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Becton Dickinson bd cytofix/cytoperm buffer
Bd Cytofix/Cytoperm Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/bd+perm+wash+buffer/pmc06032104-221-8-10
Average 90 stars, based on 1 article reviews
bd cytofix/cytoperm buffer - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Incubation:

Article Title: Germline Single-Nucleotide Polymorphism GFI1-36N Causes Alterations in Mitochondrial Metabolism and Leads to Increased ROS-Mediated DNA Damage in a Murine Model of Human Acute Myeloid Leukemia.
Article Snippet: Cells were centrifuged with FACS buffer (395× g, 4 min, 4 ◦C), the supernatant was removed and 100 μL BD Cytofix/Cytoperm buffer (BD, Franklin Lakes, NJ, USA) was added.

Article Title: RBD-depleted SARS-CoV-2 spike generates protective immunity in cynomolgus macaques
Article Snippet: After 30 min of incubation at 4 °C, in the dark, cells were washed in BD Perm/Wash buffer and then acquired on the LSRII cytometer (Beckton Dickinson).

Article Title: The Simultaneous Deletion of pH-Sensing Receptors GPR4 and OGR1 (GPR68) Ameliorates Colitis with Additive Effects on Multiple Parameters of Inflammation
Article Snippet: For fixation, BD Cytofix/Cytoperm (554722, Becton Dickinson, Franklin Lakes, NJ, USA) and BD Perm/Wash (554723, Becton Dickinson, Franklin Lakes, NJ, USA ) were used following the manufacturer’s instructions.

Article Title: Modulation of bone marrow haematopoietic stem cell activity as a therapeutic strategy after myocardial infarction: a preclinical study
Article Snippet: Subsequently, cells were fixed for 10 min at 4 °C using BD Cytofix/Cytoperm Buffer (Becton Dickinson and Company) and intracellular Ki-67-PE (Invitrogen, cat. no. 12-5698-80, 1:500) staining was performed using PermWash solution for at least 45 min at 4 °C (Becton Dickinson and Company).

Article Title: Uncovering Functional Gene Regulatory Networks in Bulk and Single-Cell Data through Robust Transcription Factor Activity Estimation and Model-Guided Experimental Validation
Article Snippet: After an additional wash, intracellular staining was performed using the BD Perm/Wash buffer containing anti-mouse Nanog-eFluor 660 antibodies for 1 hour at room temperature.

Article Title: Diacylglycerol kinase ζ dictates CD40-mediated immune synapse formation, mTORC1 signaling and plasma cell fate in B lymphocytes
Article Snippet: After washing with permeabilization buffer (BD Perm/Wash Buffer), cells were resuspended in the antibody mix prepared using the permeabilization buffer (30 min incubation, 4°C).

Article Title: Establishment of reproducible method for production of cultured epidermal sheets with controlled melanocyte density
Article Snippet: Cells were immersed in Fixation/Permeabilization solution (Becton Dickinson, US) for 20 min at 4 °C and washed with 1× BD Perm/Wash buffer prepared by diluting BD Perm/Wash (Becton Dickinson) with MilliQ water.

Cytometry:

Article Title: Germline Single-Nucleotide Polymorphism GFI1-36N Causes Alterations in Mitochondrial Metabolism and Leads to Increased ROS-Mediated DNA Damage in a Murine Model of Human Acute Myeloid Leukemia.
Article Snippet: Cells were centrifuged with FACS buffer (395× g, 4 min, 4 ◦C), the supernatant was removed and 100 μL BD Cytofix/Cytoperm buffer (BD, Franklin Lakes, NJ, USA) was added.

Article Title: RBD-depleted SARS-CoV-2 spike generates protective immunity in cynomolgus macaques
Article Snippet: After 30 min of incubation at 4 °C, in the dark, cells were washed in BD Perm/Wash buffer and then acquired on the LSRII cytometer (Beckton Dickinson).

Article Title: The Simultaneous Deletion of pH-Sensing Receptors GPR4 and OGR1 (GPR68) Ameliorates Colitis with Additive Effects on Multiple Parameters of Inflammation
Article Snippet: For fixation, BD Cytofix/Cytoperm (554722, Becton Dickinson, Franklin Lakes, NJ, USA) and BD Perm/Wash (554723, Becton Dickinson, Franklin Lakes, NJ, USA ) were used following the manufacturer’s instructions.

Article Title: Modulation of bone marrow haematopoietic stem cell activity as a therapeutic strategy after myocardial infarction: a preclinical study
Article Snippet: Subsequently, cells were fixed for 10 min at 4 °C using BD Cytofix/Cytoperm Buffer (Becton Dickinson and Company) and intracellular Ki-67-PE (Invitrogen, cat. no. 12-5698-80, 1:500) staining was performed using PermWash solution for at least 45 min at 4 °C (Becton Dickinson and Company).

Article Title: Uncovering Functional Gene Regulatory Networks in Bulk and Single-Cell Data through Robust Transcription Factor Activity Estimation and Model-Guided Experimental Validation
Article Snippet: After an additional wash, intracellular staining was performed using the BD Perm/Wash buffer containing anti-mouse Nanog-eFluor 660 antibodies for 1 hour at room temperature.

Article Title: Diacylglycerol kinase ζ dictates CD40-mediated immune synapse formation, mTORC1 signaling and plasma cell fate in B lymphocytes
Article Snippet: After washing with permeabilization buffer (BD Perm/Wash Buffer), cells were resuspended in the antibody mix prepared using the permeabilization buffer (30 min incubation, 4°C).

Article Title: Establishment of reproducible method for production of cultured epidermal sheets with controlled melanocyte density
Article Snippet: Cells were immersed in Fixation/Permeabilization solution (Becton Dickinson, US) for 20 min at 4 °C and washed with 1× BD Perm/Wash buffer prepared by diluting BD Perm/Wash (Becton Dickinson) with MilliQ water.



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