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fastpure plasmid mini kit  (Vazyme Biotech Co)


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    Structured Review

    Vazyme Biotech Co fastpure plasmid mini kit
    Fastpure Plasmid Mini Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 96/100, based on 347 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plasmid+mini+kit/FastPure+Plasmid+Mini+Kit/pm42904557-122-6-10
    Average 96 stars, based on 347 article reviews
    fastpure plasmid mini kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Bacteria:

    Article Title: Bioinformatics Characterization of GRX3 Protein: Identification of Interacting Proteins and Molecular Docking Analysis with Patulin
    Article Snippet: GRX3 from Meyerozyma guilliermondii was significantly upregulated under patulin (PAT) stress, suggesting its potential involvement in mycotoxin detoxification.. In this study, comprehensive bioinformatics analyses were performed to characterize the GRX3 protein, followed by experimental verification of its subcellular localization and protein interactions.. The GRX3 protein consists of 231 amino acids with a molecular weight of 25.54 kDa and a theoretical pI of 4.72.

    Cell Culture:

    Article Title: Bioinformatics Characterization of GRX3 Protein: Identification of Interacting Proteins and Molecular Docking Analysis with Patulin
    Article Snippet: GRX3 from Meyerozyma guilliermondii was significantly upregulated under patulin (PAT) stress, suggesting its potential involvement in mycotoxin detoxification.. In this study, comprehensive bioinformatics analyses were performed to characterize the GRX3 protein, followed by experimental verification of its subcellular localization and protein interactions.. The GRX3 protein consists of 231 amino acids with a molecular weight of 25.54 kDa and a theoretical pI of 4.72.

    Incubation:

    Article Title: Bioinformatics Characterization of GRX3 Protein: Identification of Interacting Proteins and Molecular Docking Analysis with Patulin
    Article Snippet: GRX3 from Meyerozyma guilliermondii was significantly upregulated under patulin (PAT) stress, suggesting its potential involvement in mycotoxin detoxification.. In this study, comprehensive bioinformatics analyses were performed to characterize the GRX3 protein, followed by experimental verification of its subcellular localization and protein interactions.. The GRX3 protein consists of 231 amino acids with a molecular weight of 25.54 kDa and a theoretical pI of 4.72.

    Plasmid Preparation:

    Article Title: Bioinformatics Characterization of GRX3 Protein: Identification of Interacting Proteins and Molecular Docking Analysis with Patulin
    Article Snippet: GRX3 from Meyerozyma guilliermondii was significantly upregulated under patulin (PAT) stress, suggesting its potential involvement in mycotoxin detoxification.. In this study, comprehensive bioinformatics analyses were performed to characterize the GRX3 protein, followed by experimental verification of its subcellular localization and protein interactions.. The GRX3 protein consists of 231 amino acids with a molecular weight of 25.54 kDa and a theoretical pI of 4.72.

    Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Micrococcal Nuclease New England Biolabs Cat# M0247S ATP Sigma-Aldrich Cat# 11140965001 Glutaraldehyde solution Sigma-Aldrich Cat# G7651 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat# DLM-4-PK NADH Sigma-Aldrich Cat# 10128023001 Phosphoenol-pyruvate Roche Cat# 10108294001 Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle Sigma-Aldrich Cat# P0294 SYBR Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) Thermo Fisher Scientific Cat# S11494 LabBlue Coomassie Brilliant Blue Rapid Staining Solution LABLEAD Cat# G1042 Cellfectin II Reagent Thermo Fisher Scientific Cat# 10362100 Sf-900 III SFM Thermo Fisher Scientific Cat# 12658019 DMEM, high glucose, pyruvate Gibco, Thermo Fisher Scientific Cat# 11995073 Fetal Bovine Serum, Value Gibco, Thermo Fisher Scientific Cat# A5256701 Penicillin-Streptomycin (10,000 U/mL) Gibco, Thermo Fisher Scientific Cat# 15140122 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–12.e1–e7, December 3, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin-EDTA (0.25%), phenol red Gibco, Thermo Fisher Scientific Cat# 25200072 PBS (10X), pH 7.4 Gibco, Thermo Fisher Scientific Cat# 70011044 UltraPure DNase/RNase-Free Distilled Water Invitrogen, Thermo Fisher Scientific Cat# 10977015 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Fisher Scientific Cat# 78440 Pierce BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23225 Immobilon -P PVDF Membrane Millipore Cat# IPVH00010 WesternBright ECL HRP substrate Advansta Cat# K-12045-D20 Cell Counting Kit-8 (CCK-8) MCE Cat# HY-K0301 Pyridostatin trihydrochloride (PDS) MCE Cat# HY-15176C Deposited data ATRX in complex with the nucleosome (consensus map) This study EMDB: EMD-62684 ATRX with the nucleosomal DNA (focus refinement map) This study EMDB: EMD-62686 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state (composite map) This study EMDB: EMD-62690 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state This study PDB: 9L06 Experimental models: Cell lines Sf9 cells in Sf-900 II SFM Thermo Fisher Scientific Cat#11496015

    Article Title: Metabolic engineering of Saccharomyces cerevisiae for enhanced pregnenolone production through engineering the P450scc catalytic microenvironment
    Article Snippet: 1 School of Life Sciences and Health Engineering, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, China 2 College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China Abstract Pregnenolone is an important steroid precursor with broad pharmaceutical applications.. However, its traditional production mainly relies on semi-synthesis from plant-derived diosgenin, which is associated with resource limitations and high production costs.. In this study, a pregnenolone-producing Saccharomyces cerevisiae strain was systematically engineered by optimizing intracellular iron utilization, heme biosynthesis, and NADPH regeneration to improve the intracellular environment supporting the plant-derived P450scc system (CYP87A4).

    Article Title: Heatwave-Resilient Cuticular Microbiota Protect the Leaf Beetle Plagiodera versicolora From Fungal Infection.
    Article Snippet: .. Plasmid DNA was extracted using the FastPure Plasmid Mini Kit (Vazyme Biotech Co., Ltd., China), and its concentration was measured with a Nanodrop ND ‐2000 spectrophotometer (Nanodrop, USA). ..

    Article Title: Generation and Preliminary Characterization of a DsRed-MBD Microtubule Reporter Line in Ipomoea cordatotriloba
    Article Snippet: The pCAMBIA1300 vector was linearized by double digestion with Xba I and Sac I (New England Biolabs, R3142S, Ipswich, MA, USA) at 37 ◦C. .. The target linear vector band was recovered and gel-purified using a Gel Extraction Kit (Vazyme, DC201-01, Nanjing, China) after size verification by agarose gel electrophoresis. ..

    Article Title: Altered AMH signaling in Sertoli cells is associated with short photoperiod-induced testicular regression in Siberian hamsters.
    Article Snippet: Seasonal testicular regression is a key adaptive physiological process in seasonal breeding mammals, yet the changes in anti-Müllerian hormone (AMH) signaling associated with established testicular regression remain insufficiently understood.. In this study, we compared sexually mature Siberian hamsters maintained under long-, intermediate-, and short-photoperiod conditions for 8 weeks to characterize AMH-related changes after short photoperiod-induced testicular regression had been established.. Testicular morphology, AMH/AMHR2-SMAD signaling, and apoptosis-related parameters were examined.

    Isolation:

    Article Title: Bioinformatics Characterization of GRX3 Protein: Identification of Interacting Proteins and Molecular Docking Analysis with Patulin
    Article Snippet: GRX3 from Meyerozyma guilliermondii was significantly upregulated under patulin (PAT) stress, suggesting its potential involvement in mycotoxin detoxification.. In this study, comprehensive bioinformatics analyses were performed to characterize the GRX3 protein, followed by experimental verification of its subcellular localization and protein interactions.. The GRX3 protein consists of 231 amino acids with a molecular weight of 25.54 kDa and a theoretical pI of 4.72.

    Lysis:

    Article Title: Bioinformatics Characterization of GRX3 Protein: Identification of Interacting Proteins and Molecular Docking Analysis with Patulin
    Article Snippet: GRX3 from Meyerozyma guilliermondii was significantly upregulated under patulin (PAT) stress, suggesting its potential involvement in mycotoxin detoxification.. In this study, comprehensive bioinformatics analyses were performed to characterize the GRX3 protein, followed by experimental verification of its subcellular localization and protein interactions.. The GRX3 protein consists of 231 amino acids with a molecular weight of 25.54 kDa and a theoretical pI of 4.72.

    Neutralization:

    Article Title: Bioinformatics Characterization of GRX3 Protein: Identification of Interacting Proteins and Molecular Docking Analysis with Patulin
    Article Snippet: GRX3 from Meyerozyma guilliermondii was significantly upregulated under patulin (PAT) stress, suggesting its potential involvement in mycotoxin detoxification.. In this study, comprehensive bioinformatics analyses were performed to characterize the GRX3 protein, followed by experimental verification of its subcellular localization and protein interactions.. The GRX3 protein consists of 231 amino acids with a molecular weight of 25.54 kDa and a theoretical pI of 4.72.

    Virus:

    Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Micrococcal Nuclease New England Biolabs Cat# M0247S ATP Sigma-Aldrich Cat# 11140965001 Glutaraldehyde solution Sigma-Aldrich Cat# G7651 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat# DLM-4-PK NADH Sigma-Aldrich Cat# 10128023001 Phosphoenol-pyruvate Roche Cat# 10108294001 Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle Sigma-Aldrich Cat# P0294 SYBR Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) Thermo Fisher Scientific Cat# S11494 LabBlue Coomassie Brilliant Blue Rapid Staining Solution LABLEAD Cat# G1042 Cellfectin II Reagent Thermo Fisher Scientific Cat# 10362100 Sf-900 III SFM Thermo Fisher Scientific Cat# 12658019 DMEM, high glucose, pyruvate Gibco, Thermo Fisher Scientific Cat# 11995073 Fetal Bovine Serum, Value Gibco, Thermo Fisher Scientific Cat# A5256701 Penicillin-Streptomycin (10,000 U/mL) Gibco, Thermo Fisher Scientific Cat# 15140122 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–12.e1–e7, December 3, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin-EDTA (0.25%), phenol red Gibco, Thermo Fisher Scientific Cat# 25200072 PBS (10X), pH 7.4 Gibco, Thermo Fisher Scientific Cat# 70011044 UltraPure DNase/RNase-Free Distilled Water Invitrogen, Thermo Fisher Scientific Cat# 10977015 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Fisher Scientific Cat# 78440 Pierce BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23225 Immobilon -P PVDF Membrane Millipore Cat# IPVH00010 WesternBright ECL HRP substrate Advansta Cat# K-12045-D20 Cell Counting Kit-8 (CCK-8) MCE Cat# HY-K0301 Pyridostatin trihydrochloride (PDS) MCE Cat# HY-15176C Deposited data ATRX in complex with the nucleosome (consensus map) This study EMDB: EMD-62684 ATRX with the nucleosomal DNA (focus refinement map) This study EMDB: EMD-62686 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state (composite map) This study EMDB: EMD-62690 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state This study PDB: 9L06 Experimental models: Cell lines Sf9 cells in Sf-900 II SFM Thermo Fisher Scientific Cat#11496015

    Recombinant:

    Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Micrococcal Nuclease New England Biolabs Cat# M0247S ATP Sigma-Aldrich Cat# 11140965001 Glutaraldehyde solution Sigma-Aldrich Cat# G7651 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat# DLM-4-PK NADH Sigma-Aldrich Cat# 10128023001 Phosphoenol-pyruvate Roche Cat# 10108294001 Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle Sigma-Aldrich Cat# P0294 SYBR Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) Thermo Fisher Scientific Cat# S11494 LabBlue Coomassie Brilliant Blue Rapid Staining Solution LABLEAD Cat# G1042 Cellfectin II Reagent Thermo Fisher Scientific Cat# 10362100 Sf-900 III SFM Thermo Fisher Scientific Cat# 12658019 DMEM, high glucose, pyruvate Gibco, Thermo Fisher Scientific Cat# 11995073 Fetal Bovine Serum, Value Gibco, Thermo Fisher Scientific Cat# A5256701 Penicillin-Streptomycin (10,000 U/mL) Gibco, Thermo Fisher Scientific Cat# 15140122 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–12.e1–e7, December 3, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin-EDTA (0.25%), phenol red Gibco, Thermo Fisher Scientific Cat# 25200072 PBS (10X), pH 7.4 Gibco, Thermo Fisher Scientific Cat# 70011044 UltraPure DNase/RNase-Free Distilled Water Invitrogen, Thermo Fisher Scientific Cat# 10977015 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Fisher Scientific Cat# 78440 Pierce BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23225 Immobilon -P PVDF Membrane Millipore Cat# IPVH00010 WesternBright ECL HRP substrate Advansta Cat# K-12045-D20 Cell Counting Kit-8 (CCK-8) MCE Cat# HY-K0301 Pyridostatin trihydrochloride (PDS) MCE Cat# HY-15176C Deposited data ATRX in complex with the nucleosome (consensus map) This study EMDB: EMD-62684 ATRX with the nucleosomal DNA (focus refinement map) This study EMDB: EMD-62686 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state (composite map) This study EMDB: EMD-62690 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state This study PDB: 9L06 Experimental models: Cell lines Sf9 cells in Sf-900 II SFM Thermo Fisher Scientific Cat#11496015

    Article Title: Altered AMH signaling in Sertoli cells is associated with short photoperiod-induced testicular regression in Siberian hamsters.
    Article Snippet: Seasonal testicular regression is a key adaptive physiological process in seasonal breeding mammals, yet the changes in anti-Müllerian hormone (AMH) signaling associated with established testicular regression remain insufficiently understood.. In this study, we compared sexually mature Siberian hamsters maintained under long-, intermediate-, and short-photoperiod conditions for 8 weeks to characterize AMH-related changes after short photoperiod-induced testicular regression had been established.. Testicular morphology, AMH/AMHR2-SMAD signaling, and apoptosis-related parameters were examined.

    Protease Inhibitor:

    Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Micrococcal Nuclease New England Biolabs Cat# M0247S ATP Sigma-Aldrich Cat# 11140965001 Glutaraldehyde solution Sigma-Aldrich Cat# G7651 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat# DLM-4-PK NADH Sigma-Aldrich Cat# 10128023001 Phosphoenol-pyruvate Roche Cat# 10108294001 Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle Sigma-Aldrich Cat# P0294 SYBR Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) Thermo Fisher Scientific Cat# S11494 LabBlue Coomassie Brilliant Blue Rapid Staining Solution LABLEAD Cat# G1042 Cellfectin II Reagent Thermo Fisher Scientific Cat# 10362100 Sf-900 III SFM Thermo Fisher Scientific Cat# 12658019 DMEM, high glucose, pyruvate Gibco, Thermo Fisher Scientific Cat# 11995073 Fetal Bovine Serum, Value Gibco, Thermo Fisher Scientific Cat# A5256701 Penicillin-Streptomycin (10,000 U/mL) Gibco, Thermo Fisher Scientific Cat# 15140122 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–12.e1–e7, December 3, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin-EDTA (0.25%), phenol red Gibco, Thermo Fisher Scientific Cat# 25200072 PBS (10X), pH 7.4 Gibco, Thermo Fisher Scientific Cat# 70011044 UltraPure DNase/RNase-Free Distilled Water Invitrogen, Thermo Fisher Scientific Cat# 10977015 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Fisher Scientific Cat# 78440 Pierce BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23225 Immobilon -P PVDF Membrane Millipore Cat# IPVH00010 WesternBright ECL HRP substrate Advansta Cat# K-12045-D20 Cell Counting Kit-8 (CCK-8) MCE Cat# HY-K0301 Pyridostatin trihydrochloride (PDS) MCE Cat# HY-15176C Deposited data ATRX in complex with the nucleosome (consensus map) This study EMDB: EMD-62684 ATRX with the nucleosomal DNA (focus refinement map) This study EMDB: EMD-62686 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state (composite map) This study EMDB: EMD-62690 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state This study PDB: 9L06 Experimental models: Cell lines Sf9 cells in Sf-900 II SFM Thermo Fisher Scientific Cat#11496015

    Cloning:

    Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Micrococcal Nuclease New England Biolabs Cat# M0247S ATP Sigma-Aldrich Cat# 11140965001 Glutaraldehyde solution Sigma-Aldrich Cat# G7651 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat# DLM-4-PK NADH Sigma-Aldrich Cat# 10128023001 Phosphoenol-pyruvate Roche Cat# 10108294001 Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle Sigma-Aldrich Cat# P0294 SYBR Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) Thermo Fisher Scientific Cat# S11494 LabBlue Coomassie Brilliant Blue Rapid Staining Solution LABLEAD Cat# G1042 Cellfectin II Reagent Thermo Fisher Scientific Cat# 10362100 Sf-900 III SFM Thermo Fisher Scientific Cat# 12658019 DMEM, high glucose, pyruvate Gibco, Thermo Fisher Scientific Cat# 11995073 Fetal Bovine Serum, Value Gibco, Thermo Fisher Scientific Cat# A5256701 Penicillin-Streptomycin (10,000 U/mL) Gibco, Thermo Fisher Scientific Cat# 15140122 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–12.e1–e7, December 3, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin-EDTA (0.25%), phenol red Gibco, Thermo Fisher Scientific Cat# 25200072 PBS (10X), pH 7.4 Gibco, Thermo Fisher Scientific Cat# 70011044 UltraPure DNase/RNase-Free Distilled Water Invitrogen, Thermo Fisher Scientific Cat# 10977015 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Fisher Scientific Cat# 78440 Pierce BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23225 Immobilon -P PVDF Membrane Millipore Cat# IPVH00010 WesternBright ECL HRP substrate Advansta Cat# K-12045-D20 Cell Counting Kit-8 (CCK-8) MCE Cat# HY-K0301 Pyridostatin trihydrochloride (PDS) MCE Cat# HY-15176C Deposited data ATRX in complex with the nucleosome (consensus map) This study EMDB: EMD-62684 ATRX with the nucleosomal DNA (focus refinement map) This study EMDB: EMD-62686 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state (composite map) This study EMDB: EMD-62690 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state This study PDB: 9L06 Experimental models: Cell lines Sf9 cells in Sf-900 II SFM Thermo Fisher Scientific Cat#11496015

    Gel Extraction:

    Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Micrococcal Nuclease New England Biolabs Cat# M0247S ATP Sigma-Aldrich Cat# 11140965001 Glutaraldehyde solution Sigma-Aldrich Cat# G7651 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat# DLM-4-PK NADH Sigma-Aldrich Cat# 10128023001 Phosphoenol-pyruvate Roche Cat# 10108294001 Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle Sigma-Aldrich Cat# P0294 SYBR Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) Thermo Fisher Scientific Cat# S11494 LabBlue Coomassie Brilliant Blue Rapid Staining Solution LABLEAD Cat# G1042 Cellfectin II Reagent Thermo Fisher Scientific Cat# 10362100 Sf-900 III SFM Thermo Fisher Scientific Cat# 12658019 DMEM, high glucose, pyruvate Gibco, Thermo Fisher Scientific Cat# 11995073 Fetal Bovine Serum, Value Gibco, Thermo Fisher Scientific Cat# A5256701 Penicillin-Streptomycin (10,000 U/mL) Gibco, Thermo Fisher Scientific Cat# 15140122 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–12.e1–e7, December 3, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin-EDTA (0.25%), phenol red Gibco, Thermo Fisher Scientific Cat# 25200072 PBS (10X), pH 7.4 Gibco, Thermo Fisher Scientific Cat# 70011044 UltraPure DNase/RNase-Free Distilled Water Invitrogen, Thermo Fisher Scientific Cat# 10977015 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Fisher Scientific Cat# 78440 Pierce BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23225 Immobilon -P PVDF Membrane Millipore Cat# IPVH00010 WesternBright ECL HRP substrate Advansta Cat# K-12045-D20 Cell Counting Kit-8 (CCK-8) MCE Cat# HY-K0301 Pyridostatin trihydrochloride (PDS) MCE Cat# HY-15176C Deposited data ATRX in complex with the nucleosome (consensus map) This study EMDB: EMD-62684 ATRX with the nucleosomal DNA (focus refinement map) This study EMDB: EMD-62686 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state (composite map) This study EMDB: EMD-62690 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state This study PDB: 9L06 Experimental models: Cell lines Sf9 cells in Sf-900 II SFM Thermo Fisher Scientific Cat#11496015

    Article Title: Generation and Preliminary Characterization of a DsRed-MBD Microtubule Reporter Line in Ipomoea cordatotriloba
    Article Snippet: The pCAMBIA1300 vector was linearized by double digestion with Xba I and Sac I (New England Biolabs, R3142S, Ipswich, MA, USA) at 37 ◦C. .. The target linear vector band was recovered and gel-purified using a Gel Extraction Kit (Vazyme, DC201-01, Nanjing, China) after size verification by agarose gel electrophoresis. ..

    Purification:

    Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Micrococcal Nuclease New England Biolabs Cat# M0247S ATP Sigma-Aldrich Cat# 11140965001 Glutaraldehyde solution Sigma-Aldrich Cat# G7651 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat# DLM-4-PK NADH Sigma-Aldrich Cat# 10128023001 Phosphoenol-pyruvate Roche Cat# 10108294001 Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle Sigma-Aldrich Cat# P0294 SYBR Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) Thermo Fisher Scientific Cat# S11494 LabBlue Coomassie Brilliant Blue Rapid Staining Solution LABLEAD Cat# G1042 Cellfectin II Reagent Thermo Fisher Scientific Cat# 10362100 Sf-900 III SFM Thermo Fisher Scientific Cat# 12658019 DMEM, high glucose, pyruvate Gibco, Thermo Fisher Scientific Cat# 11995073 Fetal Bovine Serum, Value Gibco, Thermo Fisher Scientific Cat# A5256701 Penicillin-Streptomycin (10,000 U/mL) Gibco, Thermo Fisher Scientific Cat# 15140122 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–12.e1–e7, December 3, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin-EDTA (0.25%), phenol red Gibco, Thermo Fisher Scientific Cat# 25200072 PBS (10X), pH 7.4 Gibco, Thermo Fisher Scientific Cat# 70011044 UltraPure DNase/RNase-Free Distilled Water Invitrogen, Thermo Fisher Scientific Cat# 10977015 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Fisher Scientific Cat# 78440 Pierce BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23225 Immobilon -P PVDF Membrane Millipore Cat# IPVH00010 WesternBright ECL HRP substrate Advansta Cat# K-12045-D20 Cell Counting Kit-8 (CCK-8) MCE Cat# HY-K0301 Pyridostatin trihydrochloride (PDS) MCE Cat# HY-15176C Deposited data ATRX in complex with the nucleosome (consensus map) This study EMDB: EMD-62684 ATRX with the nucleosomal DNA (focus refinement map) This study EMDB: EMD-62686 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state (composite map) This study EMDB: EMD-62690 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state This study PDB: 9L06 Experimental models: Cell lines Sf9 cells in Sf-900 II SFM Thermo Fisher Scientific Cat#11496015

    DNA Extraction:

    Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Micrococcal Nuclease New England Biolabs Cat# M0247S ATP Sigma-Aldrich Cat# 11140965001 Glutaraldehyde solution Sigma-Aldrich Cat# G7651 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat# DLM-4-PK NADH Sigma-Aldrich Cat# 10128023001 Phosphoenol-pyruvate Roche Cat# 10108294001 Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle Sigma-Aldrich Cat# P0294 SYBR Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) Thermo Fisher Scientific Cat# S11494 LabBlue Coomassie Brilliant Blue Rapid Staining Solution LABLEAD Cat# G1042 Cellfectin II Reagent Thermo Fisher Scientific Cat# 10362100 Sf-900 III SFM Thermo Fisher Scientific Cat# 12658019 DMEM, high glucose, pyruvate Gibco, Thermo Fisher Scientific Cat# 11995073 Fetal Bovine Serum, Value Gibco, Thermo Fisher Scientific Cat# A5256701 Penicillin-Streptomycin (10,000 U/mL) Gibco, Thermo Fisher Scientific Cat# 15140122 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–12.e1–e7, December 3, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin-EDTA (0.25%), phenol red Gibco, Thermo Fisher Scientific Cat# 25200072 PBS (10X), pH 7.4 Gibco, Thermo Fisher Scientific Cat# 70011044 UltraPure DNase/RNase-Free Distilled Water Invitrogen, Thermo Fisher Scientific Cat# 10977015 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Fisher Scientific Cat# 78440 Pierce BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23225 Immobilon -P PVDF Membrane Millipore Cat# IPVH00010 WesternBright ECL HRP substrate Advansta Cat# K-12045-D20 Cell Counting Kit-8 (CCK-8) MCE Cat# HY-K0301 Pyridostatin trihydrochloride (PDS) MCE Cat# HY-15176C Deposited data ATRX in complex with the nucleosome (consensus map) This study EMDB: EMD-62684 ATRX with the nucleosomal DNA (focus refinement map) This study EMDB: EMD-62686 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state (composite map) This study EMDB: EMD-62690 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state This study PDB: 9L06 Experimental models: Cell lines Sf9 cells in Sf-900 II SFM Thermo Fisher Scientific Cat#11496015

    Staining:

    Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies ATRX Polyclonal antibody Proteintech Cat# 20495-1-AP;RRID: AB_3085606 β-Actin Mouse Monoclonal Antibody (HRP Conjugated) Beyotime, Cat# AF2815 Goat Anti-Rabbit Immunoglobulins/HRP Agilent Cat#P044801;RRID: AB_2617138 Bacterial and virus strains DH10Bac Competent Cells Thermo Fisher Scientific Cat# 10361012 DH5α Competent Cells Thermo Fisher Scientific Cat# 18258012 Stbl3 Competent Cells Thermo Fisher Scientific Cat# C737303 JM109 Competent Cells Fisher Scientific Cat# PR-L1001 Chemicals, peptides, and recombinant proteins Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) MCE Cat# HY-K0011 DNase I New England Biolabs Cat# M0303L Nickel agarose beads GOLDBIO Cat# H-320 Quick CIP (DNA Modifying Enzymes and Cloning Technologies) New England Biolabs Cat# M0525L Beryllium sulfate tetrahydrate Sigma-Aldrich Cat# 202789 Adenosine 5′-diphosphate Sigma-Aldrich Cat# 01905 UltraPure Glycerol Thermo Fisher Scientific Cat# 15514011 Sodium fluoride Sigma-Aldrich Cat# 201154 UltraPure DNase/RNase-Free Distilled Water Thermo Fisher Scientific Cat# 10977015 DTT (dithiothreitol) Thermo Fisher Scientific Cat# R0862 Benzonase Nuclease Millipore Cat# E1014 Phusion High-Fidelity DNA Polymerase NEB Cat# M0530L MinElute Gel Extraction Kit QIAGEN Cat# 28604 TaKaRa MiniBEST DNA Fragment Purification Kit TaKaRa Cat# 9761 FastPure Plasmid Mini Kit Vazyme Cat# DC201-01 FastPure Gel DNA Extraction Mini Kit Vazyme Cat# DC301-01 FastPure Cell/Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 Micrococcal Nuclease New England Biolabs Cat# M0247S ATP Sigma-Aldrich Cat# 11140965001 Glutaraldehyde solution Sigma-Aldrich Cat# G7651 Deuterium oxide (D, 99.9%) Cambridge Isotope Laboratories Cat# DLM-4-PK NADH Sigma-Aldrich Cat# 10128023001 Phosphoenol-pyruvate Roche Cat# 10108294001 Pyruvate Kinase/Lactic Dehydrogenase enzymes from rabbit muscle Sigma-Aldrich Cat# P0294 SYBR Gold Nucleic Acid Gel Stain (10,000X Concentrate in DMSO) Thermo Fisher Scientific Cat# S11494 LabBlue Coomassie Brilliant Blue Rapid Staining Solution LABLEAD Cat# G1042 Cellfectin II Reagent Thermo Fisher Scientific Cat# 10362100 Sf-900 III SFM Thermo Fisher Scientific Cat# 12658019 DMEM, high glucose, pyruvate Gibco, Thermo Fisher Scientific Cat# 11995073 Fetal Bovine Serum, Value Gibco, Thermo Fisher Scientific Cat# A5256701 Penicillin-Streptomycin (10,000 U/mL) Gibco, Thermo Fisher Scientific Cat# 15140122 (Continued on next page) ll OPEN ACCESSArticle Molecular Cell 86, 1–12.e1–e7, December 3, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Trypsin-EDTA (0.25%), phenol red Gibco, Thermo Fisher Scientific Cat# 25200072 PBS (10X), pH 7.4 Gibco, Thermo Fisher Scientific Cat# 70011044 UltraPure DNase/RNase-Free Distilled Water Invitrogen, Thermo Fisher Scientific Cat# 10977015 RIPA Lysis and Extraction Buffer Thermo Fisher Scientific Cat# 89900 Halt Protease and Phosphatase Inhibitor Cocktail (100X) Thermo Fisher Scientific Cat# 78440 Pierce BCA Protein Assay Kits Thermo Fisher Scientific Cat# 23225 Immobilon -P PVDF Membrane Millipore Cat# IPVH00010 WesternBright ECL HRP substrate Advansta Cat# K-12045-D20 Cell Counting Kit-8 (CCK-8) MCE Cat# HY-K0301 Pyridostatin trihydrochloride (PDS) MCE Cat# HY-15176C Deposited data ATRX in complex with the nucleosome (consensus map) This study EMDB: EMD-62684 ATRX with the nucleosomal DNA (focus refinement map) This study EMDB: EMD-62686 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state (composite map) This study EMDB: EMD-62690 The cryo-EM structure of ATRX in complex with the nucleosome in the ADP⋅BeF3-bound state This study PDB: 9L06 Experimental models: Cell lines Sf9 cells in Sf-900 II SFM Thermo Fisher Scientific Cat#11496015

    Concentration Assay:

    Article Title: Heatwave-Resilient Cuticular Microbiota Protect the Leaf Beetle Plagiodera versicolora From Fungal Infection.
    Article Snippet: .. Plasmid DNA was extracted using the FastPure Plasmid Mini Kit (Vazyme Biotech Co., Ltd., China), and its concentration was measured with a Nanodrop ND ‐2000 spectrophotometer (Nanodrop, USA). ..

    Spectrophotometry:

    Article Title: Heatwave-Resilient Cuticular Microbiota Protect the Leaf Beetle Plagiodera versicolora From Fungal Infection.
    Article Snippet: .. Plasmid DNA was extracted using the FastPure Plasmid Mini Kit (Vazyme Biotech Co., Ltd., China), and its concentration was measured with a Nanodrop ND ‐2000 spectrophotometer (Nanodrop, USA). ..

    Agarose Gel Electrophoresis:

    Article Title: Generation and Preliminary Characterization of a DsRed-MBD Microtubule Reporter Line in Ipomoea cordatotriloba
    Article Snippet: The pCAMBIA1300 vector was linearized by double digestion with Xba I and Sac I (New England Biolabs, R3142S, Ipswich, MA, USA) at 37 ◦C. .. The target linear vector band was recovered and gel-purified using a Gel Extraction Kit (Vazyme, DC201-01, Nanjing, China) after size verification by agarose gel electrophoresis. ..



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    Image Search Results


    Journal: iScience

    Article Title: Rapid screening of ancient DNA extracts through uracil detection

    doi: 10.1016/j.isci.2026.117820

    Figure Lengend Snippet:

    Article Snippet: DNA were isolated using the GeneJET Genomic DNA Purification kit (K0721, Thermo Fisher Scientific, USA), the NucleoSpin Plasmid Mini kit (740588.250, Macherey-Nagel, Germany) and the Quick-DNA Miniprep Plus kit (D4068, Zymo Research, USA), from P. major , plasmid, and E. coli samples respectively, all following the manufacturers’ protocols.

    Techniques: Plasmid Preparation