Clinical Proteomics:Article Title: Prolonged intratumoral treatment with TLR7/8 agonist R848 regulates the tumor immune microenvironment resulting in enhanced antitumor activity
Article Snippet: .. To assess the dynamics of R848 levels in tumor and plasma following prolonged intratumoral injection, 708 tumor-bearing mice were treated with intratumoral injection of 50 μL phosphate buffer solution (PBS, Procell, Cat. # PB180327 ) for the sham group, with R848 (InvivoGen, Cat. # tlrl-r848) dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a syringe (50 μL PBS) for the regular intratumoral injection group, or with R848 dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a micropump system (InnoPump, China) (500 μL PBS) for prolonged intratumoral injection over 25 h. Mice were given standard fodder and sterile drinking water, with weight and tumor size recorded every seven days. ..
Article Title: Prolonged intratumoral treatment with TLR7/8 agonist R848 regulates the tumor immune microenvironment resulting in enhanced antitumor activity.
Article Snippet: .. To assess the dynamics of R848 levels in tumor and plasma following prolonged intratumoral injection, 708 tumor-bearing mice were treated with intratumoral injection of 50 μL phosphate buffer solution (PBS, Procell, Cat. # PB180327) for the sham group, with R848 (InvivoGen, Cat. # tlrl-r848) dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a syringe (50 μL PBS) for the regular intratumoral injection group, or with R848 dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a micropump system (InnoPump, China) (500 μL PBS) for prolonged intratumoral injection over 25 h. Mice were given standard fodder and sterile drinking water, with weight and tumor size recorded every seven days. ..
Injection:Article Title: Prolonged intratumoral treatment with TLR7/8 agonist R848 regulates the tumor immune microenvironment resulting in enhanced antitumor activity
Article Snippet: .. To assess the dynamics of R848 levels in tumor and plasma following prolonged intratumoral injection, 708 tumor-bearing mice were treated with intratumoral injection of 50 μL phosphate buffer solution (PBS, Procell, Cat. # PB180327 ) for the sham group, with R848 (InvivoGen, Cat. # tlrl-r848) dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a syringe (50 μL PBS) for the regular intratumoral injection group, or with R848 dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a micropump system (InnoPump, China) (500 μL PBS) for prolonged intratumoral injection over 25 h. Mice were given standard fodder and sterile drinking water, with weight and tumor size recorded every seven days. ..
Article Title: Prolonged intratumoral treatment with TLR7/8 agonist R848 regulates the tumor immune microenvironment resulting in enhanced antitumor activity.
Article Snippet: .. To assess the dynamics of R848 levels in tumor and plasma following prolonged intratumoral injection, 708 tumor-bearing mice were treated with intratumoral injection of 50 μL phosphate buffer solution (PBS, Procell, Cat. # PB180327) for the sham group, with R848 (InvivoGen, Cat. # tlrl-r848) dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a syringe (50 μL PBS) for the regular intratumoral injection group, or with R848 dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a micropump system (InnoPump, China) (500 μL PBS) for prolonged intratumoral injection over 25 h. Mice were given standard fodder and sterile drinking water, with weight and tumor size recorded every seven days. ..
Mouse Assay:Article Title: Prolonged intratumoral treatment with TLR7/8 agonist R848 regulates the tumor immune microenvironment resulting in enhanced antitumor activity
Article Snippet: .. To assess the dynamics of R848 levels in tumor and plasma following prolonged intratumoral injection, 708 tumor-bearing mice were treated with intratumoral injection of 50 μL phosphate buffer solution (PBS, Procell, Cat. # PB180327 ) for the sham group, with R848 (InvivoGen, Cat. # tlrl-r848) dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a syringe (50 μL PBS) for the regular intratumoral injection group, or with R848 dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a micropump system (InnoPump, China) (500 μL PBS) for prolonged intratumoral injection over 25 h. Mice were given standard fodder and sterile drinking water, with weight and tumor size recorded every seven days. ..
Article Title: Prolonged intratumoral treatment with TLR7/8 agonist R848 regulates the tumor immune microenvironment resulting in enhanced antitumor activity.
Article Snippet: .. To assess the dynamics of R848 levels in tumor and plasma following prolonged intratumoral injection, 708 tumor-bearing mice were treated with intratumoral injection of 50 μL phosphate buffer solution (PBS, Procell, Cat. # PB180327) for the sham group, with R848 (InvivoGen, Cat. # tlrl-r848) dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a syringe (50 μL PBS) for the regular intratumoral injection group, or with R848 dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a micropump system (InnoPump, China) (500 μL PBS) for prolonged intratumoral injection over 25 h. Mice were given standard fodder and sterile drinking water, with weight and tumor size recorded every seven days. ..
Sterility:Article Title: Prolonged intratumoral treatment with TLR7/8 agonist R848 regulates the tumor immune microenvironment resulting in enhanced antitumor activity
Article Snippet: .. To assess the dynamics of R848 levels in tumor and plasma following prolonged intratumoral injection, 708 tumor-bearing mice were treated with intratumoral injection of 50 μL phosphate buffer solution (PBS, Procell, Cat. # PB180327 ) for the sham group, with R848 (InvivoGen, Cat. # tlrl-r848) dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a syringe (50 μL PBS) for the regular intratumoral injection group, or with R848 dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a micropump system (InnoPump, China) (500 μL PBS) for prolonged intratumoral injection over 25 h. Mice were given standard fodder and sterile drinking water, with weight and tumor size recorded every seven days. ..
Article Title: Prolonged intratumoral treatment with TLR7/8 agonist R848 regulates the tumor immune microenvironment resulting in enhanced antitumor activity.
Article Snippet: .. To assess the dynamics of R848 levels in tumor and plasma following prolonged intratumoral injection, 708 tumor-bearing mice were treated with intratumoral injection of 50 μL phosphate buffer solution (PBS, Procell, Cat. # PB180327) for the sham group, with R848 (InvivoGen, Cat. # tlrl-r848) dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a syringe (50 μL PBS) for the regular intratumoral injection group, or with R848 dissolved at different doses (200 ng, 1 μg, 5 μg and 10 μg) via a micropump system (InnoPump, China) (500 μL PBS) for prolonged intratumoral injection over 25 h. Mice were given standard fodder and sterile drinking water, with weight and tumor size recorded every seven days. ..
other:
Staining:
Incubation:
Cytotoxicity Assay:Article Title: Amelioration of acute lung injury by Salvia miltiorrhiza -derived extracellular vesicles: through repair of the vascular barrier and modulation of lung microbiota
Article Snippet: .. The reagents used in this study include: DMEM basal medium (Procell, WHB824D281), phosphate buffer solution (Procell, WHB824A031), and penicillin–streptomycin mixture (Procell, WH1021A161); fetal bovine serum (Thermo Fisher, SA210518); cell-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2630, 0000155608) and animal-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2800, 0000189843); CCK8 cell proliferation and cytotoxicity assay kit (Biosharp, BS350A); BCA protein quantification kit (Lianke Biotech, 81910335A); mRNA reverse transcription primers and PCR (real-time quantitative polymerase chain reaction, RT-qPCR) primers (synthesized by Shanghai Sangon Biotech Co., Ltd.); CD81 antibody (Servicebio, GB113151 ), VE-cadherin antibody (Servicebio, GB14013), fluorescein isothiocyanate isomer (FITC)-labeled donkey anti-mouse secondary antibody (Servicebio, GB22401), and CY3-labeled goat anti-rabbit secondary antibody (Servicebio, GB21303); 4′,6-diamidino-2-phenylindole (DAPI) solution and tetramethylrhodamine isothiocyanate (rhodamine phalloidin) (Beijing Solarbio Science & Technology Co., Ltd.). ..
Reverse Transcription:Article Title: Amelioration of acute lung injury by Salvia miltiorrhiza -derived extracellular vesicles: through repair of the vascular barrier and modulation of lung microbiota
Article Snippet: .. The reagents used in this study include: DMEM basal medium (Procell, WHB824D281), phosphate buffer solution (Procell, WHB824A031), and penicillin–streptomycin mixture (Procell, WH1021A161); fetal bovine serum (Thermo Fisher, SA210518); cell-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2630, 0000155608) and animal-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2800, 0000189843); CCK8 cell proliferation and cytotoxicity assay kit (Biosharp, BS350A); BCA protein quantification kit (Lianke Biotech, 81910335A); mRNA reverse transcription primers and PCR (real-time quantitative polymerase chain reaction, RT-qPCR) primers (synthesized by Shanghai Sangon Biotech Co., Ltd.); CD81 antibody (Servicebio, GB113151 ), VE-cadherin antibody (Servicebio, GB14013), fluorescein isothiocyanate isomer (FITC)-labeled donkey anti-mouse secondary antibody (Servicebio, GB22401), and CY3-labeled goat anti-rabbit secondary antibody (Servicebio, GB21303); 4′,6-diamidino-2-phenylindole (DAPI) solution and tetramethylrhodamine isothiocyanate (rhodamine phalloidin) (Beijing Solarbio Science & Technology Co., Ltd.). ..
Polymerase Chain Reaction:Article Title: Amelioration of acute lung injury by Salvia miltiorrhiza -derived extracellular vesicles: through repair of the vascular barrier and modulation of lung microbiota
Article Snippet: .. The reagents used in this study include: DMEM basal medium (Procell, WHB824D281), phosphate buffer solution (Procell, WHB824A031), and penicillin–streptomycin mixture (Procell, WH1021A161); fetal bovine serum (Thermo Fisher, SA210518); cell-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2630, 0000155608) and animal-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2800, 0000189843); CCK8 cell proliferation and cytotoxicity assay kit (Biosharp, BS350A); BCA protein quantification kit (Lianke Biotech, 81910335A); mRNA reverse transcription primers and PCR (real-time quantitative polymerase chain reaction, RT-qPCR) primers (synthesized by Shanghai Sangon Biotech Co., Ltd.); CD81 antibody (Servicebio, GB113151 ), VE-cadherin antibody (Servicebio, GB14013), fluorescein isothiocyanate isomer (FITC)-labeled donkey anti-mouse secondary antibody (Servicebio, GB22401), and CY3-labeled goat anti-rabbit secondary antibody (Servicebio, GB21303); 4′,6-diamidino-2-phenylindole (DAPI) solution and tetramethylrhodamine isothiocyanate (rhodamine phalloidin) (Beijing Solarbio Science & Technology Co., Ltd.). ..
Real-time Polymerase Chain Reaction:Article Title: Amelioration of acute lung injury by Salvia miltiorrhiza -derived extracellular vesicles: through repair of the vascular barrier and modulation of lung microbiota
Article Snippet: .. The reagents used in this study include: DMEM basal medium (Procell, WHB824D281), phosphate buffer solution (Procell, WHB824A031), and penicillin–streptomycin mixture (Procell, WH1021A161); fetal bovine serum (Thermo Fisher, SA210518); cell-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2630, 0000155608) and animal-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2800, 0000189843); CCK8 cell proliferation and cytotoxicity assay kit (Biosharp, BS350A); BCA protein quantification kit (Lianke Biotech, 81910335A); mRNA reverse transcription primers and PCR (real-time quantitative polymerase chain reaction, RT-qPCR) primers (synthesized by Shanghai Sangon Biotech Co., Ltd.); CD81 antibody (Servicebio, GB113151 ), VE-cadherin antibody (Servicebio, GB14013), fluorescein isothiocyanate isomer (FITC)-labeled donkey anti-mouse secondary antibody (Servicebio, GB22401), and CY3-labeled goat anti-rabbit secondary antibody (Servicebio, GB21303); 4′,6-diamidino-2-phenylindole (DAPI) solution and tetramethylrhodamine isothiocyanate (rhodamine phalloidin) (Beijing Solarbio Science & Technology Co., Ltd.). ..
Synthesized:Article Title: Amelioration of acute lung injury by Salvia miltiorrhiza -derived extracellular vesicles: through repair of the vascular barrier and modulation of lung microbiota
Article Snippet: .. The reagents used in this study include: DMEM basal medium (Procell, WHB824D281), phosphate buffer solution (Procell, WHB824A031), and penicillin–streptomycin mixture (Procell, WH1021A161); fetal bovine serum (Thermo Fisher, SA210518); cell-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2630, 0000155608) and animal-specific lipopolysaccharide (Sigma-Aldrich, LPS-L2800, 0000189843); CCK8 cell proliferation and cytotoxicity assay kit (Biosharp, BS350A); BCA protein quantification kit (Lianke Biotech, 81910335A); mRNA reverse transcription primers and PCR (real-time quantitative polymerase chain reaction, RT-qPCR) primers (synthesized by Shanghai Sangon Biotech Co., Ltd.); CD81 antibody (Servicebio, GB113151 ), VE-cadherin antibody (Servicebio, GB14013), fluorescein isothiocyanate isomer (FITC)-labeled donkey anti-mouse secondary antibody (Servicebio, GB22401), and CY3-labeled goat anti-rabbit secondary antibody (Servicebio, GB21303); 4′,6-diamidino-2-phenylindole (DAPI) solution and tetramethylrhodamine isothiocyanate (rhodamine phalloidin) (Beijing Solarbio Science & Technology Co., Ltd.). ..
|
