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pentr psuper expression vector  (Addgene inc)


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    Addgene inc pentr psuper expression vector
    Pentr Psuper Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pentr+expression+vector/pSUPER+empty+vector+(Plasmid+%2326212)/pmc08120935-41-41-45
    Average 93 stars, based on 7 article reviews
    pentr psuper expression vector - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Expressing:

    Article Title: Synaptic localisation of SRF coactivators, MKL1 and MKL2, and their role in dendritic spine morphology
    Article Snippet: .. For spine density and morphological analyses, in addition to vectors expressing sh MKL1 and sh MKL2 , pSynapsinGFP with pSUPER (empty vector), pTRIP-shLuc (shLuc; where shLuc is a shRNA targeting luciferase), or pLKO-shMKL1/2 (sh MKL1 / 2 ), which has a different sequence, were used. pLKO.1-MKL1/2 shRNA was a gift from Ron Prywes (Addgene plasmid # 27161) . pLKO.1-MKL1/2 shRNA shows 100% similarity to rat MKL2 and 95% similarity to rat MKL1. ..

    Article Title: CD44 regulates dendrite morphogenesis through Src tyrosine kinase-dependent positioning of the Golgi.
    Article Snippet: Electroporation was performed in a Nepagene WY21 apparatus at 4 minutes after the injection with two trains of six pulses (80 V, 50-ms duration) delivered through 5-mm electrode paddles that were placed on each side of the skull. .. The following mammalian expression plasmids have been described previously: pSuper vector (Brummelkamp et al., 2002), b-actin-GFP (Jaworski et al., 2005), b-actin-RFP (Hoogenraad et al., 2007), EFa-b-gal Jo ur na l o f C el l S ci en ce (Konopka et al., 2005) and pLNCX chick src Y527F (constitutively active Src; Addgene plasmid 13660; http://www.addgene.org/13660/). ..

    Article Title: Krüppel-like factor 7 deficiency disrupts corpus callosum development and neuronal migration in the developing mouse cerebral cortex.
    Article Snippet: .. To generate the expression of short hairpin RNAs (shRNAs), two pairs of synthetic oligonucleotides were individually cloned into the pSuper vector (Addgene), in which the shRNAs are under the control of a human H1 promoter [18]. ..

    Article Title: Regulation of GC box activity by 8-oxoguanine
    Article Snippet: .. The HeLa-derived cell line, in which the expression of OGG1 DNA glycosylase was stably knocked down by expression of the specific shRNA (OGG1-sh), was described previously along with the corresponding congenic OGG1-proficient HeLa-pEpS cell line (Ctrl-sh) stably transfected with the empty pENTR/pSuper + expression vector (Addgene, Cambridge, MA) [ ]. ..

    Article Title: Krüppel‐like factor 7 deficiency disrupts corpus callosum development and neuronal migration in the developing mouse cerebral cortex
    Article Snippet: .. To generate the expression of short hairpin RNAs (shRNAs), two pairs of synthetic oligonucleotides were individually cloned into the pSuper vector (Addgene), in which the shRNAs are under the control of a human H1 promoter [ ]. ..

    shRNA:

    Article Title: Synaptic localisation of SRF coactivators, MKL1 and MKL2, and their role in dendritic spine morphology
    Article Snippet: .. For spine density and morphological analyses, in addition to vectors expressing sh MKL1 and sh MKL2 , pSynapsinGFP with pSUPER (empty vector), pTRIP-shLuc (shLuc; where shLuc is a shRNA targeting luciferase), or pLKO-shMKL1/2 (sh MKL1 / 2 ), which has a different sequence, were used. pLKO.1-MKL1/2 shRNA was a gift from Ron Prywes (Addgene plasmid # 27161) . pLKO.1-MKL1/2 shRNA shows 100% similarity to rat MKL2 and 95% similarity to rat MKL1. ..

    Article Title: Short-Term Mitochondrial Permeability Transition Pore Opening Modulates Histone Lysine Methylation at the Early Phase of Somatic Cell Reprogramming.
    Article Snippet: pMXs-Oct4, Sox2, Klf4 and c-Myc were purchased from Addgene. pWP-TetOn vectors were constructed by replacing the EF-1a promoter of the pWPXLd vector with the TRE promoter of pTRE-Tight vector (Clontech) described before (Chen et al., 2011). .. ShPHF8, ShCypD and ShNrf2 vector was made by inserting PHF8, CypD and Nrf2 shRNA into pSUPER vector. pMXs-CypD, iCypD-Dox and iPHF8-Dox vectors were made by inserting CypD or PHF8 cDNAs obtained from mouse fibroblasts into pMXsFlag (Addgene) and pWP-TetOn vectors, respectively. ..

    Article Title: Regulation of GC box activity by 8-oxoguanine
    Article Snippet: .. The HeLa-derived cell line, in which the expression of OGG1 DNA glycosylase was stably knocked down by expression of the specific shRNA (OGG1-sh), was described previously along with the corresponding congenic OGG1-proficient HeLa-pEpS cell line (Ctrl-sh) stably transfected with the empty pENTR/pSuper + expression vector (Addgene, Cambridge, MA) [ ]. ..

    Luciferase:

    Article Title: Synaptic localisation of SRF coactivators, MKL1 and MKL2, and their role in dendritic spine morphology
    Article Snippet: .. For spine density and morphological analyses, in addition to vectors expressing sh MKL1 and sh MKL2 , pSynapsinGFP with pSUPER (empty vector), pTRIP-shLuc (shLuc; where shLuc is a shRNA targeting luciferase), or pLKO-shMKL1/2 (sh MKL1 / 2 ), which has a different sequence, were used. pLKO.1-MKL1/2 shRNA was a gift from Ron Prywes (Addgene plasmid # 27161) . pLKO.1-MKL1/2 shRNA shows 100% similarity to rat MKL2 and 95% similarity to rat MKL1. ..

    Sequencing:

    Article Title: Synaptic localisation of SRF coactivators, MKL1 and MKL2, and their role in dendritic spine morphology
    Article Snippet: .. For spine density and morphological analyses, in addition to vectors expressing sh MKL1 and sh MKL2 , pSynapsinGFP with pSUPER (empty vector), pTRIP-shLuc (shLuc; where shLuc is a shRNA targeting luciferase), or pLKO-shMKL1/2 (sh MKL1 / 2 ), which has a different sequence, were used. pLKO.1-MKL1/2 shRNA was a gift from Ron Prywes (Addgene plasmid # 27161) . pLKO.1-MKL1/2 shRNA shows 100% similarity to rat MKL2 and 95% similarity to rat MKL1. ..

    Plasmid Preparation:

    Article Title: Synaptic localisation of SRF coactivators, MKL1 and MKL2, and their role in dendritic spine morphology
    Article Snippet: .. For spine density and morphological analyses, in addition to vectors expressing sh MKL1 and sh MKL2 , pSynapsinGFP with pSUPER (empty vector), pTRIP-shLuc (shLuc; where shLuc is a shRNA targeting luciferase), or pLKO-shMKL1/2 (sh MKL1 / 2 ), which has a different sequence, were used. pLKO.1-MKL1/2 shRNA was a gift from Ron Prywes (Addgene plasmid # 27161) . pLKO.1-MKL1/2 shRNA shows 100% similarity to rat MKL2 and 95% similarity to rat MKL1. ..

    Article Title: CD44 regulates dendrite morphogenesis through Src tyrosine kinase-dependent positioning of the Golgi.
    Article Snippet: Electroporation was performed in a Nepagene WY21 apparatus at 4 minutes after the injection with two trains of six pulses (80 V, 50-ms duration) delivered through 5-mm electrode paddles that were placed on each side of the skull. .. The following mammalian expression plasmids have been described previously: pSuper vector (Brummelkamp et al., 2002), b-actin-GFP (Jaworski et al., 2005), b-actin-RFP (Hoogenraad et al., 2007), EFa-b-gal Jo ur na l o f C el l S ci en ce (Konopka et al., 2005) and pLNCX chick src Y527F (constitutively active Src; Addgene plasmid 13660; http://www.addgene.org/13660/). ..

    Article Title: Short-Term Mitochondrial Permeability Transition Pore Opening Modulates Histone Lysine Methylation at the Early Phase of Somatic Cell Reprogramming.
    Article Snippet: pMXs-Oct4, Sox2, Klf4 and c-Myc were purchased from Addgene. pWP-TetOn vectors were constructed by replacing the EF-1a promoter of the pWPXLd vector with the TRE promoter of pTRE-Tight vector (Clontech) described before (Chen et al., 2011). .. ShPHF8, ShCypD and ShNrf2 vector was made by inserting PHF8, CypD and Nrf2 shRNA into pSUPER vector. pMXs-CypD, iCypD-Dox and iPHF8-Dox vectors were made by inserting CypD or PHF8 cDNAs obtained from mouse fibroblasts into pMXsFlag (Addgene) and pWP-TetOn vectors, respectively. ..

    Article Title: Krüppel-like factor 7 deficiency disrupts corpus callosum development and neuronal migration in the developing mouse cerebral cortex.
    Article Snippet: .. To generate the expression of short hairpin RNAs (shRNAs), two pairs of synthetic oligonucleotides were individually cloned into the pSuper vector (Addgene), in which the shRNAs are under the control of a human H1 promoter [18]. ..

    Article Title: VAP‐SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function
    Article Snippet: .. The following plasmids are described previously: Myc‐VAPA, Myc‐VAPB, HA‐VAPA (with E178G mutation), HA‐VAPB, HA‐VAPB‐K87D/M89D, GFP‐VAPB‐TM, GFP‐VAPB‐MSP‐CC, and VAPB‐MSP‐GFP (with E6I single nucleotide polymorphism and K139R mutation; Teuling et al , ); pGFPC1‐Sec61β (Hradsky et al , ); HA‐BirA (de Boer et al , ); BioGFP (Jaworski et al , ); GW1‐RFP and pGW1‐GFP (Hoogenraad et al , ); pSuper vector (Brummelkamp et al , ); ssRFP‐KDEL (Addgene plasmid #62236, gift from Dr. Erik Snapp; Snapp et al , ), TagRFP‐ER (Schatzle et al , ), HA‐Erlin1, and HA‐Erlin2 (gift from Dr. Richard J.H. .. Wojcikiewicz; Pearce et al , , ); GCaMP6f (Addgene plasmid #58514, gift from Prof. Adam E. Cohen; Venkatachalam & Cohen, ); and R‐GECO1 (Addgene plasmid #45494, gift from Prof. Robert E. Campbell; Wu et al , ).

    Article Title: Krüppel‐like factor 7 deficiency disrupts corpus callosum development and neuronal migration in the developing mouse cerebral cortex
    Article Snippet: .. To generate the expression of short hairpin RNAs (shRNAs), two pairs of synthetic oligonucleotides were individually cloned into the pSuper vector (Addgene), in which the shRNAs are under the control of a human H1 promoter [ ]. ..

    Clone Assay:

    Article Title: Krüppel-like factor 7 deficiency disrupts corpus callosum development and neuronal migration in the developing mouse cerebral cortex.
    Article Snippet: .. To generate the expression of short hairpin RNAs (shRNAs), two pairs of synthetic oligonucleotides were individually cloned into the pSuper vector (Addgene), in which the shRNAs are under the control of a human H1 promoter [18]. ..

    Article Title: Krüppel‐like factor 7 deficiency disrupts corpus callosum development and neuronal migration in the developing mouse cerebral cortex
    Article Snippet: .. To generate the expression of short hairpin RNAs (shRNAs), two pairs of synthetic oligonucleotides were individually cloned into the pSuper vector (Addgene), in which the shRNAs are under the control of a human H1 promoter [ ]. ..

    Control:

    Article Title: Krüppel-like factor 7 deficiency disrupts corpus callosum development and neuronal migration in the developing mouse cerebral cortex.
    Article Snippet: .. To generate the expression of short hairpin RNAs (shRNAs), two pairs of synthetic oligonucleotides were individually cloned into the pSuper vector (Addgene), in which the shRNAs are under the control of a human H1 promoter [18]. ..

    Article Title: Krüppel‐like factor 7 deficiency disrupts corpus callosum development and neuronal migration in the developing mouse cerebral cortex
    Article Snippet: .. To generate the expression of short hairpin RNAs (shRNAs), two pairs of synthetic oligonucleotides were individually cloned into the pSuper vector (Addgene), in which the shRNAs are under the control of a human H1 promoter [ ]. ..

    other:

    Article Title: Synaptic control of DNA methylation involves activity-dependent degradation of DNMT3A1 in the nucleus
    Article Snippet: The shRNA constructs to knock down human DNMT3A1/3A2 and scrambled controls were hDNMT3A shRNA, pSMP-DNMT3A1 and pSMP-Luc (a gift from George Daley, Addgene plasmid #36380, Addgene plasmid #36394, respectively).

    Stable Transfection:

    Article Title: Regulation of GC box activity by 8-oxoguanine
    Article Snippet: .. The HeLa-derived cell line, in which the expression of OGG1 DNA glycosylase was stably knocked down by expression of the specific shRNA (OGG1-sh), was described previously along with the corresponding congenic OGG1-proficient HeLa-pEpS cell line (Ctrl-sh) stably transfected with the empty pENTR/pSuper + expression vector (Addgene, Cambridge, MA) [ ]. ..

    Transfection:

    Article Title: Regulation of GC box activity by 8-oxoguanine
    Article Snippet: .. The HeLa-derived cell line, in which the expression of OGG1 DNA glycosylase was stably knocked down by expression of the specific shRNA (OGG1-sh), was described previously along with the corresponding congenic OGG1-proficient HeLa-pEpS cell line (Ctrl-sh) stably transfected with the empty pENTR/pSuper + expression vector (Addgene, Cambridge, MA) [ ]. ..

    Mutagenesis:

    Article Title: VAP‐SCRN1 interaction regulates dynamic endoplasmic reticulum remodeling and presynaptic function
    Article Snippet: .. The following plasmids are described previously: Myc‐VAPA, Myc‐VAPB, HA‐VAPA (with E178G mutation), HA‐VAPB, HA‐VAPB‐K87D/M89D, GFP‐VAPB‐TM, GFP‐VAPB‐MSP‐CC, and VAPB‐MSP‐GFP (with E6I single nucleotide polymorphism and K139R mutation; Teuling et al , ); pGFPC1‐Sec61β (Hradsky et al , ); HA‐BirA (de Boer et al , ); BioGFP (Jaworski et al , ); GW1‐RFP and pGW1‐GFP (Hoogenraad et al , ); pSuper vector (Brummelkamp et al , ); ssRFP‐KDEL (Addgene plasmid #62236, gift from Dr. Erik Snapp; Snapp et al , ), TagRFP‐ER (Schatzle et al , ), HA‐Erlin1, and HA‐Erlin2 (gift from Dr. Richard J.H. .. Wojcikiewicz; Pearce et al , , ); GCaMP6f (Addgene plasmid #58514, gift from Prof. Adam E. Cohen; Venkatachalam & Cohen, ); and R‐GECO1 (Addgene plasmid #45494, gift from Prof. Robert E. Campbell; Wu et al , ).



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    Image Search Results


    (a) Western blot analysis of doxycycline (dox)-inducible CTU2 knockdown in HeLa cells over time. Duration of dox treatment is indicated in days. scrambled, non-targeting shRNA control. (b) APM-northern blot validation of tRNA thiolation loss in HeLa and RPE1 cells after 4 days of dox treatment. is shown representatively. (c) Viability analysis of CTU2 knockdown cell lines by Annexin V staining. +CTU2 res indicates shRNA-resistant CTU2 overexpression. One-way ANOVA, WT vs. CTU2 res p = 0.264. n = 3 technical replicates. (d) Western blot analysis of unfolded protein response (UPR) checkpoints during progressive tRNA hypothiolation in RPE1 cells. Time points indicate days of CTU2 depletion. (e) Western blot validation of selected proteins identified in DREAM-PL patient fibroblasts, analyzed under acute CTU2 knockdown conditions. (f) Ribosome profiling in 96 h CTU2 -depleted RPE1 cells compared with untreated controls. Normalized codon occupancy is shown for A- and G-ending codons of the indicated amino acids. Codons are divided into quartiles according to relative library abundance. n = 2 technical replicates. (g) Cumulative A-ending codon frequency (AAA, CAA, GAA, and AGA) in coding sequences (CDSs) of proteins quantified in CTU2 -depleted RPE1 cells after 96 hours. The dashed line indicates the average A-ending codon content (7.6%) across 19,085 analyzed human CDSs. Two-tailed unpaired t-test, p > 0.001 (down and up). (h) Gene Ontology (GO) enrichment analysis on the top 5% of human coding sequences ranked by A-ending codon content (AAA, CAA, GAA, AGA). Enriched pathways were clustered by GO Biological Process (2023). Cilium-associated terms are highlighted in orange. (i) Ribosome occupancy analysis of all mRNAs measured by ribosome profiling (f). Transcripts were ranked by A-ending codon frequency (AAA, CAA, GAA and AGA) and divided into ten equal bins (bin 1 = lowest, bin 10 = highest). A negative correlation was observed between A-ending codon frequency and ribosome abundance. ANOVA with post hoc test. Adjusted p-values are indicated. (j) Ciliogenesis assay in CTU2 -knockdown and wildtype RPE1 cells upon serum starvation. Representative immunofluorescence images show primary cilia (green) and nuclei (blue). The treatment scheme is shown above. Quantification of fractions of cilia-forming RPE1 cells under tRNA hypothiolation is provided. Two-tailed unpaired t-test, p-values are indicated. n = 3 technical replicates. Scale bars 10µm.

    Journal: bioRxiv

    Article Title: tRNA thiolation defects disrupt cellular proteostasis and tissue homeostasis in mammals

    doi: 10.1101/2025.10.24.684405

    Figure Lengend Snippet: (a) Western blot analysis of doxycycline (dox)-inducible CTU2 knockdown in HeLa cells over time. Duration of dox treatment is indicated in days. scrambled, non-targeting shRNA control. (b) APM-northern blot validation of tRNA thiolation loss in HeLa and RPE1 cells after 4 days of dox treatment. is shown representatively. (c) Viability analysis of CTU2 knockdown cell lines by Annexin V staining. +CTU2 res indicates shRNA-resistant CTU2 overexpression. One-way ANOVA, WT vs. CTU2 res p = 0.264. n = 3 technical replicates. (d) Western blot analysis of unfolded protein response (UPR) checkpoints during progressive tRNA hypothiolation in RPE1 cells. Time points indicate days of CTU2 depletion. (e) Western blot validation of selected proteins identified in DREAM-PL patient fibroblasts, analyzed under acute CTU2 knockdown conditions. (f) Ribosome profiling in 96 h CTU2 -depleted RPE1 cells compared with untreated controls. Normalized codon occupancy is shown for A- and G-ending codons of the indicated amino acids. Codons are divided into quartiles according to relative library abundance. n = 2 technical replicates. (g) Cumulative A-ending codon frequency (AAA, CAA, GAA, and AGA) in coding sequences (CDSs) of proteins quantified in CTU2 -depleted RPE1 cells after 96 hours. The dashed line indicates the average A-ending codon content (7.6%) across 19,085 analyzed human CDSs. Two-tailed unpaired t-test, p > 0.001 (down and up). (h) Gene Ontology (GO) enrichment analysis on the top 5% of human coding sequences ranked by A-ending codon content (AAA, CAA, GAA, AGA). Enriched pathways were clustered by GO Biological Process (2023). Cilium-associated terms are highlighted in orange. (i) Ribosome occupancy analysis of all mRNAs measured by ribosome profiling (f). Transcripts were ranked by A-ending codon frequency (AAA, CAA, GAA and AGA) and divided into ten equal bins (bin 1 = lowest, bin 10 = highest). A negative correlation was observed between A-ending codon frequency and ribosome abundance. ANOVA with post hoc test. Adjusted p-values are indicated. (j) Ciliogenesis assay in CTU2 -knockdown and wildtype RPE1 cells upon serum starvation. Representative immunofluorescence images show primary cilia (green) and nuclei (blue). The treatment scheme is shown above. Quantification of fractions of cilia-forming RPE1 cells under tRNA hypothiolation is provided. Two-tailed unpaired t-test, p-values are indicated. n = 3 technical replicates. Scale bars 10µm.

    Article Snippet: For CTU2 knockdown lines we utilized the GLTR system, an all-in-one lentiviral, doxycycline inducible short hairpin RNA (shRNA) expression vector (Addgene 55790, 58246).

    Techniques: Western Blot, Knockdown, shRNA, Control, Northern Blot, Biomarker Discovery, Staining, Over Expression, Two Tailed Test, Immunofluorescence

    Journal: eLife

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

    doi: 10.7554/eLife.74940

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , pENTR-D-Topo- hTRIM9 (plasmid) , , RRID: Addgene_51032 , pENTR expression vector.

    Techniques: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software