empty pegfp c1 vector (TaKaRa)
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empty pegfp c1 vector
Empty Pegfp C1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+empty+vector/pm34502296-316-0-3
Average 86 stars, based on 1 article reviews
Empty Pegfp C1 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pegfp+empty+vector/pm34502296-316-0-3
Average 86 stars, based on 1 article reviews
empty pegfp c1 vector - by Bioz Stars,
2026-10
86/100 stars
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Polymerase Chain Reaction:Article Title: Enhanced control of RNA modification and CRISPR-Cas activity through redox-triggered disulfide cleavage. Article Snippet: Chemical RNA modification has emerged as a flexible approach for post-synthetic modifications in chemical biology research.. Guide RNA (gRNA) plays a crucial role in the clustered regularly interspaced short palindromic repeats and associated protein system (CRISPR-Cas).. Several toolkits have been developed to regulate gene expression and editing through modifications of gRNA. Article Title: Mitochondrial chaperon TNF-receptor- associated protein 1 as a novel apoptotic regulator conferring susceptibility to Pneumocystis jirovecii pneumonia Article Snippet: To generate EGFP-TRAP1 mammalian expression construct (pEGFP-TRAP1), human wild type TRAP1 cDNA was PCR amplified from a clone from the TOH7500-Human-Lentiviral-ORF-Expression-Library (TransOMIC Technologies, Inc.). .. EGFP-TRAP1 plasmid constructs with individual A64T (pEGFP-TRAP1-A64T), E93Q (pEGFP-TRAP1-E93Q), A64T (pEGFP-TRAP1-A64T), and E93Q (pEGFP-TRAP1-E93Q) mutations were created by overlap extension PCR protocol and assembled into XhoI – KpnI site of the Amplification:Article Title: Enhanced control of RNA modification and CRISPR-Cas activity through redox-triggered disulfide cleavage. Article Snippet: Chemical RNA modification has emerged as a flexible approach for post-synthetic modifications in chemical biology research.. Guide RNA (gRNA) plays a crucial role in the clustered regularly interspaced short palindromic repeats and associated protein system (CRISPR-Cas).. Several toolkits have been developed to regulate gene expression and editing through modifications of gRNA. Article Title: EPHA2 is a novel cell surface marker of OCT4-positive undifferentiated cells during the differentiation of mouse and human pluripotent stem cells Article Snippet: The PCR reaction was performed with LA-Taq (Takara). .. The amplified fragment was subcloned into AseI and NheI sites of a promoter-less enhanced green fluorescent protein expressing vector, Transfection:Article Title: A novel role for CSA in the regulation of nuclear envelope integrity: uncovering a non-canonical function Article Snippet: The next day, transfection of plasmid constructs was performed using Trans-IT 2020 reagent (#MIR 5400; Mirus Bio) following the manufacturer’s protocols. .. 24 h after transfection, experiments were performed. Plasmid Preparation:Article Title: A novel role for CSA in the regulation of nuclear envelope integrity: uncovering a non-canonical function Article Snippet: The next day, transfection of plasmid constructs was performed using Trans-IT 2020 reagent (#MIR 5400; Mirus Bio) following the manufacturer’s protocols. .. 24 h after transfection, experiments were performed. Article Title: Rit1-TBC1D10B signaling modulates FcγR-mediated phagosome formation in RAW264 macrophages. Article Snippet: .. The fragments were cloned into Article Title: Mitochondrial chaperon TNF-receptor- associated protein 1 as a novel apoptotic regulator conferring susceptibility to Pneumocystis jirovecii pneumonia Article Snippet: To generate EGFP-TRAP1 mammalian expression construct (pEGFP-TRAP1), human wild type TRAP1 cDNA was PCR amplified from a clone from the TOH7500-Human-Lentiviral-ORF-Expression-Library (TransOMIC Technologies, Inc.). .. EGFP-TRAP1 plasmid constructs with individual A64T (pEGFP-TRAP1-A64T), E93Q (pEGFP-TRAP1-E93Q), A64T (pEGFP-TRAP1-A64T), and E93Q (pEGFP-TRAP1-E93Q) mutations were created by overlap extension PCR protocol and assembled into XhoI – KpnI site of the Article Title: EPHA2 is a novel cell surface marker of OCT4-positive undifferentiated cells during the differentiation of mouse and human pluripotent stem cells Article Snippet: The PCR reaction was performed with LA-Taq (Takara). .. The amplified fragment was subcloned into AseI and NheI sites of a promoter-less enhanced green fluorescent protein expressing vector, Article Title: Goosecoid promotes pancreatic adenocarcinoma metastasis through TGF-β signaling. Article Snippet: Cell.. Mol.. Biol. (ISSN: 1165-158X) Construct:Article Title: A novel role for CSA in the regulation of nuclear envelope integrity: uncovering a non-canonical function Article Snippet: The next day, transfection of plasmid constructs was performed using Trans-IT 2020 reagent (#MIR 5400; Mirus Bio) following the manufacturer’s protocols. .. 24 h after transfection, experiments were performed. Article Title: Mitochondrial chaperon TNF-receptor- associated protein 1 as a novel apoptotic regulator conferring susceptibility to Pneumocystis jirovecii pneumonia Article Snippet: To generate EGFP-TRAP1 mammalian expression construct (pEGFP-TRAP1), human wild type TRAP1 cDNA was PCR amplified from a clone from the TOH7500-Human-Lentiviral-ORF-Expression-Library (TransOMIC Technologies, Inc.). .. EGFP-TRAP1 plasmid constructs with individual A64T (pEGFP-TRAP1-A64T), E93Q (pEGFP-TRAP1-E93Q), A64T (pEGFP-TRAP1-A64T), and E93Q (pEGFP-TRAP1-E93Q) mutations were created by overlap extension PCR protocol and assembled into XhoI – KpnI site of the Article Title: Cells for characterization of botulinum neurotoxins Article Snippet: .. The intramolecular FRET construct, pMD0031 (FIG. 3A), was constructed in Article Title: Goosecoid promotes pancreatic adenocarcinoma metastasis through TGF-β signaling. Article Snippet: Cell.. Mol.. Biol. (ISSN: 1165-158X) Clone Assay:Article Title: Rit1-TBC1D10B signaling modulates FcγR-mediated phagosome formation in RAW264 macrophages. Article Snippet: .. The fragments were cloned into Article Title: Goosecoid promotes pancreatic adenocarcinoma metastasis through TGF-β signaling. Article Snippet: Cell.. Mol.. Biol. (ISSN: 1165-158X) Mutagenesis:Article Title: Rit1-TBC1D10B signaling modulates FcγR-mediated phagosome formation in RAW264 macrophages. Article Snippet: .. The fragments were cloned into Clinical Proteomics:Article Title: Rit1-TBC1D10B signaling modulates FcγR-mediated phagosome formation in RAW264 macrophages. Article Snippet: .. The fragments were cloned into Membrane:Article Title: Rit1-TBC1D10B signaling modulates FcγR-mediated phagosome formation in RAW264 macrophages. Article Snippet: .. The fragments were cloned into Activity Assay:Article Title: Rit1-TBC1D10B signaling modulates FcγR-mediated phagosome formation in RAW264 macrophages. Article Snippet: .. The fragments were cloned into Generated:Article Title: Rit1-TBC1D10B signaling modulates FcγR-mediated phagosome formation in RAW264 macrophages. Article Snippet: .. The fragments were cloned into other:Article Title: Retinoic acid-induced protein 14 links mechanical forces to Hippo signaling. Article Snippet: To generate the RAI14 guide RNA (gRNA) expressing vector, an annealed gRNA sequence designed using the CHOPCHOP tool (https://chopchop.cbu.uib.no) was inserted into the lentiCRISPR v2 vector (from Addgene, #52961). Expressing:Article Title: EPHA2 is a novel cell surface marker of OCT4-positive undifferentiated cells during the differentiation of mouse and human pluripotent stem cells Article Snippet: The PCR reaction was performed with LA-Taq (Takara). .. The amplified fragment was subcloned into AseI and NheI sites of a promoter-less enhanced green fluorescent protein expressing vector, Sequencing:Article Title: Goosecoid promotes pancreatic adenocarcinoma metastasis through TGF-β signaling. Article Snippet: Cell.. Mol.. Biol. (ISSN: 1165-158X) Negative Control:Article Title: Goosecoid promotes pancreatic adenocarcinoma metastasis through TGF-β signaling. Article Snippet: Cell.. Mol.. Biol. (ISSN: 1165-158X) |