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pentr™ expression cassette pad/pl-dest™ gateway® vector  (Thermo Fisher)


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    Thermo Fisher pentr™ expression cassette pad/pl-dest™ gateway® vector
    Pentr™ Expression Cassette Pad/Pl Dest™ Gateway® Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pentr+expression+vector/pm31186476-159-2-10
    Average 90 stars, based on 1 article reviews
    pentr™ expression cassette pad/pl-dest™ gateway® vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: The Arabidopsis RING-Type E3 Ligase TEAR4 Controls Seed Germination by Targeting RGA for Degradation.
    Article Snippet: Light and DELLA proteins are central factors controlling seed germination which is critical for seed plant survival and agricultural production.. However, the mechanisms underlying DELLA degradation under different light conditions during seed germination remain to be clarified.. Here, it is reported that TIE1-ASSOCIATED RING-TYPE E3 LIGASE4 (TEAR4) and other TEARs redundantly promote DELLA degradation to positively regulate seed germination in Arabidopsis.

    Article Title: MIG6 loss increased RET inhibitor tolerant persister cells in RET-rearranged non-small cell lung cancer.
    Article Snippet: Recently approved RET tyrosine kinase inhibitors (TKIs) have shown promising therapeutic effects against RETrearranged non-small cell lung cancer (NSCLC) or RET-mutated thyroid cancer.. However, resistance develops, limiting long-term efficacy.. Although many RET-TKI resistance mechanisms, such as secondary mutations in RET or activation of bypass pathways, are known, some primary or acquired resistance mechanisms are unclear.

    Expressing:

    Article Title: MIG6 loss confers resistance to ALK/ROS1 inhibitors in NSCLC through EGFR activation by low-dose EGF.
    Article Snippet: .. Generating lentivirus and stable MIG6 expression in JFCR-168 cells. cDNA encoding MIG6 was amplified by PCR and cloned into a pENTR (Thermo Fisher Scientific) vector, then cloned into pLenti6.3 (Thermo Fisher Scientific) using LR clonase II. .. Lipofectamine 2000 (Thermo Fisher Scientific) was used to make lentivirus by transfecting pLenti6.3 construct in 293FT cells, following the manufacturer’s protocol.

    Amplification:

    Article Title: MIG6 loss confers resistance to ALK/ROS1 inhibitors in NSCLC through EGFR activation by low-dose EGF.
    Article Snippet: .. Generating lentivirus and stable MIG6 expression in JFCR-168 cells. cDNA encoding MIG6 was amplified by PCR and cloned into a pENTR (Thermo Fisher Scientific) vector, then cloned into pLenti6.3 (Thermo Fisher Scientific) using LR clonase II. .. Lipofectamine 2000 (Thermo Fisher Scientific) was used to make lentivirus by transfecting pLenti6.3 construct in 293FT cells, following the manufacturer’s protocol.

    Polymerase Chain Reaction:

    Article Title: MIG6 loss confers resistance to ALK/ROS1 inhibitors in NSCLC through EGFR activation by low-dose EGF.
    Article Snippet: .. Generating lentivirus and stable MIG6 expression in JFCR-168 cells. cDNA encoding MIG6 was amplified by PCR and cloned into a pENTR (Thermo Fisher Scientific) vector, then cloned into pLenti6.3 (Thermo Fisher Scientific) using LR clonase II. .. Lipofectamine 2000 (Thermo Fisher Scientific) was used to make lentivirus by transfecting pLenti6.3 construct in 293FT cells, following the manufacturer’s protocol.

    Article Title: NBR1-mediated selective autophagy of ARF7 modulates root branching.
    Article Snippet: Auxin dictates root architecture via the Auxin Response Factor (ARF) family of transcription factors, which control lateral root (LR) formation.. In Arabidopsis, ARF7 regulates the specification of prebranch sites (PBS) generating LRs through gene expression oscillations and plays a pivotal role during LR initiation.. Despite the importance of ARF7 in this process, there is a surprising lack of knowledge about how ARF7 turnover is regulated and how this impacts root architecture.

    Article Title: POLO kinase inhibits Protein Phosphatase 1 to promote the Spindle Assembly Checkpoint and prevent aneuploidy
    Article Snippet: To clone the PP1α87B into the pENTR plasmid (Gateway Cloning System), PP1α87B cDNA was used as template to amplify the PP1α87B coding sequence by PCR using Phusion polymerase (ThermoFisher Scientific). .. The PCR product was inserted into the pENTR (Invitrogen, Carlsbad, CA) by FastCloning ( ). ..

    Clone Assay:

    Article Title: MIG6 loss confers resistance to ALK/ROS1 inhibitors in NSCLC through EGFR activation by low-dose EGF.
    Article Snippet: .. Generating lentivirus and stable MIG6 expression in JFCR-168 cells. cDNA encoding MIG6 was amplified by PCR and cloned into a pENTR (Thermo Fisher Scientific) vector, then cloned into pLenti6.3 (Thermo Fisher Scientific) using LR clonase II. .. Lipofectamine 2000 (Thermo Fisher Scientific) was used to make lentivirus by transfecting pLenti6.3 construct in 293FT cells, following the manufacturer’s protocol.

    Article Title: COBRA-LIKE4 Modulates Cellulose Synthase Velocity and Facilitates Cellulose Deposition in the Secondary Cell Wall.
    Article Snippet: 1 1 COBRA-LIKE4 Modulates Cellulose Synthase Velocity and Facilitates Cellulose Deposition in 2 the Secondary Cell Wall 3 4 Running Title: COBL4 in Secondary Wall Cellulose Biosynthesis 5 6 7 Jan Xue, Grant McNair, Yoichiro Watanabe, Madison Kaplen, Sydne Guevara8 Rozo,Mathias Schuetz, Rene Schneider, Shawn D. Mansfield , and Lacey Samuels 9 10 1.. Department of Botany, University of British Columbia, Vancouver V6T 1Z4, Canada 11 2.. Department of Wood Sciences, University of British Columbia, Vancouver V6T 1Z4, 12 Canada 13 3.

    Article Title: NBR1-mediated selective autophagy of ARF7 modulates root branching.
    Article Snippet: Auxin dictates root architecture via the Auxin Response Factor (ARF) family of transcription factors, which control lateral root (LR) formation.. In Arabidopsis, ARF7 regulates the specification of prebranch sites (PBS) generating LRs through gene expression oscillations and plays a pivotal role during LR initiation.. Despite the importance of ARF7 in this process, there is a surprising lack of knowledge about how ARF7 turnover is regulated and how this impacts root architecture.

    Bimolecular Fluorescence Complementation Assay:

    Article Title: NBR1-mediated selective autophagy of ARF7 modulates root branching.
    Article Snippet: Auxin dictates root architecture via the Auxin Response Factor (ARF) family of transcription factors, which control lateral root (LR) formation.. In Arabidopsis, ARF7 regulates the specification of prebranch sites (PBS) generating LRs through gene expression oscillations and plays a pivotal role during LR initiation.. Despite the importance of ARF7 in this process, there is a surprising lack of knowledge about how ARF7 turnover is regulated and how this impacts root architecture.

    Generated:

    Article Title: O-GlcNAcylation of FOXK1 orchestrates the E2F pathway and promotes oncogenesis
    Article Snippet: .. His-OGT-Flag was generated by subcloning the OGT cDNA into pET30a+ vector (Novagen ® ). siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic ® ) and subcloned into pENTR (Life technologies ® ). .. GST-, FLAG- and MYC-tagged, retroviral pMSCV-Flag/HA (Addgene, #41033) and pMSCV-3Flag (generated for this study) constructs were generated using the Gateway recombination system (Life technologies).

    Subcloning:

    Article Title: O-GlcNAcylation of FOXK1 orchestrates the E2F pathway and promotes oncogenesis
    Article Snippet: .. His-OGT-Flag was generated by subcloning the OGT cDNA into pET30a+ vector (Novagen ® ). siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic ® ) and subcloned into pENTR (Life technologies ® ). .. GST-, FLAG- and MYC-tagged, retroviral pMSCV-Flag/HA (Addgene, #41033) and pMSCV-3Flag (generated for this study) constructs were generated using the Gateway recombination system (Life technologies).

    Synthesized:

    Article Title: O-GlcNAcylation of FOXK1 orchestrates the E2F pathway and promotes oncogenesis
    Article Snippet: .. His-OGT-Flag was generated by subcloning the OGT cDNA into pET30a+ vector (Novagen ® ). siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic ® ) and subcloned into pENTR (Life technologies ® ). .. GST-, FLAG- and MYC-tagged, retroviral pMSCV-Flag/HA (Addgene, #41033) and pMSCV-3Flag (generated for this study) constructs were generated using the Gateway recombination system (Life technologies).

    Plasmid Preparation:

    Article Title: O-GlcNAcylation of FOXK1 orchestrates the E2F pathway and promotes oncogenesis
    Article Snippet: .. His-OGT-Flag was generated by subcloning the OGT cDNA into pET30a+ vector (Novagen ® ). siRNA-resistant human FOXK1 and FOXK2 cDNAs were synthesized into a pBluescript plasmid (Biobasic ® ) and subcloned into pENTR (Life technologies ® ). .. GST-, FLAG- and MYC-tagged, retroviral pMSCV-Flag/HA (Addgene, #41033) and pMSCV-3Flag (generated for this study) constructs were generated using the Gateway recombination system (Life technologies).



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    Image Search Results


    (a) Western blot analysis of doxycycline (dox)-inducible CTU2 knockdown in HeLa cells over time. Duration of dox treatment is indicated in days. scrambled, non-targeting shRNA control. (b) APM-northern blot validation of tRNA thiolation loss in HeLa and RPE1 cells after 4 days of dox treatment. is shown representatively. (c) Viability analysis of CTU2 knockdown cell lines by Annexin V staining. +CTU2 res indicates shRNA-resistant CTU2 overexpression. One-way ANOVA, WT vs. CTU2 res p = 0.264. n = 3 technical replicates. (d) Western blot analysis of unfolded protein response (UPR) checkpoints during progressive tRNA hypothiolation in RPE1 cells. Time points indicate days of CTU2 depletion. (e) Western blot validation of selected proteins identified in DREAM-PL patient fibroblasts, analyzed under acute CTU2 knockdown conditions. (f) Ribosome profiling in 96 h CTU2 -depleted RPE1 cells compared with untreated controls. Normalized codon occupancy is shown for A- and G-ending codons of the indicated amino acids. Codons are divided into quartiles according to relative library abundance. n = 2 technical replicates. (g) Cumulative A-ending codon frequency (AAA, CAA, GAA, and AGA) in coding sequences (CDSs) of proteins quantified in CTU2 -depleted RPE1 cells after 96 hours. The dashed line indicates the average A-ending codon content (7.6%) across 19,085 analyzed human CDSs. Two-tailed unpaired t-test, p > 0.001 (down and up). (h) Gene Ontology (GO) enrichment analysis on the top 5% of human coding sequences ranked by A-ending codon content (AAA, CAA, GAA, AGA). Enriched pathways were clustered by GO Biological Process (2023). Cilium-associated terms are highlighted in orange. (i) Ribosome occupancy analysis of all mRNAs measured by ribosome profiling (f). Transcripts were ranked by A-ending codon frequency (AAA, CAA, GAA and AGA) and divided into ten equal bins (bin 1 = lowest, bin 10 = highest). A negative correlation was observed between A-ending codon frequency and ribosome abundance. ANOVA with post hoc test. Adjusted p-values are indicated. (j) Ciliogenesis assay in CTU2 -knockdown and wildtype RPE1 cells upon serum starvation. Representative immunofluorescence images show primary cilia (green) and nuclei (blue). The treatment scheme is shown above. Quantification of fractions of cilia-forming RPE1 cells under tRNA hypothiolation is provided. Two-tailed unpaired t-test, p-values are indicated. n = 3 technical replicates. Scale bars 10µm.

    Journal: bioRxiv

    Article Title: tRNA thiolation defects disrupt cellular proteostasis and tissue homeostasis in mammals

    doi: 10.1101/2025.10.24.684405

    Figure Lengend Snippet: (a) Western blot analysis of doxycycline (dox)-inducible CTU2 knockdown in HeLa cells over time. Duration of dox treatment is indicated in days. scrambled, non-targeting shRNA control. (b) APM-northern blot validation of tRNA thiolation loss in HeLa and RPE1 cells after 4 days of dox treatment. is shown representatively. (c) Viability analysis of CTU2 knockdown cell lines by Annexin V staining. +CTU2 res indicates shRNA-resistant CTU2 overexpression. One-way ANOVA, WT vs. CTU2 res p = 0.264. n = 3 technical replicates. (d) Western blot analysis of unfolded protein response (UPR) checkpoints during progressive tRNA hypothiolation in RPE1 cells. Time points indicate days of CTU2 depletion. (e) Western blot validation of selected proteins identified in DREAM-PL patient fibroblasts, analyzed under acute CTU2 knockdown conditions. (f) Ribosome profiling in 96 h CTU2 -depleted RPE1 cells compared with untreated controls. Normalized codon occupancy is shown for A- and G-ending codons of the indicated amino acids. Codons are divided into quartiles according to relative library abundance. n = 2 technical replicates. (g) Cumulative A-ending codon frequency (AAA, CAA, GAA, and AGA) in coding sequences (CDSs) of proteins quantified in CTU2 -depleted RPE1 cells after 96 hours. The dashed line indicates the average A-ending codon content (7.6%) across 19,085 analyzed human CDSs. Two-tailed unpaired t-test, p > 0.001 (down and up). (h) Gene Ontology (GO) enrichment analysis on the top 5% of human coding sequences ranked by A-ending codon content (AAA, CAA, GAA, AGA). Enriched pathways were clustered by GO Biological Process (2023). Cilium-associated terms are highlighted in orange. (i) Ribosome occupancy analysis of all mRNAs measured by ribosome profiling (f). Transcripts were ranked by A-ending codon frequency (AAA, CAA, GAA and AGA) and divided into ten equal bins (bin 1 = lowest, bin 10 = highest). A negative correlation was observed between A-ending codon frequency and ribosome abundance. ANOVA with post hoc test. Adjusted p-values are indicated. (j) Ciliogenesis assay in CTU2 -knockdown and wildtype RPE1 cells upon serum starvation. Representative immunofluorescence images show primary cilia (green) and nuclei (blue). The treatment scheme is shown above. Quantification of fractions of cilia-forming RPE1 cells under tRNA hypothiolation is provided. Two-tailed unpaired t-test, p-values are indicated. n = 3 technical replicates. Scale bars 10µm.

    Article Snippet: For CTU2 knockdown lines we utilized the GLTR system, an all-in-one lentiviral, doxycycline inducible short hairpin RNA (shRNA) expression vector (Addgene 55790, 58246).

    Techniques: Western Blot, Knockdown, shRNA, Control, Northern Blot, Biomarker Discovery, Staining, Over Expression, Two Tailed Test, Immunofluorescence

    Journal: eLife

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

    doi: 10.7554/eLife.74940

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , pENTR-D-Topo- hTRIM9 (plasmid) , , RRID: Addgene_51032 , pENTR expression vector.

    Techniques: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software