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polya polymerase buffer  (New England Biolabs)


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    New England Biolabs polya polymerase buffer
    Polya Polymerase Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polya+buffer/pm39198865-219-15-18
    Average 86 stars, based on 1 article reviews
    polya polymerase buffer - by Bioz Stars, 2026-09
    86/100 stars

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    Plot showing the sequencing coverage (green), location of the 3’ ends of reads (orange) and splicing patterns (purple lines connecting splice donors and acceptors) for two Wiskott-Aldrich Syndrome vectors sequenced by Nanopore direct RNA technology (A) WAS LV5 sequencing data (contains a single A to T mutation in the potential cryptic polyA site). (B) Reference sequence of the Wiskott-Aldrich Syndrome vector, with various features annotated.

    Journal: bioRxiv

    Article Title: An optimised protocol for quality control of gene therapy vectors using Nanopore direct RNA sequencing

    doi: 10.1101/2023.12.03.569756

    Figure Lengend Snippet: Plot showing the sequencing coverage (green), location of the 3’ ends of reads (orange) and splicing patterns (purple lines connecting splice donors and acceptors) for two Wiskott-Aldrich Syndrome vectors sequenced by Nanopore direct RNA technology (A) WAS LV5 sequencing data (contains a single A to T mutation in the potential cryptic polyA site). (B) Reference sequence of the Wiskott-Aldrich Syndrome vector, with various features annotated.

    Article Snippet: Artificial polyadenylation was carried out based on Crabtree et al. ( ) Reactions containing 15 μL of RNA (500 ng), 2 μL of polyA buffer, 2 μL of 10 mM ATP (NEB P0756S), 1 μL of E. coli Poly(A) Polymerase (NEB M0276S) and 0.5 μL of Murine RNase Inhibitor (NEB M0314S) were incubated for 30 minutes at 37°C, 20 minutes at 65°C and then 5 minutes at 98°C before being placed on ice.

    Techniques: Sequencing, Mutagenesis, Plasmid Preparation