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pgl3 basic vector  (Addgene inc)


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    Structured Review

    Addgene inc pgl3 basic vector
    Pgl3 Basic Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3-basic+luciferase+expression+vector/pGL3+(-BRE)+Luciferase+(Plasmid+%2345127)/pmc04893643-69-17-19
    Average 95 stars, based on 195 article reviews
    pgl3 basic vector - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Method for preparing
    Article Snippet: .. COS7 monkey kidney cells (ATCC) were cultured on a 24-well plate at 1.5×105 cells/well and then a pGL3-PGC-1α-Luc plasm id (Addgene, Cambridge, MA, USA) was transfected into the cells using Lipofector (Aptabio, Yongin, Korea). ..

    Transfection:

    Article Title: Method for preparing
    Article Snippet: .. COS7 monkey kidney cells (ATCC) were cultured on a 24-well plate at 1.5×105 cells/well and then a pGL3-PGC-1α-Luc plasm id (Addgene, Cambridge, MA, USA) was transfected into the cells using Lipofector (Aptabio, Yongin, Korea). ..

    Plasmid Preparation:

    Article Title: The natural L370F ERα variant confers endocrine resistance and sensitivity to ATRA in metastatic breast cancer cells
    Article Snippet: .. To generate the plasmid pGL2Basic_Neo_RARE-NLuc-PEST, the RARE cassette was KpnI and HindIII excised from the pGL3-RARE-luciferase [ ] purchased from Addgene (USA) and ligated into the corresponding restriction sites in the pGL2Basic_Neo_NLuc-PEST [ , ]. ..

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: The natural L370F ERα variant confers endocrine resistance and sensitivity to ATRA in metastatic breast cancer cells.
    Article Snippet: .. To generate the plasmid pGL2Basic_Neo_RARE-NLuc-PEST, the RARE cassette was KpnI and HindIII excised from the pGL3-RAREluciferase [25] purchased from Addgene (USA) and ligated into the corresponding restriction sites in the pGL2Basic_Neo_NLuc-PEST [22, 23]. ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
    Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the pGL3-Basic 180 luciferase reporter plasmid (RRID:Addgene_212936). ..

    Article Title: Ebselen Suppresses Breast Cancer Tumorigenesis by Inhibiting YTHDF1-Mediated c-Fos Expression.
    Article Snippet: To design the translation reporter construct, the putative m6A modification site of FOS (chr4:5278828–75282230) was PCR-amplified from MCF7 cDNA and cloned into the 3′-UTR of RLuc at the XhoI site of the psiCHECK3 vector (a gift from Anthony Leung; Addgene plasmid #136010). .. The pGL3-FOS-Luc plasmid was a gift from Ron Prywes (Addgene plasmid #11983). ..

    Amplification:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Polymerase Chain Reaction:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
    Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the pGL3-Basic 180 luciferase reporter plasmid (RRID:Addgene_212936). ..

    Clone Assay:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Luciferase:

    Article Title: TFEB coordinates autophagosome biogenesis and ribophagy during starvation via SQSTM1
    Article Snippet: .. The promoter region of human SQSTM1 was amplified by PCR from the U2OS genome and cloned into the pGL3-basic luciferase reporter plasmid (Addgene). ..

    Article Title: tRF-21 LeuTAA Promotes Oxidative Stress by Altering Glutathione Metabolic Enzymes to Support Prostate Cancer Progression
    Article Snippet: Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, and Reactome analyses and visualization were performed using OmicShare ( https://www.omicshare.com ) and SangerBox ( http://www.sangerbox.com/ ). .. The firefly luciferase–expressing plasmids pGL3-basic (RRID:Addgene_212936), pGL3-promoter (RRID:Addgene_172006), and Renilla pRL-CMV (RRID:Addgene_41046) were purchased from Promega (TM033, E2261). ..

    Article Title: Evidence for G-quadruplex-mediated transactivation by the immediate-early 2 protein of human cytomegalovirus
    Article Snippet: .. To produce the UL146 promoter-driven luciferase reporter plasmid (UL146p-Luc), the UL146 promoter region (−927 to +108) was PCR-amplified as an NheI/NcoI fragment and cloned into pGL3-basic (Addgene). ..

    Article Title: METTL3 Methylation Induces Decay of Endogenous Retroelement Transcripts to Promote Tumor Immune Evasion
    Article Snippet: The genes of SMYD1-5, 177 SETD7, SETD8 were inserted into pcDNA3.1(+) vector (RRID:Addgene_52535). .. The 178 SETD1A and E2F4 promoter region, spanning nucleotides −2000 to +100 relative to 179 the transcription start site (TSS), was PCR-amplified and ligated into the pGL3-Basic 180 luciferase reporter plasmid (RRID:Addgene_212936). ..



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    A , Alignment of the human, mouse, and rat Ht sequences is shown. Conserved nucleotides are marked with asterisk. The arrow indicates the TSS. B through E , <t>Luciferase</t> assay in SH‐SY5Y cells transfected for 48 hour with <t>pGL3‐Br</t> ( B and D ) or pGL3‐Ht ( C and E ) and cotransfected with siCTL, siDNMT1, siMeCP2, or siREST ( B , C ), or with EV, DNMT1, MeCP2, or REST ( D and E ). * P ≤0.05 vs siCTL or EV by 1‐way ANOVA analysis followed by Tukey's post hoc test (n=4). CTL indicates control; DNMT1, DNA‐methyltransferase‐1; EV, empty vector; Ht , heart promoter, MeCP2, methyl‐CpG binding protein 2; NCX1, sodium/calcium exchanger 1; pGL3‐Br, Ncx1 brain promoter construct inserted in pGL3basic; pGL3‐Ht, Ncx1 heart promoter construct inserted in pGL3basic; REST, repressor element 1‐silencing transcription factor; si, small interfering; and TSS, transcription start site.
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    A. Quantification of DDAH2 mRNA levels in primary HUVECs naturally carrying the G/G (n = 10) or C/G genotype (n = 5). Total RNA was extracted from confluent cells. cDNA was reverse transcripted and analyzed by quantitative Real-Time PCR technique. Bars represent the means ± SD of two independent experiments carried out in triplicate (* P = 0.008 for G/G HUVECs vs C/G HUVECs); B. Luciferase activity was evaluated in immortalized HUVEC transfected with an empty <t>PGL3</t> luciferase vector (PGL3, light gray bar, n = 21); or a PGL3 vector carrying the rs9267551 G (PGL3G, white bar, n = 21); or a PGL3 vector carrying the rs9267551 C form (PGL3C, black bar, n = 21). Luciferase activity in mock transfected cells (mock, dark gray bar, n = 21) was assessed as a control. (** P = 0.00076 for PGL3G vs. PGL3C).
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    Image Search Results


    A , Alignment of the human, mouse, and rat Ht sequences is shown. Conserved nucleotides are marked with asterisk. The arrow indicates the TSS. B through E , Luciferase assay in SH‐SY5Y cells transfected for 48 hour with pGL3‐Br ( B and D ) or pGL3‐Ht ( C and E ) and cotransfected with siCTL, siDNMT1, siMeCP2, or siREST ( B , C ), or with EV, DNMT1, MeCP2, or REST ( D and E ). * P ≤0.05 vs siCTL or EV by 1‐way ANOVA analysis followed by Tukey's post hoc test (n=4). CTL indicates control; DNMT1, DNA‐methyltransferase‐1; EV, empty vector; Ht , heart promoter, MeCP2, methyl‐CpG binding protein 2; NCX1, sodium/calcium exchanger 1; pGL3‐Br, Ncx1 brain promoter construct inserted in pGL3basic; pGL3‐Ht, Ncx1 heart promoter construct inserted in pGL3basic; REST, repressor element 1‐silencing transcription factor; si, small interfering; and TSS, transcription start site.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Stroke Causes DNA Methylation at Ncx1 Heart Promoter in the Brain Via DNMT1/MeCP2/REST Epigenetic Complex

    doi: 10.1161/JAHA.123.030460

    Figure Lengend Snippet: A , Alignment of the human, mouse, and rat Ht sequences is shown. Conserved nucleotides are marked with asterisk. The arrow indicates the TSS. B through E , Luciferase assay in SH‐SY5Y cells transfected for 48 hour with pGL3‐Br ( B and D ) or pGL3‐Ht ( C and E ) and cotransfected with siCTL, siDNMT1, siMeCP2, or siREST ( B , C ), or with EV, DNMT1, MeCP2, or REST ( D and E ). * P ≤0.05 vs siCTL or EV by 1‐way ANOVA analysis followed by Tukey's post hoc test (n=4). CTL indicates control; DNMT1, DNA‐methyltransferase‐1; EV, empty vector; Ht , heart promoter, MeCP2, methyl‐CpG binding protein 2; NCX1, sodium/calcium exchanger 1; pGL3‐Br, Ncx1 brain promoter construct inserted in pGL3basic; pGL3‐Ht, Ncx1 heart promoter construct inserted in pGL3basic; REST, repressor element 1‐silencing transcription factor; si, small interfering; and TSS, transcription start site.

    Article Snippet: The PCR product was purified using StrataPrep DNA gel extraction kits (Agilent, Milan, Italy) and cloned into multiple cloning sites of pGL3‐basic luciferase expression vector (Promega, Milan, Italy).

    Techniques: Luciferase, Transfection, Plasmid Preparation, Binding Assay, Construct

    A , Top: JASPAR matrix representation (MA0138.1) of the consensus REST binding site on Ht gene ( Ht ‐RE1). Ht ‐RE1 sequence is represented in International Union of Pure and Applied Chemistry code. Bottom: Partial human genomic Ht sequence containing the predicted REST binding site (underlined). B , Luciferase assay in SH‐SY5Y cells under the following experimental conditions: (1) pGL3basic, (2) pGL3‐Ht+EV, (3) pGL3‐Ht+REST, (4) pGL3‐Ht‐RE1mut+EV, (5) pGL3‐Ht‐RE1mut+REST. * P ≤0.05 vs pGL3Ht+EV by 1‐way ANOVA analysis followed by Tukey's post hoc test (n=4). C , ChIP with anti‐REST antibody followed by qPCR of the promoter region containing the RE1 site on the Ht gene in SH‐SY5Y cells transiently transfected with (1) pGL3‐Ht+EV, (2) pGL3‐Ht+REST, (3) pGL3‐Ht+RE1mut+REST. * P ≤0.05 vs pGL3‐Ht+EV by 1‐way ANOVA analysis followed by Tukey's post hoc test (n=4). ChIP indicates chromatin immunoprecipitation; CTL, control; DNMT1, DNA‐methyltransferase‐1; EV, empty vector; Ht , heart promoter, MeCP2, methyl‐CpG binding protein 2; NCX1, sodium/calcium exchanger 1; pGL3‐Br, Ncx1 brain promoter construct inserted in pGL3basic; pGL3‐Ht, Ncx1 heart promoter construct inserted in pGL3basic; qRT‐PCR, quantitative real time polymerase chain reaction; RE1, repressor element 1; and REST, repressor element 1‐silencing transcription factor.

    Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

    Article Title: Stroke Causes DNA Methylation at Ncx1 Heart Promoter in the Brain Via DNMT1/MeCP2/REST Epigenetic Complex

    doi: 10.1161/JAHA.123.030460

    Figure Lengend Snippet: A , Top: JASPAR matrix representation (MA0138.1) of the consensus REST binding site on Ht gene ( Ht ‐RE1). Ht ‐RE1 sequence is represented in International Union of Pure and Applied Chemistry code. Bottom: Partial human genomic Ht sequence containing the predicted REST binding site (underlined). B , Luciferase assay in SH‐SY5Y cells under the following experimental conditions: (1) pGL3basic, (2) pGL3‐Ht+EV, (3) pGL3‐Ht+REST, (4) pGL3‐Ht‐RE1mut+EV, (5) pGL3‐Ht‐RE1mut+REST. * P ≤0.05 vs pGL3Ht+EV by 1‐way ANOVA analysis followed by Tukey's post hoc test (n=4). C , ChIP with anti‐REST antibody followed by qPCR of the promoter region containing the RE1 site on the Ht gene in SH‐SY5Y cells transiently transfected with (1) pGL3‐Ht+EV, (2) pGL3‐Ht+REST, (3) pGL3‐Ht+RE1mut+REST. * P ≤0.05 vs pGL3‐Ht+EV by 1‐way ANOVA analysis followed by Tukey's post hoc test (n=4). ChIP indicates chromatin immunoprecipitation; CTL, control; DNMT1, DNA‐methyltransferase‐1; EV, empty vector; Ht , heart promoter, MeCP2, methyl‐CpG binding protein 2; NCX1, sodium/calcium exchanger 1; pGL3‐Br, Ncx1 brain promoter construct inserted in pGL3basic; pGL3‐Ht, Ncx1 heart promoter construct inserted in pGL3basic; qRT‐PCR, quantitative real time polymerase chain reaction; RE1, repressor element 1; and REST, repressor element 1‐silencing transcription factor.

    Article Snippet: The PCR product was purified using StrataPrep DNA gel extraction kits (Agilent, Milan, Italy) and cloned into multiple cloning sites of pGL3‐basic luciferase expression vector (Promega, Milan, Italy).

    Techniques: Binding Assay, Sequencing, Luciferase, Transfection, Chromatin Immunoprecipitation, Plasmid Preparation, Construct, Quantitative RT-PCR, Real-time Polymerase Chain Reaction

    A. Quantification of DDAH2 mRNA levels in primary HUVECs naturally carrying the G/G (n = 10) or C/G genotype (n = 5). Total RNA was extracted from confluent cells. cDNA was reverse transcripted and analyzed by quantitative Real-Time PCR technique. Bars represent the means ± SD of two independent experiments carried out in triplicate (* P = 0.008 for G/G HUVECs vs C/G HUVECs); B. Luciferase activity was evaluated in immortalized HUVEC transfected with an empty PGL3 luciferase vector (PGL3, light gray bar, n = 21); or a PGL3 vector carrying the rs9267551 G (PGL3G, white bar, n = 21); or a PGL3 vector carrying the rs9267551 C form (PGL3C, black bar, n = 21). Luciferase activity in mock transfected cells (mock, dark gray bar, n = 21) was assessed as a control. (** P = 0.00076 for PGL3G vs. PGL3C).

    Journal: PLoS ONE

    Article Title: A Functional Variant of the Dimethylarginine Dimethylaminohydrolase-2 Gene Is Associated with Insulin Sensitivity

    doi: 10.1371/journal.pone.0036224

    Figure Lengend Snippet: A. Quantification of DDAH2 mRNA levels in primary HUVECs naturally carrying the G/G (n = 10) or C/G genotype (n = 5). Total RNA was extracted from confluent cells. cDNA was reverse transcripted and analyzed by quantitative Real-Time PCR technique. Bars represent the means ± SD of two independent experiments carried out in triplicate (* P = 0.008 for G/G HUVECs vs C/G HUVECs); B. Luciferase activity was evaluated in immortalized HUVEC transfected with an empty PGL3 luciferase vector (PGL3, light gray bar, n = 21); or a PGL3 vector carrying the rs9267551 G (PGL3G, white bar, n = 21); or a PGL3 vector carrying the rs9267551 C form (PGL3C, black bar, n = 21). Luciferase activity in mock transfected cells (mock, dark gray bar, n = 21) was assessed as a control. (** P = 0.00076 for PGL3G vs. PGL3C).

    Article Snippet: Both fragments were inserted in the pGL3 basic luciferase expression vector (Promega, Madison, WI) and constructs sequence was confirmed by direct sequencing.

    Techniques: Real-time Polymerase Chain Reaction, Luciferase, Activity Assay, Transfection, Plasmid Preparation