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pe buffer  (Qiagen)


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    Structured Review

    Qiagen pe buffer
    Pe Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 583 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+buffer/Buffer+PE/pmc13133277-252-13-15
    Average 97 stars, based on 583 article reviews
    pe buffer - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Ancient mitogenomes reveal unexpected diversity in Madagascar’s extinct hippopotamuses
    Article Snippet: Samples were then centrifuged and the supernatant mixed with 13 mL of binding buffer (12.6 mL of Qiagen PB buffer, 6.5 μL of Tween-20, and 390 μL of sodium acetate). .. The mixture was passed through a Qiagen MinElute column using a Zymo 15 mL reservoir, washed twice with 700 μL Qiagen PE buffer, dried by centrifugation, and eluted twice in 30 μL TE buffer. ..

    Isolation:

    Article Title: BRD4 recruitment desilences transcription without erasure or depletion of repressive chromatin
    Article Snippet: .. DNA was isolated using Qiagen DNA cleanup buffers PB (19066) and PE (19065). ..

    other:

    Article Title: Protocol for integrative analysis of transcription factor-nucleosome interactions using SeEN-seq and cryo-EM structure determination.
    Article Snippet: Carefully open the glass plates and stain the gel in 100 mL of deionized water with 10 μL of SYBR Gold (Invitrogen) for 10 min. 35.

    Purification:

    Article Title: Design and characterization of peptide-responsive biosensors
    Article Snippet: PCR products were purified using the Qiagen QIAquick columns and pooled with 5x the volume of binding buffer (Buffer PB, Qiagen) was added to the reactions. .. The volumes were run through purification columns (16 PCR reactions’ worth of volume per column) and washed with 2x with Buffer PE (Qiagen) using a manifold system. .. Columns are then spun down at maximum speed for 1 min to remove residual wash buffer, then rehydrated with 30 μL of MiliQ water per column, and spun again in a fresh Eppendorf tube to collect.

    Article Title: DMS-MaPseq and DREEM Analyses Implicate the Critical Role of RNA Structural Dynamics in Turnip Yellow Mosaic Virus Pathogenicity.
    Article Snippet: .. The PCR products were gel purified using Qiagen QG (cat#19063) and PE (cat#19065) buffer. .. The in vitro transcription reaction containing 250 ng templates, 1 μL of 100 m m ATG, 1 μL of 100 m CTP, 1 μL of 100 m GTP, 0.4 μL of 100 m UTP, 0.8 μL of 50 m biotin-16-UTP (Lucigen), 1.5 μL T7 RNA polymerase (lab purified), and 1 μL Superase-In RNase inhibitor (Invitrogen cat#AM2696) in a total of 20 μL was incubated at 37◦C 13 of 18 tive C om m ons L icense o a w R o 1 1 m R e 4 T S p f R 1 R b t m 5 T d w k r 4 D 5 d S I l 4 T M 4 R t r T f q o 9 q t 1 nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.75614 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [12/05/2026].

    Polymerase Chain Reaction:

    Article Title: Design and characterization of peptide-responsive biosensors
    Article Snippet: PCR products were purified using the Qiagen QIAquick columns and pooled with 5x the volume of binding buffer (Buffer PB, Qiagen) was added to the reactions. .. The volumes were run through purification columns (16 PCR reactions’ worth of volume per column) and washed with 2x with Buffer PE (Qiagen) using a manifold system. .. Columns are then spun down at maximum speed for 1 min to remove residual wash buffer, then rehydrated with 30 μL of MiliQ water per column, and spun again in a fresh Eppendorf tube to collect.

    Article Title: DMS-MaPseq and DREEM Analyses Implicate the Critical Role of RNA Structural Dynamics in Turnip Yellow Mosaic Virus Pathogenicity.
    Article Snippet: .. The PCR products were gel purified using Qiagen QG (cat#19063) and PE (cat#19065) buffer. .. The in vitro transcription reaction containing 250 ng templates, 1 μL of 100 m m ATG, 1 μL of 100 m CTP, 1 μL of 100 m GTP, 0.4 μL of 100 m UTP, 0.8 μL of 50 m biotin-16-UTP (Lucigen), 1.5 μL T7 RNA polymerase (lab purified), and 1 μL Superase-In RNase inhibitor (Invitrogen cat#AM2696) in a total of 20 μL was incubated at 37◦C 13 of 18 tive C om m ons L icense o a w R o 1 1 m R e 4 T S p f R 1 R b t m 5 T d w k r 4 D 5 d S I l 4 T M 4 R t r T f q o 9 q t 1 nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.75614 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [12/05/2026].



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    BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via <t>Annexin</t> <t>V-PE/7-AAD</t> staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    Image Search Results


    BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via Annexin V-PE/7-AAD staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Veterinary Research

    Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

    doi: 10.1186/s13567-026-01729-7

    Figure Lengend Snippet: BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via Annexin V-PE/7-AAD staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

    Techniques: Infection, Expressing, Western Blot, Staining, Control

    The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Veterinary Research

    Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

    doi: 10.1186/s13567-026-01729-7

    Figure Lengend Snippet: The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

    Techniques: Infection, Biomarker Discovery, Flow Cytometry, Endpoint Dilution Assay, Expressing, Immunofluorescence, Staining, Knockdown, Fluorescence