perm buffer (fluidigm)
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perm buffer
Perm Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/Maxpar+Perm-S+Buffer/pmc10915970-579-12-23
Average 93 stars, based on 68 article reviews
Perm Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/Maxpar+Perm-S+Buffer/pmc10915970-579-12-23
Average 93 stars, based on 68 article reviews
perm buffer - by Bioz Stars,
2026-10
93/100 stars
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Staining:Article Title: The use of mass cytometry (CyTOF) to evaluate the cellular uptake of stable radiopharmaceutical surrogates in single cells: a proof-of-concept study Article Snippet: .. For DNA staining, required for event identification and singlet gating, cells were incubated with Cell-IDTM Intercalator-Ir (125 nM, Standard BioTools), prepared in Maxpar ® Fix and Article Title: NKG2A+ NK Cell Cytotoxicity of Epstein-Barr Virus Infected B Cells is Mediated Through the NKG2D and NKp30 Activating Receptors Article Snippet: Following surface staining, cells were washed in CSB and fixed with eBioscience fix/perm buffer (eBioscience) for 30 minutes at RT. .. Cells were then washed and stained with intracellular antibodies in Article Title: Reducing bias in mass cytometry data through corrections for spillover and nonspecific binding Article Snippet: For regular staining, cells were incubated with antibodies at concentrations of 4 μg/mL in PBS-S for 30 minutes on a thermomixer (Eppendorf) at 37°C, 1,100 rpm. .. Cells were then centrifuged, washed with PBS-S, followed by cell staining buffer, and then incubated overnight at 4°C with 62.5 nM of Cell-ID Intercalator-Ir in Maxpar Fix and Article Title: Phase II Study of Pembrolizumab Plus Capecitabine and Bevacizumab for Microsatellite Stable/Mismatch Repair-Proficient Metastatic Colorectal Cancer. Article Snippet: This study found that combination of pembrolizumab with capecitabine and bevacizumab has an expected adverse event profile in patients with microsatellite stable metastatic colorectal cancer.. The overall response rate of 5% did not meet the prespecified target for the primary efficacy endpoint, however median duration of response and progression-free survival > 6 months suggest clinical benefit in a subset of patients.. Background: This study evaluated the safet y, tolerabilit y and preliminary efficacy of pembrolizumab in combination with capecitabine and bevacizumab in microsatellite stable (MSS) metastatic colorectal cancer (mCRC). Incubation:Article Title: The use of mass cytometry (CyTOF) to evaluate the cellular uptake of stable radiopharmaceutical surrogates in single cells: a proof-of-concept study Article Snippet: .. For DNA staining, required for event identification and singlet gating, cells were incubated with Cell-IDTM Intercalator-Ir (125 nM, Standard BioTools), prepared in Maxpar ® Fix and Article Title: Type I interferon signaling promotes mucosal inflammation in murine models of colitis Article Snippet: For mass cytometry, cells were first incubated at room temperature with Cell-ID Cisplatin (Standard BioTools) for 20 minutes and then with TruStain FcX (BioLegend) for 10 minutes. .. Then, cells were incubated with metal-conjugated primary antibodies that target surface proteins (Supplemental Table 4) at room temperature for 30 minutes, followed by fixation and permeabilization using Maxpar Fix I buffer for 20 minutes and Article Title: Reducing bias in mass cytometry data through corrections for spillover and nonspecific binding Article Snippet: For regular staining, cells were incubated with antibodies at concentrations of 4 μg/mL in PBS-S for 30 minutes on a thermomixer (Eppendorf) at 37°C, 1,100 rpm. .. Cells were then centrifuged, washed with PBS-S, followed by cell staining buffer, and then incubated overnight at 4°C with 62.5 nM of Cell-ID Intercalator-Ir in Maxpar Fix and Article Title: Single-cell analysis of signalling and transcriptional responses to type I interferons. Article Snippet: Cells were resuspended in 1.6% (v/v) formaldehyde (Pierce, #28906) diluted in Maxpar PBS and incubated for 10min at room temperature. .. Cells were resuspended in 125mM cell-ID Intercalator (Fluidigm) diluted in Maxpar Fix and Article Title: Latent Neoehrlichia mikurensis Infections May Be Reactivated in Patients With B-Cell Lymphomas Treated With Rituximab. Article Snippet: The samples were washed, fixed with 1.6% formaldehyde and permeabilised using Foxp3/ Transcription Factor Staining buffer (eBioscience, San Diego, CA, USA) for 60 min at RT prior to a 60 min- incubation at RT with antibodies directed against five intracellular markers (Table S3). .. The cells were then washed, incubated for 45 min at RT with 62.5 nM Cell- ID Intercalator- Ir diluted in Maxpar Fix and Gentle:Article Title: Reducing bias in mass cytometry data through corrections for spillover and nonspecific binding Article Snippet: For regular staining, cells were incubated with antibodies at concentrations of 4 μg/mL in PBS-S for 30 minutes on a thermomixer (Eppendorf) at 37°C, 1,100 rpm. .. Cells were then centrifuged, washed with PBS-S, followed by cell staining buffer, and then incubated overnight at 4°C with 62.5 nM of Cell-ID Intercalator-Ir in Maxpar Fix and Blocking Assay:Article Title: Superior Intracellular Detection of Cytokines, Transcription Factors, and Phosphoproteins by CyTOF Compared With Fluorescent Cytometry. Article Snippet: .. After washing with CSB, cells were fixed with Maxpar Fix I Buffer (Standard BioTools), permeabilized with |