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Becton Dickinson phosflow perm buffer
Phosflow Perm Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perm+buffer/phosflow+perm+buffer+iii/pm28930662-251-20-25
Average 90 stars, based on 1 article reviews
phosflow perm buffer - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Staining:

Article Title: Monovalent glycoconjugates of sulforaphane prevent inflammation induced by lipopolysaccharide in human dendritic cells by inhibiting NF-ĸB signalling pathway.
Article Snippet: .. Monocyte-derived DCs (MoDCs; 7.5 105) were grown in 96-well plates at 37 C and 5% CO2 for 10 min. For effective intracellular staining of p65 NF-κB and MAPKs, the moDCs were fixed with 1% paraformaldehyde and permeabilized using BD Phosflow Perm Buffer III (Becton, Dickinson, [BD], Franklin Lakes, NJ). .. Specific monoclonal antibodies (moAbs), detected using different lasers, were used to identify changes in the different signalling pathways: MAPK (pT180/pY182) (BD Biosciences Cat# 560313, RRID:AB_1645295), p65 NF-KB (BD Biosciences Cat# 558423, RRID:AB_647222) and JNK (BD Biosciences Cat# 562481, RRID:AB_11153116) (Table S1).

Article Title: Pro-Apoptotic Activity of 1-(4,5,6,7-Tetrabromo-1 H -benzimidazol-1-yl)propan-2-one, an Intracellular Inhibitor of PIM-1 Kinase in Acute Lymphoblastic Leukemia and Breast Cancer Cells.
Article Snippet: .. PhosFlow Analysis CCRF-CEM cells were prepared following the BD PhosFlowTM Protocol for Human PBMCs using NF-κB p65 (pS529)-PE (#558423), BD PhosFlowTM Perm Buffer III (#558050), Cytofix Fixation Buffer (#554655), and Stain Buffer FBS (#554656), all obtained from Becton Dickinson. .. Flow cytometric analysis was performed using a BD Accuri C6 Plus cytometer (Becton Dickinson) according to the manufacturer’s instructions.

Article Title: Post-transcriptional regulation by TIA1 and TIAL1 controls the transcriptional program enforcing T cell quiescence
Article Snippet: .. After 30 min. incubation at 37°C, cells were directly fix and permeabilised by adding 1/5 BD™ Phosflow Fix Buffer, 15 min at RT, followed by cell wash, permeabilization with BD™ Phosflow Perm Buffer III, O/N at 4°C and protein staining as described above. .. Flow cytometry data were collected using a BD Fortessa cytometer and analysed using FlowJo v10.

Incubation:

Article Title: Synergistic Effects of PARP Inhibition and Cholesterol Biosynthesis Pathway Modulation.
Article Snippet: The cell pellet was resuspended in 300 μL of cold EasySep buffer (StemCell, #20144). .. Cells were permeabilized by slowly adding 500 μL cold BD Phosflow Perm Buffer III (BD #558050) followed by 30 minutes of incubation at �20°C. .. Cells were centrifuged 400 g for 5 minutes, washed twice with 750 μL of EasySep buffer, and resuspended in 50 μL of master mix containing CD3 APC-R700 1:200 [BD Biosciences Cat# 565119 (also 565120), RRID:AB_2744385], CD4-BUV395 1: 100 (BD Biosciences Cat# 564724, RRID:AB_2738917), CD8-BB515 1:200 [BD Biosciences Cat# 564526 (also 564527), RRID:AB_2744458], CD14-BV711 1: 400 [BD Biosciences Cat# 563372 (also 563373), RRID:AB_2744290], CD15BV605 1:800 [BD Biosciences Cat# 562979 (also 562980), RRID:AB_2744292], CD19-BV480 1:200 (BD Biosciences Cat# 566103, RRID:AB_2739505), CD16BV421 1:1,000 (BD Biosciences Cat# 562878, RRID:AB_2737861), CD45-PECy5 1:200 (BD Biosciences Cat# 560974, RRID:AB_10562039), CD56-PECF594 1:800 (BD Biosciences Cat# 564849, RRID:AB_2738983), pSTAT5-PE 1:50 (BD Biosciences Cat# 562077, RRID:AB_10894188), HLA-DR-BV786 1: 200 (BD Biosciences Cat# 564041, RRID:AB_2738559), and CD11c-APC 1:400 (BD Biosciences Cat# 559877, RRID:AB_398680) in Brilliant Stain Buffer (BD #563716).

Article Title: Post-transcriptional regulation by TIA1 and TIAL1 controls the transcriptional program enforcing T cell quiescence
Article Snippet: .. After 30 min. incubation at 37°C, cells were directly fix and permeabilised by adding 1/5 BD™ Phosflow Fix Buffer, 15 min at RT, followed by cell wash, permeabilization with BD™ Phosflow Perm Buffer III, O/N at 4°C and protein staining as described above. .. Flow cytometry data were collected using a BD Fortessa cytometer and analysed using FlowJo v10.



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