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expression vector pegfpc1  (TaKaRa)


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    Structured Review

    TaKaRa expression vector pegfpc1
    Expression Vector Pegfpc1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 619 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfpc1+expression+vector/pmCherry+Vector/pm24441103-183-27-30
    Average 96 stars, based on 619 article reviews
    expression vector pegfpc1 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Gut Commensal Klebsiella pneumoniae Extracellular Vesicles Shape a Liver Microenvironment Conducive to Gut-Liver Bacterial Translocation and Pro-Tumorigenic Processes.
    Article Snippet: .. A pmCherry plasmid (Clontech, Palo Alto, CA, USA, cat. no. 632522) (Tsugawa et al. 2023 ) or pGFP plasmid (Clontech, cat. no. 632370) with an elongation factor Tu (TufA) from K. pneumoniae insert (pGFP::tufa) were electroporated into K. pneumoniae ATCC43816 using a MicroPulser (Bio-Rad, Hercules, CA, USA). ..

    Article Title: Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation.
    Article Snippet: .. The pmCherry vector was obtained from Takara Bio Company (CA, USA). ..

    Article Title: Lipid Droplet Degradation Through Lipophagy in Aurantiochytrium limacinum mh0186.
    Article Snippet: Autophagy is a cellular recycling process degrading and reusing cytoplasmic components via lysosomes or vacuoles, whereas lipophagy is a specialized form of autophagy that degrades lipid droplets (LDs).. Thraustochytrids are heterotrophic marine protists known for high polyunsaturated fatty acid (PUFA) production and as valuable models for lipid metabolism research.. In this study, LD degradation in Aurantiochytrium limacinum mh0186 was characterized under glucose-starvation conditions.

    Article Title: Optimizing Alternating Current Electrical Stimulation Parameters to Enhance Osteoblasts Differentiation
    Article Snippet: .. The pmCherry vector was obtained from Takara Bio Company (CA, USA). ..

    Article Title: Different mCherry isoforms show distinct photophysical properties in FRET tandem constructs
    Article Snippet: .. All used fluorescent constructs were PCR amplified from a pmCherry vector (Clontech Laboratories Inc., United States of America) using distinct oligonucleotide primers each (see for details). ..

    Article Title: Selective induction by statins of FAM134B-mediated sarcoplasmic reticulum (SR)-phagy degrades the SR calcium pump SERCA1 and contributes to myopathy
    Article Snippet: The coding sequence of mouse SERCA1 was cloned into p3XFlag-CMV-7.1 vector (Sigma) at the NotI/XbaI site to generate pFLAG-SERCA1. .. To generate EGFP-SERCA1 and pmCherry-EGFP-SERCA1 expression plasmids, coding region of mouse SERCA1 was first cloned into the pEGFP-C1 vector (Clonetech) at the XhoI/SacII sites, and then EGFP-SERCA1 was subcloned into pmCherry-C1 vector (Clonetech) at SacII/XmaI sites. .. The coding sequence of mouse FAM134B-S cDNA was cloned into p3XFlag-CMV-7.1 vector (Sigma) at the HindIII/EcoRI site to generate pFLAG-FAM134B-S, or cloned into pmCherry-C1 vector at the HindIII/EcoRI site to generate pmCherry-FAM134B-S.

    Clone Assay:

    Article Title: DA-Raf synergistically binds to the plasma membrane and Ras to suppress ERK signaling
    Article Snippet: .. cDNAs encoding mouse K-Ras, DA-Raf, A-Raf, B-Raf, human C-Raf, and mouse 14-3-3β were cloned by PCR and inserted into pEGFP, pmCherry (Clontech), pTagBFP (Evrogen), phmKGN, phmKGC vectors (ColalHue Fluo-chase kit, MBL). ..

    Article Title: Selective induction by statins of FAM134B-mediated sarcoplasmic reticulum (SR)-phagy degrades the SR calcium pump SERCA1 and contributes to myopathy
    Article Snippet: The coding sequence of mouse SERCA1 was cloned into p3XFlag-CMV-7.1 vector (Sigma) at the NotI/XbaI site to generate pFLAG-SERCA1. .. To generate EGFP-SERCA1 and pmCherry-EGFP-SERCA1 expression plasmids, coding region of mouse SERCA1 was first cloned into the pEGFP-C1 vector (Clonetech) at the XhoI/SacII sites, and then EGFP-SERCA1 was subcloned into pmCherry-C1 vector (Clonetech) at SacII/XmaI sites. .. The coding sequence of mouse FAM134B-S cDNA was cloned into p3XFlag-CMV-7.1 vector (Sigma) at the HindIII/EcoRI site to generate pFLAG-FAM134B-S, or cloned into pmCherry-C1 vector at the HindIII/EcoRI site to generate pmCherry-FAM134B-S.

    Polymerase Chain Reaction:

    Article Title: DA-Raf synergistically binds to the plasma membrane and Ras to suppress ERK signaling
    Article Snippet: .. cDNAs encoding mouse K-Ras, DA-Raf, A-Raf, B-Raf, human C-Raf, and mouse 14-3-3β were cloned by PCR and inserted into pEGFP, pmCherry (Clontech), pTagBFP (Evrogen), phmKGN, phmKGC vectors (ColalHue Fluo-chase kit, MBL). ..

    Article Title: Different mCherry isoforms show distinct photophysical properties in FRET tandem constructs
    Article Snippet: .. All used fluorescent constructs were PCR amplified from a pmCherry vector (Clontech Laboratories Inc., United States of America) using distinct oligonucleotide primers each (see for details). ..

    Amplification:

    Article Title: Lipid Droplet Degradation Through Lipophagy in Aurantiochytrium limacinum mh0186.
    Article Snippet: Autophagy is a cellular recycling process degrading and reusing cytoplasmic components via lysosomes or vacuoles, whereas lipophagy is a specialized form of autophagy that degrades lipid droplets (LDs).. Thraustochytrids are heterotrophic marine protists known for high polyunsaturated fatty acid (PUFA) production and as valuable models for lipid metabolism research.. In this study, LD degradation in Aurantiochytrium limacinum mh0186 was characterized under glucose-starvation conditions.

    Article Title: Different mCherry isoforms show distinct photophysical properties in FRET tandem constructs
    Article Snippet: .. All used fluorescent constructs were PCR amplified from a pmCherry vector (Clontech Laboratories Inc., United States of America) using distinct oligonucleotide primers each (see for details). ..

    Construct:

    Article Title: Different mCherry isoforms show distinct photophysical properties in FRET tandem constructs
    Article Snippet: .. All used fluorescent constructs were PCR amplified from a pmCherry vector (Clontech Laboratories Inc., United States of America) using distinct oligonucleotide primers each (see for details). ..

    Expressing:

    Article Title: Selective induction by statins of FAM134B-mediated sarcoplasmic reticulum (SR)-phagy degrades the SR calcium pump SERCA1 and contributes to myopathy
    Article Snippet: The coding sequence of mouse SERCA1 was cloned into p3XFlag-CMV-7.1 vector (Sigma) at the NotI/XbaI site to generate pFLAG-SERCA1. .. To generate EGFP-SERCA1 and pmCherry-EGFP-SERCA1 expression plasmids, coding region of mouse SERCA1 was first cloned into the pEGFP-C1 vector (Clonetech) at the XhoI/SacII sites, and then EGFP-SERCA1 was subcloned into pmCherry-C1 vector (Clonetech) at SacII/XmaI sites. .. The coding sequence of mouse FAM134B-S cDNA was cloned into p3XFlag-CMV-7.1 vector (Sigma) at the HindIII/EcoRI site to generate pFLAG-FAM134B-S, or cloned into pmCherry-C1 vector at the HindIII/EcoRI site to generate pmCherry-FAM134B-S.



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    Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with <t>pEGFPC1-TrkB.</t> (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.
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    Image Search Results


    Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.

    Journal: Oncotarget

    Article Title: Syntaxin-1/TI-VAMP SNAREs interact with Trk receptors and are required for neurotrophin-dependent outgrowth.

    doi: 10.18632/oncotarget.26307

    Figure Lengend Snippet: Figure 2: Co-immunoprecipitation experiments in HEK293 cells transfected with (A) pEF-BOS-SNAP25-FLAG alone or together with pEGFPC1-TrkB. (B) pEF-BOS-VAMP2-FLAG alone or together with pEGFPC1-TrkB. (C) pEF-BOS-SNAP25-FLAG, pEF-BOS- VAMP2-FLAG and TrkA-HA. (D) pEF-BOS-SNAP25-FLAG, pEF-BOS-VAMP2-FLAG and TrkC-myc. No co-immunoprecipitation was observed between proteins analyzed with anti α-GFP, anti-myc, anti-FLAG, or anti-HA antibodies. Two transfections in HEK cells per condition were run in parallel for each experiment and three experiments were done. Arrows indicate specific bands.

    Article Snippet: Full-length Sytx1A or truncated DNAs (Sytx1 ACYT, Sytx1AHabc, Sytx1AH3TM, Sytx1AH3, and Sytx1ATM) were cloned into EcoRI sites in pEGFPC1 fusion mammalian protein expression vector (Clontech), as described [18].

    Techniques: Immunoprecipitation, Transfection

    Journal: eLife

    Article Title: SAFB regulates hippocampal stem cell fate by targeting Drosha to destabilize Nfib mRNA

    doi: 10.7554/eLife.74940

    Figure Lengend Snippet:

    Article Snippet: Recombinant DNA reagent , pEGFPC1-6XHis- FLKSRP (plasmid) , , RRID: Addgene_23001 , pEGFPC1 expression vector.

    Techniques: Protease Inhibitor, Recombinant, Clone Assay, Bicinchoninic Acid Protein Assay, Mutagenesis, Transfection, Sequencing, esiRNA, Blocking Assay, Plasmid Preparation, Expressing, Software