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G Biosciences tissue-pe lb lysis buffer
Tissue Pe Lb Lysis Buffer, supplied by G Biosciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Lysis:

Article Title: High fat diet causes distinct aberrations in the testicular proteome.
Article Snippet: In addition, Immunofluorescence and Terminal deoxynucleotidyl transferase staining (TUNEL) staining (Roche, UK) performed with Zeiss 510 confocal microscopy for visualisation (see supplementary methods). .. Whole mouse testes were disrupted in 1 ml of Tissue PE lysis buffer (G Biosciences) and protease cocktail inhibitor (Sigma) using Qiagen TissueLyser. .. After protein quantification with appropriate standards (Biorad™ DC Protein Assay), 10 μg protein extract was separated using SDSPAGE.

Article Title: Therapeutic nanoparticles and methods of use thereof
Article Snippet: .. Western Blot Frozen tumor sections were thawed and homogenized in tissue protein extraction lysis buffer (Tissue-PE LB from G-Biosciences, St Louis, Mo., USA) along with 1 mM phenylmethylsulfonyl fluoride (PMSF) and proteinase inhibitor cocktails (Sigma, St Louis, Mo., USA). .. Western Blot Frozen tumor sections were thawed and homogenized in tissue protein extraction lysis buffer (Tissue-PE LB from G-Biosciences, St Louis, Mo., USA) along with 1 mM phenylmethylsulfonyl fluoride (PMSF) and proteinase inhibitor cocktails (Sigma, St Louis, Mo., USA).

Article Title: Expression and localization of insulin-like growth factor system in corpus luteum during different stages of estrous cycle in water buffaloes (Bubalus bubalis) and the effect of insulin-like growth factor I on production of vascular endothelial growth factor and progesterone in luteal cells cultured in vitro.
Article Snippet: This study investigated the expression and localization of insulin-like growth factor (IGF) system at different stages of buffalo CL and the role of IGF-I in stimulating vascular endothelial growth factor (VEGF) and progesterone (P4) production in cultured luteal cells.. The mRNA expression of IGF system, VEGF, steroidogenic acute regulatory protein, P450scc, and hydroxysteroid dehydrogenase (HSD) was investigated by quantitative real-time polymerase chain reaction (PCR).. Protein expression of IGF was demonstrated by Western blot and localization by immunohistochemistry.

Article Title: Voluntary exercise training attenuated the middle-aged maturity-induced cardiac apoptosis.
Article Snippet: Aims: Voluntary exercise training has cardioprotective effects in humans, but the underlying mechanism is unknown.. This research was done to estimate the effect of voluntary exercise training to attenuate middle-aged maturity-induced cardiac apoptosis.. Materials and methods: The study was designed to divide 64 male mice randomly into four groups, consisting of a 9-month sedentary pre-middle-aged group (9M), 15-month sedentary middle-aged group (15M), and two exercise groups using a voluntary wheel running respectively (9M+EX, 15M+EX).

Article Title: Co-administration of nalbuphine attenuates the morphine-induced anxiety and dopaminergic alterations in morphine-withdrawn rats.
Article Snippet: Introduction The classical effects of exogenous opioids, such as morphine, are predominantly mediated through μ-opioid receptors.. The chronic use of morphine induces anxiety-like behavior causing functional changes in the mesolimbic dopaminergic system.. The mixed μ/κ-agonist, nalbuphine, used either as an analgesic or as an adjuvant with morphine, produces different and opposite effects.

Article Title: Extract of Ganoderma formosanum Mycelium as a Highly Potent Tyrosinase Inhibitor
Article Snippet: .. Total protein was harvested with Tissue PE LB TM lysis buffer (G-BIOSCIENCES, Maryland Heights, MO, USA) supplemented with protease inhibitor and clarified by centrifugation at 4 °C with 10,000 × g for 5 mins. .. The amount of protein was quantified by Bradford assay using bovine serum albumin as a standard (Bio-Rad), then 50 μg of total protein reacted with 1 mM L -3, 4-dihydroxyphenylalanine ( L -DOPA) (Sigma) and incubated at 37 °C for 1 hour.

Article Title: Regulation of systemic tissue injury by coagulation inhibitors in B6.MRL/lpr autoimmune mice.
Article Snippet: Accepted Manuscript Regulation of systemic tissue injury by coagulation inhibitors in B6.MRL/lpr autoimmune mice C. Moratz, R. Robbins, J. Eickhoff, J. Edison, H. Lui, S. Peng PII: S1521-6616(18)30263-8 DOI: doi:10.1016/j.clim.2018.08.010 Reference: YCLIM 8091 To appear in: Clinical Immunology Received date: 30 April 2018 Revised date: 23 August 2018 Accepted date: 23 August 2018 Please cite this article as: C. Moratz, R. Robbins, J. Eickhoff, J. Edison, H. Lui, S. Peng , Regulation of systemic tissue injury by coagulation inhibitors in B6.MRL/lpr autoimmune mice.. Yclim (2018), doi:10.1016/j.clim.2018.08.010 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Western Blot:

Article Title: Therapeutic nanoparticles and methods of use thereof
Article Snippet: .. Western Blot Frozen tumor sections were thawed and homogenized in tissue protein extraction lysis buffer (Tissue-PE LB from G-Biosciences, St Louis, Mo., USA) along with 1 mM phenylmethylsulfonyl fluoride (PMSF) and proteinase inhibitor cocktails (Sigma, St Louis, Mo., USA). .. Western Blot Frozen tumor sections were thawed and homogenized in tissue protein extraction lysis buffer (Tissue-PE LB from G-Biosciences, St Louis, Mo., USA) along with 1 mM phenylmethylsulfonyl fluoride (PMSF) and proteinase inhibitor cocktails (Sigma, St Louis, Mo., USA).

Article Title: Regulation of systemic tissue injury by coagulation inhibitors in B6.MRL/lpr autoimmune mice.
Article Snippet: Accepted Manuscript Regulation of systemic tissue injury by coagulation inhibitors in B6.MRL/lpr autoimmune mice C. Moratz, R. Robbins, J. Eickhoff, J. Edison, H. Lui, S. Peng PII: S1521-6616(18)30263-8 DOI: doi:10.1016/j.clim.2018.08.010 Reference: YCLIM 8091 To appear in: Clinical Immunology Received date: 30 April 2018 Revised date: 23 August 2018 Accepted date: 23 August 2018 Please cite this article as: C. Moratz, R. Robbins, J. Eickhoff, J. Edison, H. Lui, S. Peng , Regulation of systemic tissue injury by coagulation inhibitors in B6.MRL/lpr autoimmune mice.. Yclim (2018), doi:10.1016/j.clim.2018.08.010 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Protein Extraction:

Article Title: Therapeutic nanoparticles and methods of use thereof
Article Snippet: .. Western Blot Frozen tumor sections were thawed and homogenized in tissue protein extraction lysis buffer (Tissue-PE LB from G-Biosciences, St Louis, Mo., USA) along with 1 mM phenylmethylsulfonyl fluoride (PMSF) and proteinase inhibitor cocktails (Sigma, St Louis, Mo., USA). .. Western Blot Frozen tumor sections were thawed and homogenized in tissue protein extraction lysis buffer (Tissue-PE LB from G-Biosciences, St Louis, Mo., USA) along with 1 mM phenylmethylsulfonyl fluoride (PMSF) and proteinase inhibitor cocktails (Sigma, St Louis, Mo., USA).

Article Title: Voluntary exercise training attenuated the middle-aged maturity-induced cardiac apoptosis.
Article Snippet: Aims: Voluntary exercise training has cardioprotective effects in humans, but the underlying mechanism is unknown.. This research was done to estimate the effect of voluntary exercise training to attenuate middle-aged maturity-induced cardiac apoptosis.. Materials and methods: The study was designed to divide 64 male mice randomly into four groups, consisting of a 9-month sedentary pre-middle-aged group (9M), 15-month sedentary middle-aged group (15M), and two exercise groups using a voluntary wheel running respectively (9M+EX, 15M+EX).

Article Title: Co-administration of nalbuphine attenuates the morphine-induced anxiety and dopaminergic alterations in morphine-withdrawn rats.
Article Snippet: Introduction The classical effects of exogenous opioids, such as morphine, are predominantly mediated through μ-opioid receptors.. The chronic use of morphine induces anxiety-like behavior causing functional changes in the mesolimbic dopaminergic system.. The mixed μ/κ-agonist, nalbuphine, used either as an analgesic or as an adjuvant with morphine, produces different and opposite effects.

Article Title: Regulation of systemic tissue injury by coagulation inhibitors in B6.MRL/lpr autoimmune mice.
Article Snippet: Accepted Manuscript Regulation of systemic tissue injury by coagulation inhibitors in B6.MRL/lpr autoimmune mice C. Moratz, R. Robbins, J. Eickhoff, J. Edison, H. Lui, S. Peng PII: S1521-6616(18)30263-8 DOI: doi:10.1016/j.clim.2018.08.010 Reference: YCLIM 8091 To appear in: Clinical Immunology Received date: 30 April 2018 Revised date: 23 August 2018 Accepted date: 23 August 2018 Please cite this article as: C. Moratz, R. Robbins, J. Eickhoff, J. Edison, H. Lui, S. Peng , Regulation of systemic tissue injury by coagulation inhibitors in B6.MRL/lpr autoimmune mice.. Yclim (2018), doi:10.1016/j.clim.2018.08.010 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

other:

Article Title: Therapeutic nanoparticles and methods of use thereof
Article Snippet: followed by a DyLight 488-labeled goat polyclonal secondary antibody to Rabbit IgG (Abcam, Cambridge, Mass.).

Extraction:

Article Title: Expression and localization of insulin-like growth factor system in corpus luteum during different stages of estrous cycle in water buffaloes (Bubalus bubalis) and the effect of insulin-like growth factor I on production of vascular endothelial growth factor and progesterone in luteal cells cultured in vitro.
Article Snippet: This study investigated the expression and localization of insulin-like growth factor (IGF) system at different stages of buffalo CL and the role of IGF-I in stimulating vascular endothelial growth factor (VEGF) and progesterone (P4) production in cultured luteal cells.. The mRNA expression of IGF system, VEGF, steroidogenic acute regulatory protein, P450scc, and hydroxysteroid dehydrogenase (HSD) was investigated by quantitative real-time polymerase chain reaction (PCR).. Protein expression of IGF was demonstrated by Western blot and localization by immunohistochemistry.

Protease Inhibitor:

Article Title: Expression and localization of insulin-like growth factor system in corpus luteum during different stages of estrous cycle in water buffaloes (Bubalus bubalis) and the effect of insulin-like growth factor I on production of vascular endothelial growth factor and progesterone in luteal cells cultured in vitro.
Article Snippet: This study investigated the expression and localization of insulin-like growth factor (IGF) system at different stages of buffalo CL and the role of IGF-I in stimulating vascular endothelial growth factor (VEGF) and progesterone (P4) production in cultured luteal cells.. The mRNA expression of IGF system, VEGF, steroidogenic acute regulatory protein, P450scc, and hydroxysteroid dehydrogenase (HSD) was investigated by quantitative real-time polymerase chain reaction (PCR).. Protein expression of IGF was demonstrated by Western blot and localization by immunohistochemistry.

Article Title: Voluntary exercise training attenuated the middle-aged maturity-induced cardiac apoptosis.
Article Snippet: Aims: Voluntary exercise training has cardioprotective effects in humans, but the underlying mechanism is unknown.. This research was done to estimate the effect of voluntary exercise training to attenuate middle-aged maturity-induced cardiac apoptosis.. Materials and methods: The study was designed to divide 64 male mice randomly into four groups, consisting of a 9-month sedentary pre-middle-aged group (9M), 15-month sedentary middle-aged group (15M), and two exercise groups using a voluntary wheel running respectively (9M+EX, 15M+EX).

Article Title: Co-administration of nalbuphine attenuates the morphine-induced anxiety and dopaminergic alterations in morphine-withdrawn rats.
Article Snippet: Introduction The classical effects of exogenous opioids, such as morphine, are predominantly mediated through μ-opioid receptors.. The chronic use of morphine induces anxiety-like behavior causing functional changes in the mesolimbic dopaminergic system.. The mixed μ/κ-agonist, nalbuphine, used either as an analgesic or as an adjuvant with morphine, produces different and opposite effects.

Article Title: Extract of Ganoderma formosanum Mycelium as a Highly Potent Tyrosinase Inhibitor
Article Snippet: .. Total protein was harvested with Tissue PE LB TM lysis buffer (G-BIOSCIENCES, Maryland Heights, MO, USA) supplemented with protease inhibitor and clarified by centrifugation at 4 °C with 10,000 × g for 5 mins. .. The amount of protein was quantified by Bradford assay using bovine serum albumin as a standard (Bio-Rad), then 50 μg of total protein reacted with 1 mM L -3, 4-dihydroxyphenylalanine ( L -DOPA) (Sigma) and incubated at 37 °C for 1 hour.

Centrifugation:

Article Title: Extract of Ganoderma formosanum Mycelium as a Highly Potent Tyrosinase Inhibitor
Article Snippet: .. Total protein was harvested with Tissue PE LB TM lysis buffer (G-BIOSCIENCES, Maryland Heights, MO, USA) supplemented with protease inhibitor and clarified by centrifugation at 4 °C with 10,000 × g for 5 mins. .. The amount of protein was quantified by Bradford assay using bovine serum albumin as a standard (Bio-Rad), then 50 μg of total protein reacted with 1 mM L -3, 4-dihydroxyphenylalanine ( L -DOPA) (Sigma) and incubated at 37 °C for 1 hour.



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BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via <t>Annexin</t> <t>V-PE/7-AAD</t> staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via Annexin V-PE/7-AAD staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Journal: Veterinary Research

Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

doi: 10.1186/s13567-026-01729-7

Figure Lengend Snippet: BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via Annexin V-PE/7-AAD staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

Techniques: Infection, Expressing, Western Blot, Staining, Control

The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: Veterinary Research

Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

doi: 10.1186/s13567-026-01729-7

Figure Lengend Snippet: The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

Techniques: Infection, Biomarker Discovery, Flow Cytometry, Endpoint Dilution Assay, Expressing, Immunofluorescence, Staining, Knockdown, Fluorescence