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paraformaldehyde phosphate buffer solution  (Nacalai)

 
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    Structured Review

    Nacalai paraformaldehyde phosphate buffer solution
    Paraformaldehyde Phosphate Buffer Solution, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphate+buffer+solution/buffer+paraformaldehyde+phosphate+solution/pm42321187-525-11-15
    Average 86 stars, based on 1 article reviews
    paraformaldehyde phosphate buffer solution - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    other:

    Article Title: PLGA Nanoparticle-Mediated Sustained Release of Fisetin for Intra-Articular Therapy of Temporomandibular Joint Osteoarthritis
    Article Snippet: Samples were fixed in 4% paraformaldehyde phosphate buffer (Nacalai Tesque Co., Ltd.) at 4 °C.

    Saline:

    Article Title: In vitro inhibition of Escherichia coli β-glucuronidase by aripiprazole.
    Article Snippet: .. Phosphate buffer (PB; pH7.0; 37,242–55), phosphate-buffered saline (PBS; 14,249–24), protease inhibitors (25955–11), and purified GUS (UniProt ID: P05804; 16,944–02) were acquired from NACALAI TESQUE, INC (Kyoto, Japan). .. Escherichia coli K-12 (E. coli) were purchased from the National BioResource Project (NBRP, Shizuoka, Japan).

    Article Title: Characterization of novel macrophage cell lines derived from goat and sheep blood and their susceptibility to ruminant virus infections.
    Article Snippet: Cells were seeded on eight-well chamber slides (Asahi Glass Co., Ltd., Tokyo, Japan) at a density of 2 × 105 cells/well. .. The next day, after being washed once with Dul becco’s phosphate-bu ered saline (DPBS), the cells were xed using 4% paraformalde hyde phosphate bu er solution (Nacalai Tesque), permeabilized with 1% Triton X-100/ phosphate-bu ered saline solution, and blocked with Blocking One Histo (Nacalai Tesque). .. Cells were then incubated with the primary antibodies at room temperature for 1 h, and the EnVision system (DAKO, Hamburg, Germany) was used to visualize antibody-antigen reactions, according to the manufacturer’s procedure.

    Purification:

    Article Title: In vitro inhibition of Escherichia coli β-glucuronidase by aripiprazole.
    Article Snippet: .. Phosphate buffer (PB; pH7.0; 37,242–55), phosphate-buffered saline (PBS; 14,249–24), protease inhibitors (25955–11), and purified GUS (UniProt ID: P05804; 16,944–02) were acquired from NACALAI TESQUE, INC (Kyoto, Japan). .. Escherichia coli K-12 (E. coli) were purchased from the National BioResource Project (NBRP, Shizuoka, Japan).

    Immunocytochemistry:

    Article Title: Blood IL-6 is a critical trigger of depressive symptoms in a mouse model for human atopic dermatitis.
    Article Snippet: .. Immunocytochemistry Neuronal progenitor cells (NPCs)/well were fixed with 200 l of 4% paraformaldehyde phosphate buffer solution (Nacalai Tesque, Kyoto, Japan, 09154-85) for 20 min at room temperature. .. After washing with PBS twice, the cells were treated with blocking reagent II (Nichirei Bioscience, Tokyo, Japan, 426042) for 1 h. To detect IL-6R on the cell surface, samples were incubated with 100 l of 1 Ab mixture [rabbit anti-human/mouse IL-6R polyclonal Ab (1:300; Invitrogen, PA5102425) and rat anti-mouse gp130 mAb (R&D Systems, Clone 125623, MAB4681)] overnight at 4°C, followed by 100 l of 2 Ab mixture [goat anti-rabbit IgG (H+L) cross-adsorbed secondary Ab conjugated with Alexa Fluor 594 (1:1,000; Invitrogen, A11012) and goat anti-rat IgG (H+L) cross-adsorbed secondary Ab conjugated with Alexa Fluor 488 (1:1,000; Invitrogen, A11006)] for 1 h at room temperature in the dark.

    Infection:

    Article Title: Virological characteristics of SARS-CoV-2-related coronaviruses dynamically circulating in Southeast Asia.
    Article Snippet: .. 39,40,47 In brief, one day before infection, VeroE6/TMPRSS2 cells (200,000 cells) were seeded into a 12-well plate and infected with rSARS-CoV-2, rBANAL-20-236, and rRacCS20637 (MOI 0.01) at 37 ◦ C. At 2 h.p.i. mounting solution containing 3% FBS and 1.5% carboxymethyl cellulose (Wako, cat# 039-01335) was overlaid, followed by incubation at 37 ◦ C. At 3 d.p.i., the culture medium was removed, and the cells were washed with PBS three times and fixed with 4% paraformaldehyde phosphate (Nacalai Tesque, cat# 09154-85). .. The fixed cells were washed with tap water, dried and stained with staining solution (0.1% methylene blue (Nacalai Tesque, cat# 22412-14) in water) for 30 minutes.

    Incubation:

    Article Title: Virological characteristics of SARS-CoV-2-related coronaviruses dynamically circulating in Southeast Asia.
    Article Snippet: .. 39,40,47 In brief, one day before infection, VeroE6/TMPRSS2 cells (200,000 cells) were seeded into a 12-well plate and infected with rSARS-CoV-2, rBANAL-20-236, and rRacCS20637 (MOI 0.01) at 37 ◦ C. At 2 h.p.i. mounting solution containing 3% FBS and 1.5% carboxymethyl cellulose (Wako, cat# 039-01335) was overlaid, followed by incubation at 37 ◦ C. At 3 d.p.i., the culture medium was removed, and the cells were washed with PBS three times and fixed with 4% paraformaldehyde phosphate (Nacalai Tesque, cat# 09154-85). .. The fixed cells were washed with tap water, dried and stained with staining solution (0.1% methylene blue (Nacalai Tesque, cat# 22412-14) in water) for 30 minutes.

    Blocking Assay:

    Article Title: Characterization of novel macrophage cell lines derived from goat and sheep blood and their susceptibility to ruminant virus infections.
    Article Snippet: Cells were seeded on eight-well chamber slides (Asahi Glass Co., Ltd., Tokyo, Japan) at a density of 2 × 105 cells/well. .. The next day, after being washed once with Dul becco’s phosphate-bu ered saline (DPBS), the cells were xed using 4% paraformalde hyde phosphate bu er solution (Nacalai Tesque), permeabilized with 1% Triton X-100/ phosphate-bu ered saline solution, and blocked with Blocking One Histo (Nacalai Tesque). .. Cells were then incubated with the primary antibodies at room temperature for 1 h, and the EnVision system (DAKO, Hamburg, Germany) was used to visualize antibody-antigen reactions, according to the manufacturer’s procedure.



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