells (MDA-MB-231) were purchased from the American Type Culture Collection (ATCC).

Expressing:

Article Title: Non-genetically reprogrammed meta-neutrophils potentiate chemo-immunotherapy against lung metastatic triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse Ly-6G Antibody(Alexa Fluro 488) BioLegend (USA) Cat# 127626; RRID: AB_2561340 Anti-mouse/human CD11b Antibody (APC) BioLegend (USA) Cat# 101211; RRID: AB_312794 Anti-mouseTNF-α Antibody (PE) BioLegend (USA) Cat# 506306; RRID: AB_315427 Anti-mouse CD8a Antibody (APC/ Cyanine7) BioLegend (USA) Cat# 100714; RRID: AB_312753 Anti-mouseCD49b Antibody (Pacific BlueTM) BioLegend (USA) Cat# 108917; RRID: AB_2249376 Anti-mouse IFN-γ Antibody Antibody BioLegend (USA) Cat# 505810; RRID: AB_315404 Anti-human/mouse Granzyme B Antibody (FITC) BioLegend (USA) Cat# 515403; RRID: AB_2114575 Anti-mouse CD80 Antibody (FITC) BioLegend (USA) Cat# 104706; RRID: AB_313127 Anti-mouse CD86 Antibody ((Alexa Fluor 647) BioLegend (USA) Cat# 105019; RRID: AB_493465 Anti-mouse ICAM-1 Antibody (PE) Invitrogen (USA) Cat# 12-0541-81; RRID: AB_465706 Anti-mouse MHCII Antibody (PE) BioLegend (USA) Cat# 116407; RRID: AB_313726 Anti-mouse CD45 Antibody (PE) BioLegend (USA) Cat# 147711; RRID: AB_2563597 Anti-mouse F4/80 Antibody (APC) BioLegend (USA) Cat# 123115; RRID: AB_893493 Anti-mouse CD11c Antibody (FITC) BioLegend (USA) Cat# 117305; RRID: AB_313774 Anti-Mouse CD3 SAFIRE Purified BioGem (USA) Cat# 05112-25; RRID: AB_3099697 Anti-Mouse CD28 SAFIRE Purified BioGem (USA) Cat# 10312-25; RRID: AB_3099698 Bacterial and virus strains Firefly Luciferase-mCherry Lentivirus GeneChem (China) N/A mCherry expressing lentivirus GeneChem (China) N/A Cell lines 4T1 ATCC N/A EMT6 ATCC N/A HUVEC ATCC N/A MDA-MB-231 ATCC N/A Chemicals, peptides, and recombinant proteins RPMI1640 HyClone (USA) N/A DMEM HyClone (USA) N/A Fetal bovine serum HyClone (USA) N/A ACK lysis buffer Haoyang (China) N/A PBS HyClone (USA) N/A 10xPBS Solarbio (China) Cat# P1022 Percoll HyClone (USA) Cat# 17-0819-09 Recombinant mouse IFNγ Prospec-Tany Cat# cyt-358 Recombinant human IFNγ Thermo (USA) Cat# 300-02-100UG RNA isolater Total RNA Extraction Reagent Vazyme (China) Cat# R401-01 HiScript III RT SuperMix for qPCR Vazyme (China) Cat# R323-01 Hieff qPCR SYBR Green Master Mix YEASEN (China) Cat# 11201ES03 Ionomycin YEASEN (China) Cat# 50402ES03 PMA Sigma-Aldrich Cat# P1585 Brefeldin A Beyotime (China) Cat# S1536 (Continued on next page) e1 Cell Reports Medicine 7, 102868, July 21, 2026 Article ll OPEN ACCESS .. The murine triple-negative breast cancer cells (4T1 and EMT6), human umbilical vein endothelial cells (HUVEC), and human triplenegative breast cancer cells (MDA-MB-231) were purchased from the American Type Culture Collection (ATCC).

Multiple Displacement Amplification:

Article Title: Non-genetically reprogrammed meta-neutrophils potentiate chemo-immunotherapy against lung metastatic triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse Ly-6G Antibody(Alexa Fluro 488) BioLegend (USA) Cat# 127626; RRID: AB_2561340 Anti-mouse/human CD11b Antibody (APC) BioLegend (USA) Cat# 101211; RRID: AB_312794 Anti-mouseTNF-α Antibody (PE) BioLegend (USA) Cat# 506306; RRID: AB_315427 Anti-mouse CD8a Antibody (APC/ Cyanine7) BioLegend (USA) Cat# 100714; RRID: AB_312753 Anti-mouseCD49b Antibody (Pacific BlueTM) BioLegend (USA) Cat# 108917; RRID: AB_2249376 Anti-mouse IFN-γ Antibody Antibody BioLegend (USA) Cat# 505810; RRID: AB_315404 Anti-human/mouse Granzyme B Antibody (FITC) BioLegend (USA) Cat# 515403; RRID: AB_2114575 Anti-mouse CD80 Antibody (FITC) BioLegend (USA) Cat# 104706; RRID: AB_313127 Anti-mouse CD86 Antibody ((Alexa Fluor 647) BioLegend (USA) Cat# 105019; RRID: AB_493465 Anti-mouse ICAM-1 Antibody (PE) Invitrogen (USA) Cat# 12-0541-81; RRID: AB_465706 Anti-mouse MHCII Antibody (PE) BioLegend (USA) Cat# 116407; RRID: AB_313726 Anti-mouse CD45 Antibody (PE) BioLegend (USA) Cat# 147711; RRID: AB_2563597 Anti-mouse F4/80 Antibody (APC) BioLegend (USA) Cat# 123115; RRID: AB_893493 Anti-mouse CD11c Antibody (FITC) BioLegend (USA) Cat# 117305; RRID: AB_313774 Anti-Mouse CD3 SAFIRE Purified BioGem (USA) Cat# 05112-25; RRID: AB_3099697 Anti-Mouse CD28 SAFIRE Purified BioGem (USA) Cat# 10312-25; RRID: AB_3099698 Bacterial and virus strains Firefly Luciferase-mCherry Lentivirus GeneChem (China) N/A mCherry expressing lentivirus GeneChem (China) N/A Cell lines 4T1 ATCC N/A EMT6 ATCC N/A HUVEC ATCC N/A MDA-MB-231 ATCC N/A Chemicals, peptides, and recombinant proteins RPMI1640 HyClone (USA) N/A DMEM HyClone (USA) N/A Fetal bovine serum HyClone (USA) N/A ACK lysis buffer Haoyang (China) N/A PBS HyClone (USA) N/A 10xPBS Solarbio (China) Cat# P1022 Percoll HyClone (USA) Cat# 17-0819-09 Recombinant mouse IFNγ Prospec-Tany Cat# cyt-358 Recombinant human IFNγ Thermo (USA) Cat# 300-02-100UG RNA isolater Total RNA Extraction Reagent Vazyme (China) Cat# R401-01 HiScript III RT SuperMix for qPCR Vazyme (China) Cat# R323-01 Hieff qPCR SYBR Green Master Mix YEASEN (China) Cat# 11201ES03 Ionomycin YEASEN (China) Cat# 50402ES03 PMA Sigma-Aldrich Cat# P1585 Brefeldin A Beyotime (China) Cat# S1536 (Continued on next page) e1 Cell Reports Medicine 7, 102868, July 21, 2026 Article ll OPEN ACCESS .. The murine triple-negative breast cancer cells (4T1 and EMT6), human umbilical vein endothelial cells (HUVEC), and human triplenegative breast cancer cells (MDA-MB-231) were purchased from the American Type Culture Collection (ATCC).

RNA Extraction:

Article Title: Non-genetically reprogrammed meta-neutrophils potentiate chemo-immunotherapy against lung metastatic triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse Ly-6G Antibody(Alexa Fluro 488) BioLegend (USA) Cat# 127626; RRID: AB_2561340 Anti-mouse/human CD11b Antibody (APC) BioLegend (USA) Cat# 101211; RRID: AB_312794 Anti-mouseTNF-α Antibody (PE) BioLegend (USA) Cat# 506306; RRID: AB_315427 Anti-mouse CD8a Antibody (APC/ Cyanine7) BioLegend (USA) Cat# 100714; RRID: AB_312753 Anti-mouseCD49b Antibody (Pacific BlueTM) BioLegend (USA) Cat# 108917; RRID: AB_2249376 Anti-mouse IFN-γ Antibody Antibody BioLegend (USA) Cat# 505810; RRID: AB_315404 Anti-human/mouse Granzyme B Antibody (FITC) BioLegend (USA) Cat# 515403; RRID: AB_2114575 Anti-mouse CD80 Antibody (FITC) BioLegend (USA) Cat# 104706; RRID: AB_313127 Anti-mouse CD86 Antibody ((Alexa Fluor 647) BioLegend (USA) Cat# 105019; RRID: AB_493465 Anti-mouse ICAM-1 Antibody (PE) Invitrogen (USA) Cat# 12-0541-81; RRID: AB_465706 Anti-mouse MHCII Antibody (PE) BioLegend (USA) Cat# 116407; RRID: AB_313726 Anti-mouse CD45 Antibody (PE) BioLegend (USA) Cat# 147711; RRID: AB_2563597 Anti-mouse F4/80 Antibody (APC) BioLegend (USA) Cat# 123115; RRID: AB_893493 Anti-mouse CD11c Antibody (FITC) BioLegend (USA) Cat# 117305; RRID: AB_313774 Anti-Mouse CD3 SAFIRE Purified BioGem (USA) Cat# 05112-25; RRID: AB_3099697 Anti-Mouse CD28 SAFIRE Purified BioGem (USA) Cat# 10312-25; RRID: AB_3099698 Bacterial and virus strains Firefly Luciferase-mCherry Lentivirus GeneChem (China) N/A mCherry expressing lentivirus GeneChem (China) N/A Cell lines 4T1 ATCC N/A EMT6 ATCC N/A HUVEC ATCC N/A MDA-MB-231 ATCC N/A Chemicals, peptides, and recombinant proteins RPMI1640 HyClone (USA) N/A DMEM HyClone (USA) N/A Fetal bovine serum HyClone (USA) N/A ACK lysis buffer Haoyang (China) N/A PBS HyClone (USA) N/A 10xPBS Solarbio (China) Cat# P1022 Percoll HyClone (USA) Cat# 17-0819-09 Recombinant mouse IFNγ Prospec-Tany Cat# cyt-358 Recombinant human IFNγ Thermo (USA) Cat# 300-02-100UG RNA isolater Total RNA Extraction Reagent Vazyme (China) Cat# R401-01 HiScript III RT SuperMix for qPCR Vazyme (China) Cat# R323-01 Hieff qPCR SYBR Green Master Mix YEASEN (China) Cat# 11201ES03 Ionomycin YEASEN (China) Cat# 50402ES03 PMA Sigma-Aldrich Cat# P1585 Brefeldin A Beyotime (China) Cat# S1536 (Continued on next page) e1 Cell Reports Medicine 7, 102868, July 21, 2026 Article ll OPEN ACCESS .. The murine triple-negative breast cancer cells (4T1 and EMT6), human umbilical vein endothelial cells (HUVEC), and human triplenegative breast cancer cells (MDA-MB-231) were purchased from the American Type Culture Collection (ATCC).

Real-time Polymerase Chain Reaction:

Article Title: Non-genetically reprogrammed meta-neutrophils potentiate chemo-immunotherapy against lung metastatic triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse Ly-6G Antibody(Alexa Fluro 488) BioLegend (USA) Cat# 127626; RRID: AB_2561340 Anti-mouse/human CD11b Antibody (APC) BioLegend (USA) Cat# 101211; RRID: AB_312794 Anti-mouseTNF-α Antibody (PE) BioLegend (USA) Cat# 506306; RRID: AB_315427 Anti-mouse CD8a Antibody (APC/ Cyanine7) BioLegend (USA) Cat# 100714; RRID: AB_312753 Anti-mouseCD49b Antibody (Pacific BlueTM) BioLegend (USA) Cat# 108917; RRID: AB_2249376 Anti-mouse IFN-γ Antibody Antibody BioLegend (USA) Cat# 505810; RRID: AB_315404 Anti-human/mouse Granzyme B Antibody (FITC) BioLegend (USA) Cat# 515403; RRID: AB_2114575 Anti-mouse CD80 Antibody (FITC) BioLegend (USA) Cat# 104706; RRID: AB_313127 Anti-mouse CD86 Antibody ((Alexa Fluor 647) BioLegend (USA) Cat# 105019; RRID: AB_493465 Anti-mouse ICAM-1 Antibody (PE) Invitrogen (USA) Cat# 12-0541-81; RRID: AB_465706 Anti-mouse MHCII Antibody (PE) BioLegend (USA) Cat# 116407; RRID: AB_313726 Anti-mouse CD45 Antibody (PE) BioLegend (USA) Cat# 147711; RRID: AB_2563597 Anti-mouse F4/80 Antibody (APC) BioLegend (USA) Cat# 123115; RRID: AB_893493 Anti-mouse CD11c Antibody (FITC) BioLegend (USA) Cat# 117305; RRID: AB_313774 Anti-Mouse CD3 SAFIRE Purified BioGem (USA) Cat# 05112-25; RRID: AB_3099697 Anti-Mouse CD28 SAFIRE Purified BioGem (USA) Cat# 10312-25; RRID: AB_3099698 Bacterial and virus strains Firefly Luciferase-mCherry Lentivirus GeneChem (China) N/A mCherry expressing lentivirus GeneChem (China) N/A Cell lines 4T1 ATCC N/A EMT6 ATCC N/A HUVEC ATCC N/A MDA-MB-231 ATCC N/A Chemicals, peptides, and recombinant proteins RPMI1640 HyClone (USA) N/A DMEM HyClone (USA) N/A Fetal bovine serum HyClone (USA) N/A ACK lysis buffer Haoyang (China) N/A PBS HyClone (USA) N/A 10xPBS Solarbio (China) Cat# P1022 Percoll HyClone (USA) Cat# 17-0819-09 Recombinant mouse IFNγ Prospec-Tany Cat# cyt-358 Recombinant human IFNγ Thermo (USA) Cat# 300-02-100UG RNA isolater Total RNA Extraction Reagent Vazyme (China) Cat# R401-01 HiScript III RT SuperMix for qPCR Vazyme (China) Cat# R323-01 Hieff qPCR SYBR Green Master Mix YEASEN (China) Cat# 11201ES03 Ionomycin YEASEN (China) Cat# 50402ES03 PMA Sigma-Aldrich Cat# P1585 Brefeldin A Beyotime (China) Cat# S1536 (Continued on next page) e1 Cell Reports Medicine 7, 102868, July 21, 2026 Article ll OPEN ACCESS .. The murine triple-negative breast cancer cells (4T1 and EMT6), human umbilical vein endothelial cells (HUVEC), and human triplenegative breast cancer cells (MDA-MB-231) were purchased from the American Type Culture Collection (ATCC).

SYBR Green Assay:

Article Title: Non-genetically reprogrammed meta-neutrophils potentiate chemo-immunotherapy against lung metastatic triple-negative breast cancer.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-mouse Ly-6G Antibody(Alexa Fluro 488) BioLegend (USA) Cat# 127626; RRID: AB_2561340 Anti-mouse/human CD11b Antibody (APC) BioLegend (USA) Cat# 101211; RRID: AB_312794 Anti-mouseTNF-α Antibody (PE) BioLegend (USA) Cat# 506306; RRID: AB_315427 Anti-mouse CD8a Antibody (APC/ Cyanine7) BioLegend (USA) Cat# 100714; RRID: AB_312753 Anti-mouseCD49b Antibody (Pacific BlueTM) BioLegend (USA) Cat# 108917; RRID: AB_2249376 Anti-mouse IFN-γ Antibody Antibody BioLegend (USA) Cat# 505810; RRID: AB_315404 Anti-human/mouse Granzyme B Antibody (FITC) BioLegend (USA) Cat# 515403; RRID: AB_2114575 Anti-mouse CD80 Antibody (FITC) BioLegend (USA) Cat# 104706; RRID: AB_313127 Anti-mouse CD86 Antibody ((Alexa Fluor 647) BioLegend (USA) Cat# 105019; RRID: AB_493465 Anti-mouse ICAM-1 Antibody (PE) Invitrogen (USA) Cat# 12-0541-81; RRID: AB_465706 Anti-mouse MHCII Antibody (PE) BioLegend (USA) Cat# 116407; RRID: AB_313726 Anti-mouse CD45 Antibody (PE) BioLegend (USA) Cat# 147711; RRID: AB_2563597 Anti-mouse F4/80 Antibody (APC) BioLegend (USA) Cat# 123115; RRID: AB_893493 Anti-mouse CD11c Antibody (FITC) BioLegend (USA) Cat# 117305; RRID: AB_313774 Anti-Mouse CD3 SAFIRE Purified BioGem (USA) Cat# 05112-25; RRID: AB_3099697 Anti-Mouse CD28 SAFIRE Purified BioGem (USA) Cat# 10312-25; RRID: AB_3099698 Bacterial and virus strains Firefly Luciferase-mCherry Lentivirus GeneChem (China) N/A mCherry expressing lentivirus GeneChem (China) N/A Cell lines 4T1 ATCC N/A EMT6 ATCC N/A HUVEC ATCC N/A MDA-MB-231 ATCC N/A Chemicals, peptides, and recombinant proteins RPMI1640 HyClone (USA) N/A DMEM HyClone (USA) N/A Fetal bovine serum HyClone (USA) N/A ACK lysis buffer Haoyang (China) N/A PBS HyClone (USA) N/A 10xPBS Solarbio (China) Cat# P1022 Percoll HyClone (USA) Cat# 17-0819-09 Recombinant mouse IFNγ Prospec-Tany Cat# cyt-358 Recombinant human IFNγ Thermo (USA) Cat# 300-02-100UG RNA isolater Total RNA Extraction Reagent Vazyme (China) Cat# R401-01 HiScript III RT SuperMix for qPCR Vazyme (China) Cat# R323-01 Hieff qPCR SYBR Green Master Mix YEASEN (China) Cat# 11201ES03 Ionomycin YEASEN (China) Cat# 50402ES03 PMA Sigma-Aldrich Cat# P1585 Brefeldin A Beyotime (China) Cat# S1536 (Continued on next page) e1 Cell Reports Medicine 7, 102868, July 21, 2026 Article ll OPEN ACCESS .. The murine triple-negative breast cancer cells (4T1 and EMT6), human umbilical vein endothelial cells (HUVEC), and human triplenegative breast cancer cells (MDA-MB-231) were purchased from the American Type Culture Collection (ATCC).

Over Expression:

Article Title: GALNT1 drives aggressive phenotypes of rheumatoid synoviocytes via NEK9 O‑glycosylation.
Article Snippet: .. GALNT1 overexpression (GALNT1-OE) lentivirus, 579 overexpression of NEK9 wild-type (NEK9-OE), and glycosylation-site mutant 580 (NEK9-mut), and vector control lentivirus were purchased from Genechem 581 (Guangzhou, China) and applied to FLSs at an MOI of 50 in the presence of 582 polybrene (10 μg/ml, H8761, Solarbio, China). ..

Glycoproteomics:

Article Title: GALNT1 drives aggressive phenotypes of rheumatoid synoviocytes via NEK9 O‑glycosylation.
Article Snippet: .. GALNT1 overexpression (GALNT1-OE) lentivirus, 579 overexpression of NEK9 wild-type (NEK9-OE), and glycosylation-site mutant 580 (NEK9-mut), and vector control lentivirus were purchased from Genechem 581 (Guangzhou, China) and applied to FLSs at an MOI of 50 in the presence of 582 polybrene (10 μg/ml, H8761, Solarbio, China). ..

Mutagenesis:

Article Title: GALNT1 drives aggressive phenotypes of rheumatoid synoviocytes via NEK9 O‑glycosylation.
Article Snippet: .. GALNT1 overexpression (GALNT1-OE) lentivirus, 579 overexpression of NEK9 wild-type (NEK9-OE), and glycosylation-site mutant 580 (NEK9-mut), and vector control lentivirus were purchased from Genechem 581 (Guangzhou, China) and applied to FLSs at an MOI of 50 in the presence of 582 polybrene (10 μg/ml, H8761, Solarbio, China). ..

Control:

Article Title: GALNT1 drives aggressive phenotypes of rheumatoid synoviocytes via NEK9 O‑glycosylation.
Article Snippet: .. GALNT1 overexpression (GALNT1-OE) lentivirus, 579 overexpression of NEK9 wild-type (NEK9-OE), and glycosylation-site mutant 580 (NEK9-mut), and vector control lentivirus were purchased from Genechem 581 (Guangzhou, China) and applied to FLSs at an MOI of 50 in the presence of 582 polybrene (10 μg/ml, H8761, Solarbio, China). ..



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