perm buffer (10X Genomics)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

10X Genomics perm buffer
Perm Buffer, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/buffer+perm/pm42106323-210-2-4
Average 86 stars, based on 1 article reviews

perm buffer - by Bioz Stars, 2026-10

86/100 stars

Images

Incubation:

Article Title: Autoimmune neuroinflammation leads to neuronal death via MIF nuclease-mediated parthanatos.
Article Snippet: .. Fix/Perm buffer (10x Genomics, cat. no. PN-2000517), 4% formaldehyde in water) and then incubated overnight at 4 °C. ..

Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development.
Article Snippet: .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Perm Buffer (10x Genomics PN2000517) and 4% formaldehyde, and incubated overnight at 4 ◦C. .. Cells were centrifuged at 850× g for 5 min then the fixation buffer was aspirated off and cells were resuspended and mixed gently with 1 mL of chilled 1× concentration of 8× concentrated quenching buffer (10x Genomics PN-2000516).

Article Title: Autoimmune neuroinflammation leads to neuronal death via MIF nuclease-mediated parthanatos
Article Snippet: .. Fix/Perm buffer (10x Genomics, cat. no. PN-2000517), 4% formaldehyde in water) and then incubated overnight at 4 °C. ..

Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development
Article Snippet: .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Perm Buffer (10x Genomics PN-2000517) and 4% formaldehyde, and incubated overnight at 4 °C. .. Cells were centrifuged at 850× g for 5 min then the fixation buffer was aspirated off and cells were resuspended and mixed gently with 1 mL of chilled 1× concentration of 8× concentrated quenching buffer (10x Genomics PN-2000516).

Staining:

Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development
Article Snippet: .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Perm Buffer (10x Genomics PN-2000517) and 4% formaldehyde, and incubated overnight at 4 °C. .. Cells were centrifuged at 850× g for 5 min then the fixation buffer was aspirated off and cells were resuspended and mixed gently with 1 mL of chilled 1× concentration of 8× concentrated quenching buffer (10x Genomics PN-2000516).

Concentration Assay:

Article Title: Transcriptomic Plasticity of Human Alveolar Macrophages Revealed by Single-Cell RNA Sequencing Following Drug Exposure: Implications for Therapeutic Development
Article Snippet: .. Live cells were counted in a haemocytometer using trypan blue stain, then centrifuged and resuspended in 1 mL of freshly prepared fixation buffer, containing 1× concentration of 10× concentrated Fix & Perm Buffer (10x Genomics PN-2000517) and 4% formaldehyde, and incubated overnight at 4 °C. .. Cells were centrifuged at 850× g for 5 min then the fixation buffer was aspirated off and cells were resuspended and mixed gently with 1 mL of chilled 1× concentration of 8× concentrated quenching buffer (10x Genomics PN-2000516).

Hybridization:

Article Title: Catecholaminergic neurons boost fibroblast osteogenic activity in keloid.
Article Snippet: .. Fix & Perm Buffer (10X Genomics cat. PN-2000517) at 4°C for 20 h. Afterwards, the fixation was quenched, and the cells were stored at −80°C until probe hybridization. .. Fix & Perm Buffer (10X Genomics cat. PN-2000517) at 4°C for 20 h. Afterwards, the fixation was quenched, and the cells were stored at −80°C until probe hybridization.

Review