hek293 cells (Alomone Labs)
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93
Structured Review
Alomone Labs
hek293 cells
Hek293 Cells, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+expression+vector/Anti-TRPM6+(extracellular)+Antibody/pmc05813043-441-9-43
Average 93 stars, based on 14 article reviews
Hek293 Cells, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfpc1+expression+vector/Anti-TRPM6+(extracellular)+Antibody/pmc05813043-441-9-43
Average 93 stars, based on 14 article reviews
hek293 cells - by Bioz Stars,
2026-11
93/100 stars
Images
1) Product Images from "Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry"
Article Title: Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry
Journal: Scientific Reports
doi: 10.1038/s41598-018-21004-w
Figure Legend Snippet: TRPM7/TRPM6 protein expression and TRPM7 kinase activity in splenic T cells. ( A ) Western blot analysis of immunoprecipitated TRPM7 from whole cell lysates of WT and KD splenic T cells. T cells were stimulated with PMA/ionomycin or anti-CD3/CD28 antibody coated beads for 48 hrs. Mouse embryonic fibroblasts were used as a positive control. Equal amounts of protein before immunoprecipitation were ensured by probing for actin. ( B ) Incorporation of 32 P into exogenous myelin basic protein (MBP) by TRPM7 immunoprecipitated from WT and KD resting T cells. Equal quantities of MBP were verified by coomassie blue staining. ( C ) Control experiment showing that anti-TRPM6 antibody was able to recognize TRPM6, by immunoprecipitation using anti-TRPM6 antibody in GFP-TRPM6 transfected HEK cells ( D ). Western blot analysis of TRPM6 immunoprecipitated from WT and KD mouse T cells and kidneys. Full gel images are provided in Supplementary Fig. .
Techniques Used: Expressing, Activity Assay, Western Blot, Immunoprecipitation, Positive Control, Staining, Transfection
Related Articles
other:Article Title: Characterization of intestine-specific TRPM6 knockout C57BL/6 J mice: effects of short-term omeprazole treatment. Article Snippet: To transfect the cells with mock, WT, or mutant SLC30A10, Lipofectamine 2000 (Invitrogen) was used at 1:2 DNA:Lipofectamine ratio. Article Title: Characterization of intestine-specific TRPM6 knockout C57BL/6 J mice: effects of short-term omeprazole treatment Article Snippet: HEK293 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, Lonza) containing 10% (v/v) fetal calf serum (VWR International), 2 mM L-glutamine (Sigma-Aldrich), and 10 μg/mL nonessential amino acids (Sigma-Aldrich) at 37 °C and 5% (v/v) CO 2 . Incubation:Article Title: TRPM6 in murine kidneys—of targets and antibodies Article Snippet: .. Sections were incubated with primary antiserum (polyclonal Article Title: TRPM6 in murine kidneys-of targets and antibodies. Article Snippet: .. Sections were incubated with primary antiserum (polyclonal |
