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elution buffer pe  (MACHEREY NAGEL)


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    Structured Review

    MACHEREY NAGEL elution buffer pe
    Elution Buffer Pe, supplied by MACHEREY NAGEL, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+buffer/Elution+Buffer+PE/pmc12562616-177-25-36
    Average 93 stars, based on 3 article reviews
    elution buffer pe - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: Rpv10.2 : A Haplotype Variant of Locus Rpv10 Enables New Combinations for Pyramiding Downy Mildew Resistance Traits in Grapevine
    Article Snippet: Tissue fragments of about a 1–2 cm 2 surface area were collected from the young leaves and stored in pre-cooled 96 deep well plates (Abgene TM 1.2 mL DeepWell Plates, Thermo Fisher Scientific). .. DNA was isolated using an extraction kit (NucleoSpin TM Plant II, Macherey-Nagel) and stored at −20 °C in Elution Buffer PE (5 mM Tris/HCL, pH 8.5). .. DNA was isolated using an extraction kit (NucleoSpin TM Plant II, Macherey-Nagel) and stored at −20 °C in Elution Buffer PE (5 mM Tris/HCL, pH 8.5).

    Extraction:

    Article Title: Rpv10.2 : A Haplotype Variant of Locus Rpv10 Enables New Combinations for Pyramiding Downy Mildew Resistance Traits in Grapevine
    Article Snippet: Tissue fragments of about a 1–2 cm 2 surface area were collected from the young leaves and stored in pre-cooled 96 deep well plates (Abgene TM 1.2 mL DeepWell Plates, Thermo Fisher Scientific). .. DNA was isolated using an extraction kit (NucleoSpin TM Plant II, Macherey-Nagel) and stored at −20 °C in Elution Buffer PE (5 mM Tris/HCL, pH 8.5). .. DNA was isolated using an extraction kit (NucleoSpin TM Plant II, Macherey-Nagel) and stored at −20 °C in Elution Buffer PE (5 mM Tris/HCL, pH 8.5).

    Serial Dilution:

    Article Title: Temporal colonization of macadamia tree roots by <i>Phytophthora cinnamomi</i> based on a newly developed quantitative real‐time PCR assay
    Article Snippet: The DNA concentration was determined using a Qubit 4.0 fluorometer (Thermo Fisher Scientific Inc.). .. A five-fold serial dilution range was made using the Nucleospin elution buffer PE (Macherey-Nagel) to obtain a standard curve ranging in concentration from 54 to 5.43−6 ng/μL. .. Each qPCR contained 1× Sensi Probe II mix (Meridian Bioscience), 0.4 μM of each primer, 0.2 μM probe and 2 μL genomic DNA in a total volume of 20 μL.

    Concentration Assay:

    Article Title: Temporal colonization of macadamia tree roots by <i>Phytophthora cinnamomi</i> based on a newly developed quantitative real‐time PCR assay
    Article Snippet: The DNA concentration was determined using a Qubit 4.0 fluorometer (Thermo Fisher Scientific Inc.). .. A five-fold serial dilution range was made using the Nucleospin elution buffer PE (Macherey-Nagel) to obtain a standard curve ranging in concentration from 54 to 5.43−6 ng/μL. .. Each qPCR contained 1× Sensi Probe II mix (Meridian Bioscience), 0.4 μM of each primer, 0.2 μM probe and 2 μL genomic DNA in a total volume of 20 μL.

    Lysis:

    Article Title: Genome Characterization of Stelechocarpus burahol (Blume) Hook.f. & Thomson “Kepel” and Exploration of Phytochemicals from Water and Ethanolic Extracts of Leaves and Fruits
    Article Snippet: PCR master mix reagent was purchased from iNtRON Biotechnology Company, Yeonggi-do, Republic of Korea. .. NucleoSpin filters, spin column, and Plant II reagent kit including lysis buffers PL1 and PL2, precipitation buffer PL3, PCR buffer, washing buffers PW1 and PW2, elution buffer PE, and ribonuclease A (RNase A) were obtained from Macherey-Nagel GmbH & Co. KG, Duren, Germany. .. Nucleic acid staining dye “SafeView TM FireRed dye” was purchased from Applied Biological Materials Inc., Richmond, BC, Canada.

    Polymerase Chain Reaction:

    Article Title: Genome Characterization of Stelechocarpus burahol (Blume) Hook.f. & Thomson “Kepel” and Exploration of Phytochemicals from Water and Ethanolic Extracts of Leaves and Fruits
    Article Snippet: PCR master mix reagent was purchased from iNtRON Biotechnology Company, Yeonggi-do, Republic of Korea. .. NucleoSpin filters, spin column, and Plant II reagent kit including lysis buffers PL1 and PL2, precipitation buffer PL3, PCR buffer, washing buffers PW1 and PW2, elution buffer PE, and ribonuclease A (RNase A) were obtained from Macherey-Nagel GmbH & Co. KG, Duren, Germany. .. Nucleic acid staining dye “SafeView TM FireRed dye” was purchased from Applied Biological Materials Inc., Richmond, BC, Canada.



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    Servicebio Inc annexin v pe binding buffer
    BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via <t>Annexin</t> <t>V-PE/7-AAD</t> staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    Qiagen pe 19065
    BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via <t>Annexin</t> <t>V-PE/7-AAD</t> staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    Image Search Results


    BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via Annexin V-PE/7-AAD staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Veterinary Research

    Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

    doi: 10.1186/s13567-026-01729-7

    Figure Lengend Snippet: BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via Annexin V-PE/7-AAD staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

    Techniques: Infection, Expressing, Western Blot, Staining, Control

    The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Veterinary Research

    Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

    doi: 10.1186/s13567-026-01729-7

    Figure Lengend Snippet: The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

    Techniques: Infection, Biomarker Discovery, Flow Cytometry, Endpoint Dilution Assay, Expressing, Immunofluorescence, Staining, Knockdown, Fluorescence