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pegfp n1 empty vector  (TaKaRa)


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    Structured Review

    TaKaRa pegfp n1 empty vector
    Pegfp N1 Empty Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+empty+vector/pEF1alpha-AcGFP1-N1+Vector/pmc03293851-168-23-28
    Average 95 stars, based on 7 article reviews
    pegfp n1 empty vector - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    Amplification:

    Article Title: An Inducible Expression System to Measure Rhodopsin Transport in Transgenic Xenopus Rod Outer Segments
    Article Snippet: .. The Gal4-VP16-GR module (consisting of 147 amino acids of S. cerevisiae Gal4 N-terminal transactivation domain, 59 amino acids of herpes viral protein VP16 and 266 amino acids of rat glucocorticoid receptor protein C terminal domain) was amplified from TOPtk-iGFP plasmid [ ] and inserted into peGFP-N1 vector (Clonetech, Mountain View, CA) with poly-Gly linker (LEPLEGTGGGGG) to create the pCMV:G3 plasmid. ..

    Plasmid Preparation:

    Article Title: An Inducible Expression System to Measure Rhodopsin Transport in Transgenic Xenopus Rod Outer Segments
    Article Snippet: .. The Gal4-VP16-GR module (consisting of 147 amino acids of S. cerevisiae Gal4 N-terminal transactivation domain, 59 amino acids of herpes viral protein VP16 and 266 amino acids of rat glucocorticoid receptor protein C terminal domain) was amplified from TOPtk-iGFP plasmid [ ] and inserted into peGFP-N1 vector (Clonetech, Mountain View, CA) with poly-Gly linker (LEPLEGTGGGGG) to create the pCMV:G3 plasmid. ..

    Article Title: GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation
    Article Snippet: .. Full-length human GTPBP8 coding sequence (GenBank accession no. NM_014170.2) in the pENTR221 vector (ORFeome Library; Biocentrum Helsinki Genome Biology Unit) was cloned with XhoI and BamHl restriction sites into the pEGFP-N1 vector (Takara Bio Inc.). ..

    Article Title: Dimerization of the C-type lectin-like receptor CD93 promotes its binding to Multimerin-2 in endothelial cells.
    Article Snippet: .. Blocking the signaling activated by the plasma membrane receptor CD93 has recently been demonstrated a useful tool in antiangiogenic treatment and oncotherapy.. In the proliferating endothelium, CD93 regulates cell adhesion, migration, and vascular maturation, yet it is unclear how CD93 interacts with the extracellular matrix activating signaling pathways involved in the vascular remodeling.. Here for the first time we show that in endothelial cells CD93 is structured as a dimer and that this oligomeric form is physiologically instrumental for the binding of CD93 to its ligand Multimerin-2. .. Blocking the signaling activated by the plasma membrane receptor CD93 has recently been demonstrated a useful tool in antiangiogenic treatment and oncotherapy.. In the proliferating endothelium, CD93 regulates cell adhesion, migration, and vascular maturation, yet it is unclear how CD93 interacts with the extracellular matrix activating signaling pathways involved in the vascular remodeling.. Here for the first time we show that in endothelial cells CD93 is structured as a dimer and that this oligomeric form is physiologically instrumental for the binding of CD93 to its ligand Multimerin-2.

    Article Title: MiR146a modulates chondrogenesis of bone marrow mesenchymal stem cells by modulating Lsm11 expression.
    Article Snippet: .. To identify the direct targets of miR146a, full-length mRNA encoding the mouse U7 snRNP-specific Sm-like protein LSM11 (Lsm11; NCBI gene ID 72290) was cloned into the pEGFP-N1 vector (Takara Bio USA, Inc., Mountain View, CA). siRNAs targeting mouse Lsm11 were purchased from GenePharma Co. Ltd. (Suzhou, China). .. Bone marrow MSCs were transfected using Lipofectamine 2000 (Invitrogen) in serum-free standard cell culture medium.

    Article Title: Plasmodium sporozoites require the protein B9 to invade hepatocytes
    Article Snippet: pCEN-SPECT2 plasmid , Iwanaga et al., 2010 , N/A. .. pEF1α-AcGFP1-N1 plasmid , Clontech , Cat# 631973. ..

    Sequencing:

    Article Title: Cyclin B1/Cdk1 Phosphorylation of Mitochondrial p53 Induces Anti-Apoptotic Response
    Article Snippet: .. pEGFP-N1 vector from Clontech (Mountain View, CA) (Accession # U55762) was used to insert the mitochondrial targeting sequence from Cox8a following by cyclin B1 or Cdk1 gene. ..

    Article Title: GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation
    Article Snippet: .. Full-length human GTPBP8 coding sequence (GenBank accession no. NM_014170.2) in the pENTR221 vector (ORFeome Library; Biocentrum Helsinki Genome Biology Unit) was cloned with XhoI and BamHl restriction sites into the pEGFP-N1 vector (Takara Bio Inc.). ..

    Article Title: Dimerization of the C-type lectin-like receptor CD93 promotes its binding to Multimerin-2 in endothelial cells.
    Article Snippet: .. Blocking the signaling activated by the plasma membrane receptor CD93 has recently been demonstrated a useful tool in antiangiogenic treatment and oncotherapy.. In the proliferating endothelium, CD93 regulates cell adhesion, migration, and vascular maturation, yet it is unclear how CD93 interacts with the extracellular matrix activating signaling pathways involved in the vascular remodeling.. Here for the first time we show that in endothelial cells CD93 is structured as a dimer and that this oligomeric form is physiologically instrumental for the binding of CD93 to its ligand Multimerin-2. .. Blocking the signaling activated by the plasma membrane receptor CD93 has recently been demonstrated a useful tool in antiangiogenic treatment and oncotherapy.. In the proliferating endothelium, CD93 regulates cell adhesion, migration, and vascular maturation, yet it is unclear how CD93 interacts with the extracellular matrix activating signaling pathways involved in the vascular remodeling.. Here for the first time we show that in endothelial cells CD93 is structured as a dimer and that this oligomeric form is physiologically instrumental for the binding of CD93 to its ligand Multimerin-2.

    Clone Assay:

    Article Title: GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation
    Article Snippet: .. Full-length human GTPBP8 coding sequence (GenBank accession no. NM_014170.2) in the pENTR221 vector (ORFeome Library; Biocentrum Helsinki Genome Biology Unit) was cloned with XhoI and BamHl restriction sites into the pEGFP-N1 vector (Takara Bio Inc.). ..

    Article Title: MiR146a modulates chondrogenesis of bone marrow mesenchymal stem cells by modulating Lsm11 expression.
    Article Snippet: .. To identify the direct targets of miR146a, full-length mRNA encoding the mouse U7 snRNP-specific Sm-like protein LSM11 (Lsm11; NCBI gene ID 72290) was cloned into the pEGFP-N1 vector (Takara Bio USA, Inc., Mountain View, CA). siRNAs targeting mouse Lsm11 were purchased from GenePharma Co. Ltd. (Suzhou, China). .. Bone marrow MSCs were transfected using Lipofectamine 2000 (Invitrogen) in serum-free standard cell culture medium.

    Fluorescence:

    Article Title: Dimerization of the C-type lectin-like receptor CD93 promotes its binding to Multimerin-2 in endothelial cells.
    Article Snippet: .. Blocking the signaling activated by the plasma membrane receptor CD93 has recently been demonstrated a useful tool in antiangiogenic treatment and oncotherapy.. In the proliferating endothelium, CD93 regulates cell adhesion, migration, and vascular maturation, yet it is unclear how CD93 interacts with the extracellular matrix activating signaling pathways involved in the vascular remodeling.. Here for the first time we show that in endothelial cells CD93 is structured as a dimer and that this oligomeric form is physiologically instrumental for the binding of CD93 to its ligand Multimerin-2. .. Blocking the signaling activated by the plasma membrane receptor CD93 has recently been demonstrated a useful tool in antiangiogenic treatment and oncotherapy.. In the proliferating endothelium, CD93 regulates cell adhesion, migration, and vascular maturation, yet it is unclear how CD93 interacts with the extracellular matrix activating signaling pathways involved in the vascular remodeling.. Here for the first time we show that in endothelial cells CD93 is structured as a dimer and that this oligomeric form is physiologically instrumental for the binding of CD93 to its ligand Multimerin-2.

    other:

    Article Title: Activation of c-Met and Upregulation of CD44 Expression Are Associated with the Metastatic Phenotype in the Colorectal Cancer Liver Metastasis Model
    Article Snippet: HT29 cells and Human Lung Microvascular Endothelial Cells (HMVEC-L) were obtained from American Type Culture Collection (Manassas, VA), were previously authenticated in November 2011 by Genetica DNA Laboratories (Cincinnati, OH) were cultured in McCoy’s 5A medium, purchased from Sigma Aldrich (St. Louis, MO) supplemented with 10% FBS and antibiotic-antimycotic.



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