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rsv pgl3  (Addgene inc)


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    Addgene inc rsv pgl3
    Rsv Pgl3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl-3+basic/RSVpGL3+(RSV+promoter-luciferase+in+pGL3-basic)+(Plasmid+%2340343)/pmc06336686-374-0-5
    Average 92 stars, based on 3 article reviews
    rsv pgl3 - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Luciferase:

    Article Title: The miR-96 and RARγ signaling axis governs androgen signaling and prostate cancer progression
    Article Snippet: .. RSV-pGL3-basic firefly luciferase expressing vector (Addgene) was employed for this purpose, utilizing Fse I and Xba I restriction sites located between the luc + gene and the SV40 pA tail. ..

    Article Title: The miR-96 and RARγ signaling axis governs androgen signaling and prostate cancer progression
    Article Snippet: .. RSV-pGL3-basic firefly luciferase expressing vector (Addgene) was employed for this purpose, utilizing FseI and XbaI restriction sites located between the luc+ gene and the SV40 pA tail. ..

    Article Title: The miR-96 and RARγ signaling axis governs androgen signaling and prostate cancer progression.
    Article Snippet: .. RSV-pGL3-basic firefly luciferase expressing vector (Addgene) was employed for this purpose, utilizing FseI and XbaI restriction sites located between the luc+ gene and the SV40 pA tail. ..

    Article Title: Data-driven computational modeling identifies determinants of glioblastoma response to SHP2 inhibition
    Article Snippet: Flow cytometry Flow cytometry measurements for cell death assays and cell cycle analysis were performed on a BD Biosciences FACSCalibur at the UVA Flow Cytometry Core Facility. .. AP-1 luciferase reporter assay For AP-1 reporter assays, cells were transfected with expression vector 3xAP1pGL3 and RSVpGL3 (Dr. Alexander Dent; Addgene plasmids #40342, 40343). .. Orthotopic tumor xenografts 2,000 G88 cells (parental or expressing control or SHP2 shRNA) were stereotactically injected into the right striatum of 4–8-week-old, female, BALB/c SCID mice (Charles River).

    Expressing:

    Article Title: The miR-96 and RARγ signaling axis governs androgen signaling and prostate cancer progression
    Article Snippet: .. RSV-pGL3-basic firefly luciferase expressing vector (Addgene) was employed for this purpose, utilizing Fse I and Xba I restriction sites located between the luc + gene and the SV40 pA tail. ..

    Article Title: The miR-96 and RARγ signaling axis governs androgen signaling and prostate cancer progression
    Article Snippet: .. RSV-pGL3-basic firefly luciferase expressing vector (Addgene) was employed for this purpose, utilizing FseI and XbaI restriction sites located between the luc+ gene and the SV40 pA tail. ..

    Article Title: The miR-96 and RARγ signaling axis governs androgen signaling and prostate cancer progression.
    Article Snippet: .. RSV-pGL3-basic firefly luciferase expressing vector (Addgene) was employed for this purpose, utilizing FseI and XbaI restriction sites located between the luc+ gene and the SV40 pA tail. ..

    Article Title: Data-driven computational modeling identifies determinants of glioblastoma response to SHP2 inhibition
    Article Snippet: Flow cytometry Flow cytometry measurements for cell death assays and cell cycle analysis were performed on a BD Biosciences FACSCalibur at the UVA Flow Cytometry Core Facility. .. AP-1 luciferase reporter assay For AP-1 reporter assays, cells were transfected with expression vector 3xAP1pGL3 and RSVpGL3 (Dr. Alexander Dent; Addgene plasmids #40342, 40343). .. Orthotopic tumor xenografts 2,000 G88 cells (parental or expressing control or SHP2 shRNA) were stereotactically injected into the right striatum of 4–8-week-old, female, BALB/c SCID mice (Charles River).

    Plasmid Preparation:

    Article Title: The miR-96 and RARγ signaling axis governs androgen signaling and prostate cancer progression
    Article Snippet: .. RSV-pGL3-basic firefly luciferase expressing vector (Addgene) was employed for this purpose, utilizing Fse I and Xba I restriction sites located between the luc + gene and the SV40 pA tail. ..

    Article Title: MicroRNA analysis suggests an additional level of feedback regulation in the NF-κB signaling cascade
    Article Snippet: .. pRSV-luc (RSV promoter-luciferase in pGL3-Basic) plasmid was obtained from Addgene (Plasmid #40343). ..

    Article Title: The miR-96 and RARγ signaling axis governs androgen signaling and prostate cancer progression
    Article Snippet: .. RSV-pGL3-basic firefly luciferase expressing vector (Addgene) was employed for this purpose, utilizing FseI and XbaI restriction sites located between the luc+ gene and the SV40 pA tail. ..

    Article Title: The miR-96 and RARγ signaling axis governs androgen signaling and prostate cancer progression.
    Article Snippet: .. RSV-pGL3-basic firefly luciferase expressing vector (Addgene) was employed for this purpose, utilizing FseI and XbaI restriction sites located between the luc+ gene and the SV40 pA tail. ..

    Article Title: Data-driven computational modeling identifies determinants of glioblastoma response to SHP2 inhibition
    Article Snippet: Flow cytometry Flow cytometry measurements for cell death assays and cell cycle analysis were performed on a BD Biosciences FACSCalibur at the UVA Flow Cytometry Core Facility. .. AP-1 luciferase reporter assay For AP-1 reporter assays, cells were transfected with expression vector 3xAP1pGL3 and RSVpGL3 (Dr. Alexander Dent; Addgene plasmids #40342, 40343). .. Orthotopic tumor xenografts 2,000 G88 cells (parental or expressing control or SHP2 shRNA) were stereotactically injected into the right striatum of 4–8-week-old, female, BALB/c SCID mice (Charles River).

    Reporter Assay:

    Article Title: Data-driven computational modeling identifies determinants of glioblastoma response to SHP2 inhibition
    Article Snippet: Flow cytometry Flow cytometry measurements for cell death assays and cell cycle analysis were performed on a BD Biosciences FACSCalibur at the UVA Flow Cytometry Core Facility. .. AP-1 luciferase reporter assay For AP-1 reporter assays, cells were transfected with expression vector 3xAP1pGL3 and RSVpGL3 (Dr. Alexander Dent; Addgene plasmids #40342, 40343). .. Orthotopic tumor xenografts 2,000 G88 cells (parental or expressing control or SHP2 shRNA) were stereotactically injected into the right striatum of 4–8-week-old, female, BALB/c SCID mice (Charles River).

    Transfection:

    Article Title: Data-driven computational modeling identifies determinants of glioblastoma response to SHP2 inhibition
    Article Snippet: Flow cytometry Flow cytometry measurements for cell death assays and cell cycle analysis were performed on a BD Biosciences FACSCalibur at the UVA Flow Cytometry Core Facility. .. AP-1 luciferase reporter assay For AP-1 reporter assays, cells were transfected with expression vector 3xAP1pGL3 and RSVpGL3 (Dr. Alexander Dent; Addgene plasmids #40342, 40343). .. Orthotopic tumor xenografts 2,000 G88 cells (parental or expressing control or SHP2 shRNA) were stereotactically injected into the right striatum of 4–8-week-old, female, BALB/c SCID mice (Charles River).



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    Image Search Results


    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the pGL 3 -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).

    Journal: PLoS ONE

    Article Title: p21-Activated Kinase 3 (PAK3) Is an AP-1 Regulated Gene Contributing to Actin Organisation and Migration of Transformed Fibroblasts

    doi: 10.1371/journal.pone.0066892

    Figure Lengend Snippet: The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the pGL 3 -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).

    Article Snippet: PCR using high fidelity Expand Plus DNA Polymerase (Roche) was performed on rat genomic DNA, the products were sub-cloned into the pGEM-T Easy vector (Promega) and excised for cloning into the luciferase reported vector, pGL 3 -Basic (Promega) using Mlu1 and Xho1 restriction enzymes.

    Techniques: Binding Assay, Clone Assay, Plasmid Preparation, Luciferase, Transfection, Construct, Activation Assay, Expressing, Inhibition, Activity Assay, Over Expression