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Becton Dickinson perm buffer iii bd 558050
Perm Buffer Iii Bd 558050, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/perm+buffer+iii+bd+558050/pmc11953300-340-9-12
Average 90 stars, based on 1 article reviews
perm buffer iii bd 558050 - by Bioz Stars, 2026-09
90/100 stars

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other:

Article Title: Type III interferon primes pDCs for TLR7 activation and antagonizes immune suppression mediated by TGF-β and PGE2
Article Snippet: This step was followed by a cell permeabilization with Perm Buffer III (BD 558050) for 30 min on ice.

Staining:

Article Title: Circulating Integrin Alpha4/Beta7+ Lymphocytes Targeted by Vedolizumab Have a Pro-Inflammatory Phenotype
Article Snippet: Cells were then stained with a Zombie UV fixable viability dye (BioLegend, Cat 423108), and then incubated with 100 IU/mL Il-2, 2 ng/mL IL-6, 50 pg/mL IL-7, 50 ng/mL IL-21, or no cytokine for 10 minutes, with the exception of IL-21, where cells were incubated for 15 minutes, and then immediately fixed with Fixation Buffer I (BD, Cat 557870) for 15 minutes at 37°C. .. Cells were then washed and permeabilized on ice with Perm Buffer III (BD, Cat 558050) for 30 minutes, washed, and subsequently stained with a panel of antibodies containing both fluorophore-conjugated streptavidin and antibodies to a given phospho-Stat, as well as cell surface markers ( supplementary table 3 ). ..



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