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eukaryotic expression vector pegfp c1 pp1α  (Addgene inc)


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    Addgene inc eukaryotic expression vector pegfp c1 pp1α
    Eukaryotic Expression Vector Pegfp C1 Pp1α, supplied by Addgene inc, used in various techniques. Bioz Stars score: 91/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfpc1+expression+vector/pEGFP(C1)-PP1alpha+(Plasmid+%2344224)/pmc05847313-248-1-8
    Average 91 stars, based on 14 article reviews
    eukaryotic expression vector pegfp c1 pp1α - by Bioz Stars, 2026-10
    91/100 stars

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    Plasmid Preparation:

    Article Title: Non‐ionotropic NMDAR signalling activates Panx1 to induce P2X4R‐dependent long‐term depression in the hippocampus
    Article Snippet: .. Enhanced green fluorescent protein plasmid (pEGFP‐C1) was commercially purchased (Addgene, Watertown, MA, USA). .. HEK293T cells (ATCC, Manassas, VA, USA) were maintained in Dulbecco's modified Eagle's medium, high glucose, supplemented with 10% foetal bovine serum, 1% penicillin and 1% streptomycin, and housed in a humidified cell culture incubator with 95% O 2 and 5% CO 2 at 37°C.

    Article Title: Pannexin-1 channel inhibition alleviates opioid withdrawal in rodents by modulating locus coeruleus to spinal cord circuitry.
    Article Snippet: Rattus norvegicus Pannexin-1 (rPanx1) complementary DNA (cDNA) was cloned between BamHI and SalI sites in a pRK5 expression vector (pRK5-rPanx1). .. Enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1) was originally commercially purchased (Addgene, discontinued). ..

    Article Title: Non‐ionotropic NMDAR signalling activates Panx1 to induce P2X4R‐dependent long‐term depression in the hippocampus
    Article Snippet: .. Enhanced green fluorescent protein plasmid (pEGFP-C1) was commercially purchased (Addgene, Watertown, MA, USA). .. HEK293T cells (ATCC, Manassas, VA, USA) were maintained in Dulbecco’s modified Eagle’s medium, high glucose, supplemented with 10% foetal bovine serum, 1% penicillin and 1% streptomycin, and housed in a humidified cell culture incubator with 95% O2 and 5% CO2 at 37°C.

    Article Title: Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention
    Article Snippet: .. The cargo-binding domain of TNPO3 (CBDT) plasmid was a kind gift from the Shav-Tal Lab . GFP-phosphatase plasmids were a kind gift from the Bodenmiller Lab . pCMV-hyPBase was a kind gift from the Wellcome Trust Sanger Institute . pECFP(C1)-NIPP1 (Addgene plasmid # 44226; http://n2t.net/addgene:44226 ; RRID:Addgene_44226), pEGFP(C1)-PP1alpha (Addgene plasmid # 44224; http://n2t.net/addgene:44224 ; RRID:Addgene_44224), pEGFP(N3)-PP1beta (Addgene plasmid # 44223; http://n2t.net/addgene:44223 ; RRID:Addgene_44223), and pEYFP(C1)-PP1gamma (Addgene plasmid # 44230; http://n2t.net/addgene:44230 ; RRID:Addgene_44230) were gifts from Angus Lamond & Laura Trinkle-Mulcahy. .. AICSDP-82:SON-mEGFP was a gift from Allen Institute for Cell Science (Addgene plasmid # 133964; http://n2t.net/addgene:133964 ; RRID:Addgene_133964).

    Article Title: The Pannexin-1 N-terminal Helix Gates a Switch between Ion Conductance and Anandamide Transport
    Article Snippet: Homo sapiens Pannexin-1 (hPanx1) cDNA was cloned between Nhe1 and Xho1 in a pcDNA3.1(-) expression vector (pcDNA3.1(-) – hPanx1). .. Enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1) was commercially purchased (Addgene). ..

    Article Title: Pannexin-1 channel inhibition alleviates opioid withdrawal in rodents by modulating locus coeruleus to spinal cord circuitry
    Article Snippet: Rattus norvegicus Pannexin-1 (rPanx1) complementary DNA (cDNA) was cloned between BamHI and SalI sites in a pRK5 expression vector (pRK5-rPanx1). .. Enhanced green fluorescent protein (EGFP) plasmid (pEGFP-C1) was originally commercially purchased (Addgene, discontinued). ..

    Article Title: Phosphorylation of a nuclear condensate regulates cohesion and mRNA retention.
    Article Snippet: .. GFP-phosphatase plasmids were a kind gift from the Bodenmiller Lab33. pCMV-hyPBase was a kind gift from the Wellcome Trust Sanger Institute80. pECFP(C1)-NIPP1 (Addgene plasmid # 44226; http://n2t.net/addgene:44226; RRID:Addgene_44226), pEGFP(C1)-PP1alpha (Addgene plasmid # 44224; http://n2t.net/addgene:44224; RRID:Addgene_44224), pEGFP(N3)PP1beta (Addgene plasmid # 44223; http://n2t.net/addgene:44223; RRID:Addgene_44223), and pEYFP(C1)-PP1gamma (Addgene plasmid # 44230; http://n2t.net/addgene:44230; RRID:Addgene_44230) were gifts from Angus Lamond & Laura Trinkle-Mulcahy. .. AICSDP-82:SONmEGFP was a gift from Allen Institute for Cell Science (Addgene plasmid# 133964; http://n2t.net/addgene:133964; RRID:Addgene_133964).



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    Image Search Results


    Journal: eLife

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

    doi: 10.7554/eLife.74940

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , pEGFPC1 expression vector.

    Techniques: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software

    Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.

    Journal: Oncotarget

    Article Title: Syntaxin-1/TI-VAMP SNAREs interact with Trk receptors and are required for neurotrophin-dependent outgrowth.

    doi: 10.18632/oncotarget.26307

    Figure Lengend Snippet: Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.

    Article Snippet: Full-length Sytx1A or truncated DNAs (Sytx1 ACYT, Sytx1AHabc, Sytx1AH3TM, Sytx1AH3, and Sytx1ATM) were cloned into EcoRI sites in pEGFPC1 fusion mammalian protein expression vector (Clontech), as described [18].

    Techniques: Immunoprecipitation, Transfection